Mesoporous Silica-Coated β-TCP Granules Prepared Using Alginate and <i>In vitro</i> Evaluation

Atsumasa Shishido; Yudai Shigarami; Erath Beeran Ansar; Hari Krishna Varma; Yoshiyuki Yokogawa

doi:doi:10.11648/j.ijbmr.20200801.12

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Mesoporous Silica-Coated β-TCP Granules Prepared Using Alginate and In vitro Evaluation

Atsumasa Shishido, Yudai Shigarami, Erath Beeran Ansar, Hari Krishna Varma, Yoshiyuki Yokogawa

Published in International Journal of Biomedical Materials Research (Volume 8, Issue 1)

Received: 9 June 2020 Accepted: 23 June 2020 Published: 13 July 2020

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Abstract

β-Tricalcium phosphate (β-TCP) is widely used, along with bone-derived growth factor, due to its poor osteogenic ability. Mesoporous silica (MPS)-coated β-tricalcium phosphate (β-TCP) granules were prepared to improve protein loading capability, and in vitro evaluations of this material were carried out. β-TCP powder containing 2 wt% Al₂O₃ and 6 wt% SiO₂ was prepared via mechanochemical synthesis. A sodium alginate solution containing β-TCP powder was transferred into a calcium chloride solution, and the obtained spherical beads were heated at 1100 to 1300°C to produce TCP granules. The X-ray diffraction (XRD) profile of the β-TCP granules containing 2 wt% Al₂O₃ and 6 wt% SiO₂ was identical to that of the single β-TCP phase when heated to 1300°C. The compressive strengths of the TCP granules prepared using alginate were remarkably improved compared with those of TCP granules prepared using a pan-type granulator. A silica interlayer was formed on the β-TCP granules containing 2 wt% Al₂O₃ and 6 wt% SiO₂ via magnetron sputtering prior to the coating of the MPS. MPS coatings on β-TCP granules containing 2 wt% Al₂O₃ and 6 wt% SiO₂ was carried out by a dip-coating method after silica interlayer coatings, and the β-TCP granules containing 2 wt% Al₂O₃ and 6 wt% SiO₂ were covered by the MPS particles. A silica interlayer may offer bonding between the β-TCP granules and MPS coating. The Alamar blue assay of the MPS-coated TCP granules exhibited excellent cell viability as well as a high protein-adsorption capacity.

Published in	International Journal of Biomedical Materials Research (Volume 8, Issue 1)
DOI	10.11648/j.ijbmr.20200801.12
Page(s)	8-13
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Mesoporous Silica, Coatings, β-TCP, Compressive Strength, Cytocompatibility

References

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[3]	Kato K, Irmescu R, Saito T, Yokogawa Y, and Takahashi H (2003). Catalytic Properties of Lipases Immobilized on Various Mesoporous Silicates, Bioscience, Biotechnology, and Biochemistry, 67 (1); 203-206.
[4]	Yiu HHP, Wright PA, and Botting NP (2001). Enzyme immobilisation using SBA-15 mesoporous molecular sieves with functionalised surfaces, J. Mol. Catal. B, 15 (1-3): 81-92.
[5]	Yokogawa Y, Seelan S, and Zhang Y (2006). Hyperstructured Hydroxyapatite Ceramics as a Carrier for Cell and Protein, Key Engineering Materials, 309-311: 939-942.
[6]	Yokogawa Y, Ito S, Yamato Y, and Kishida I (2004). The adsorption of cytochrome C on mesoporous silica coated hydroxyapatite ceremics in PBS solution, Key Engineering Materials, 587; 39-42.
[7]	Yokogawa Y, Shishido A, and Hiroyasu K (2007). Silica Interlayer Formation of Mesoporous Silica Coatings on Hydroxyapatite Granules, Key Engineering Materials, 758: 19-23.
[8]	Mathew M, Schroeder LW, Dickens B, and Brown WE (1977). The crystal structure of -Ca₃(PO4)₂, Acta Crystallographica Section B, 33 (5); 1325-33.
[9]	Kobayashi S and Murakoshi T (2014). Characterization of mechanical properties of bioactivity of hydroxyapatite / β-tricalcium phosphate composites, Advanced Composite Materials, 23 (2): 163-177.
[10]	Toriyama M, Kawamura S, Nagae H, and Ishida K (1987). Effect of MgO Addition on Bending Strength of Sintered β-Tricalcium Phosphate Prepared by Mechanochemical Synthesis, Yogyo Kyokai Shi, 95 (8): 822-824.
[11]	Hakamazuka K (2008). β-tricalcium phosphate as bone prosthetic material, Bulletin of Ceramic Sociey of Japan., 43 (11): 987.
[12]	Yokogawa Y, Shishido A, and Shigarami Y (2018). RF-Magnetron Sputtered Silica Interlayer on β-TCP Granules for Mesoporous Silica Coating, Key Engineering Materials, 782: 207-11.
[13]	Hiramatsu Y, Oka Y, and Kiyama H (1965). Rapid Determination of the Tensile Strength of Rocks with Irregular Test Pieces, Journal of the Mining and Metallurgical Institute of Japan, 81 (932): 1024-1030.
[14]	Zhang Y, and Yokogawa Y (2008). Effect of drying conditions during synthesis on the properties of hydroxyapatite powders, Journal of Material Science, Materials in Medicine, 19 (2): 623-628.

