Optimum Immobilization of <i>Candida Antartica</i> B Lipase on Natural Silica by RSM

Djossou Andriano Jospin; Mazou Mouaïmine; Tchobo Fidèle Paul; Toukourou Akanho Chakirou; Blin Joel; Yao Kouassi Benjamin; Soumanou Mansourou Mohamed

doi:doi:10.11648/j.ajche.20170503.13

Optimum Immobilization of Candida Antartica B Lipase on Natural Silica by RSM

Djossou Andriano Jospin, Mazou Mouaïmine, Tchobo Fidèle Paul, Toukourou Akanho Chakirou, Blin Joel, Yao Kouassi Benjamin, Soumanou Mansourou Mohamed

Published in American Journal of Chemical Engineering (Volume 5, Issue 3)

Received: 22 February 2017 Accepted: 30 March 2017 Published: 23 May 2017

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Abstract

The immobilization of the lipase of Candida antartica B (LCAB) by adsorption on a natural silica support carried out to develop the adsorbent local supports. Immobilization conditions and characterization of the immobilized enzyme were investigated. Response surface methodology (RSM) and 3-level–3-factor fractional factorial design were employed to evaluate the effects of immobilization parameters, such as immobilization time (5-25 hour), immobilization temperature (25-45°C), and enzyme/support ratio (0.1-0.5, w/w), on yield of lipase immobilization on the support. The optimum immobilization conditions were as follows: immobilization time 18 hours, immobilization temperature 20°C, and enzyme / support ratio 0.5 (w/w); with a yield immobilization of 56,13 mg / g. The immobilization lipase shows hydrolytic and synthesis satisfactory activity.

Published in	American Journal of Chemical Engineering (Volume 5, Issue 3)
DOI	10.11648/j.ajche.20170503.13
Page(s)	43-48
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Immobilization, Lipase, Natural Silica Support, RSM

References

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APA Style

Djossou Andriano Jospin, Mazou Mouaïmine, Tchobo Fidèle Paul, Toukourou Akanho Chakirou, Blin Joel, et al. (2017). Optimum Immobilization of Candida Antartica B Lipase on Natural Silica by RSM. American Journal of Chemical Engineering, 5(3), 43-48. https://doi.org/10.11648/j.ajche.20170503.13

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ACS Style

Djossou Andriano Jospin; Mazou Mouaïmine; Tchobo Fidèle Paul; Toukourou Akanho Chakirou; Blin Joel, et al. Optimum Immobilization of Candida Antartica B Lipase on Natural Silica by RSM. Am. J. Chem. Eng. 2017, 5(3), 43-48. doi: 10.11648/j.ajche.20170503.13

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AMA Style

Djossou Andriano Jospin, Mazou Mouaïmine, Tchobo Fidèle Paul, Toukourou Akanho Chakirou, Blin Joel, et al. Optimum Immobilization of Candida Antartica B Lipase on Natural Silica by RSM. Am J Chem Eng. 2017;5(3):43-48. doi: 10.11648/j.ajche.20170503.13

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@article{10.11648/j.ajche.20170503.13,
  author = {Djossou Andriano Jospin and Mazou Mouaïmine and Tchobo Fidèle Paul and Toukourou Akanho Chakirou and Blin Joel and Yao Kouassi Benjamin and Soumanou Mansourou Mohamed},
  title = {Optimum Immobilization of Candida Antartica B Lipase on Natural Silica by RSM},
  journal = {American Journal of Chemical Engineering},
  volume = {5},
  number = {3},
  pages = {43-48},
  doi = {10.11648/j.ajche.20170503.13},
  url = {https://doi.org/10.11648/j.ajche.20170503.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajche.20170503.13},
  abstract = {The immobilization of the lipase of Candida antartica B (LCAB) by adsorption on a natural silica support carried out to develop the adsorbent local supports. Immobilization conditions and characterization of the immobilized enzyme were investigated. Response surface methodology (RSM) and 3-level–3-factor fractional factorial design were employed to evaluate the effects of immobilization parameters, such as immobilization time (5-25 hour), immobilization temperature (25-45°C), and enzyme/support ratio (0.1-0.5, w/w), on yield of lipase immobilization on the support. The optimum immobilization conditions were as follows: immobilization time 18 hours, immobilization temperature 20°C, and enzyme / support ratio 0.5 (w/w); with a yield immobilization of 56,13 mg / g. The immobilization lipase shows hydrolytic and synthesis satisfactory activity.},
 year = {2017}
}

