Anticoagulant Activity of Rhamnan Sulfate Isolated from Commercially Cultured <i>Monostroma nitidum</i>

Youichi Yamashiro; Masahiro Nakamura; Takuya Yogi; Takeshi Teruya; Teruko Konishi; Shuntoku Uechi; Masakuni Tako

doi:doi:10.11648/j.ijbmr.20170503.12

| Peer-Reviewed

Anticoagulant Activity of Rhamnan Sulfate Isolated from Commercially Cultured Monostroma nitidum

Youichi Yamashiro, Masahiro Nakamura, Takuya Yogi, Takeshi Teruya, Teruko Konishi, Shuntoku Uechi, Masakuni Tako

Published in International Journal of Biomedical Materials Research (Volume 5, Issue 3)

Received: 11 April 2017 Accepted: 2 May 2017 Published: 21 June 2017

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

The green seaweed, Monostroma nitidum, is widespread in Japan. In Okinawa Prefecture, the production of seaweed is performed using culture-nets that are seeded artificially. Algae contain a soluble polysaccharide, rhamnan sulfate. To estimate its applicability, the anticoagulant activity of rhamnan sulfate was investigated. Rhamanan sulfate was fractionated by ion-exchange chromatography on a DEAE-sepharose column, and two fractions (A and B) were obtained. Partially hydrolyzed rhamnan sulfates with different molecular mass (C1, C2 and C3) were also prepared. The activated partial thromboplastin time (APTT) test, prothrombin time (PT) and thrombin time (TT) were applied using human plasma and compared with standard heparin (174 units/mg). The native rhamnan sulfate (molecular mass, 630 kDa; sulfuric acid content, 22.7%), fraction A (12.4%) and fraction B (27.8%) showed approximately 73% APTT activity in comparison with that of standard heparin, but fraction C2 (molecular mass, 450 kDa) had a higher activity than that of the standard (107%). On the other hand, in the PT assay, all fractions except fraction C2 and C3 (370 kDa) showed higher activity approximately 120-155% greater than that of standard heparin. The TT activity of rhamnan sulfate depended on the sulfate content, and that of fraction B, which has high sulfuric acid content (27.8%), was 135-173% greater than that of heparin. The sulfate groups of L-rhamnosyl residues and carboxyl group of D-glucuronosyl residue on the trisaccharide side chains of the rhamnan sulfate might interact strongly with the active site of thrombin molecules. The results and discussion suggested that rhamnan sulfate from commercially cultured Monostroma nitidum could be a potential anticoagulant polysaccharide.

Published in	International Journal of Biomedical Materials Research (Volume 5, Issue 3)
DOI	10.11648/j.ijbmr.20170503.12
Page(s)	37-43
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Monostroma nitidum, Commercially Cultured, Seaweed Polysaccharide, Rhamnan Sulfate, Anticoagulant Activity

