Investigation of the Process of Biodegradation of Aromatic Hydrocarbons and Phenols by a Method of Reversed-Phase High-Efficient Liquid Chromatography
Science Journal of Analytical Chemistry
Volume 1, Issue 2, November 2013, Pages: 7-11
Received: Sep. 15, 2013; Published: Oct. 20, 2013
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Authors
VELIYEV MAMMAD GUSEINALI, Laboratory “High-unsaturated compounds”, Sumgayit, Azerbaijan; Institute of Polymer Materials of Azerbaijan National Academy of Sciences, Sumgayit, Azerbaijan
Salmanov Mamed Ahad, Institute of Polymer Materials of Azerbaijan National Academy of Sciences, Sumgayit, Azerbaijan; Institute of Microbiology of Azerbaijan National Academy of Sciences, Baku, Azerbaijan
Bektashi Nazim Rauf, Institute of Polymer Materials of Azerbaijan National Academy of Sciences, Sumgayit, Azerbaijan; Institute of Microbiology of Azerbaijan National Academy of Sciences, Baku, Azerbaijan
Aliyeva Saida Rafael, Institute of Polymer Materials of Azerbaijan National Academy of Sciences, Sumgayit, Azerbaijan; Institute of Microbiology of Azerbaijan National Academy of Sciences, Baku, Azerbaijan
Gasanova Arzu Rasul, Institute of Polymer Materials of Azerbaijan National Academy of Sciences, Sumgayit, Azerbaijan; Institute of Microbiology of Azerbaijan National Academy of Sciences, Baku, Azerbaijan
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Abstract
By a method of reversed-phase high-efficient liquid chromatography (HELC) the dynamics of the process of biodegradation of monoaromatic hydrocarbons (benzene, toluene, ethylbenzene) and phenols (phenol, pyrocathechin, hydroquinone, tetrachlorpyrocatechin) by bacteria isolated from coastal waters and soils of Absheron peninsula of the Caspian Sea has been investigated. It has been shown that in the selected conditions, in particular, by bacteria Pseudomonas sp. the symptoms of the products of biotransformation are appeared approximately on sixth day of the exposition. In this case a full formation of the obtained products takes place in 3-4 days and the individual compounds a composition of which remains stable during 25 days are appeared. The used method allowed to detect and to identify in composition of the products of biodegradation more than 15 individual compounds being the products of direct oxidation of aromatic ring and side chain.
Keywords
Biodegradation, Aromatic Hydrocarbons, Liquid Chromatography
To cite this article
VELIYEV MAMMAD GUSEINALI, Salmanov Mamed Ahad, Bektashi Nazim Rauf, Aliyeva Saida Rafael, Gasanova Arzu Rasul, Investigation of the Process of Biodegradation of Aromatic Hydrocarbons and Phenols by a Method of Reversed-Phase High-Efficient Liquid Chromatography, Science Journal of Analytical Chemistry. Vol. 1, No. 2, 2013, pp. 7-11. doi: 10.11648/j.sjac.20130102.11
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