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TY  - JOUR
T1  - Optimum Immobilization of Candida Antartica B Lipase on Natural Silica by RSM
AU  - Djossou Andriano Jospin
AU  - Mazou Mouaïmine
AU  - Tchobo Fidèle Paul
AU  - Toukourou Akanho Chakirou
AU  - Blin Joel
AU  - Yao Kouassi Benjamin
AU  - Soumanou Mansourou Mohamed
Y1  - 2017/05/23
PY  - 2017
N1  - https://doi.org/10.11648/j.ajche.20170503.13
DO  - 10.11648/j.ajche.20170503.13
T2  - American Journal of Chemical Engineering
JF  - American Journal of Chemical Engineering
JO  - American Journal of Chemical Engineering
SP  - 43
EP  - 48
PB  - Science Publishing Group
SN  - 2330-8613
UR  - https://doi.org/10.11648/j.ajche.20170503.13
AB  - The immobilization of the lipase of Candida antartica B (LCAB) by adsorption on a natural silica support carried out to develop the adsorbent local supports. Immobilization conditions and characterization of the immobilized enzyme were investigated. Response surface methodology (RSM) and 3-level–3-factor fractional factorial design were employed to evaluate the effects of immobilization parameters, such as immobilization time (5-25 hour), immobilization temperature (25-45°C), and enzyme/support ratio (0.1-0.5, w/w), on yield of lipase immobilization on the support. The optimum immobilization conditions were as follows: immobilization time 18 hours, immobilization temperature 20°C, and enzyme / support ratio 0.5 (w/w); with a yield immobilization of 56,13 mg / g. The immobilization lipase shows hydrolytic and synthesis satisfactory activity.
VL  - 5
IS  - 3
ER  -

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Author Information

Djossou Andriano Jospin

Unit of Research in Enzymatic and Food Engineering (URGEA), Polytechnic School of Abomey-Calavi (EPAC), Cotonou, Bénin
Mazou Mouaïmine
Tchobo Fidèle Paul

Unit of Research in Enzymatic and Food Engineering (URGEA), Polytechnic School of Abomey-Calavi (EPAC), Cotonou, Bénin
Toukourou Akanho Chakirou

Laboratory of Energy and Applied Mechanics (LEMA), Polytechnic School of Abomey-Calavi (EPAC), Cotonou, Bénin
Blin Joel

Mixed Unit of Research Engineering of Agropolymères and Emergent Technologies, (UMRIATE / CIRAD), Montpellier, France
Yao Kouassi Benjamin

Laboratory of Industrial Processes, of Synthesis, of Environment and New Energies, Yamoussoukro, C?te d’Ivoire
Soumanou Mansourou Mohamed

Unit of Research in Enzymatic and Food Engineering (URGEA), Polytechnic School of Abomey-Calavi (EPAC), Cotonou, Bénin

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APA Style

Djossou Andriano Jospin, Mazou Mouaïmine, Tchobo Fidèle Paul, Toukourou Akanho Chakirou, Blin Joel, et al. (2017). Optimum Immobilization of Candida Antartica B Lipase on Natural Silica by RSM. American Journal of Chemical Engineering, 5(3), 43-48. https://doi.org/10.11648/j.ajche.20170503.13

