The interaction of Zn(II) ions with cyanobacteria Spirulina platensis basic protein C-phycocyanin (C-PC) is studied by fluorescence spectroscopy.Stern–Volmer quenching constant value for Zn(II)–C-PC is determined. The binding energy of Zn(II) ions with C-phycocyanin is determined using equilibrium dialysis and atomic absorption spectroscopy. Cooperative binding of Zn(II) ions with C-phycocyanin is observed. The binding constants diminished with increasing ionic strength, suggesting an adaptive protective response. "Nonelectrostatic" and polyelectrolyte components of binding free energy for Ag+, Cu2+, Cr3+, Pb2+, Ni2+, and Zn2+–C-phycocyanin (Spirulina platensis) complexes are determined. It is shown that "nonelectrostatic" component of binding free energy is dominating at the metal–C-PC interaction, while the polyelectrolyte contribution being less important, and the "nonelectrostatic" forces contribution for Ag+–C-phycocyanin (Spirulina platensis) complexes exceeds that for other metal ions.
| Published in |
American Journal of Nano Research and Applications (Volume 5, Issue 3-1)
This article belongs to the Special Issue Nanotechnologies |
| DOI | 10.11648/j.nano.s.2017050301.12 |
| Page(s) | 5-8 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
C-phycocyanin, Zn Ions, Binding Constant
| [1] | D. Bhattacharya and G. Rajinder, “Nanotechnology and potential of microorganisms”, Crit. Rev. Biotechnol., vol. 25, pp. 199–204, 2005. |
| [2] | K. Vijayaraghavan and S. P. Kamala Nalini, “Biotemplates in the green synthesis of silver nanoparticles”, Biotechnol. J., vol. 5, pp. 1098–1110, 2010. |
| [3] | M. Labrenz, G.K. Druschel, E.T. Thomsen, B. Gilbert, S.A. Welch, K.M. Kemner, G.A. Logan, R.E. Summons, G.D. Stasio, P.L. Bond, B. Lai, S.D. Kelly, and J.F. Banfield, “Formation of sphalerite (ZnS) deposits in natural biofilms of sulfate-reducing bacteria,” Science, vol. 290, pp. 1744–1747, 2000. |
| [4] | A.P. Philipse and D. Maas, “Magnetic colloids from magnetotactic bacteria: Chain formation and colloidal stability,” Langmuir, vol. 18, pp. 9977–9984, 2002. |
| [5] | M. Kowshik, W. Vogel, J. Urban, S.K. Kulkarni, and K.M. Paknikar, “Microbial synthesisof semiconductorPbS nanocrystallites,” Adv. Mater., vol. 14, pp. 815–818, 2002. |
| [6] | M. Kowshik, N. Deshmukh, S.K. Kulkarni, K.M. Paknikar, W. Vogel, and J. Urban, “Microbial synthesis of semiconductor CdS nanoparticles, their characterization, and their usein fabrication of an ideal diode,” Biotechnol. Bioeng., vol. 78, pp. 583–588, 2002. |
| [7] | O. Hayashi, S. Ono, K. Ishii, Y. Shi, T. Hirahashi, and T. Katoh, “Enhancement of proliferation and differentiation in bone marrow hematopoietic cells by Spirulina (Arthrospira) platensisin mice”, J. Appl. Phycology, vol. 18, pp. 47–56, 2006. |
| [8] | S.A. Kedik, E.I. Yartsev, I.V. Sakaeva, A.V. Panov, and E.S. Zhavoronok, “Influence of Spirulina andits component on the immune system”, Rus. J. Biopharmaceut., vol. 3, pp.3–10, 2011. |
| [9] | K.K.I.U. Arunakumara and Z. Xuecheng, “Effects of heavy metals (Pb2+ and Cd2+) on the ultra-structure, growth and pigment contents of the unicellular cyanobacterium Synechocystissp. PCC 6803”, Chin. J. Oceanol. Limnol.,vol. 27, pp. 383–388, 2009. |
| [10] | K.K.I.U. Arunakumara, Z. Xuecheng, and S. Xiaojin, “Bioaccumulation of Pb2+ and its effects on growth, morphology and pigment contents of Spirulina (Arthrospira) platensis,” J. Ocean Univ. Chin., vol. 7, pp. 397–403, 2008. |
| [11] | E. Gelagutashvili, “Ch. 9. Biosorption of heavy metals by Spirulina Platensis and their Components,” in Plants and Microbes, P. Goyal, A. Chauhan, and P. Kaushik, Eds., Mumbai, 2014, pp. 154–174. |
| [12] | E. Gelagutashvili, “Binding of heavy metals with C-Phycocyanin: A Comparison between equilibrium dialysis, fluorescence and absorption titration”, Am. J. Biomed. Life Sci., vol.1, pp. 12–16, 2013. |
| [13] | M.R. Eftinkand C.A. Ghiron, “Fluorescence quenching studies with proteins,” Anal. Biochem., vol. 114, pp. 199–227, 1981. |
| [14] | G. Scatchard, “The attraction of proteins for small molecules and ions,” Ann.N.Y.Acad. Sci., vol. 51, pp. 660–672, 1949. |
| [15] | A.V. Hill, “The possible effects of the aggregation of the molecules of hemoglobin on its dissociation curves”, J. Physiol., vol. 40, pp. 463–505, 1910. |
| [16] | Ch.R. Cantor, P.R. Shimmel, Biophysical Chemistry, Part III, Moscow: Mir, 1985. |
