Affinity Binding Macroporous Monolithic Cryogel as a Matrix for Extracorporeal Apheresis Medical Devices

Wuraola Akande; Lyuba Mikhalovska; Stuart James; Sergey Mikhalovsky

doi:doi:10.11648/j.ijbmr.20150305.11

| Peer-Reviewed

Affinity Binding Macroporous Monolithic Cryogel as a Matrix for Extracorporeal Apheresis Medical Devices

Wuraola Akande, Lyuba Mikhalovska, Stuart James, Sergey Mikhalovsky

Published in International Journal of Biomedical Materials Research (Volume 3, Issue 5)

Received: 19 August 2015 Accepted: 30 August 2015 Published: 9 September 2015

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Cytapheresis is an extracorporeal separation technique widely used in medicine for elimination of specific classes of blood cells from circulating blood. It has been shown recently to have clinical efficacy in various disease states, such as leukaemia, autoimmune disorders, rheumatoid arthritis, renal allograft rejection and sickle–cell anaemia. The current study was undertaken to produce an affinity-binding column, based upon a macroporous monolithic cryogel with a structure of interconnected pores, with pore size and low flow resistance potentially suitable for use in cytapheresis. The affinity column was produced from poly (2-hydroxyethyl methacrylate) PHEMA cryogels synthesized by free radical polymerization at -12°C. This study involved assessing haemolytic potential, and functionalisation of polymer matrix with biological ligands. Haemolytic potential of poly (2-hydroxyethyl methacrylate) cryogel was established by measuring free haemoglobin after blood filtration through the column. The anti-human albumin (antibody) was chemically coupled to the epoxy derivatised monolithic cryogels and the binding efficiency of anti-human albumin (antibody) to the cryogel was determined. Our results show that approximately 100% of Red blood cells passed through the column with no evidence of haemolysis found in blood eluted. It was found that ~82% of human serum albumin was retained on the monolithic IgG anti-human albumin cryogel matrix. The obtained results suggest that poly (2-hydroxyethyl methacrylate) monolithic cryogel is a non-haemolytic material (haemocompatible matrix) capable of functionalisation with antibody and thus can be an appropriate matrix for use in extracorporeal apheresis system.

Published in	International Journal of Biomedical Materials Research (Volume 3, Issue 5)
DOI	10.11648/j.ijbmr.20150305.11
Page(s)	56-63
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Macroporous Cryogel, Poly (2-Hydroxyethyl Methacrylate), Anti-Human Albumin Antibody, Affinity Cryogel, Ligand Immobilisation, Monolithic Adsorbent, Cell Separation, Haemocompatibility