References

[1]	Tako, M. (1994) Isolation of an agar from Gracilaria blodgettii and its gelling properties. Journal of Applied Glycoscience, 41, 305-311.
[2]	Tako, M., Higa, M., Medoruma, K. and Nakasone, Y. (1999) A highly methylated agar from red seaweed Gracilaria arcuata. Botanica Marina, 42, 513-517.
[3]	Tako, M., Uehara, M., Kawashima, Y., Chinen, I. and Hongo, F. (1996) Isolation and Identification of fucoidan from Okinawamozuku (Cladosiphon okamuranus TOKIDA). Journal of Applied Glycoscience, 34, 143-148.
[4]	Tako, M., Nakada, T. and Hongo, F. (1999) Chemical characterization of fucoidan from commercially cultured Nemacystus decipiens. Bioscience, Biotechnology, and Biochemistry, 53, 1813-1815.
[5]	Tako, M., Yoza, E. and Tohma, S. (2000) Chemical characterization of acetylfucoidan and alginate from commercially cultured Cladosiphon okamuranus. Botanica Marina, 43, 393-398.
[6]	Shiroma, R., Uechi, S., Taira, T., Ishihara, W., Tawata, S and Tako, M. (2003) Isolation and characterization of fucoidan from Hizikia fusiformis. Jouranl of Applied Glycoscience, 50, 361-365.
[7]	Shiroma, R., Konishi, T. and Tako, M. (2008) Structural study of fucoidan from the brown seaweed Hizikia fusiformis. Food Science and Technology Research, 14, 176-182.
[8]	Tako, M., Kiyuna, S. and Hongo, F. (2001) Isolation and characterization of alginic acid from commercially cultured Nemacystus decipiens. Bioscience, Biotechnology and Biochemistry, 65, 654-657.
[9]	Shiroma, R., Uechi, S., Tawata, S. and Tako, M. (2007) Isolation and characterization of alginate from Hizikia fusiformis and preparation of its oligosaccharides. Journal of Applied Glycoscience, 54, 85-90.
[10]	Qi, Z.-Q., Tako, M. and Toyama, S. (1997) Chemical characterization of κ-carrageenan of Ibaranori (Hypnea charoides). Journal of Applied Glycoscience, 44, 137-142.
[11]	Lin, L.-H., Tako, M., Hongo, F. (2000) Isolation and characterization of ι-carrageenan isolated from Eucheuma serra. Journal of Applied Glycoscience, 47, 303-310.
[12]	Nakamura, M., Yamashiro, Y., Konishi, T., Hanashiro, I. and Tako, M. (2011) Structural characteristics of rhamnan sulfate from commercially cultured Monostroma nitidum. Nippon Shokuhin Kagaku Kogaku Kaishi, 58, 245-251.
[13]	Tako, M., Tamanaha, M., Tamashiro, Y. and Uechi, S. (2015) Structure of ulvan isolated from the edible green seaweed, Ulva pertusa. Advances in Bioscience and Biotechnology, 6, 645-655.
[14]	Tako, M., R. Ohtoshi, K. Kinjyo and S. Uechi. (2016) The novel pyruvated glucogalactan sulfate isolated from Hypnea pannosa. Advances in Biological Chemistry, 6, 114-125.
[15]	Teruya T., Konishi T., Uechi S., Tamaki, H. and Tako, M. (2007) Anti-proliferative activity of oversulfated fucoidan from commercially cultured Cladisiphon okamuranus TOKIDA in U937 cells. International Journal of Biological Macromolecules. 41, 221-226.
[16]	Teruya T., Tatemoto H., Konishi T., and Tako, M. (2009) Structural characteristics and in vitro macrophage activation of acetyl fucoidan from Cladosiphon okamuranus. Glycoconjugate Journal. 26, 1019-1028.
[17]	Tako, M. and Nakamura, S. (1986) Indicative evidence for a conformational transition in κ-carrageenan from studies of viscosity-shear rate dependence. Carbohydrate Research, 155, 200–205.
[18]	Tako, M. and Nakamura, S. (1986) Synergistic interaction between kappa-carrageenan and locust-bean gum in aqueous media. Agricultural Biological chemistry, 50, 2817-2822.
[19]	Qi, Z.-Q., Tako, M. and Toyama, S. (1997) Molecular origin of the rheological characteristics of κ-carrageenan isolated from Ibaranori (Hypnea charoides LAMOUROUX). Journal of Applied Glycoscience, 44, 531-536.