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APA Style

Atsumasa Shishido, Yudai Shigarami, Erath Beeran Ansar, Hari Krishna Varma, Yoshiyuki Yokogawa. (2020). Mesoporous Silica-Coated β-TCP Granules Prepared Using Alginate and In vitro Evaluation. International Journal of Biomedical Materials Research, 8(1), 8-13. https://doi.org/10.11648/j.ijbmr.20200801.12

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ACS Style

Atsumasa Shishido; Yudai Shigarami; Erath Beeran Ansar; Hari Krishna Varma; Yoshiyuki Yokogawa. Mesoporous Silica-Coated β-TCP Granules Prepared Using Alginate and In vitro Evaluation. Int. J. Biomed. Mater. Res. 2020, 8(1), 8-13. doi: 10.11648/j.ijbmr.20200801.12

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AMA Style

Atsumasa Shishido, Yudai Shigarami, Erath Beeran Ansar, Hari Krishna Varma, Yoshiyuki Yokogawa. Mesoporous Silica-Coated β-TCP Granules Prepared Using Alginate and In vitro Evaluation. Int J Biomed Mater Res. 2020;8(1):8-13. doi: 10.11648/j.ijbmr.20200801.12

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@article{10.11648/j.ijbmr.20200801.12,
  author = {Atsumasa Shishido and Yudai Shigarami and Erath Beeran Ansar and Hari Krishna Varma and Yoshiyuki Yokogawa},
  title = {Mesoporous Silica-Coated β-TCP Granules Prepared Using Alginate and In vitro Evaluation},
  journal = {International Journal of Biomedical Materials Research},
  volume = {8},
  number = {1},
  pages = {8-13},
  doi = {10.11648/j.ijbmr.20200801.12},
  url = {https://doi.org/10.11648/j.ijbmr.20200801.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbmr.20200801.12},
  abstract = {β-Tricalcium phosphate (β-TCP) is widely used, along with bone-derived growth factor, due to its poor osteogenic ability. Mesoporous silica (MPS)-coated β-tricalcium phosphate (β-TCP) granules were prepared to improve protein loading capability, and in vitro evaluations of this material were carried out. β-TCP powder containing 2 wt% Al2O3 and 6 wt% SiO2 was prepared via mechanochemical synthesis. A sodium alginate solution containing β-TCP powder was transferred into a calcium chloride solution, and the obtained spherical beads were heated at 1100 to 1300°C to produce TCP granules. The X-ray diffraction (XRD) profile of the β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 was identical to that of the single β-TCP phase when heated to 1300°C. The compressive strengths of the TCP granules prepared using alginate were remarkably improved compared with those of TCP granules prepared using a pan-type granulator. A silica interlayer was formed on the β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 via magnetron sputtering prior to the coating of the MPS. MPS coatings on β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 was carried out by a dip-coating method after silica interlayer coatings, and the β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 were covered by the MPS particles. A silica interlayer may offer bonding between the β-TCP granules and MPS coating. The Alamar blue assay of the MPS-coated TCP granules exhibited excellent cell viability as well as a high protein-adsorption capacity.},
 year = {2020}
}

