Changes in Haematological Parameters Following Toxicity Study with 80% Methanol Extract of <i>Moringa oleifera</i> in Wistar Rats

Ibrahim Maina Hassan; Bashir Saidu; Ja’afaru Abdullahi Ishaq; Ashiru Dahiru; Nafisat Abdulazeez; Halima Ibrahim Yusuf; Ahmadu Abdulrahman Shekwaye; Muhammad Bashir Bello; Adamu Abdul Abubakar; Yushau Shuaibu Baraya

doi:doi:10.11648/j.ajac.20200806.13

Changes in Haematological Parameters Following Toxicity Study with 80% Methanol Extract of Moringa oleifera in Wistar Rats

Ibrahim Maina Hassan, Bashir Saidu, Ja’afaru Abdullahi Ishaq, Ashiru Dahiru, Nafisat Abdulazeez, Halima Ibrahim Yusuf, Ahmadu Abdulrahman Shekwaye, Muhammad Bashir Bello, Adamu Abdul Abubakar, Yushau Shuaibu Baraya

Published in American Journal of Applied Chemistry (Volume 8, Issue 6)

Received: 27 March 2020 Accepted: 15 April 2020 Published: 31 December 2020

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Abstract

To determine the acute toxicity effects of Moringa oleifera leaf extract on haematological parameters, twenty albino Wistar rats weighing between 108-254 g were split into four groups of fives rat each. Different doses of the extract were administered to the 3 groups and one group is used as control. Moringa oleifera 80% methanol leaf extract was administered at 2000 mg/kg to group, 1000 mg/kg to group, and 500 mg/kg while one group was used as control. The result recorded 4 deaths (60% mortality) in groups that received 2000 mg/kg and 1 death (20% mortality) in groups that received 1000 mg/kg. Blood samples were collected from the survived rats for hematological analysis. The result showed variation in Park cell volume (PCV), hemoglobin concentration (Hb), Red blood cells (RBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC). There are significant differences at p > 0.05, p > 0.01 and p > 0.001 between groups that exposed to different doses Moringa oleifera extract and the control group. The result also shows slight variation in total and differential leucocyte count, but all the values obtained from both treated and control groups are within the normal range. There are no recorded values of eosinophil, basophil and band cells in both the control and the experimental groups. From this result, it can be concluded that high dose of this extract caused alteration in normal red cells indices with significant differences, but have mild or no effect on total and differential leucocyte count. Hence, low toxicity of this plant made it safe and good medicinal agent and or supplement. Histopatological screening as well as phytochemical study to identify the compounds that affect the blood chemistry is highly recommended.

Published in	American Journal of Applied Chemistry (Volume 8, Issue 6)
DOI	10.11648/j.ajac.20200806.13
Page(s)	143-151
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Hemoglobin Concentration, Leucocyte, Mean Corpuscular Hemoglobin, Mean Corpuscular Hemoglobin Concentration, Mean Corpuscular Volume, Park Cell Volume, Red Blood Cells

References

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Ibrahim Maina Hassan, Bashir Saidu, Ja’afaru Abdullahi Ishaq, Ashiru Dahiru, Nafisat Abdulazeez, et al. (2020). Changes in Haematological Parameters Following Toxicity Study with 80% Methanol Extract of Moringa oleifera in Wistar Rats. American Journal of Applied Chemistry, 8(6), 143-151. https://doi.org/10.11648/j.ajac.20200806.13

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ACS Style

Ibrahim Maina Hassan; Bashir Saidu; Ja’afaru Abdullahi Ishaq; Ashiru Dahiru; Nafisat Abdulazeez, et al. Changes in Haematological Parameters Following Toxicity Study with 80% Methanol Extract of Moringa oleifera in Wistar Rats. Am. J. Appl. Chem. 2020, 8(6), 143-151. doi: 10.11648/j.ajac.20200806.13