[20]	Tako, M., Nakamura, S. and Kohda, Y. (1987) Indicative evidence for a conformational transition in ι-carrageenan. Carbohydrate Research, 161, 247-253.
[21]	Lin, L.-H., Tako, M and Hongo, F. (2001) Molecular origin for rheological characteristics of ι-carrageenan isolated from Eucheuma serra. Food Science and Technology Research, 7, 176-181.
[22]	Tako, M. and Nakamura, S. (1988) Gelation mechanism of agarose. Carbohydrate Research, 180, 277-284.
[23]	Tako, M. and Kohda Y. (1997) Calcium induced association characteristics of alginate. Journal of Applied Glycoscience, 44, 153-159.
[24]	Teruya, T., Tamaki, Y., Konishi, T. and Tako, M. (2010) Rheological characteristics of alginate isolated from commercially cultured Nemacystus decipiens (Itomozuku). Journal of Applied Glycoscience, 57, 7-12.
[25]	Tako, M. (2000) Structural principles of polysaccharide gels. Journal of Applied Glycoscience, 47, 49-53.
[26]	Tako, M. Tamaki, Y., Teruya,T., Takeda, Y. (2014) The principles of starch gelatinization and retrogradation. Food and Nutrition Sciences, 5, 280-291.
[27]	Tako, M. (2015) The principle of polysaccharide gels. Advances in Bioscience and Biotechnology, 6, 22-36.
[28]	Nishino, T., H. Kiyohara, H., Yamada, H. and Nagumo, T. (1991) An anticoagulant fucoidan from the brown seaweed Ecklonia kurome. Phytochemistry, 30, 535-539.
[29]	Nishino, T., Fukuda, A., Nagumo, T., Fujiwara, M. and Kaji, E. (1999) Inhibition of the generation of thrombin and factor Xa by a fucoidan from the brown seaweed Ecklonia kurome. Thrombosis Research, 96, 37-49.
[30]	Mulloy, B., Mourao, P. A. S. and Gray, E. (2000) Structure/function studies of anticoagulant sulphated polysaccharides using NMR. Journal of Biotechnology, 77, 123-135.
[31]	Zhang, H. J., Mao, W. J., Fang, F., Li, H. Y., Sun, H. H., Chen, Y. and Qi, X. H. (2008) Chemical characteristics and anticoagulant activities of a sulfated polysaccharide and its fragments from Monostroma latissimum. Carbohydrate Polymers, 71, 428-434.
[32]	Majdoub, H., Mansour, M. B., Chaubet, F., Roudsli, M.S. and Maaroufi, R. M. (2009) Anticoagulant activity of a sulfated polysaccharide from the green alga Arthrospira platensis. Biochemica et Biophysica acta,, 1790, 1372-1381.
[33]	Xue, N. L., He, H., Wang, S., Cao, S., Xia Z., Xian, H., Qin, L. and Ma, W. (2017) Structure and anticoagulant property of a sulfated polysaccharide isolated from the green seaweed, Monostroma angiicava. Carbohydrate Polymers, 159, 195-206.
[34]	Harada, N. and Maeda, M. C. (1998) Chemical structure of antithrombin-active rhamnan sulfate. Bioscience, Biotechnology, and Biochemistry, 62, 1648-1652.
[35]	Dubois, M., Gilles, K. A., Hamilton, J. K., Reber P. A. and Smith, F. (1956) Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28, 350-356.
[36]	Bitter, T. Muir, H. M. (1962) A modified uronic acid carbazole reaction. Analytical Biochemistry, 4, 330-334.
[37]	Dodgson, K. S. and Price, R. G. (1962) A note on the determination of the ester sulphate content of sulphated polysaccharides. Biochmical Journal, 84, 106-111.
[38]	Anderson, L. O., Barroweliffe, T. W., Holmer, E., Johnson, E. A. and Sims, G. E. C. (1976) Anticoagulant properties of heparin fractionated by affinity chromatography on matrix bound antithrombin III and by gel filtration. Thrombosis Research, 9, 575-583.
[39]	Quick, A. J. (1940) The clinical application of the hyaluronic acid and the prothrombin tests. American Journal of Clinical Pathology, 10, 222-233.
[40]	Denson, K. W. and Bonnar, J. (1973) The measurement of heparin. A method based on the potentiation of antifactor Xa. Thrombosis Diathesis Haemorrhagica, 30, 471-479.

