Comparative Chromatographic and Spectrophotometric Methods for Quantitative Estimation of Paracetamol in Analgesic Tablet Dosage Forms
Science Journal of Analytical Chemistry
Volume 5, Issue 6, November 2017, Pages: 98-103
Received: Mar. 10, 2017;
Accepted: Mar. 20, 2017;
Published: Nov. 28, 2017
Views 2626 Downloads 146
Adams Udoji Itodo, Department of Chemistry, College of Sciences, Federal University of Agriculture, Makurdi, Nigeria
Nnodim Vivian Onyinye, Department of Chemistry, College of Sciences, Federal University of Agriculture, Makurdi, Nigeria; Department of Food Safety and Applied Nutrition, National Agency for Food and Drug Administration and Control, Lafia, Nigeria
Poor quality drugs pose a challenge to many countries especially the developing countries. This present study is a scientific approach to further combat medicines counterfeiting as well as the validation of claimed drug specification on labels. This was achieved by comparing two analytical methods viz; spectrophotometric (UV-Vis) and Chromatographic (HPLC) techniques for quantification of Paracetamol (4-acetamidophenol) in selected analgesics marketed in Lafia, Nigeria. The statistical validation parameters such as linearity and methodic error (accuracy, precision) tests were verified and reported in terms of deviation and significance. The tagged 500 mg/tab Paracetamol concentrations for procured Para-P, X, Y and Z were estimated to range between 505.62-570.70 mg/tab and 459.42-512.58 mg/tab using UV-Visible and HPLC respectively. It was reported that experimental paracetamol concentration compares well with the claimed label specification and are within limits prescribed by existing legislation. From results in this study, deviations in API’s concentration values between the two techniques are reasonable but of no statistical significance.
Adams Udoji Itodo,
Nnodim Vivian Onyinye,
Comparative Chromatographic and Spectrophotometric Methods for Quantitative Estimation of Paracetamol in Analgesic Tablet Dosage Forms, Science Journal of Analytical Chemistry.
Vol. 5, No. 6,
2017, pp. 98-103.
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/
) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Akunyili D. N. (2005). Counterfeit and Substandard Drugs, Nigeria‘s Experience: Implications, Challenges, Actions and Recommendations. In Talk for NAFDAC at a Meeting for Key Interest Groups on Health organised by The World Bank.
Arnold L, Collins C, Starmer GA. (1973). The short-term effects of analgesics on the kidney with special reference to acetylsalicylic acid. Pathology. 5 (2): 123-34.
NAFDAC Consumer Safety Bulletin (2006). National Agency for Food and Drug Administration and Control, Abuja, Nigeria: Available at: http://www.nlipw.com/wp-content/uploads/2013/06/EID-GUIDLINESFOR-PACKAGED-WATER.pdf. Accessed 25th Febuary 2014.
Harper, D. (2001). “Online etymology dictionary: analgesia” Retrieved December, 2014.
Hajian R. and Afshari, N. (2012). The spectrophotometric multicomponent Analysis of a ternary mixture of Ibuprofen, Caffeine and Paracetamol by the combination of double divisor-ratio spectra derivative and H-point standard addition method. E-Journal of Chemistry, l 9 (3): 1153-1164.
Haglund B, and Von Bultzing S. I (2006) Combining paracetamol with a selective cyclooxygenase-2 inhibitor for acute pain relief after third molar surgery, a randomized, double- blind, placebo-controlled study. Eur. Journal of Oral Sci. 114, 293-301.
Graham G. G., Scott K. F., day R. O. (2005). Drug safety, 28: 227.
Gazal G, Mackie K, A Comparison of paracetamol Ibuprofen or their combination for pain relief following extrations in children under general anaesthesia randomized controlled trail. Int. J. Paediatr, 17: 167-177.
Mitchell S. J., Alice E. K. and Sarah N. H. (2011). Age-Related Changes in the Hepatic Pharmacology and Toxicology of Paracetamol. Current Gerontology and Geriatrics Research. Article ID 624156, 14 pages. doi: 10.1155/2011/624156.
Sarrell E. M, Wielunsky E, Colon H. A, (2006) Antipyretic treatment in young children with fever, Acetaminophen, Ibuprofen or both alternating in a randomized, double-blind Study. Arch Pediatr, Adolesc Med., 160: 197-202.
Roger B, Aparna M (2011). The Impact of Improved Detection Technology on Drug Quality: A Case Study of Lagos, Nigeria [Online]. Available at: http://www.aei.org/docLib/Nigeria-WorkingPaper-v1.pd. Accessed 2nd March 2015.
Gaudiano M, (2007). Medicines informal market in Congo, Burundi and Angola: counterfeit and sub-standard antimalarials. Malaria Journal.: 6-19.
Ogwal-OkengJW, (2003). Chloroquine in the Ugandan market fails quality test: a pharmacovigilance study. African Health Sciences. 3 (1): 2–6.
Kuldeep D, Ritu K, Prachi K, Sunil K, Pratik P, (2011) “Spectrophotometric Simultaneous Analysis of paracetamol, Propyphenazone and caffeine in Tablet Dosage Forms.” International Journal of Pharmacy. L 3, (3): 170-174.
RSC (2012). Analysis of Paracetamol in tablets by UV Spectrometry. Schools Analyst Competition 2012 Royal Society of Chemistry, Analytical Division. Retrieved 2014 at: http://www.rsc.org/images/North%20East%20Region%202012_tcm18-242912.pdf
Anelise W, Daniele C, Janine M, Karin G and Clarice M (2007) “Validation of UV Spectrophotometric and HPLC Methods for Quantitative Determination of Atenolol in Pharmaceutical Preparations” Latin American Journal of Pharmacy; 26 (5): 765-70.
Devi T. A., Aravind S., Srikanth S, Sivaramaiah N., Smita C. Pawar, J. Venkateshwara R. (2013). Method development and validation of paracetamol drug by RP-HPLC. Journal of Medical & Allied Sciences. 3 (1): 8-14.
Kibwage I O, (1992). The quality work in Daru: Observations during 1983–86. East African Medical Journal, 69: 577–580.
Shakoor O, Taylor RB, Behrens RH. (1997). Assessment of the incidence of substandard drugs in developing countries. Tropical Medicine and International Health.; 2: 839–845.