Phytochemical Investigation and Antioxidant Activity of Root Extract of Aloe gilbertii Reynolds, from Konso, Southern Ethiopia
American Journal of Applied Chemistry
Volume 7, Issue 5, October 2019, Pages: 130-136
Received: Sep. 16, 2019; Accepted: Oct. 14, 2019; Published: Oct. 23, 2019
Views 380      Downloads 135
Nebiyu Chali Yadeta, Department of Chemistry, Arba Minch University, Arba Minch, Ethiopia
Article Tools
Follow on us
Aloe gilbertii belonging to the family Aloaceae is used by local people for treatment various disease such as malaria and wound healing. The main purpose of this study was to investigate phytochemicals and antioxidant activity of the Root Extract of Aloe gilbertii Reynolds Phytochemical screening of the CH2Cl2: CH3OH (1:1) extract revealed the presence of flavonoids, anthraquinone, Alkaloids, saponins, phenol and absence of steroids and phytosterols. The anti-oxidant potential and total antioxidant potential was determined by using reducing power determination method and phosphomolybdate method respectively. The antioxidant capacity of methanol, ethanol and ethyl acetate root extracts fractions of Aloe gilbertii were found to be 244.5±0.631, 241.5±0.112 and 106±1.05 mg of ascorbic acid per 10 mg of dry weight of antioxidant in the reducing power, respectively and it’s total antioxidant capacity of these extract fractions were 82.21±0.028, 88.75±0.016 and 74.85±0.043mg of ascorbic acid per 10 mg of dry weight of the crude extract. Thus, the results of the present investigation infer that this plant extracts possess potent antioxidant. Therefore it is strongly recommended to isolate the antioxidant components
Aloaceae, Aloe gilbertii, Antioxidant Potential, FRAP Assay, Total Antioxidant Capacity
To cite this article
Nebiyu Chali Yadeta, Phytochemical Investigation and Antioxidant Activity of Root Extract of Aloe gilbertii Reynolds, from Konso, Southern Ethiopia, American Journal of Applied Chemistry. Vol. 7, No. 5, 2019, pp. 130-136. doi: 10.11648/j.ajac.20190705.11
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Stampfer, M. J. Primary prevention of coronary heart disease in women through diet and lifestyle. New Engl. J. Med. 2000, 343, 16-22.
Hu, F. B. Diet, lifestyle, and the risk of type II diabetes mellitus in women. New Engl. J. Med. 2001, 345, 790-797.
Jaiganesh, K. P., Arunachalam, G., Preliminary Phytochemical Screening And Antimicrobial Potential Of Pterospermum Canescens Roxb, (Sterculiaceae), International Journal of Pharmacy and Pharmaceutical Sciences, 2011, 3 (3), 140.
S., Sharma; H. N., Verma. In-vitro antioxidant activity of seed extracts of Benincasa hispida. J. Nat. Prod. Plant Resour. 2013, 3 (4), 34-37, ISSN: 2231-3184.
Key, T. J. The effect of diet on risk of cancer. Lancet. 2002, 360, 861-868.
S. N., Tyagi; R., Ajeet; A., Saxena; B. D., Patel. In-vitro Antioxidant Activity of Methanolic and Aqueous Extract of Flacourtia induce Merr. Am-Euras. J. Sci. Res. 2010, 5 (3), 201-206.
Ramarathnam, N.; Osawa, T.; Namiki, M.; Kawakishi, S. Chemical studies on novel rice hull antioxidants. Isolation, fractionation, and partial characterization. J. Agric. Food Chem. 1988, 36, 732-737.
Sebsebe Demissew and Nordal, I., (2010). Aloes and Lilies of Ethiopia and Eritrea. ColophonPage. Addis Ababa University andUniversity of Oslo. Pp. 42-109.
Bhattacharjee, S., Paul, S., Dutta, S., and Chaudhuri, T. K. 2014. Antiinflammatory and protective properties of Aloe vera leaf crude gel in carrageenan induced acute inflammatory rat models. Int. J. Pharmacy and Pharmaceutical Sci., 6 (9): 368-371.
Balemie K, Kelbessa E, Asfaw Z: Indigenous Medicinal Plant Utilization, Management and Threats in Fentalle Area, Eastern Shewa, Ethiopia. Ethiopian Journal of Biological Science. 2004, 3 (1): 37-58.