Cite This Article

Plain Text BibTeX RIS

APA Style

Youichi Yamashiro, Masahiro Nakamura, Takuya Yogi, Takeshi Teruya, Teruko Konishi, et al. (2017). Anticoagulant Activity of Rhamnan Sulfate Isolated from Commercially Cultured Monostroma nitidum. International Journal of Biomedical Materials Research, 5(3), 37-43. https://doi.org/10.11648/j.ijbmr.20170503.12

Copy | Download

ACS Style

Youichi Yamashiro; Masahiro Nakamura; Takuya Yogi; Takeshi Teruya; Teruko Konishi, et al. Anticoagulant Activity of Rhamnan Sulfate Isolated from Commercially Cultured Monostroma nitidum. Int. J. Biomed. Mater. Res. 2017, 5(3), 37-43. doi: 10.11648/j.ijbmr.20170503.12

Copy | Download

AMA Style

Youichi Yamashiro, Masahiro Nakamura, Takuya Yogi, Takeshi Teruya, Teruko Konishi, et al. Anticoagulant Activity of Rhamnan Sulfate Isolated from Commercially Cultured Monostroma nitidum. Int J Biomed Mater Res. 2017;5(3):37-43. doi: 10.11648/j.ijbmr.20170503.12

Copy | Download

@article{10.11648/j.ijbmr.20170503.12,
  author = {Youichi Yamashiro and Masahiro Nakamura and Takuya Yogi and Takeshi Teruya and Teruko Konishi and Shuntoku Uechi and Masakuni Tako},
  title = {Anticoagulant Activity of Rhamnan Sulfate Isolated from Commercially Cultured Monostroma nitidum},
  journal = {International Journal of Biomedical Materials Research},
  volume = {5},
  number = {3},
  pages = {37-43},
  doi = {10.11648/j.ijbmr.20170503.12},
  url = {https://doi.org/10.11648/j.ijbmr.20170503.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbmr.20170503.12},
  abstract = {The green seaweed, Monostroma nitidum, is widespread in Japan. In Okinawa Prefecture, the production of seaweed is performed using culture-nets that are seeded artificially. Algae contain a soluble polysaccharide, rhamnan sulfate. To estimate its applicability, the anticoagulant activity of rhamnan sulfate was investigated. Rhamanan sulfate was fractionated by ion-exchange chromatography on a DEAE-sepharose column, and two fractions (A and B) were obtained. Partially hydrolyzed rhamnan sulfates with different molecular mass (C1, C2 and C3) were also prepared. The activated partial thromboplastin time (APTT) test, prothrombin time (PT) and thrombin time (TT) were applied using human plasma and compared with standard heparin (174 units/mg). The native rhamnan sulfate (molecular mass, 630 kDa; sulfuric acid content, 22.7%), fraction A (12.4%) and fraction B (27.8%) showed approximately 73% APTT activity in comparison with that of standard heparin, but fraction C2 (molecular mass, 450 kDa) had a higher activity than that of the standard (107%). On the other hand, in the PT assay, all fractions except fraction C2 and C3 (370 kDa) showed higher activity approximately 120-155% greater than that of standard heparin. The TT activity of rhamnan sulfate depended on the sulfate content, and that of fraction B, which has high sulfuric acid content (27.8%), was 135-173% greater than that of heparin. The sulfate groups of L-rhamnosyl residues and carboxyl group of D-glucuronosyl residue on the trisaccharide side chains of the rhamnan sulfate might interact strongly with the active site of thrombin molecules. The results and discussion suggested that rhamnan sulfate from commercially cultured Monostroma nitidum could be a potential anticoagulant polysaccharide.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Anticoagulant Activity of Rhamnan Sulfate Isolated from Commercially Cultured Monostroma nitidum
AU  - Youichi Yamashiro
AU  - Masahiro Nakamura
AU  - Takuya Yogi
AU  - Takeshi Teruya
AU  - Teruko Konishi
AU  - Shuntoku Uechi
AU  - Masakuni Tako
Y1  - 2017/06/21
PY  - 2017
N1  - https://doi.org/10.11648/j.ijbmr.20170503.12
DO  - 10.11648/j.ijbmr.20170503.12
T2  - International Journal of Biomedical Materials Research
JF  - International Journal of Biomedical Materials Research
JO  - International Journal of Biomedical Materials Research
SP  - 37
EP  - 43
PB  - Science Publishing Group
SN  - 2330-7579
UR  - https://doi.org/10.11648/j.ijbmr.20170503.12
AB  - The green seaweed, Monostroma nitidum, is widespread in Japan. In Okinawa Prefecture, the production of seaweed is performed using culture-nets that are seeded artificially. Algae contain a soluble polysaccharide, rhamnan sulfate. To estimate its applicability, the anticoagulant activity of rhamnan sulfate was investigated. Rhamanan sulfate was fractionated by ion-exchange chromatography on a DEAE-sepharose column, and two fractions (A and B) were obtained. Partially hydrolyzed rhamnan sulfates with different molecular mass (C1, C2 and C3) were also prepared. The activated partial thromboplastin time (APTT) test, prothrombin time (PT) and thrombin time (TT) were applied using human plasma and compared with standard heparin (174 units/mg). The native rhamnan sulfate (molecular mass, 630 kDa; sulfuric acid content, 22.7%), fraction A (12.4%) and fraction B (27.8%) showed approximately 73% APTT activity in comparison with that of standard heparin, but fraction C2 (molecular mass, 450 kDa) had a higher activity than that of the standard (107%). On the other hand, in the PT assay, all fractions except fraction C2 and C3 (370 kDa) showed higher activity approximately 120-155% greater than that of standard heparin. The TT activity of rhamnan sulfate depended on the sulfate content, and that of fraction B, which has high sulfuric acid content (27.8%), was 135-173% greater than that of heparin. The sulfate groups of L-rhamnosyl residues and carboxyl group of D-glucuronosyl residue on the trisaccharide side chains of the rhamnan sulfate might interact strongly with the active site of thrombin molecules. The results and discussion suggested that rhamnan sulfate from commercially cultured Monostroma nitidum could be a potential anticoagulant polysaccharide.
VL  - 5
IS  - 3
ER  -

