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Preparation, Characterization and Antifungal Activity Studies of AgNPs Loaded Silk Fibroin Hydrogels
American Journal of Nano Research and Applications
Volume 8, Issue 2, June 2020, Pages: 28-34
Received: May 23, 2020; Accepted: Jun. 10, 2020; Published: Jun. 28, 2020
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Rezaul Haque Ansary, Department of Chemistry, Rajshahi University, Rajshahi, Bangladesh
Tomal Roy, Department of Chemistry, Rajshahi University, Rajshahi, Bangladesh
Ali Asraf, Department of Chemistry, Rajshahi University, Rajshahi, Bangladesh
Sabina Easmin, Department of Chemistry, Rajshahi University, Rajshahi, Bangladesh
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Silk fibroin hydrogels are promising materials for controlled drug delivery device due to their aqueous process ability, biocompatibility, and biodegradability. The research work is aimed to prepare silk fibroin (SF) hydrogels loaded with Ag nanoparticles and to evaluate its antifungal activities. The Silk fibroin hydrogels were formulated at 37°C using 2% (w/v) silk fibroin aqueous solution either by treating 50% (v/v) of ethanol, or 50% (v/v) of propanol, or 50% (v/v) of glycerol, respectively. Above these, the rate of gelation was sufficiently accelerated by addition of glycerol. The silk fibroin hydrogels and prepared silver nanoparticles (AgNPs) were characterized by using Scanning Electron Microscopy (SEM), Thermo Gravimetrical Analysis (TGA). The encapsulation efficiency and release profile of AgNPs were studied by UV-vis spectrometry. The particle size of AgNPs was measured by Malvern Zetasizer Nano and found 93±5 nm. The encapsulation efficiency and morphology of the hydrogels was affected by the formulation conditions. The in vitro release profile showed an initial burst release of AgNPs followed by controlled release for next 20 hours. The antifungal activity of AgNPs loaded SF hydrogels showed a positive response to Aspergillus Niger pathogen. Therefore, silk fibroin hydrogels might be an effective biopolymeric matrix for antifungal applications.
Silk Fibroin, Controlled Release, Hydrogels, Nanoparticles, Drug Delivery
To cite this article
Rezaul Haque Ansary, Tomal Roy, Ali Asraf, Sabina Easmin, Preparation, Characterization and Antifungal Activity Studies of AgNPs Loaded Silk Fibroin Hydrogels, American Journal of Nano Research and Applications. Vol. 8, No. 2, 2020, pp. 28-34. doi: 10.11648/j.nano.20200802.13
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
de Moraes M. A., Albrecht C. R., Ferreira S. M., Beppu M. M. (2015) Formation of silk fibroin hydrogel and evaluation of its drug release profile. Journal of Applied Polymer Science, 132: 15.
Zeng D. M., Pan J. J., Wang Q., Liu X. F., Wang H., Zhang K. Q. (2015) Controlling silk fibroin microspheres via molecular weight distribution. Materials Science Engineering C, 50: 226-233.
Kundu J., Chung Y. I., Kim Y. H., Tae G., Kundu S. C. (2010) Silk fibroin nanoparticles for cellular uptake and control release. International Journal of Pharmaceutics, 388: 242-250.
Ansary R. H., Rahman M. M., Awang M. B., Katas H., Hadi H., Doolaanea A. A. (2016) Preparation, characterization, and in vitro release studies of insulin-loaded double-walled poly (lactide-co-glycolide) microspheres. Drug Delivery and Translational Research, 6 (3): 308-318.
Ansary R. H., Rahman M. M., Awang M. B., Katas H., Hadi H., Mohamed F., Doolaanea A. A., Kamaruzzaman Y. B. (2016) Preparation, characterization and in vitro release study of BSA-loaded double-walled glucose-poly (lactide-co-glycolide) microspheres. Archives of Pharmacal Research, 39 (9): 1242-1256.
Sharma G, Van Der Walle C. F, Kumar M. R. (2013) Antacid co-encapsulated polyester nanoparticles for peroral delivery of insulin: Development, pharmacokinetics, biodistribution and pharmacodynamics. International Journal of Pharmaceutics, 440 (1): 99-110.
Crotts G., Park T. G. (1995) Preparation of porous and nonporous biodegradable polymeric hollow microspheres. Journal of Controlled Release, 35 (2-3): 91-105.
Ansary R. H., Awang M. B., Rahman M. M. (2014) Biodegradable poly (D, L-lactic-co-glycolic acid)-based micro/nanoparticles for sustained release of protein drugs-A review. Tropical Journal of Pharmaceutical Research, 13 (7): 1179-1190.
Panyam J., Dali M. M., Sahoo S. K., Ma W., Chakravarthi S. S., Amidon G. La., Levy R. J., Labhasetwar V. (2003) Polymer degradation and in vitro release of a model protein from poly (D, L-lactide-co-glycolide) nano-and microparticles. Journal of Controlled Release, 92 (1-2): 173-187.
Sinha V. R., Trehan A. (2003) Biodegradable microspheres for protein delivery. Journal of Controlled Release, 90 (3): 261-280.