References

[1]	Ratner, B.D., et al., Biomaterials Science - An Introduction to Materials in Medicine, ed. n. Edition. 2004: Elsevier.
[2]	Ratner, B.D., et al., Introduction - Biomaterials Science: An Evolving, Multidisciplinary Endeavor, in Biomaterials Science (Third Edition), B.D.R.S.H.J.S.E. Lemons, Editor. 2013, Academic Press. p. xxv-xxxix.
[3]	Ueki, Y., et al., Evaluation of filtration leucocytapheresis for use in the treatment of patients with rheumatoid arthritis. Rheumatology, 2000. 39(2): p. 165-171.
[4]	Hidaka, T., et al., Filtration leukocytapheresis therapy in rheumatoid arthritis: A randomized, double-blind, placebo-controlled trial. Arthritis & Rheumatism, 1999. 42(3): p. 431-437.
[5]	Morihiro, O., et al., Granulocytapheresis in the treatment of patients with rheumatoid arthritis. Journal of Artificial Organs, 1997. 21(9): p. 989-994.
[6]	Sawada, K., et al., Leukocytapheresis therapy, performed with leukocyte removal filter, for inflammatory bowel disease. Journal of Gastroenterology, 1995. 30(3): p. 322-329.
[7]	Rembacken, B.J., et al., Granulocyte apheresis in inflammatory bowel disease: Possible mechanisms of effect. Therapeutic Apheresis and Dialysis, 1998. 2(2): p. 93-96.
[8]	Shimoyama, T., et al., Safety and efficacy of granulocyte and monocyte adsorption apheresis in patients with active ulcerative colitis: A multicenter study. Journal of Clinical Apheresis, 2001. 16(1): p. 1-9.
[9]	Bosch, T., et al., Direct adsorption of low-density lipoprotein and lipoprotein(a) from whole blood: Results of the first clinical long-term multicenter study using DALI apheresis. Journal of Clinical Apheresis, 2002. 17(4): p. 161-169.
[10]	Bosch, T. and T. Wendler, Efficacy and safety of DALI-LDL-apheresis in two patients treated with the angiotensin II-receptor 1 antagonist losartan. Therapeutic Apheresis and Dialysis, 2004. 8(4): p. 269-274.
[11]	Shibata, H., T. Kuriyama, and N. Yamawaki, Cellsorba. Therapeutic Apheresis and Dialysis, 2003. 7(1): p. 44-47.
[12]	Saniabadi, A.R., et al., Adacolumn, an adsorptive carrier based granulocyte and monocyte apheresis device for the treatment of inflammatory and refractory diseases associated with leukocytes. Therapeutic Apheresis and Dialysis, 2003. 7(1): p. 48-59.
[13]	Agishi, T., Birth of the concept and the development of extracorporeal immunomodulation. Therapeutic Apheresis, 2002. 6(4): p. 312-316.
[14]	Archontakis, S., P. Alison, and B. Mahmoud, Low-density lipoprotein-apheresis: an update. The British Journal of Cardiology, 2008. 15(2): p. 83-85.
[15]	Bosch, T., et al., DALI apheresis in hyperlipidemic patients: biocompatibility, efficacy, and selectivity of direct adsorption of lipoproteins from whole blood. Artificial Organs, 2000. 24(2): p. 81-90.
[16]	Tetala, K.K.R. and T.A. van Beek, Bioaffinity chromatography on monolithic supports. Journal of Separation Science, 2010. 33(3): p. 422-438.
[17]	Svec, F. and J.M.J. Frechet, New designs of macroporous polymers and supports: from separation to biocatalysis. Science, 1996. 273(5272): p. 205-211.
[18]	Akande Wuraola, et al., Poly (2-Hydroxyethyl Methacrylate) Macroporous Cryogel for Extracorporeal Medical Devices. International Journal of Biomedical Materials Research, 2015. 3(4): p. 46-55.
[19]	Dainiak, M.B., I.Y. Galaev, and B. Mattiasson, Affinity cryogel monoliths for screening for optimal separation conditions and chromatographic separation of cells. Journal of Chromatography A, 2006. 1123(2): p. 145-150.
