Molecular Diversity of Cipadessa baccifera (Roth) Miq Based on Inter Simple Sequence Repeats
International Journal of Biomedical Materials Research
Volume 5, Issue 1, February 2017, Pages: 1-4
Received: Oct. 31, 2016; Accepted: Nov. 15, 2016; Published: Jan. 13, 2017
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Authors
E. Jebarubi, Postgraduate and Research Department of Botany, St. Xavier’s College (Autonomous), Palayamkottai, Tirunelveli, Tamil Nadu, India
S. Christopher Patrick Kiladi, Postgraduate and Research Department of Botany, St. Xavier’s College (Autonomous), Palayamkottai, Tirunelveli, Tamil Nadu, India
T. Leon Stephan Raj, Postgraduate and Research Department of Botany, St. Xavier’s College (Autonomous), Palayamkottai, Tirunelveli, Tamil Nadu, India
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Abstract
Medicinal plants are nature’s gifts and used in different traditional medicinal systems of India. Most of the medicinal plants are obtained from forest without proper management and knowledge of collection. For the authentication of medicinal plants from the substituted materials, a genetic diversity study is necessary. In the present investigation molecular diversity of Cipadessa baccifera was carried out using dominant ISSR (inter simple sequence repeats) markers in four different accessions of Western Ghats of South India. Five primers were selected from a total of ten primers based on the reproducibility of the bands. The number of polymorphic loci was 13 and the percentage of polymorphic loci was 56.52. The genetic distance among the populations ranged from 0.0910 to 0.3629 and the genetic identity ranged from 0.6087 to 0.9130. The overall observed and effective number of alleles was about 1.5652 and 1.3913. Nei’s overall genetic diversity and Shannon information index were 0.2283 and 0.3349. The dendrogram was constructed based on the UPGMA method and the clusters formed depending upon the genetic characters.
Keywords
Molecular Diversity, Inter Simple Sequence Repeats (ISSR), Cipadessa baccifera, Medicinal Plants
To cite this article
E. Jebarubi, S. Christopher Patrick Kiladi, T. Leon Stephan Raj, Molecular Diversity of Cipadessa baccifera (Roth) Miq Based on Inter Simple Sequence Repeats, International Journal of Biomedical Materials Research. Vol. 5, No. 1, 2017, pp. 1-4. doi: 10.11648/j.ijbmr.20170501.11
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Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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