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TY  - JOUR
T1  - Mesoporous Silica-Coated β-TCP Granules Prepared Using Alginate and In vitro Evaluation
AU  - Atsumasa Shishido
AU  - Yudai Shigarami
AU  - Erath Beeran Ansar
AU  - Hari Krishna Varma
AU  - Yoshiyuki Yokogawa
Y1  - 2020/07/13
PY  - 2020
N1  - https://doi.org/10.11648/j.ijbmr.20200801.12
DO  - 10.11648/j.ijbmr.20200801.12
T2  - International Journal of Biomedical Materials Research
JF  - International Journal of Biomedical Materials Research
JO  - International Journal of Biomedical Materials Research
SP  - 8
EP  - 13
PB  - Science Publishing Group
SN  - 2330-7579
UR  - https://doi.org/10.11648/j.ijbmr.20200801.12
AB  - β-Tricalcium phosphate (β-TCP) is widely used, along with bone-derived growth factor, due to its poor osteogenic ability. Mesoporous silica (MPS)-coated β-tricalcium phosphate (β-TCP) granules were prepared to improve protein loading capability, and in vitro evaluations of this material were carried out. β-TCP powder containing 2 wt% Al2O3 and 6 wt% SiO2 was prepared via mechanochemical synthesis. A sodium alginate solution containing β-TCP powder was transferred into a calcium chloride solution, and the obtained spherical beads were heated at 1100 to 1300°C to produce TCP granules. The X-ray diffraction (XRD) profile of the β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 was identical to that of the single β-TCP phase when heated to 1300°C. The compressive strengths of the TCP granules prepared using alginate were remarkably improved compared with those of TCP granules prepared using a pan-type granulator. A silica interlayer was formed on the β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 via magnetron sputtering prior to the coating of the MPS. MPS coatings on β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 was carried out by a dip-coating method after silica interlayer coatings, and the β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 were covered by the MPS particles. A silica interlayer may offer bonding between the β-TCP granules and MPS coating. The Alamar blue assay of the MPS-coated TCP granules exhibited excellent cell viability as well as a high protein-adsorption capacity.
VL  - 8
IS  - 1
ER  -

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Author Information

Atsumasa Shishido

Graduate School of Engineering, Osaka City University, Osaka City, Japan
Yudai Shigarami

Graduate School of Engineering, Osaka City University, Osaka City, Japan
Erath Beeran Ansar

Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India
Hari Krishna Varma

Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India
Yoshiyuki Yokogawa

Graduate School of Engineering, Osaka City University, Osaka City, Japan

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APA Style

Atsumasa Shishido, Yudai Shigarami, Erath Beeran Ansar, Hari Krishna Varma, Yoshiyuki Yokogawa. (2020). Mesoporous Silica-Coated β-TCP Granules Prepared Using Alginate and In vitro Evaluation. International Journal of Biomedical Materials Research, 8(1), 8-13. https://doi.org/10.11648/j.ijbmr.20200801.12

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ACS Style

Atsumasa Shishido; Yudai Shigarami; Erath Beeran Ansar; Hari Krishna Varma; Yoshiyuki Yokogawa. Mesoporous Silica-Coated β-TCP Granules Prepared Using Alginate and In vitro Evaluation. Int. J. Biomed. Mater. Res. 2020, 8(1), 8-13. doi: 10.11648/j.ijbmr.20200801.12

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AMA Style

Atsumasa Shishido, Yudai Shigarami, Erath Beeran Ansar, Hari Krishna Varma, Yoshiyuki Yokogawa. Mesoporous Silica-Coated β-TCP Granules Prepared Using Alginate and In vitro Evaluation. Int J Biomed Mater Res. 2020;8(1):8-13. doi: 10.11648/j.ijbmr.20200801.12

