Spectrophotometric Determination of Diazepam in Pharmaceutical Forms by Ion-Pairing with Ferrithiocyanide Complex
Science Journal of Analytical Chemistry
Volume 4, Issue 4, July 2016, Pages: 52-58
Received: Jun. 25, 2016;
Accepted: Jul. 9, 2016;
Published: Jul. 23, 2016
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Ali Abduh Mutair, Department of Chemistry, Faculty of Applied Science, Taiz University, Taiz, Republic of Yemen
Pulikkal Ajmal Koya, Department of Chemistry, National Institute of Technology, Mizoram, India
Niyazi Abdulmawla Sallam Al-Areqi, Department of Chemistry, Faculty of Applied Science, Taiz University, Taiz, Republic of Yemen
A rapid and validated spectrophotometric method has been developed for determination of Diazepam (DZP) in pure and pharmaceutical dosage forms. The method was based on ion-pair association reaction of DZP with ferrithiocyanide complex in acidic medium. The color development was monitored spectrophometrically at the maximum absorption, λmax = 506 nm. The stoichiometry of the ion-pair associate was determined, and the reaction path way was postulated. The proposed method was successfully applied to the determination of DZP in five marketed brands with three labeled dosages; 2, 5, 10 mg per tablet. The analytical results of the proposed spectrophotometric method were statistically compared with those obtained from standard HPLC procedures used as a reference method. Application of the proposed procedure shows acceptable linearity, precision, repeatability, and reproducibility. The analytical results were in good agreement with the label claims. F-and Student’s t-tests proved that no significant difference regarding both accuracy and precision between the proposed and reference method, and that this spectrophotometric method can be employed for the routine analysis of DZP in bulks as well as in the commercial formulations.
Ali Abduh Mutair,
Pulikkal Ajmal Koya,
Niyazi Abdulmawla Sallam Al-Areqi,
Spectrophotometric Determination of Diazepam in Pharmaceutical Forms by Ion-Pairing with Ferrithiocyanide Complex, Science Journal of Analytical Chemistry.
Vol. 4, No. 4,
2016, pp. 52-58.
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