[20]	Gun'ko, V.M., I.N. Savina, and S.V. Mikhalovsky, Cryogels: morphological, structural and adsorption characterisation. Adv Colloid Interface Sci, 2013. 187-188: p. 1-46.
[21]	Dainiak, M.B., et al., Detachment of affinity-captured bioparticles by elastic deformation of a macroporous hydrogel. Proceedings of the National Academy of Sciences, 2006. 103(4): p. 849-854.
[22]	Savina, I.N., et al., Porous structure and water state in cross-linked polymer and protein cryo-hydrogels. Soft Matter, 2011. 7(9): p. 4276-4283.
[23]	Onodera, H., et al., Development of a device for selective removal of CD4+ T cells. Therapeutic Apheresis and Dialysis, 2003. 7(3): p. 329-333.
[24]	Kumar, A., et al., Affinity fractionation of lymphocytes using a monolithic cryogel. Journal of Immunological Methods, 2003. 283(1-2): p. 185-194.
[25]	Ingavle, G.C., et al., Affinity binding of antibodies to supermacroporous cryogel adsorbents with immobilized protein A for removal of anthrax toxin protective antigen. Biomaterials, 2015. 50: p. 140-53.
[26]	Kempe, D.S., et al., Stimulation of erythrocyte phosphatidylserine exposure by lead ions. American Journal of Physiology - Cell Physiology, 2005. 288(2): p. C396-C402.
[27]	Streller, U., et al., Design and evaluation of novel blood incubation systems for in vitro hemocompatibility assessment of planar solid surfaces. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2003. 66B(1): p. 379-390.
[28]	Henkelman, S., et al., Standardization of incubation conditions for hemolysis testing of biomaterials. Materials Science and Engineering: C, 2009. 29(5): p. 1650-1654.
[29]	Dillingham, E.O., et al., Biological evaluation of polymers I. Poly(methyl methacylate). Journal of Biomedical Materials Research, 1975. 9(6): p. 569-596.
[30]	Blakney, G. B, and A. Dinwoodie, J., A spectrophotometric scanning technique for the rapid determination of plasma hemoglobin. Journal of Clinical Biochemistry, 1975. 8(2): p. 96-102.
[31]	Smith, P.K., et al., Measurement of protein using bicinchoninic acid. Analytical Biochemistry, 1985. 150(1): p. 76-85.
[32]	Kay, L.A., Essentials of haemostasis and thrombosis, in Essentials of haemostasis and thrombosis. 1988, Churchill Livingstone, Edinburgh. p. 290.
[33]	Luo, Q., et al., High-performance affinity chromatography with immobilization of protein A and L-histidine on molded monolith. Biotechnology and Bioengineering, 2002. 80(5): p. 481-489.
[34]	Kumar, A., et al., Affinity binding of cells to cryogel adsorbents with immobilized specific ligands: effect of ligand coupling and matrix architecture. Journal of Molecular Recognition, 2005. 18(1): p. 84-93.
[35]	Anderson, G.P., et al., Effectiveness of protein A for antibody immobilization for a fiber optic biosensor. Biosensors and Bioelectronics, 1997. 12(4): p. 329-336.
[36]	Arvidsson, P., et al., Direct chromatographic capture of enzyme from crude homogenate using immobilized metal affinity chromatography on a continuous supermacroporous adsorbent. Journal of Chromatography A, 2003. 986(2): p. 275-90.
[37]	Ujam, L.B., et al., Isolation of monocytes from human peripheral blood using immuno-affinity expanded-bed adsorption. Biotechnology and Bioengineering, 2003. 83(5): p. 554-566.
[38]	Lozinsky, V., et al., The potential of polymeric cryogels in bioseparation. Bioseparation, 2001. 10(4): p. 163-188.
[39]	Hajizadeh, S., and B. Mattiasson, Cryogels with Affinity Ligands as Tools in Protein Purification, in Affinity Chromatography, S. Reichelt, Editor. 2015, Springer New York. p. 183-200.