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AMA Style

Ibrahim Maina Hassan, Bashir Saidu, Ja’afaru Abdullahi Ishaq, Ashiru Dahiru, Nafisat Abdulazeez, et al. Changes in Haematological Parameters Following Toxicity Study with 80% Methanol Extract of Moringa oleifera in Wistar Rats. Am J Appl Chem. 2020;8(6):143-151. doi: 10.11648/j.ajac.20200806.13

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@article{10.11648/j.ajac.20200806.13,
  author = {Ibrahim Maina Hassan and Bashir Saidu and Ja’afaru Abdullahi Ishaq and Ashiru Dahiru and Nafisat Abdulazeez and Halima Ibrahim Yusuf and Ahmadu Abdulrahman Shekwaye and Muhammad Bashir Bello and Adamu Abdul Abubakar and Yushau Shuaibu Baraya},
  title = {Changes in Haematological Parameters Following Toxicity Study with 80% Methanol Extract of Moringa oleifera in Wistar Rats},
  journal = {American Journal of Applied Chemistry},
  volume = {8},
  number = {6},
  pages = {143-151},
  doi = {10.11648/j.ajac.20200806.13},
  url = {https://doi.org/10.11648/j.ajac.20200806.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajac.20200806.13},
  abstract = {To determine the acute toxicity effects of Moringa oleifera leaf extract on haematological parameters, twenty albino Wistar rats weighing between 108-254 g were split into four groups of fives rat each. Different doses of the extract were administered to the 3 groups and one group is used as control. Moringa oleifera 80% methanol leaf extract was administered at 2000 mg/kg to group, 1000 mg/kg to group, and 500 mg/kg while one group was used as control. The result recorded 4 deaths (60% mortality) in groups that received 2000 mg/kg and 1 death (20% mortality) in groups that received 1000 mg/kg. Blood samples were collected from the survived rats for hematological analysis. The result showed variation in Park cell volume (PCV), hemoglobin concentration (Hb), Red blood cells (RBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC). There are significant differences at p > 0.05, p > 0.01 and p > 0.001 between groups that exposed to different doses Moringa oleifera extract and the control group. The result also shows slight variation in total and differential leucocyte count, but all the values obtained from both treated and control groups are within the normal range. There are no recorded values of eosinophil, basophil and band cells in both the control and the experimental groups. From this result, it can be concluded that high dose of this extract caused alteration in normal red cells indices with significant differences, but have mild or no effect on total and differential leucocyte count. Hence, low toxicity of this plant made it safe and good medicinal agent and or supplement. Histopatological screening as well as phytochemical study to identify the compounds that affect the blood chemistry is highly recommended.},
 year = {2020}
}

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TY  - JOUR
T1  - Changes in Haematological Parameters Following Toxicity Study with 80% Methanol Extract of Moringa oleifera in Wistar Rats
AU  - Ibrahim Maina Hassan
AU  - Bashir Saidu
AU  - Ja’afaru Abdullahi Ishaq
AU  - Ashiru Dahiru
AU  - Nafisat Abdulazeez
AU  - Halima Ibrahim Yusuf
AU  - Ahmadu Abdulrahman Shekwaye
AU  - Muhammad Bashir Bello
AU  - Adamu Abdul Abubakar
AU  - Yushau Shuaibu Baraya
Y1  - 2020/12/31
PY  - 2020
N1  - https://doi.org/10.11648/j.ajac.20200806.13
DO  - 10.11648/j.ajac.20200806.13
T2  - American Journal of Applied Chemistry
JF  - American Journal of Applied Chemistry
JO  - American Journal of Applied Chemistry
SP  - 143
EP  - 151
PB  - Science Publishing Group
SN  - 2330-8745
UR  - https://doi.org/10.11648/j.ajac.20200806.13
AB  - To determine the acute toxicity effects of Moringa oleifera leaf extract on haematological parameters, twenty albino Wistar rats weighing between 108-254 g were split into four groups of fives rat each. Different doses of the extract were administered to the 3 groups and one group is used as control. Moringa oleifera 80% methanol leaf extract was administered at 2000 mg/kg to group, 1000 mg/kg to group, and 500 mg/kg while one group was used as control. The result recorded 4 deaths (60% mortality) in groups that received 2000 mg/kg and 1 death (20% mortality) in groups that received 1000 mg/kg. Blood samples were collected from the survived rats for hematological analysis. The result showed variation in Park cell volume (PCV), hemoglobin concentration (Hb), Red blood cells (RBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC). There are significant differences at p > 0.05, p > 0.01 and p > 0.001 between groups that exposed to different doses Moringa oleifera extract and the control group. The result also shows slight variation in total and differential leucocyte count, but all the values obtained from both treated and control groups are within the normal range. There are no recorded values of eosinophil, basophil and band cells in both the control and the experimental groups. From this result, it can be concluded that high dose of this extract caused alteration in normal red cells indices with significant differences, but have mild or no effect on total and differential leucocyte count. Hence, low toxicity of this plant made it safe and good medicinal agent and or supplement. Histopatological screening as well as phytochemical study to identify the compounds that affect the blood chemistry is highly recommended.
VL  - 8
IS  - 6
ER  -

