Management of Faba Bean Rust by Some Antagonistic Bioagents and Induced Resistance Chemicals
International Journal of Biomedical Materials Research
Volume 7, Issue 1, June 2019, Pages: 51-60
Received: Feb. 15, 2019; Accepted: Mar. 20, 2019; Published: Apr. 29, 2019
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Authors
Khairy Abdel-Maksoud Abada, Plant Patholology Department, Faculty of Agriculture, Cairo University, Cairo, Egypt
Amany Mohamed Farouk Attia, Plant Patholology Department, Faculty of Agriculture, Cairo University, Cairo, Egypt
Asmaa Mahmoud Abd-Almoaty Alkolaly, Integrated Control Research Department, Plant Research Institute, Agriculture Research Centre, Giza, Egypt
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Abstract
Eight bacterial and fungal isolates as well as four induced resistance chemicals (IRCs) were assessed in vitro and in vivo against Uromyces viciaefabae (Pers.) Schroet, the causal of faba bean rust. The inhibitory effect of Bacillus spp. was ranged between 35.2-50.4%, Trichoderma spp. between 39.0-59.0%. and IRCs between 34.3- 57.7%. In addition, the highest inhibitory effect on the germinated urediospores of the causal fungus was obtained by B. subtilis, T. viride and bion (BTH). Under greenhouse conditions, spraying faba bean plants with any of the tested bioagents significantly reduced disease severity and increased the produced pod yield compared with the control treatment. Also, spraying plants grown from soaked seeds for 12 hrs before sowing in any of the IRCs or soaking in water only with any of these inducers significantly reduced disease severity and increased the produced pod yield compared with the control treatment. Field experiments during two successive seasons under the natural infection by faba bean rust at Behera governorate revealed that spraying B. subtilis and T. viride, each alone or in combination, on plants grown from soaked seeds in BTH at beginning of the incidence of the disease significantly reduced disease severity and increased the produced seed yield compared with the control. On the other hand, plants sprayed with the mixture of both bioagents were of the lowest infection and produced the highest dry seeds/plot compared with the other treatments and the control. However, the fungicide Topas was the superior treatment for lowering disease severity and increasing the produced seeds yield. Considerable increase in the activity of three oxidative- reductive enzymes, i.e phenyl alanine ammonia lyase (PAL), peroxidase (PO) and polyphenoloxidase (PPO) in the leaves of all treatments compared with both control treatments. Furthermore, plants grown from seeds soaked in BTH showed the highest activity of the three enzymes followed by those sprayed with B.subtilis then with T.viride.
Keywords
Faba-Bean, Bioagents, Disease Management, Induced Resistance Chemicals, Oxidative-Reductive Enzymes, Uromyces Viciae-Fabae, Topas
To cite this article
Khairy Abdel-Maksoud Abada, Amany Mohamed Farouk Attia, Asmaa Mahmoud Abd-Almoaty Alkolaly, Management of Faba Bean Rust by Some Antagonistic Bioagents and Induced Resistance Chemicals, International Journal of Biomedical Materials Research. Vol. 7, No. 1, 2019, pp. 51-60. doi: 10.11648/j.ijbmr.20190701.17
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Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
Abada, K. A.; Mostafa, M. A; Hewidy, M. A. and Sarhan, E. A. D. (2003). Completing the life cycle of Uromyces vicia fabae on faba-bean plants in Egypt. 10th. Cong. of Phytopathol., Giza, Egypt,9-10 December, 53-62.
[2]
Abada, K. A. and Eid, Kh. E. (2013). A Protocol suggested for management of cantaloupe downy mildew. J. of App. Sci. Res., 9(11): 5633-5642.
[3]
Abada, K. A. and Attia, Amany M. F. (2017). Potentiality of induce resistante chemicals and bioagents in managing lettuce downy mildew. Amer. J. of BioSci., 5(1): 4-12.
[4]
Abada, K. A.; Attia, Amany M. F. and Youssef, Maryan M. (2018). Role of chemicals for plant resistance, Trichoderma bioagents and cow whey milk in combination on management of tomato powdery mildew. J. of Biotechnol. and Bioeng., 2(2):1-11.
[5]
Abd-El-Khair, H. R.; Khalifa, K. M.; Hagg, Karima H. E. (2010). Effect of Trichoderma spp. on damping off diseases incidence, some plant enzymes activity and nutritional status of bean plants. J. of Amer. Sci., 6 (9):486-497.
[6]
Barakat, F. M.; Abada, K. A.; Abou-Zeid, N. M. and El-Gammal, Y. H. E.(2014). Effect of volatile and non-volatile compounds of Trichoderma spp. on Botrytis fabae the causative agent of Faba-bean chocolate spot. Amer. J. Life Scis., 2(6-2):11-18.
[7]
Barilli, E.; Diego, R.; Carmine, A.; Antonio, E. and Prats, Elena. (2015). BTH and BABA induce resistance in pea against rust (Uromyces pisi) involving differential phytoalexin accumulation. Planta, 242 (5): 1095-1106. doi: 10.1007/s 00425- 015-2339-8.