| [17] | N.T. Eriksen, “Production of phycocyanin – a pigment with applications in biology, biotechnology, foods and medicine,” Appl. Microbiol. Biotechnol., vol. 80, pp. 1–14, 2008. |
| [18] | A.M. Karshikov, M. Duerring, and R. Huber, “Role of electrostatic interaction in the stability of the hexamer of constitution phycocyanin from Fremyella dislosiphon,” Protein Eng., vol. 4, pp. 681–690, 1991. |
| [19] | M.T. Record and R.S. Spolar, “Some thermodynamic principles of nonspecific and site-specific protein-DNA interactions,” in The Biology of Nonspecific DNA-Protein Interactions, A. Revzin, Ed., Boca Raton: CRC Press, 1990, pp. 33–69. |
| [20] | M.T. Record, J.H. Ha, and M.A. Fisher, “Analysis of equilibrium and kinetic measurements to determine thermodynamic origins of stability and specificity and mechanism of formation of site-specific complexes between proteins and helical DNA,” Methods Enzymol., vol. 208, pp. 291–343, 1991. |
APA Style
Eteri Gelagutashvili. (2016). Cyanobacteria Spirulina Platensis Basic Protein C-Phycocyanin and Zn(II) Ions. American Journal of Nano Research and Applications, 5(3-1), 5-8. https://doi.org/10.11648/j.nano.s.2017050301.12
ACS Style
Eteri Gelagutashvili. Cyanobacteria Spirulina Platensis Basic Protein C-Phycocyanin and Zn(II) Ions. Am. J. Nano Res. Appl. 2016, 5(3-1), 5-8. doi: 10.11648/j.nano.s.2017050301.12
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.nano.s.2017050301.12,
author = {Eteri Gelagutashvili},
title = {Cyanobacteria Spirulina Platensis Basic Protein C-Phycocyanin and Zn(II) Ions},
journal = {American Journal of Nano Research and Applications},
volume = {5},
number = {3-1},
pages = {5-8},
doi = {10.11648/j.nano.s.2017050301.12},
url = {https://doi.org/10.11648/j.nano.s.2017050301.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.nano.s.2017050301.12},
abstract = {The interaction of Zn(II) ions with cyanobacteria Spirulina platensis basic protein C-phycocyanin (C-PC) is studied by fluorescence spectroscopy.Stern–Volmer quenching constant value for Zn(II)–C-PC is determined. The binding energy of Zn(II) ions with C-phycocyanin is determined using equilibrium dialysis and atomic absorption spectroscopy. Cooperative binding of Zn(II) ions with C-phycocyanin is observed. The binding constants diminished with increasing ionic strength, suggesting an adaptive protective response. "Nonelectrostatic" and polyelectrolyte components of binding free energy for Ag+, Cu2+, Cr3+, Pb2+, Ni2+, and Zn2+–C-phycocyanin (Spirulina platensis) complexes are determined. It is shown that "nonelectrostatic" component of binding free energy is dominating at the metal–C-PC interaction, while the polyelectrolyte contribution being less important, and the "nonelectrostatic" forces contribution for Ag+–C-phycocyanin (Spirulina platensis) complexes exceeds that for other metal ions.},
year = {2016}
}
TY - JOUR T1 - Cyanobacteria Spirulina Platensis Basic Protein C-Phycocyanin and Zn(II) Ions AU - Eteri Gelagutashvili Y1 - 2016/09/14 PY - 2016 N1 - https://doi.org/10.11648/j.nano.s.2017050301.12 DO - 10.11648/j.nano.s.2017050301.12 T2 - American Journal of Nano Research and Applications JF - American Journal of Nano Research and Applications JO - American Journal of Nano Research and Applications SP - 5 EP - 8 PB - Science Publishing Group SN - 2575-3738 UR - https://doi.org/10.11648/j.nano.s.2017050301.12 AB - The interaction of Zn(II) ions with cyanobacteria Spirulina platensis basic protein C-phycocyanin (C-PC) is studied by fluorescence spectroscopy.Stern–Volmer quenching constant value for Zn(II)–C-PC is determined. The binding energy of Zn(II) ions with C-phycocyanin is determined using equilibrium dialysis and atomic absorption spectroscopy. Cooperative binding of Zn(II) ions with C-phycocyanin is observed. The binding constants diminished with increasing ionic strength, suggesting an adaptive protective response. "Nonelectrostatic" and polyelectrolyte components of binding free energy for Ag+, Cu2+, Cr3+, Pb2+, Ni2+, and Zn2+–C-phycocyanin (Spirulina platensis) complexes are determined. It is shown that "nonelectrostatic" component of binding free energy is dominating at the metal–C-PC interaction, while the polyelectrolyte contribution being less important, and the "nonelectrostatic" forces contribution for Ag+–C-phycocyanin (Spirulina platensis) complexes exceeds that for other metal ions. VL - 5 IS - 3-1 ER -
Information
Email: