International Journal of Biomedical Materials Research
Volume 5, Issue 1, February 2017, Pages: 1-4
Received: Oct. 31, 2016;
Accepted: Nov. 15, 2016;
Published: Jan. 13, 2017
Views 3422 Downloads 147
E. Jebarubi, Postgraduate and Research Department of Botany, St. Xavier’s College (Autonomous), Palayamkottai, Tirunelveli, Tamil Nadu, India
S. Christopher Patrick Kiladi, Postgraduate and Research Department of Botany, St. Xavier’s College (Autonomous), Palayamkottai, Tirunelveli, Tamil Nadu, India
T. Leon Stephan Raj, Postgraduate and Research Department of Botany, St. Xavier’s College (Autonomous), Palayamkottai, Tirunelveli, Tamil Nadu, India
Medicinal plants are nature’s gifts and used in different traditional medicinal systems of India. Most of the medicinal plants are obtained from forest without proper management and knowledge of collection. For the authentication of medicinal plants from the substituted materials, a genetic diversity study is necessary. In the present investigation molecular diversity of Cipadessa baccifera was carried out using dominant ISSR (inter simple sequence repeats) markers in four different accessions of Western Ghats of South India. Five primers were selected from a total of ten primers based on the reproducibility of the bands. The number of polymorphic loci was 13 and the percentage of polymorphic loci was 56.52. The genetic distance among the populations ranged from 0.0910 to 0.3629 and the genetic identity ranged from 0.6087 to 0.9130. The overall observed and effective number of alleles was about 1.5652 and 1.3913. Nei’s overall genetic diversity and Shannon information index were 0.2283 and 0.3349. The dendrogram was constructed based on the UPGMA method and the clusters formed depending upon the genetic characters.
S. Christopher Patrick Kiladi,
T. Leon Stephan Raj,
Molecular Diversity of Cipadessa baccifera (Roth) Miq Based on Inter Simple Sequence Repeats, International Journal of Biomedical Materials Research.
Vol. 5, No. 1,
2017, pp. 1-4.
Chetty, K. M, Sivaji, K. and Rao, K. T. Flowering Plants of Chittor District Andhra Pradesh, India. 1st ed. Tirupati. Students Offset Printers, 2008, Pp. 64.
Roy, A. and Shailendra, S. (2006). Limonoids. Overview of significant bioactive triterpenes distributed in plants kingdom. Biological and Pharmaceutical Bulletin,29(2). Pp. 191-201.
Kindo, I, Britto, S. J, Arulappan, M. T, Thomas, S, George, M. and Marandi, R. (2014). Evaluation of the antioxidant activity of ethanolic extracts of three ethnomedicinal plants. Eur J Biomed Pharm Sci, 1(2). Pp. 190-198.
Liang, L, Zhong, C. C. and Xiao, Z. Y. (1991). Study on chemical constituents of Cipadessacinerascens. Zhongcaoyao, 22. Pp. 6-8.
Luo, X. D, Wu, S. H, Ma, Y. B. and Wu, D. G. (2005). Components of Cipadessabaccifera. Phytochemistry, 5(8). Pp. 867-72.
Thirunavukarasu, T, Santhana Lakshmi, K, Tamilarasan, M, Sivamani, S, Sangeetha, D. and Rajash, T. P. (2014). In vitro antimicrobial, antioxidant, haemolytic, thrombolytic activities and phytochemical analysis of Cipadessabaccifera leaves extracts. Int. J. Phytomed, 6(1). Pp. 109-14.
Marshall, D. F. (1997). “Meeting training needs in developing countries,” in Molecular Genetic Techniques for Plant Genetic Resources. Report of an IPGRI Workshop, W. G. Ayad, T. Hodgkin, A. Jaradat, and V. R. Rao, Eds. Pp. 128-134.
Hubby, J. L. and Lewontin, R. C. (1996). A molecular approach to the study of genetic heterozygosity in natural populations. I. The number of alleles at different loci in Drosophila pseudoobscura. Genetics. 54(2). Pp.577-594.
Powell, W, Morgante, M. and Andre, C, Hanafey, M, Vogel, J, Tingey, S, Rafalski, A. (1996). The Comparison of RFLP, RAPD, AFLP and SSR (microsatellite) marker for germplasm analysis. Molecular Breeding, 2(3). Pp. 225-238.
Russell, J. R, Fuller, J. D, Macaulay, M, Hatz, B. G, Jahoor, A, Powell, W. and Waugh, R. (1997). Direct comparison of levels of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs and RAPDs. Theoretical and Applied Genetics, 95. Pp.714-722.
Ammiraju, J. S. S, Dholakia, B. B, Santra, D. K, Singh, H, Lagu, M. D, Tamhankar, S. A, Dhaliwal, H. S, Rao, V. S, Gupta, V. S. and Ranjekar, P. K.(2001). Identification of inter simple sequence repeat (ISSR) markers associated with seed size in wheat. Theoretical and Applied Genetics, 102(5). Pp. 726-732.
Ratnaparkhe, M. B, Tekeoglu, M. and Muehlbauer, F. J. (1998). Inter simple sequence repeat (ISSR) polymorphisms are useful for finding markers associated with disease resistance gene clusters. Theor. Appl. Genet, 97(4). Pp.515-519.
Sica, M, Gamba, G, Gaudio, L. and Aceto, S. (2005). ISSR markers show differentiation among Italian populations of Asparagus acutifolius L. BMC Genetics. 6(17). Pp. 1-7.
Wolfe, A. D, Xiang, Q. Y. and Kephart, S. R. (1998). Diploid hybrid speciation in Penstemon (Scrophulariaceae). Proc. Natl. Acad. Sci. 95(9). Pp. 5112-5115.
Bornet, B, Muller, C, Paulus, F. and Branchard, M. L. (2002). Highly informative nature of inter simple sequence repeat (ISSR) sequences amplified using triand tetra-nucleotide primers from DNA of cauliflower (Brassica oleracea var. botrytis L.). Genome.45. Pp. 890-896.
Bornet, B. and Branchard, M. (2004). Use of ISSR fingerprints to detect microsatellites and genetic diversity in several related Brassica taxa and Arabidopsis thaliana. Hereditas, 140(3). Pp. 245-248.
Archak, S, Gaikwad, A. B, Gautam, D, Rao, E. V, Swamy, K. R. M. and Karihaloo, J. L. (2003). Comparative assessment of DNA fingerprinting techniques (RAPD, ISSR, and AFLP) for genetic analysis of cashew (Anacardiumoccidentale L.) accessions of India. Genome, 46(3). Pp. 362-369.
Doyle, J. J. and Doyle, J. L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bulletin, 19. Pp. 11–15.
Sambrook, J. and Russell, D. W. Molecular Cloning. A Laboratory Manual. New York. Cold Spring Harbor Laboratory Press. 2001.
Williams, J. G. K, Kubelik, A. R, Livak, K. J, Rafalski, and Tin-joy, S. V. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res, 18. Pp. 6531– 6535.
Nei, M, Li, W. H. (1979). Mathematical models for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA. 76. Pp. 5269-5273.
Nei, M. (1973). Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences of the USA.70. Pp. 3321-3323.
Nei, M. (1978). Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics. 89(3). Pp. 583-90.
Langridge, P. and Chalmers, K. J. The principle. Identification and application of molecular markers. In. Lorz H and Wenzel G eds. Biotechnology in Agriculture and Forestry 55. Molecular Marker Systems in Plant Breeding and Crop Improvement, chapter 1.1, pp. 3-22. Springer-Verlag. 2004.
Christopoulos, M. V, Rouskas, D, Tsantili, E. and Bebeli, P. J. (2010). Germplasm diversity and genetic relationships among walnut (Juglans regia L.) cultivars and Greek local selections revealed by Inter-Simple Sequence Repeat (ISSR) markers. Scientia Horticulturae. 125(4). Pp. 584-592.
Jabbarzadeh, Z, Khosh-Khui, M, Salehi, H. and Saberivand A. (2010). Inter simple sequence repeat (ISSR) markers as reproducible and specific tools for genetic diversity analysis of Rose species. African Journal of Biotechnology. 9 (37). Pp.6091-6095.
Su, H, Wang, L, Liu, L, Chi, X, Zhang, Y. (2008). Use of inter-simple sequence repeat markers to develop strain-specific SCAR markers for Flammulina velutipes. J. Appl. Genet. 49(3). Pp. 233-235.