Lu Z., Meng M., Jiang Y., Xie J. (2014) UV-assisted in situ synthesis of silver nanoparticles on silk fibers for antibacterial applications. Colloids and Surfaces: A, 447: 1–7.
Jiang H. L., Jin J. F., Hu Y. Q., Zhu K. J. (2004) Improvement of protein loading and modulation of protein release from poly (lactide-co-glycolide) microspheres by complexation of proteins with polyanions. Journal of Microencapsulation, 21 (6): 615-624.
Zhang Y. Q., Shen W. D., Xiang R. L., Zhuge L. J., Gao W. J., Wang W. B. (2007) Formation of silk fibroin nanoparticles in water-miscible organic solvent and their characterization. Journal of Nanoparticle Research, 9 (5): 885-900.
Gupta V., Aseh A., Ríos C. N., Aggarwal B. B., Mathur A. B. (2009) Fabrication and characterization of silk fibroin-derived curcumin nanoparticles for cancer therapy. International Journal of Nanomedicine, 4: 115.
Calamak S., Aksoy E. A., Ertas N., Erdogdu C., Sagıroglu M., Ulubayram K. (2015) Ag/silk fibroin nanofibers: effect of fibroin morphology on Ag+ release and antibacterial activity. European Polymer Journal, 67: 99-112.
Zhang Q., Yan S., Li M. (2009) Silk fibroin based porous materials. Materials, 2 (4): 2276-2295.
Nagarkar S., Patil A., Lele A., Bhat S., Bellare J., Mashelkar R. A. (2009) Some mechanistic insights into the gelation of regenerated silk fibroin sol. Industrial & Engineering Chemistry Research, 48 (17): 8014-8023.
Yucel T., Cebe P., Kaplan D. L. (2009) Vortex-induced injectable silk fibroin hydrogels. Biophysical Journal, 97 (7): 2044-2050.
Safdari M., Shakiba E., Kiaie S. H., Fattahi A. (2016) Preparation and characterization of Ceftazidime loaded electrospun silk fibroin/gelatin mat for wound dressing. Fibers and Polymers, 17 (5): 744-750.
Wang Y., Kim H. J., Vunjak-Novakovic G., Kaplan D. L. (2006) Stem cell-based tissue engineering with silk biomaterials. Biomaterials, 27 (36): 6064-6082.
Karageorgiou V., Tomkins M., Fajardo R., Meinel L., Snyder B., Wade K., Chen J., Vunjak‐Novakovic G., and Kaplan D. L. (2006) Porous silk fibroin 3‐D scaffolds for delivery of bone morphogenetic protein‐2 in vitro and in vivo. Journal of Biomedical Materials Research Part A, 78 (2): 324-334.
Park C. H., Jeong L., Cho D., Kwon O. H., Park W. H. (2013) Effect of methylcellulose on the formation and drug release behavior of silk fibroin hydrogel. Carbohydrate Polymers, 98 (1): 1179-1185.
Seib F. P., Pritchard E. M., Kaplan D. L. (2013) Self‐assembling doxorubicin silk hydrogels for the focal treatment of primary breast cancer. Advanced Functional Materials, 23 (1): 58-65.
Ebrahimi A., Sadrjavadi K., Hajialyani M., Shokoohinia Y., Fattahi A. (2018) Preparation and characterization of silk fibroin hydrogel as injectable implants for sustained release of Risperidone. Drug Develoment and Industrial Pharmacy, 44 (2): 199-205.
Fatema U. K., Rahman M. M., Islam M. R., Mollah M. Y., Susan M. A. (2018) Silver/poly (vinyl alcohol) nanocomposite film prepared using water in oil microemulsion for antibacterial applications. Journal of Colloid and Interface Science, 514: 648-655.
Hanawa T., Watanabe A., Tsuchiya T., Ikoma R., Hidaka M., Sugihara M. (1995) New oral dosage form for elderly patients: preparation and characterization of silk fibroin gel. Chemical and Pharmaceutical Bulletin, 43 (2): 284-288.
Raut R. W., Kolekar N. S., Lakkakula J. R., Mendhulkar V. D., Kashid S. B., (2010) Extracellular synthesis of silver nanoparticles using dried leaves of Pongamia pinnata (L) pierre. Nano-Micro Letters, 2 (2): 106-113.
Veerasamy R., Xin T. Z., Gunasagaran S., Xiang T. F., Yang E. F., Jeyakumar N., Dhanaraj S. A. (2011) Biosynthesis of silver nanoparticles using mangosteen leaf extract and evaluation of their antimicrobial activities. Journal of Saudi Chemical Society, 15 (2): 113-120.
Ribeiro M., de Moraes M. A., Beppu M. M., Monteiro F. J., Ferraz M. P. (2014) The role of dialysis and freezing on structural conformation, thermal properties and morphology of silk fibroin hydrogels. Biomatterials, 4 (1): 28536.
Ansary R. H., Rahman M. M., Mohamad N., Arrif T. M., Latif A. Z., Katas H., Nik W. S., Awang M. B. (2017) Controlled release of lysozyme from double-walled poly (lactide-co-glycolide) (PLGA) microspheres. Polymers, 9 (10): 485.
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