[8]
Bissett, J. (1991). A revision of the genus Trichoderma. W: Infragenic classification. Can. J. Bot., 69:2317-2357.
[9]
Bouhassan, A.; Sadiki, M. and Tivoli, B. (2004). Evaluation of a collection of faba bean (Vicia fabae L.) genotypes originating from the Maghreb for resistance to chocolate spot (Botrytis fabae) by assessment in the field and laboratory. Euphytica, 135:55-62.
[10]
Brain, P. W. and Hemming, H. G. (1945). Gliotoxin a fungistatic metabolic product of Trichoderma viride. Ann. Appl. Biol., 32: 214– 220.
[11]
Burrell, M. M. and Rees, T. A. (1974). Metabolism of phenylalanine and tyrosine in rice leaves infected by Pyricularia oryzae. Physiol. Plant Pathol., 4: 497–508.
[12]
Chet, I.; Inbar, J. and Hadar, I. (1997). Fungal antagonists and mycoparasites. In: The Mycota IV: Environmental and Microbial Relationships. Wicklow D. T. and Soderstorm B., eds. pp 165-184.
[13]
Dean, R. A. and Kuc, J. (1985). Induced systemic protection in plants. Trends Biotechnol., 3: 125-128.
[14]
Deshmukh, A. J.; Mehta,. B. P. and Patil, V. A. (2010). In vitro evaluation of some known bioagents to control C. gloeosporioides Penz, and Sacc, causing anthracnose of Indian bean. Int. J. Pharma and Bio. Sci., 1 (2): 1-6
[15]
Dubos, B. and Bulit, J. (1981). Filamentous fungi as biocontrol agents on aerial plant surfaces Ill:. BlackmanJ. P. (ed). Microbial Ecology of the phylloplane. Academic Press, London, pp 353-356.
[16]
Doubrava, N.; Dean R. and Kuc, J. (1988). Induction of systemic resistance to anthracnose caused by Colltetrichum lagenarum from spinach and hubar leaves. Physiol. Mol. Plant Pathol., 33: 60-70.
[17]
Ellis, R. J.; Timms-Wilson, T. M.; Beringer, J. E.; Rhodes, D.; Renwick, A.; Stevenson, L. and Bailey, M. J. (1999). Ecological basis for biocontrol of damping-off disease by Pseudomonas fluorescens 54/96. J. App. Microbiol., 87:454–463.
[18]
El-Mogy, Nehal S. and AbdelKader, M. M. (2018). Bio- and fungicide alternatives treatments for suppressing faba bean rust disease under natural field conditions. Biosci. Res., 15(2):1415-1423.
[19]
Fahim, M. M.; Attia, M. F.; Okasha. A. K. and Abada, K. A. (1987). Trichoderma as a biocontrol agent against root and crown-rots of strawberry. Egypt. J. Phytopathol., 21 (2): 139-148.
[20]
Farkas, L. and Kiraly, L. (1967). Role of phenolic compounds in the physiology of plant disease and disease resistance. Phytopathol. Z., 40: 106-150
[21]
Fisher, R. A. (1948). Statistical Methods for research workers (10th ed.). Oliver 8: Boyd, Edinburgh.
[22]
Fu, J. and Huang, B. (2001). Involvement of antioxidants and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress. Environ Exp. Bot., 45(2):105–114.
[23]
Godoy, C. V.; Carneiro, S. M. T. B. G.; Iamauti, T.; Amorim, L.; Berger, R. D. and Bergamin, F. A. (1997). 4: 336–45.
[24]
Bergey, D. H.; Holt, J. G. and Krieg, N. R. (1984). Bergey’s Manual of Systematic Bacteriology. Baltimore, MD: Williams & Wilkins, ©1984-©1989 Diagrammatic scales for bean diseases: development and validat-ion. Zeitschriftfür Pflanzenkrankheiten und Pflanzens-chutz, 0riology. Williams & Wilkins, Baltimore, USA.
[25]
Iriti, M. and Faoro, F. (2003). Benzothiadiazole (BTH): Induces cell-death independent resistance in Phaseolus vulgaris against Uromyces appendiculatus. J. of Phytopathol., 151(3): 171-180.
[26]
Jacobsen, B. J.; Zidack, N. K. and Larson, B. J. (2004). The role of Bacillus-based biological control agents in integrated pest management systems: Plant Diseases. Phytopathology, 94(11):1272-1275.
[27]
Kessmann, H.; Sataub, T.; Hofmann, C.; Meatzke, T. and Herzog, J. (1994). Induction of systemic acquired disease resistance in plants by chemicals. Ann. Rev. Phytopathol., 32: 439-459.
[28]
Kloepper, J. W.; Ryu, C. M. and Zhang, S. (2004). Induced systemic resistance and promotion of plant growth by Bacillus spp. Phytopathology, 94(11):1259–1266.
[29]
Kosuge, T. (1996). The role of phenolics in host response to infection to infection. Ann. Rev. Phyto-pathol., 7:195–222.
[30]
Kumar, R. N and Mukerji, K. G. (1996). Integrated disease management future perspectives, pp. 335-347. In: K. G. Mukerji, B. Mathur, B. P. Chamala and C. Chitralekha (Eds.), Advances in Botany. APH Publishing Corporation, New Delhi.
[31]
Larcke, P. (1981). Alternative chemical agents for controlling plant diseases. Phil. Trans. Res. Soc., 2: 83- 101.
[32]
Mayer, A. M.; Harel, E. and Shaul, R. B. (1965). Assay of catechol oxidase a critical comparison of methods. Phytochemistry, 5:783–789.
[33]
Melo, G. A.; Shimizu, M. M. and Mazzafera, P. (2006). Polyphenoloxidase activity in coffee leaves and its role in resistance against the coffee leaf miner and coffee leaf rust. Phytochemistry, 67: 277-285.
[34]
Metranx, J. D. and Boller, T.(1986). Local and systemic induction of chitinase in cucumber plants in response to fungal, bacterial and viral infections. Physiol. Mol. Pathol., 28: 161-169.
[35]
Morkunas, I. and Gemerek J. (2007). The possible involvement of peroxidase in defense of yellow lupine embryo axes against Fusarium oxysporum. J. Plant Physiol., 164: 497-506.
[36]
Musloco, A.; Felicim, M.; Concheri, G. and Nardi, S. (1993). Effect of earthworm humic substances on esterase and peroxidase activity during growth of leaf explants of Nicotiana plumbaginifolia. Biol. and Ferti. of Soils, 15, 127-131.
[37]
Nassib, A. M.; Khalil, S. A., El-Botai,. M. A. and Radi, M. M. (1991). In: Nile Valley Regional program on Cool Season Food Legumes and Cereals. 1990/91. Annual Report, Egypt. Cairo: ARC /ICARDA.
[38]
Oedjijono, M. A. L. and Dragar, C. (1993). Isolation of bacteria antagonistic to a range of plant pathogenic fungi. Soil Biol. Biochem., 25: 247–250.
[39]
Parry, J. M.; Turnbull P. C. B. and Gibson J. R. (1983). A colour atlas of Bacillus species, Wolfe Medical Publications Ltd. 390 -396.
[40]
Prasad, B. N. and Kumar, M. R. (2011). Effect of non-volatile compounds produced by Trichoderma spp. on growth and sclerotial viability of Rhizoctonia solani, incident of sheath blight of rice. Indian J. Funda. App. Life Sci.,1 (2) 37-42.
[41]
Raupach, G. S. and Kloepper, J. W. (1998). Mixtures of plant growth promoting rhizobacteria enhance biological control of multiple cucumber pathogens. Phytopathology, 88:1158–1164.
[42]
Rifai, M. A. (1969). A revision of the genus Trichoderma. Mycological Papers, 116:1-56
[43]
Sahile, S.; Sakhuja, P. K.; Fininsa, C. and Ahmed, S. (2011). Potential antagonistic fungal species from Ethiopia for biological control of chocolate spot disease of faba bean. Afr. Crop Sci. J., 19 (3): 213 - 225.
[44]
Scott-Carig, J. S.; Kerby, K. B.; Stein, B. D. and Somerville, S. C. (1995). Expression of an extracellular peroxidase that is induced in barley (Hordium vulgar) by powdery mildew pathogen (Erysiphe graminis f. sp. hordei). Physiol. Mol. Plant Pathol., 47: 407–418.
[45]
Shoda, M. (2000). Bacterial control of plant diseases. J. Biosci. and Bioeng., 89:515-521.
[46]
Snedecor, G. W. and Cochran, W. G. (1967). Statistical Methods. 6th ed. Iowa State. Univ. Press, Ames, Iowa, USA.
[47]
Stoddard, F. L.; Nicholas, A. H.; Rubiales, D.; Thomas, J. and Villegas-Ferna´ndez, A. M. (2010). Integrated pest management in faba bean. Field Crops Res., 115:308–318
[48]
Vey, A.; Hoagland, R. E. and Butt, T. M. (2001). Toxic metabolites of fungal biocontrol agents. Fungi as Biocontrol Agents: Progress, Problems and Potential. In: Butt T. M, Jackson C and N, eds., CAB International, Bristol. pp. 311-346.
[49]
Yan, Z.; Reddy, M. S. and Kloepper, J. W. (2003). Survival and colonization of rhizobacteria in a tomato transplant system. Can. J. Microbiol., 49(6):383–389.
[50]
Zaher, Effat A.; Abada, K. A. and Zyton-Marwa, A. (2013). Effect of combination between bioagents and solarization on management of crown-and stem-rot of Egyptian clover. Amer. J. of Plant Scis., 1 (3):43 -50.
[51]
Zimand, G.; Elad, Y. and Chet, I.(1996). Effect of Trichoderma harzianum on Botrytis cinerea pathogeni-city. Phytopathology, 86: 11, 12551260.
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