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ACS Style

Djossou Andriano Jospin; Mazou Mouaïmine; Tchobo Fidèle Paul; Toukourou Akanho Chakirou; Blin Joel, et al. Optimum Immobilization of Candida Antartica B Lipase on Natural Silica by RSM. Am. J. Chem. Eng. 2017, 5(3), 43-48. doi: 10.11648/j.ajche.20170503.13

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AMA Style

Djossou Andriano Jospin, Mazou Mouaïmine, Tchobo Fidèle Paul, Toukourou Akanho Chakirou, Blin Joel, et al. Optimum Immobilization of Candida Antartica B Lipase on Natural Silica by RSM. Am J Chem Eng. 2017;5(3):43-48. doi: 10.11648/j.ajche.20170503.13

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@article{10.11648/j.ajche.20170503.13,
  author = {Djossou Andriano Jospin and Mazou Mouaïmine and Tchobo Fidèle Paul and Toukourou Akanho Chakirou and Blin Joel and Yao Kouassi Benjamin and Soumanou Mansourou Mohamed},
  title = {Optimum Immobilization of Candida Antartica B Lipase on Natural Silica by RSM},
  journal = {American Journal of Chemical Engineering},
  volume = {5},
  number = {3},
  pages = {43-48},
  doi = {10.11648/j.ajche.20170503.13},
  url = {https://doi.org/10.11648/j.ajche.20170503.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajche.20170503.13},
  abstract = {The immobilization of the lipase of Candida antartica B (LCAB) by adsorption on a natural silica support carried out to develop the adsorbent local supports. Immobilization conditions and characterization of the immobilized enzyme were investigated. Response surface methodology (RSM) and 3-level–3-factor fractional factorial design were employed to evaluate the effects of immobilization parameters, such as immobilization time (5-25 hour), immobilization temperature (25-45°C), and enzyme/support ratio (0.1-0.5, w/w), on yield of lipase immobilization on the support. The optimum immobilization conditions were as follows: immobilization time 18 hours, immobilization temperature 20°C, and enzyme / support ratio 0.5 (w/w); with a yield immobilization of 56,13 mg / g. The immobilization lipase shows hydrolytic and synthesis satisfactory activity.},
 year = {2017}
}

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TY  - JOUR
T1  - Optimum Immobilization of Candida Antartica B Lipase on Natural Silica by RSM
AU  - Djossou Andriano Jospin
AU  - Mazou Mouaïmine
AU  - Tchobo Fidèle Paul
AU  - Toukourou Akanho Chakirou
AU  - Blin Joel
AU  - Yao Kouassi Benjamin
AU  - Soumanou Mansourou Mohamed
Y1  - 2017/05/23
PY  - 2017
N1  - https://doi.org/10.11648/j.ajche.20170503.13
DO  - 10.11648/j.ajche.20170503.13
T2  - American Journal of Chemical Engineering
JF  - American Journal of Chemical Engineering
JO  - American Journal of Chemical Engineering
SP  - 43
EP  - 48
PB  - Science Publishing Group
SN  - 2330-8613
UR  - https://doi.org/10.11648/j.ajche.20170503.13
AB  - The immobilization of the lipase of Candida antartica B (LCAB) by adsorption on a natural silica support carried out to develop the adsorbent local supports. Immobilization conditions and characterization of the immobilized enzyme were investigated. Response surface methodology (RSM) and 3-level–3-factor fractional factorial design were employed to evaluate the effects of immobilization parameters, such as immobilization time (5-25 hour), immobilization temperature (25-45°C), and enzyme/support ratio (0.1-0.5, w/w), on yield of lipase immobilization on the support. The optimum immobilization conditions were as follows: immobilization time 18 hours, immobilization temperature 20°C, and enzyme / support ratio 0.5 (w/w); with a yield immobilization of 56,13 mg / g. The immobilization lipase shows hydrolytic and synthesis satisfactory activity.
VL  - 5
IS  - 3
ER  -

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