Copy | Download

Author Information

Youichi Yamashiro

Department of Subtropical Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Nishihara, Okinawa, Japan; Quality Control and Development, Miyahira Milk Co., Ltd, Itoman, Okinawa, Japan
Masahiro Nakamura

Applied Life Science, United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima, Kagoshima, Japan
Takuya Yogi

Department of Subtropical Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Nishihara, Okinawa, Japan
Takeshi Teruya

Department of Subtropical Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Nishihara, Okinawa, Japan
Teruko Konishi

Department of Subtropical Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Nishihara, Okinawa, Japan
Shuntoku Uechi

Department of Subtropical Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Nishihara, Okinawa, Japan
Masakuni Tako

Department of Subtropical Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Nishihara, Okinawa, Japan; Health and Longevity Research Laboratory, Okinawa, Okinawa, Japan

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Youichi Yamashiro, Masahiro Nakamura, Takuya Yogi, Takeshi Teruya, Teruko Konishi, et al. (2017). Anticoagulant Activity of Rhamnan Sulfate Isolated from Commercially Cultured Monostroma nitidum. International Journal of Biomedical Materials Research, 5(3), 37-43. https://doi.org/10.11648/j.ijbmr.20170503.12

Copy | Download

ACS Style

Youichi Yamashiro; Masahiro Nakamura; Takuya Yogi; Takeshi Teruya; Teruko Konishi, et al. Anticoagulant Activity of Rhamnan Sulfate Isolated from Commercially Cultured Monostroma nitidum. Int. J. Biomed. Mater. Res. 2017, 5(3), 37-43. doi: 10.11648/j.ijbmr.20170503.12

Copy | Download

AMA Style

Youichi Yamashiro, Masahiro Nakamura, Takuya Yogi, Takeshi Teruya, Teruko Konishi, et al. Anticoagulant Activity of Rhamnan Sulfate Isolated from Commercially Cultured Monostroma nitidum. Int J Biomed Mater Res. 2017;5(3):37-43. doi: 10.11648/j.ijbmr.20170503.12

