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Mesoporous Silica-Coated β-TCP Granules Prepared Using Alginate and In vitro Evaluation
International Journal of Biomedical Materials Research
Volume 8, Issue 1, June 2020, Pages: 8-13
Received: Jun. 9, 2020; Accepted: Jun. 23, 2020; Published: Jul. 13, 2020
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Authors
Atsumasa Shishido, Graduate School of Engineering, Osaka City University, Osaka City, Japan; Olympus Terumo Biomaterials Co., Nagaizumi-cho, Sunto-gun, Shizuoka, Japan
Yudai Shigarami, Graduate School of Engineering, Osaka City University, Osaka City, Japan
Erath Beeran Ansar, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India
Hari Krishna Varma, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India
Yoshiyuki Yokogawa, Graduate School of Engineering, Osaka City University, Osaka City, Japan
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Abstract
β-Tricalcium phosphate (β-TCP) is widely used, along with bone-derived growth factor, due to its poor osteogenic ability. Mesoporous silica (MPS)-coated β-tricalcium phosphate (β-TCP) granules were prepared to improve protein loading capability, and in vitro evaluations of this material were carried out. β-TCP powder containing 2 wt% Al2O3 and 6 wt% SiO2 was prepared via mechanochemical synthesis. A sodium alginate solution containing β-TCP powder was transferred into a calcium chloride solution, and the obtained spherical beads were heated at 1100 to 1300°C to produce TCP granules. The X-ray diffraction (XRD) profile of the β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 was identical to that of the single β-TCP phase when heated to 1300°C. The compressive strengths of the TCP granules prepared using alginate were remarkably improved compared with those of TCP granules prepared using a pan-type granulator. A silica interlayer was formed on the β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 via magnetron sputtering prior to the coating of the MPS. MPS coatings on β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 was carried out by a dip-coating method after silica interlayer coatings, and the β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 were covered by the MPS particles. A silica interlayer may offer bonding between the β-TCP granules and MPS coating. The Alamar blue assay of the MPS-coated TCP granules exhibited excellent cell viability as well as a high protein-adsorption capacity.
Keywords
Mesoporous Silica, Coatings, β-TCP, Compressive Strength, Cytocompatibility
To cite this article
Atsumasa Shishido, Yudai Shigarami, Erath Beeran Ansar, Hari Krishna Varma, Yoshiyuki Yokogawa, Mesoporous Silica-Coated β-TCP Granules Prepared Using Alginate and In vitro Evaluation, International Journal of Biomedical Materials Research. Vol. 8, No. 1, 2020, pp. 8-13. doi: 10.11648/j.ijbmr.20200801.12
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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