Cite This Article

Plain Text BibTeX RIS

APA Style

Wuraola Akande, Lyuba Mikhalovska, Stuart James, Sergey Mikhalovsky. (2015). Affinity Binding Macroporous Monolithic Cryogel as a Matrix for Extracorporeal Apheresis Medical Devices. International Journal of Biomedical Materials Research, 3(5), 56-63. https://doi.org/10.11648/j.ijbmr.20150305.11

Copy | Download

ACS Style

Wuraola Akande; Lyuba Mikhalovska; Stuart James; Sergey Mikhalovsky. Affinity Binding Macroporous Monolithic Cryogel as a Matrix for Extracorporeal Apheresis Medical Devices. Int. J. Biomed. Mater. Res. 2015, 3(5), 56-63. doi: 10.11648/j.ijbmr.20150305.11

Copy | Download

AMA Style

Wuraola Akande, Lyuba Mikhalovska, Stuart James, Sergey Mikhalovsky. Affinity Binding Macroporous Monolithic Cryogel as a Matrix for Extracorporeal Apheresis Medical Devices. Int J Biomed Mater Res. 2015;3(5):56-63. doi: 10.11648/j.ijbmr.20150305.11

Copy | Download

@article{10.11648/j.ijbmr.20150305.11,
  author = {Wuraola Akande and Lyuba Mikhalovska and Stuart James and Sergey Mikhalovsky},
  title = {Affinity Binding Macroporous Monolithic Cryogel as a Matrix for Extracorporeal Apheresis Medical Devices},
  journal = {International Journal of Biomedical Materials Research},
  volume = {3},
  number = {5},
  pages = {56-63},
  doi = {10.11648/j.ijbmr.20150305.11},
  url = {https://doi.org/10.11648/j.ijbmr.20150305.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbmr.20150305.11},
  abstract = {Cytapheresis is an extracorporeal separation technique widely used in medicine for elimination of specific classes of blood cells from circulating blood. It has been shown recently to have clinical efficacy in various disease states, such as leukaemia, autoimmune disorders, rheumatoid arthritis, renal allograft rejection and sickle–cell anaemia. The current study was undertaken to produce an affinity-binding column, based upon a macroporous monolithic cryogel with a structure of interconnected pores, with pore size and low flow resistance potentially suitable for use in cytapheresis. The affinity column was produced from poly (2-hydroxyethyl methacrylate) PHEMA cryogels synthesized by free radical polymerization at -12°C.  This study involved assessing haemolytic potential, and functionalisation of polymer matrix with biological ligands. Haemolytic potential of poly (2-hydroxyethyl methacrylate) cryogel was established by measuring free haemoglobin after blood filtration through the column. The anti-human albumin (antibody) was chemically coupled to the epoxy derivatised monolithic cryogels and the binding efficiency of anti-human albumin (antibody) to the cryogel was determined. Our results show that approximately 100% of Red blood cells passed through the column with no evidence of haemolysis found in blood eluted.  It was found that ~82% of human serum albumin was retained on the monolithic IgG anti-human albumin cryogel matrix. The obtained results suggest that poly (2-hydroxyethyl methacrylate) monolithic cryogel is a non-haemolytic material (haemocompatible matrix) capable of functionalisation with antibody and thus can be an appropriate matrix for use in extracorporeal apheresis system.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - Affinity Binding Macroporous Monolithic Cryogel as a Matrix for Extracorporeal Apheresis Medical Devices
AU  - Wuraola Akande
AU  - Lyuba Mikhalovska
AU  - Stuart James
AU  - Sergey Mikhalovsky
Y1  - 2015/09/09
PY  - 2015
N1  - https://doi.org/10.11648/j.ijbmr.20150305.11
DO  - 10.11648/j.ijbmr.20150305.11
T2  - International Journal of Biomedical Materials Research
JF  - International Journal of Biomedical Materials Research
JO  - International Journal of Biomedical Materials Research
SP  - 56
EP  - 63
PB  - Science Publishing Group
SN  - 2330-7579
UR  - https://doi.org/10.11648/j.ijbmr.20150305.11
AB  - Cytapheresis is an extracorporeal separation technique widely used in medicine for elimination of specific classes of blood cells from circulating blood. It has been shown recently to have clinical efficacy in various disease states, such as leukaemia, autoimmune disorders, rheumatoid arthritis, renal allograft rejection and sickle–cell anaemia. The current study was undertaken to produce an affinity-binding column, based upon a macroporous monolithic cryogel with a structure of interconnected pores, with pore size and low flow resistance potentially suitable for use in cytapheresis. The affinity column was produced from poly (2-hydroxyethyl methacrylate) PHEMA cryogels synthesized by free radical polymerization at -12°C.  This study involved assessing haemolytic potential, and functionalisation of polymer matrix with biological ligands. Haemolytic potential of poly (2-hydroxyethyl methacrylate) cryogel was established by measuring free haemoglobin after blood filtration through the column. The anti-human albumin (antibody) was chemically coupled to the epoxy derivatised monolithic cryogels and the binding efficiency of anti-human albumin (antibody) to the cryogel was determined. Our results show that approximately 100% of Red blood cells passed through the column with no evidence of haemolysis found in blood eluted.  It was found that ~82% of human serum albumin was retained on the monolithic IgG anti-human albumin cryogel matrix. The obtained results suggest that poly (2-hydroxyethyl methacrylate) monolithic cryogel is a non-haemolytic material (haemocompatible matrix) capable of functionalisation with antibody and thus can be an appropriate matrix for use in extracorporeal apheresis system.
VL  - 3
IS  - 5
ER  -

Copy | Download

Author Information

Wuraola Akande

Biomaterials and Medical Devices Research Group, School of Pharmacy and Biomolecular Sciences, Huxley Building, University of Brighton, Brighton, UK; Department of Clinical Pharmacy and Pharmacy Administration, Faculty of Pharmacy, University of Ibadan, Ibadan, Nigeria
Lyuba Mikhalovska

Biomaterials and Medical Devices Research Group, School of Pharmacy and Biomolecular Sciences, Huxley Building, University of Brighton, Brighton, UK
Stuart James

Biomaterials and Medical Devices Research Group, School of Pharmacy and Biomolecular Sciences, Huxley Building, University of Brighton, Brighton, UK
Sergey Mikhalovsky

Biomaterials and Medical Devices Research Group, School of Pharmacy and Biomolecular Sciences, Huxley Building, University of Brighton, Brighton, UK; School of Engineering, Nazarbayev University, Astana, Kazakhstan

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Wuraola Akande, Lyuba Mikhalovska, Stuart James, Sergey Mikhalovsky. (2015). Affinity Binding Macroporous Monolithic Cryogel as a Matrix for Extracorporeal Apheresis Medical Devices. International Journal of Biomedical Materials Research, 3(5), 56-63. https://doi.org/10.11648/j.ijbmr.20150305.11

Copy | Download

ACS Style

Wuraola Akande; Lyuba Mikhalovska; Stuart James; Sergey Mikhalovsky. Affinity Binding Macroporous Monolithic Cryogel as a Matrix for Extracorporeal Apheresis Medical Devices. Int. J. Biomed. Mater. Res. 2015, 3(5), 56-63. doi: 10.11648/j.ijbmr.20150305.11

Copy | Download

AMA Style

Wuraola Akande, Lyuba Mikhalovska, Stuart James, Sergey Mikhalovsky. Affinity Binding Macroporous Monolithic Cryogel as a Matrix for Extracorporeal Apheresis Medical Devices. Int J Biomed Mater Res. 2015;3(5):56-63. doi: 10.11648/j.ijbmr.20150305.11