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Author Information

Ibrahim Maina Hassan

Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria
Bashir Saidu

Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria
Ja’afaru Abdullahi Ishaq

Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria
Ashiru Dahiru

Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria
Nafisat Abdulazeez

Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria
Halima Ibrahim Yusuf

Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria
Ahmadu Abdulrahman Shekwaye

Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria
Muhammad Bashir Bello

Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria
Adamu Abdul Abubakar

Department of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria
Yushau Shuaibu Baraya

Department of Veterinary Pathology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria

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Ibrahim Maina Hassan, Bashir Saidu, Ja’afaru Abdullahi Ishaq, Ashiru Dahiru, Nafisat Abdulazeez, et al. (2020). Changes in Haematological Parameters Following Toxicity Study with 80% Methanol Extract of Moringa oleifera in Wistar Rats. American Journal of Applied Chemistry, 8(6), 143-151. https://doi.org/10.11648/j.ajac.20200806.13

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ACS Style

Ibrahim Maina Hassan; Bashir Saidu; Ja’afaru Abdullahi Ishaq; Ashiru Dahiru; Nafisat Abdulazeez, et al. Changes in Haematological Parameters Following Toxicity Study with 80% Methanol Extract of Moringa oleifera in Wistar Rats. Am. J. Appl. Chem. 2020, 8(6), 143-151. doi: 10.11648/j.ajac.20200806.13

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AMA Style

Ibrahim Maina Hassan, Bashir Saidu, Ja’afaru Abdullahi Ishaq, Ashiru Dahiru, Nafisat Abdulazeez, et al. Changes in Haematological Parameters Following Toxicity Study with 80% Methanol Extract of Moringa oleifera in Wistar Rats. Am J Appl Chem. 2020;8(6):143-151. doi: 10.11648/j.ajac.20200806.13

