Beta-Lactamase Production Among Uropathogens in Patients Attending Some Hospitals in Kano, Nigeria
International Journal of Biomedical Materials Research
Volume 4, Issue 3, December 2016, Pages: 58-62
Received: Aug. 19, 2016; Accepted: Dec. 2, 2016; Published: Jan. 4, 2017
Views 2789      Downloads 104
Hamza Sule, Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Bayero University, Kano, Nigeria
Abdulhadi Sale Kumurya, Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Bayero University, Kano, Nigeria
Article Tools
Follow on us
Ability of bacteria to withstand antibiotic therapy is often associated with resistance. Certain bacteria have the ability to produce some enzymes, like Beta–Lactamase, which confers resistance to some groups of antibiotics like penicillins and cephalosporins. The study focused on producers of these enzymes among uropathogens. A total of one hundred and forteen isolates (114) were screened, out of which seventy one 71 (62.3%) were found positive for the enzymes. Eschericia coli had the highest positivity rate 40 (56.3%). Followed by Klebsiella spp., Staphylococcus aureus, Staphylociccus saprophyticcus, Proteus mirabilis, and Pseudomonas aeruginosa with 15 (21.1%), 7 (10.0%), 5 (7.0%), 3 (4.2%), and 1 (1.4%) respectively. In the same vein, it was discovered that, isolates of females’ origin, produced more of the enzymes than those of their males counterpart. This was indicated by the production of the enzyme by at least one isolate in each of the species isolated from female samples while in males, some species did not even produce any of the enzymes. Age-wise distribution of the enzymes showed that, 21- 30, and 31-40 age groups had all the isolates producing the enzymes in varying frequencies. Only Pseudomonas aeruginosa that produced its enzyme outside these age groups. Isolates of females origin had more of the enzymes produced in all the species compared to those from males, with ratios of; 26:14, 11:4, 5:2, 3:2, 2:1, and 1:0 for, E coli, Klebsiella species, Staphylococcus aureus, Staphylococcus saprophyticus, Proteus mirabilis, and Pseudomonas aeruginosa respectively.
Urinary Tract Infections, Uropathogens, Beta-Lactamase, Kano
To cite this article
Hamza Sule, Abdulhadi Sale Kumurya, Beta-Lactamase Production Among Uropathogens in Patients Attending Some Hospitals in Kano, Nigeria, International Journal of Biomedical Materials Research. Vol. 4, No. 3, 2016, pp. 58-62. doi: 10.11648/j.ijbmr.20160403.18
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abigail, A. Dixie; W. (2005). Bacterial Pathogenesis: A Molecular approach, Washington DC ASM Press,. 2005.
Alabi, O. S., Onyenwe, N. E. Satoye, K. A. and Adeleke, O. E. (2014). Prevalence of extended-spectrum β-lactamase producing isolates from asymptomatic bacteriuria among students in a tertiary institution in Ibadan, Nigeria. Nature and Science 12 (4): 113.
Al-Issa, M. (2009). Urinary tract infection among pregnant women in north Jordan. Middle East J Fam Med., 7: 10-4.
Ben–Ami, R., Rodriguez–Bafio, J., Calbo E. S. And Arslan, H. A. (2009). Multinational survey of risk factors for infection with extended spectrum beta lactamasesmproducing Enterobacteriaceae in non hospitalized patients. 5 (1): 49–51.
Bush, K., Jacoby, G. A. and Medeiros, A. A. (1995). A Functional Classification Scheme for β- lactamases and its Correlation with Molecular Structure. Antimicrob. Agents Chemother. 39: 1211-33.
Chaliha, C. Stanton, S. L., (2002). Urological problems in pregnancy. BJU Int., 89 (5): 469-76. To remove
Chander and Shrestha, (2013). Prevalence of extended spectrum beta lactamase producing Escherichia coli and Klebsiella pneumoniae urinary isolates in a tertiary care hospital in Kathmandu, Nepal. BMC Research Notes 6: 487.