Copy | Download

@article{10.11648/j.ijbmr.20170503.12,
  author = {Youichi Yamashiro and Masahiro Nakamura and Takuya Yogi and Takeshi Teruya and Teruko Konishi and Shuntoku Uechi and Masakuni Tako},
  title = {Anticoagulant Activity of Rhamnan Sulfate Isolated from Commercially Cultured Monostroma nitidum},
  journal = {International Journal of Biomedical Materials Research},
  volume = {5},
  number = {3},
  pages = {37-43},
  doi = {10.11648/j.ijbmr.20170503.12},
  url = {https://doi.org/10.11648/j.ijbmr.20170503.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbmr.20170503.12},
  abstract = {The green seaweed, Monostroma nitidum, is widespread in Japan. In Okinawa Prefecture, the production of seaweed is performed using culture-nets that are seeded artificially. Algae contain a soluble polysaccharide, rhamnan sulfate. To estimate its applicability, the anticoagulant activity of rhamnan sulfate was investigated. Rhamanan sulfate was fractionated by ion-exchange chromatography on a DEAE-sepharose column, and two fractions (A and B) were obtained. Partially hydrolyzed rhamnan sulfates with different molecular mass (C1, C2 and C3) were also prepared. The activated partial thromboplastin time (APTT) test, prothrombin time (PT) and thrombin time (TT) were applied using human plasma and compared with standard heparin (174 units/mg). The native rhamnan sulfate (molecular mass, 630 kDa; sulfuric acid content, 22.7%), fraction A (12.4%) and fraction B (27.8%) showed approximately 73% APTT activity in comparison with that of standard heparin, but fraction C2 (molecular mass, 450 kDa) had a higher activity than that of the standard (107%). On the other hand, in the PT assay, all fractions except fraction C2 and C3 (370 kDa) showed higher activity approximately 120-155% greater than that of standard heparin. The TT activity of rhamnan sulfate depended on the sulfate content, and that of fraction B, which has high sulfuric acid content (27.8%), was 135-173% greater than that of heparin. The sulfate groups of L-rhamnosyl residues and carboxyl group of D-glucuronosyl residue on the trisaccharide side chains of the rhamnan sulfate might interact strongly with the active site of thrombin molecules. The results and discussion suggested that rhamnan sulfate from commercially cultured Monostroma nitidum could be a potential anticoagulant polysaccharide.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Anticoagulant Activity of Rhamnan Sulfate Isolated from Commercially Cultured Monostroma nitidum
AU  - Youichi Yamashiro
AU  - Masahiro Nakamura
AU  - Takuya Yogi
AU  - Takeshi Teruya
AU  - Teruko Konishi
AU  - Shuntoku Uechi
AU  - Masakuni Tako
Y1  - 2017/06/21
PY  - 2017
N1  - https://doi.org/10.11648/j.ijbmr.20170503.12
DO  - 10.11648/j.ijbmr.20170503.12
T2  - International Journal of Biomedical Materials Research
JF  - International Journal of Biomedical Materials Research
JO  - International Journal of Biomedical Materials Research
SP  - 37
EP  - 43
PB  - Science Publishing Group
SN  - 2330-7579
UR  - https://doi.org/10.11648/j.ijbmr.20170503.12
AB  - The green seaweed, Monostroma nitidum, is widespread in Japan. In Okinawa Prefecture, the production of seaweed is performed using culture-nets that are seeded artificially. Algae contain a soluble polysaccharide, rhamnan sulfate. To estimate its applicability, the anticoagulant activity of rhamnan sulfate was investigated. Rhamanan sulfate was fractionated by ion-exchange chromatography on a DEAE-sepharose column, and two fractions (A and B) were obtained. Partially hydrolyzed rhamnan sulfates with different molecular mass (C1, C2 and C3) were also prepared. The activated partial thromboplastin time (APTT) test, prothrombin time (PT) and thrombin time (TT) were applied using human plasma and compared with standard heparin (174 units/mg). The native rhamnan sulfate (molecular mass, 630 kDa; sulfuric acid content, 22.7%), fraction A (12.4%) and fraction B (27.8%) showed approximately 73% APTT activity in comparison with that of standard heparin, but fraction C2 (molecular mass, 450 kDa) had a higher activity than that of the standard (107%). On the other hand, in the PT assay, all fractions except fraction C2 and C3 (370 kDa) showed higher activity approximately 120-155% greater than that of standard heparin. The TT activity of rhamnan sulfate depended on the sulfate content, and that of fraction B, which has high sulfuric acid content (27.8%), was 135-173% greater than that of heparin. The sulfate groups of L-rhamnosyl residues and carboxyl group of D-glucuronosyl residue on the trisaccharide side chains of the rhamnan sulfate might interact strongly with the active site of thrombin molecules. The results and discussion suggested that rhamnan sulfate from commercially cultured Monostroma nitidum could be a potential anticoagulant polysaccharide.
VL  - 5
IS  - 3
ER  -

Copy | Download