Copy | Download

@article{10.11648/j.ijbmr.20200801.12,
  author = {Atsumasa Shishido and Yudai Shigarami and Erath Beeran Ansar and Hari Krishna Varma and Yoshiyuki Yokogawa},
  title = {Mesoporous Silica-Coated β-TCP Granules Prepared Using Alginate and In vitro Evaluation},
  journal = {International Journal of Biomedical Materials Research},
  volume = {8},
  number = {1},
  pages = {8-13},
  doi = {10.11648/j.ijbmr.20200801.12},
  url = {https://doi.org/10.11648/j.ijbmr.20200801.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbmr.20200801.12},
  abstract = {β-Tricalcium phosphate (β-TCP) is widely used, along with bone-derived growth factor, due to its poor osteogenic ability. Mesoporous silica (MPS)-coated β-tricalcium phosphate (β-TCP) granules were prepared to improve protein loading capability, and in vitro evaluations of this material were carried out. β-TCP powder containing 2 wt% Al2O3 and 6 wt% SiO2 was prepared via mechanochemical synthesis. A sodium alginate solution containing β-TCP powder was transferred into a calcium chloride solution, and the obtained spherical beads were heated at 1100 to 1300°C to produce TCP granules. The X-ray diffraction (XRD) profile of the β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 was identical to that of the single β-TCP phase when heated to 1300°C. The compressive strengths of the TCP granules prepared using alginate were remarkably improved compared with those of TCP granules prepared using a pan-type granulator. A silica interlayer was formed on the β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 via magnetron sputtering prior to the coating of the MPS. MPS coatings on β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 was carried out by a dip-coating method after silica interlayer coatings, and the β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 were covered by the MPS particles. A silica interlayer may offer bonding between the β-TCP granules and MPS coating. The Alamar blue assay of the MPS-coated TCP granules exhibited excellent cell viability as well as a high protein-adsorption capacity.},
 year = {2020}
}

Copy | Download

TY  - JOUR
T1  - Mesoporous Silica-Coated β-TCP Granules Prepared Using Alginate and In vitro Evaluation
AU  - Atsumasa Shishido
AU  - Yudai Shigarami
AU  - Erath Beeran Ansar
AU  - Hari Krishna Varma
AU  - Yoshiyuki Yokogawa
Y1  - 2020/07/13
PY  - 2020
N1  - https://doi.org/10.11648/j.ijbmr.20200801.12
DO  - 10.11648/j.ijbmr.20200801.12
T2  - International Journal of Biomedical Materials Research
JF  - International Journal of Biomedical Materials Research
JO  - International Journal of Biomedical Materials Research
SP  - 8
EP  - 13
PB  - Science Publishing Group
SN  - 2330-7579
UR  - https://doi.org/10.11648/j.ijbmr.20200801.12
AB  - β-Tricalcium phosphate (β-TCP) is widely used, along with bone-derived growth factor, due to its poor osteogenic ability. Mesoporous silica (MPS)-coated β-tricalcium phosphate (β-TCP) granules were prepared to improve protein loading capability, and in vitro evaluations of this material were carried out. β-TCP powder containing 2 wt% Al2O3 and 6 wt% SiO2 was prepared via mechanochemical synthesis. A sodium alginate solution containing β-TCP powder was transferred into a calcium chloride solution, and the obtained spherical beads were heated at 1100 to 1300°C to produce TCP granules. The X-ray diffraction (XRD) profile of the β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 was identical to that of the single β-TCP phase when heated to 1300°C. The compressive strengths of the TCP granules prepared using alginate were remarkably improved compared with those of TCP granules prepared using a pan-type granulator. A silica interlayer was formed on the β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 via magnetron sputtering prior to the coating of the MPS. MPS coatings on β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 was carried out by a dip-coating method after silica interlayer coatings, and the β-TCP granules containing 2 wt% Al2O3 and 6 wt% SiO2 were covered by the MPS particles. A silica interlayer may offer bonding between the β-TCP granules and MPS coating. The Alamar blue assay of the MPS-coated TCP granules exhibited excellent cell viability as well as a high protein-adsorption capacity.
VL  - 8
IS  - 1
ER  -

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