Copy | Download

@article{10.11648/j.ijbmr.20150305.11,
  author = {Wuraola Akande and Lyuba Mikhalovska and Stuart James and Sergey Mikhalovsky},
  title = {Affinity Binding Macroporous Monolithic Cryogel as a Matrix for Extracorporeal Apheresis Medical Devices},
  journal = {International Journal of Biomedical Materials Research},
  volume = {3},
  number = {5},
  pages = {56-63},
  doi = {10.11648/j.ijbmr.20150305.11},
  url = {https://doi.org/10.11648/j.ijbmr.20150305.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbmr.20150305.11},
  abstract = {Cytapheresis is an extracorporeal separation technique widely used in medicine for elimination of specific classes of blood cells from circulating blood. It has been shown recently to have clinical efficacy in various disease states, such as leukaemia, autoimmune disorders, rheumatoid arthritis, renal allograft rejection and sickle–cell anaemia. The current study was undertaken to produce an affinity-binding column, based upon a macroporous monolithic cryogel with a structure of interconnected pores, with pore size and low flow resistance potentially suitable for use in cytapheresis. The affinity column was produced from poly (2-hydroxyethyl methacrylate) PHEMA cryogels synthesized by free radical polymerization at -12°C.  This study involved assessing haemolytic potential, and functionalisation of polymer matrix with biological ligands. Haemolytic potential of poly (2-hydroxyethyl methacrylate) cryogel was established by measuring free haemoglobin after blood filtration through the column. The anti-human albumin (antibody) was chemically coupled to the epoxy derivatised monolithic cryogels and the binding efficiency of anti-human albumin (antibody) to the cryogel was determined. Our results show that approximately 100% of Red blood cells passed through the column with no evidence of haemolysis found in blood eluted.  It was found that ~82% of human serum albumin was retained on the monolithic IgG anti-human albumin cryogel matrix. The obtained results suggest that poly (2-hydroxyethyl methacrylate) monolithic cryogel is a non-haemolytic material (haemocompatible matrix) capable of functionalisation with antibody and thus can be an appropriate matrix for use in extracorporeal apheresis system.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - Affinity Binding Macroporous Monolithic Cryogel as a Matrix for Extracorporeal Apheresis Medical Devices
AU  - Wuraola Akande
AU  - Lyuba Mikhalovska
AU  - Stuart James
AU  - Sergey Mikhalovsky
Y1  - 2015/09/09
PY  - 2015
N1  - https://doi.org/10.11648/j.ijbmr.20150305.11
DO  - 10.11648/j.ijbmr.20150305.11
T2  - International Journal of Biomedical Materials Research
JF  - International Journal of Biomedical Materials Research
JO  - International Journal of Biomedical Materials Research
SP  - 56
EP  - 63
PB  - Science Publishing Group
SN  - 2330-7579
UR  - https://doi.org/10.11648/j.ijbmr.20150305.11
AB  - Cytapheresis is an extracorporeal separation technique widely used in medicine for elimination of specific classes of blood cells from circulating blood. It has been shown recently to have clinical efficacy in various disease states, such as leukaemia, autoimmune disorders, rheumatoid arthritis, renal allograft rejection and sickle–cell anaemia. The current study was undertaken to produce an affinity-binding column, based upon a macroporous monolithic cryogel with a structure of interconnected pores, with pore size and low flow resistance potentially suitable for use in cytapheresis. The affinity column was produced from poly (2-hydroxyethyl methacrylate) PHEMA cryogels synthesized by free radical polymerization at -12°C.  This study involved assessing haemolytic potential, and functionalisation of polymer matrix with biological ligands. Haemolytic potential of poly (2-hydroxyethyl methacrylate) cryogel was established by measuring free haemoglobin after blood filtration through the column. The anti-human albumin (antibody) was chemically coupled to the epoxy derivatised monolithic cryogels and the binding efficiency of anti-human albumin (antibody) to the cryogel was determined. Our results show that approximately 100% of Red blood cells passed through the column with no evidence of haemolysis found in blood eluted.  It was found that ~82% of human serum albumin was retained on the monolithic IgG anti-human albumin cryogel matrix. The obtained results suggest that poly (2-hydroxyethyl methacrylate) monolithic cryogel is a non-haemolytic material (haemocompatible matrix) capable of functionalisation with antibody and thus can be an appropriate matrix for use in extracorporeal apheresis system.
VL  - 3
IS  - 5
ER  -

Copy | Download