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@article{10.11648/j.ajac.20200806.13,
  author = {Ibrahim Maina Hassan and Bashir Saidu and Ja’afaru Abdullahi Ishaq and Ashiru Dahiru and Nafisat Abdulazeez and Halima Ibrahim Yusuf and Ahmadu Abdulrahman Shekwaye and Muhammad Bashir Bello and Adamu Abdul Abubakar and Yushau Shuaibu Baraya},
  title = {Changes in Haematological Parameters Following Toxicity Study with 80% Methanol Extract of Moringa oleifera in Wistar Rats},
  journal = {American Journal of Applied Chemistry},
  volume = {8},
  number = {6},
  pages = {143-151},
  doi = {10.11648/j.ajac.20200806.13},
  url = {https://doi.org/10.11648/j.ajac.20200806.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajac.20200806.13},
  abstract = {To determine the acute toxicity effects of Moringa oleifera leaf extract on haematological parameters, twenty albino Wistar rats weighing between 108-254 g were split into four groups of fives rat each. Different doses of the extract were administered to the 3 groups and one group is used as control. Moringa oleifera 80% methanol leaf extract was administered at 2000 mg/kg to group, 1000 mg/kg to group, and 500 mg/kg while one group was used as control. The result recorded 4 deaths (60% mortality) in groups that received 2000 mg/kg and 1 death (20% mortality) in groups that received 1000 mg/kg. Blood samples were collected from the survived rats for hematological analysis. The result showed variation in Park cell volume (PCV), hemoglobin concentration (Hb), Red blood cells (RBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC). There are significant differences at p > 0.05, p > 0.01 and p > 0.001 between groups that exposed to different doses Moringa oleifera extract and the control group. The result also shows slight variation in total and differential leucocyte count, but all the values obtained from both treated and control groups are within the normal range. There are no recorded values of eosinophil, basophil and band cells in both the control and the experimental groups. From this result, it can be concluded that high dose of this extract caused alteration in normal red cells indices with significant differences, but have mild or no effect on total and differential leucocyte count. Hence, low toxicity of this plant made it safe and good medicinal agent and or supplement. Histopatological screening as well as phytochemical study to identify the compounds that affect the blood chemistry is highly recommended.},
 year = {2020}
}

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TY  - JOUR
T1  - Changes in Haematological Parameters Following Toxicity Study with 80% Methanol Extract of Moringa oleifera in Wistar Rats
AU  - Ibrahim Maina Hassan
AU  - Bashir Saidu
AU  - Ja’afaru Abdullahi Ishaq
AU  - Ashiru Dahiru
AU  - Nafisat Abdulazeez
AU  - Halima Ibrahim Yusuf
AU  - Ahmadu Abdulrahman Shekwaye
AU  - Muhammad Bashir Bello
AU  - Adamu Abdul Abubakar
AU  - Yushau Shuaibu Baraya
Y1  - 2020/12/31
PY  - 2020
N1  - https://doi.org/10.11648/j.ajac.20200806.13
DO  - 10.11648/j.ajac.20200806.13
T2  - American Journal of Applied Chemistry
JF  - American Journal of Applied Chemistry
JO  - American Journal of Applied Chemistry
SP  - 143
EP  - 151
PB  - Science Publishing Group
SN  - 2330-8745
UR  - https://doi.org/10.11648/j.ajac.20200806.13
AB  - To determine the acute toxicity effects of Moringa oleifera leaf extract on haematological parameters, twenty albino Wistar rats weighing between 108-254 g were split into four groups of fives rat each. Different doses of the extract were administered to the 3 groups and one group is used as control. Moringa oleifera 80% methanol leaf extract was administered at 2000 mg/kg to group, 1000 mg/kg to group, and 500 mg/kg while one group was used as control. The result recorded 4 deaths (60% mortality) in groups that received 2000 mg/kg and 1 death (20% mortality) in groups that received 1000 mg/kg. Blood samples were collected from the survived rats for hematological analysis. The result showed variation in Park cell volume (PCV), hemoglobin concentration (Hb), Red blood cells (RBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC). There are significant differences at p > 0.05, p > 0.01 and p > 0.001 between groups that exposed to different doses Moringa oleifera extract and the control group. The result also shows slight variation in total and differential leucocyte count, but all the values obtained from both treated and control groups are within the normal range. There are no recorded values of eosinophil, basophil and band cells in both the control and the experimental groups. From this result, it can be concluded that high dose of this extract caused alteration in normal red cells indices with significant differences, but have mild or no effect on total and differential leucocyte count. Hence, low toxicity of this plant made it safe and good medicinal agent and or supplement. Histopatological screening as well as phytochemical study to identify the compounds that affect the blood chemistry is highly recommended.
VL  - 8
IS  - 6
ER  -

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