Cunningham AB: African Medicinal Plants: Setting Priorities at the Interface between Conservation and Primary Healthcare People and Plants Working Paper 1. Paris; 1993.
Hill AF: Economic Botany New Delhi: TATA McGRAW-HILL PUBLISHING COMPANY LTD; 1989.
Bolling, B.; Dolnikowski, G.; Blumberg, J.; Chen, C. Y. Food Chemistry., 2010, 122, 819-825.
Md., Wasim; J. F., Ayala-Zavala; R. S., Dhua. Genotypic variation in tomatoes affecting processing and antioxidant attributes. Crit. Rev. Food Sci. Nutr. 2013.
Ming-Chih, S.; Cheng-Ming, C.; Sue-Ming, K.; Min-Lang, T. Effect of Different Parts (Leaf, Stem and Stalk) and Seasons (summer and winter) on the Chemical Compositions and Antioxidant Activity of Moringa oleifera. Int. J. Mol. Sci. 2011, 12, 6077-6088.
M., Naczk; R., Amarowicz; R., Zadernowski; Ronald B., Pegg; F., Shahidi. Antioxidant activity of crude phenolic extracts from wild blueberry leaves. Pol. J. Food Nutr. Sci. 2003, Vol. 12/53, SI 1, pp. 166–169.
Sayed, A.; El-toumy, S. M.; Mohamed, E.; Abdel-Tawab, H. M. Phenolic Metabolites from Acacia nilotica Flowers and Evaluation of its Free Radical Scavenging Activity. Journal of American Science, 2011, 7 (3).
Obasi NL, Egbuonu ACC, Ukoha PO, Ejikeme PM. Comparative phytochemical and antimicrobial screening of some solvent extracts of Samaneasamanpods. African journal of pure and applied chemistry 2010; 4 (9): 206-212.
Dagne, E., Bisrat, D., Viljoen, A. &VanWyk, B. E. 2000. Chemistry of Aloe species. Current Organic Chemistry. 4 (10): 1055-1078.
Fikre Dessalegn (2013). study on the population of endemic aloe species (A. gilbertii Reynolds) in Ethiopia.
Irshad, Md.; Zafaryab, Md.; Man Singh, M. Moshahid, A. Int. J. Med. chem., 2012, Article ID 157125, 6 pages.
Ming-Chih, S.; Cheng-Ming, C.; Sue-Ming, K.; Min-Lang, T. Int. J. Mol. Sci., 2011, 12, 6077-6088.
Sethi PD: HPTLC Quantitative Analysis of Pharmaceutical Formulations, 1sted. CBS Publishers and Distributors, NewDelhi. 1996; 3-73. Roopashree TS, Dang R, Rani SRH, Narendra C. Antibacterial activity of anti-psoriatic herbs: Cassia tora, Momordicacharantia and Calendula officinalis. International Journal of Applied Research in Natural Products. 2008; 1 (3): 20-28.
Evans WC. Trease and Evans Pharmacognosy. General methods associated with the phytochemical investigation of herbal products. 2002; pp 139-143.
Atawodi, S. E. Evaluation of the polyphenol content and Antioxidant properties of methanol extracts of the leaves, stem, and root barks of Moringa oleifera Lam. J. Med. Food. 2010, 13 (3), 710-716.
Asmare, A. and Kesara, Na-B. Ch. Afr. J. Pharm. Pharmacol., 2015, 9 (25), pp. 615-627.
Cho, E. J.; Yokozawa, T.; Rhyu, D. Y.; Kim, S. C.; Shibahara, N.; Park, J. C. Phytomedicine., 2003, 10, 544.
Aberra, M.; Workinesh, T; Tegene, N. Effects of feeding Moringa stenopetala leaf meal on nutrient intake and growth performance of Rhode Island Red chicks under tropical climate Subtrop. Agroecosyst. 2013, 14, 485-492.
Mekonnen, Y. The multi–purpose Moringa tree in Ethiopia. Examples of the development of pharmaceutical products from medicinal plants. UN development program, New York. 2011, 10, 111-117.
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
Tel: (001)347-983-5186