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Mesoporous Silica-Coated β-TCP Granules Prepared Using Alginate and In vitro Evaluation

Received: 9 June 2020     Accepted: 23 June 2020     Published: 13 July 2020
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Abstract

β-Tricalcium phosphate (β-TCP) is widely used, along with bone-derived growth factor, due to its poor osteogenic ability. Mesoporous silica (MPS)-coated β-tricalcium phosphate (β-TCP) granules were prepared to improve protein loading capability, and in vitro evaluations of this material were carried out. β-TCP powder containing 2 wt% Al2O3 and 6 wt% SiO2 was prepared via mechanochemical synthesis. A sodium alginate solution containing β-TCP powder was transferred into a calcium chloride solution, and the obtained spherical beads were heated at 1100 to 1300°C to produce TCP granules. The X-ray diffraction (XRD) profile of the β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 was identical to that of the single β-TCP phase when heated to 1300°C. The compressive strengths of the TCP granules prepared using alginate were remarkably improved compared with those of TCP granules prepared using a pan-type granulator. A silica interlayer was formed on the β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 via magnetron sputtering prior to the coating of the MPS. MPS coatings on β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 was carried out by a dip-coating method after silica interlayer coatings, and the β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 were covered by the MPS particles. A silica interlayer may offer bonding between the β-TCP granules and MPS coating. The Alamar blue assay of the MPS-coated TCP granules exhibited excellent cell viability as well as a high protein-adsorption capacity.

Published in International Journal of Biomedical Materials Research (Volume 8, Issue 1)
DOI 10.11648/j.ijbmr.20200801.12
Page(s) 8-13
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Mesoporous Silica, Coatings, β-TCP, Compressive Strength, Cytocompatibility

References
[1] Jarcho M, Bolen CH, Thomas MB, Bobick J, Kay JF, and Doremus RH (1976). Hydroxylapatite synthesis and characterization in dense polycrystalline form, Jounal of Material Science, 11: 2027-2035.
[2] Akao M, Aoki H, and Kato K (1981). Mechanical properties of sintered hydroxyapatite for prosthetic applications, Jounal of Material Science, 16: 809-812.
[3] Kato K, Irmescu R, Saito T, Yokogawa Y, and Takahashi H (2003). Catalytic Properties of Lipases Immobilized on Various Mesoporous Silicates, Bioscience, Biotechnology, and Biochemistry, 67 (1); 203-206.
[4] Yiu HHP, Wright PA, and Botting NP (2001). Enzyme immobilisation using SBA-15 mesoporous molecular sieves with functionalised surfaces, J. Mol. Catal. B, 15 (1-3): 81-92.
[5] Yokogawa Y, Seelan S, and Zhang Y (2006). Hyperstructured Hydroxyapatite Ceramics as a Carrier for Cell and Protein, Key Engineering Materials, 309-311: 939-942.
[6] Yokogawa Y, Ito S, Yamato Y, and Kishida I (2004). The adsorption of cytochrome C on mesoporous silica coated hydroxyapatite ceremics in PBS solution, Key Engineering Materials, 587; 39-42.
[7] Yokogawa Y, Shishido A, and Hiroyasu K (2007). Silica Interlayer Formation of Mesoporous Silica Coatings on Hydroxyapatite Granules, Key Engineering Materials, 758: 19-23.
[8] Mathew M, Schroeder LW, Dickens B, and Brown WE (1977). The crystal structure of -Ca3(PO4)2, Acta Crystallographica Section B, 33 (5); 1325-33.
[9] Kobayashi S and Murakoshi T (2014). Characterization of mechanical properties of bioactivity of hydroxyapatite / β-tricalcium phosphate composites, Advanced Composite Materials, 23 (2): 163-177.
[10] Toriyama M, Kawamura S, Nagae H, and Ishida K (1987). Effect of MgO Addition on Bending Strength of Sintered β-Tricalcium Phosphate Prepared by Mechanochemical Synthesis, Yogyo Kyokai Shi, 95 (8): 822-824.
[11] Hakamazuka K (2008). β-tricalcium phosphate as bone prosthetic material, Bulletin of Ceramic Sociey of Japan., 43 (11): 987.
[12] Yokogawa Y, Shishido A, and Shigarami Y (2018). RF-Magnetron Sputtered Silica Interlayer on β-TCP Granules for Mesoporous Silica Coating, Key Engineering Materials, 782: 207-11.
[13] Hiramatsu Y, Oka Y, and Kiyama H (1965). Rapid Determination of the Tensile Strength of Rocks with Irregular Test Pieces, Journal of the Mining and Metallurgical Institute of Japan, 81 (932): 1024-1030.
[14] Zhang Y, and Yokogawa Y (2008). Effect of drying conditions during synthesis on the properties of hydroxyapatite powders, Journal of Material Science, Materials in Medicine, 19 (2): 623-628.
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    Atsumasa Shishido, Yudai Shigarami, Erath Beeran Ansar, Hari Krishna Varma, Yoshiyuki Yokogawa. (2020). Mesoporous Silica-Coated β-TCP Granules Prepared Using Alginate and In vitro Evaluation. International Journal of Biomedical Materials Research, 8(1), 8-13. https://doi.org/10.11648/j.ijbmr.20200801.12

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    ACS Style

    Atsumasa Shishido; Yudai Shigarami; Erath Beeran Ansar; Hari Krishna Varma; Yoshiyuki Yokogawa. Mesoporous Silica-Coated β-TCP Granules Prepared Using Alginate and In vitro Evaluation. Int. J. Biomed. Mater. Res. 2020, 8(1), 8-13. doi: 10.11648/j.ijbmr.20200801.12

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    AMA Style

    Atsumasa Shishido, Yudai Shigarami, Erath Beeran Ansar, Hari Krishna Varma, Yoshiyuki Yokogawa. Mesoporous Silica-Coated β-TCP Granules Prepared Using Alginate and In vitro Evaluation. Int J Biomed Mater Res. 2020;8(1):8-13. doi: 10.11648/j.ijbmr.20200801.12

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  • @article{10.11648/j.ijbmr.20200801.12,
      author = {Atsumasa Shishido and Yudai Shigarami and Erath Beeran Ansar and Hari Krishna Varma and Yoshiyuki Yokogawa},
      title = {Mesoporous Silica-Coated β-TCP Granules Prepared Using Alginate and In vitro Evaluation},
      journal = {International Journal of Biomedical Materials Research},
      volume = {8},
      number = {1},
      pages = {8-13},
      doi = {10.11648/j.ijbmr.20200801.12},
      url = {https://doi.org/10.11648/j.ijbmr.20200801.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbmr.20200801.12},
      abstract = {β-Tricalcium phosphate (β-TCP) is widely used, along with bone-derived growth factor, due to its poor osteogenic ability. Mesoporous silica (MPS)-coated β-tricalcium phosphate (β-TCP) granules were prepared to improve protein loading capability, and in vitro evaluations of this material were carried out. β-TCP powder containing 2 wt% Al2O3 and 6 wt% SiO2 was prepared via mechanochemical synthesis. A sodium alginate solution containing β-TCP powder was transferred into a calcium chloride solution, and the obtained spherical beads were heated at 1100 to 1300°C to produce TCP granules. The X-ray diffraction (XRD) profile of the β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 was identical to that of the single β-TCP phase when heated to 1300°C. The compressive strengths of the TCP granules prepared using alginate were remarkably improved compared with those of TCP granules prepared using a pan-type granulator. A silica interlayer was formed on the β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 via magnetron sputtering prior to the coating of the MPS. MPS coatings on β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 was carried out by a dip-coating method after silica interlayer coatings, and the β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 were covered by the MPS particles. A silica interlayer may offer bonding between the β-TCP granules and MPS coating. The Alamar blue assay of the MPS-coated TCP granules exhibited excellent cell viability as well as a high protein-adsorption capacity.},
     year = {2020}
    }
    

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  • TY  - JOUR
    T1  - Mesoporous Silica-Coated β-TCP Granules Prepared Using Alginate and In vitro Evaluation
    AU  - Atsumasa Shishido
    AU  - Yudai Shigarami
    AU  - Erath Beeran Ansar
    AU  - Hari Krishna Varma
    AU  - Yoshiyuki Yokogawa
    Y1  - 2020/07/13
    PY  - 2020
    N1  - https://doi.org/10.11648/j.ijbmr.20200801.12
    DO  - 10.11648/j.ijbmr.20200801.12
    T2  - International Journal of Biomedical Materials Research
    JF  - International Journal of Biomedical Materials Research
    JO  - International Journal of Biomedical Materials Research
    SP  - 8
    EP  - 13
    PB  - Science Publishing Group
    SN  - 2330-7579
    UR  - https://doi.org/10.11648/j.ijbmr.20200801.12
    AB  - β-Tricalcium phosphate (β-TCP) is widely used, along with bone-derived growth factor, due to its poor osteogenic ability. Mesoporous silica (MPS)-coated β-tricalcium phosphate (β-TCP) granules were prepared to improve protein loading capability, and in vitro evaluations of this material were carried out. β-TCP powder containing 2 wt% Al2O3 and 6 wt% SiO2 was prepared via mechanochemical synthesis. A sodium alginate solution containing β-TCP powder was transferred into a calcium chloride solution, and the obtained spherical beads were heated at 1100 to 1300°C to produce TCP granules. The X-ray diffraction (XRD) profile of the β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 was identical to that of the single β-TCP phase when heated to 1300°C. The compressive strengths of the TCP granules prepared using alginate were remarkably improved compared with those of TCP granules prepared using a pan-type granulator. A silica interlayer was formed on the β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 via magnetron sputtering prior to the coating of the MPS. MPS coatings on β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 was carried out by a dip-coating method after silica interlayer coatings, and the β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 were covered by the MPS particles. A silica interlayer may offer bonding between the β-TCP granules and MPS coating. The Alamar blue assay of the MPS-coated TCP granules exhibited excellent cell viability as well as a high protein-adsorption capacity.
    VL  - 8
    IS  - 1
    ER  - 

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Author Information
  • Graduate School of Engineering, Osaka City University, Osaka City, Japan

  • Graduate School of Engineering, Osaka City University, Osaka City, Japan

  • Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India

  • Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India

  • Graduate School of Engineering, Osaka City University, Osaka City, Japan

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