Cheesebrough, M. (2000). Distric Laboratory Practice in Tropical Countries Low Price ed. Chambridge University Press. Pp. 434.
Cox, C. E. (1988). Nosocomial urinary tract infections. Urol, 32 (3): 210–215.
Datta, N. and Kontomichalou, P. (1965)."Penicillinase synthesis controlled by infectious R factors in Enterobacteriaceae." Nature 208: 239-41.
Foxman, B., Barlow, R., D’Arcy, H., Gillespie, B., and Sobel, J. D. (2000). Urinary tract infection: Self-reported incidence and associated costs. Ann Epidemiol; 10 (8): 509-15.
Gaurav, Dalela (2012). PREV. of Extended Spectrum Beta-Lactamase (ESBL) Producers among Gram Negative Bacilli from Various Clinical Isolates in a Tertiary Care Hospital at Jhalawar, Rajasthan, India. Journal of Clinical and Diagnostic Research. 6 (2): 182-187.
Gonzalez, C. M., and Schaeffer, A. J. (1999). Treatment of urinary tract infection: what’s old, what’s new, and what works. World J Urol.; 17 (6): 372–382.
Kapur, A., Ahmed, M. S., and John, S. (2015). Prevalence of Extended-Spectrum Beta-Lactamase-Producing Pathogens From Urinary Tract Infected Samples and Their Sensitivity Pattern Against Withania somnifera. Int J Infect. 2 (1): e22664.
Livermore, D. M. and Woodford, N. (2004). Laboratory detection and reporting of bacteria with extended spectrum betalactamases. Antibiotic resistance monitoring and reference laboratory, specialist and reference. Microbiology division, Health Protection Agency -Colindale, London.
Maina, D., Makau, P., Nyerere, A. and Revathi, G. (2013). Antimicrobial resistance patterns in extended-spectrum β-lactamase producing Escherichia coli and Klebsiella pneumoniae isolates in a private tertiary hospital, Kenya. Microbiology Discovery, 1 (5): 1-4.
Mansour, A. Manijeh, M. and Zohreh, P. (2009). Study of bacteria isolated from urinary tract infections and determination of their susceptibility to antibiotics. Jundishapur J Microbiol; 2 (3): 118–23.
Mackey, McCartney, (1996). Tropical Medical Micrbiology 14th ed. Churchill Livingstone. London. Pp. 978.
Rai, G. Upretin, H., Rai, S., Saha, K. and Shrestha, R. (2008). Nepal Med Coll J. 10, 86.
Romero, L., López, L., Rodríguez-Baño, J., Ramón, Hernández, J., Martínez-Martínez, L., and Pascual, A. (2005). Long-term study of the frequency of Escherichia coli and Klebsiella pneumoniae isolates producing extended-spectrum beta-lactamases. Clin Microbiol Infect.; 11 (8): 625-31.
Rupp, M. E., and Fey, P. D. (2003). Extended spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae: Considerations for diagnosis, prevention and drug treatment. Drugs; 63 (4): 353-65.
Samaha-Kfoury, J. N., Araj, G. F. (2003). Recent developments in β-lactamases and extended spectrum β-lactamases. BMJ; 327: 1209-13.
Senkas-Dzidic, J., and Suskovic., B., (2008). Kos, Food Technolo. Biotechnolo., 46, 11.
Thapa, R., Pramila, L., Megha, Raj, B., and Ganesh, P. A. (2015). Prevalence of extended spectrum beta lactamase producing uropathogens in pregnant women. Asian J Pharm Clin Res, 8 (1), 2015, 207-210.
Ullah, F., Malik, S. A, Ahmed J. (2009): Antibiotic susceptibility pattern and ESBL prevalence in nosocomial Escherichia coli from urinary tract infections in Pakistan. Afr J Biotechnol, 8 (16): 3921–3926.
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
Tel: (001)347-983-5186