Analytical Method Development and Validation of Simultaneous Estimation of Paracetamol, Aceclofenac and Serratiopeptidase by RP-HPLC

Bhavani Podili; Mohan Seelam; Prasada Rao Kammela

doi:doi:10.11648/j.ijovs.20170203.12

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Analytical Method Development and Validation of Simultaneous Estimation of Paracetamol, Aceclofenac and Serratiopeptidase by RP-HPLC

Bhavani Podili, Mohan Seelam, Prasada Rao Kammela

Published in International Journal of Ophthalmology & Visual Science (Volume 2, Issue 3)

Received: 30 March 2017 Accepted: 24 April 2017 Published: 19 June 2017

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Abstract

In this study, high performance chromatographic method have been developed and validated for the estimation of Paracetamol (PC), Aceclofenac (AF), and Serratio peptidase (SP) in combined tablet dosage form. The chromatography was carried out on a phenomenex C18 (Luna) coloumn (250mmX 4.6mm, 5µm) with a mobile phase consisting of buffer containing 1gm heptanesulfonic acid in 1lit water (adjusted to pH 2.5 with ortho phosphoric acid) acetonitrile (90:10 v/v) at a flow rate of 1.0 mL/min and ultraviolet detection at 226 nm. The retention time of PC, AF and SP were 3.119, 7.196 and 13.560 minutes respectively. Validation of the proposed method was carried out according to International Conference on Harmonisation (ICH) guidelines. Linearity range was obtained for PC, AF and SPover the concentration range of 2.1-31.5, 0.66-9.90 and 0.1-1.5 µg/mL and the r2 values were 0.9995, 0.9998 and 0.9992 respectively. The calculate limit of detection (LOD) values were 0.21, 0.066, and 0.01µg/mL and limit of quantitation (LOQ) values were 0.42, 0.132 and 0.02µg/mL for PC, AF, and SP correspondingly.

Published in	International Journal of Ophthalmology & Visual Science (Volume 2, Issue 3)
DOI	10.11648/j.ijovs.20170203.12
Page(s)	69-74
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Paracetamol (PC), Aceclofenac (AF), Serratio Peptidase (SP), RP-HPLC

References

[1]	N. Y. Hasan, M. Abdel-Elkawy, B. E. Elzeany, N. E. Wagieh, Farmaco, 2003, 58, 91.
[2]	Y. S. El-Saharty, M. Refaat, S. Z. El-Khateeb, Drug. Develop. Ind. Pharm, 2002, 28, 571.
[3]	N. H. Zawilla, M. A. Mohammad, N. M. El-Kousy, S. M. El-MoghazyAly, J. P harm. Biomed. Anal, 2002, 27, 243.
[4]	B. Hinz, D. Auge, T. Rau, S. Reitbrock, K. Brune, U. Werner, Biomed. Chromatogr, 2003, 17, 268.
[5]	XQ. Liu, X. J. Chen, L. H. Zhao, J. H. Peng, Yao XueBao, 1997, 32, 546.
[6]	H. S. Lee, C. K. Jeong, S. J. Choi, S. B. Kim, M. H. Lee, G. I. Ko, D. H. Sohn, J. Pharm. Biomed. Anal, 2000, 23, 775.
[7]	N. M. El-Kousy, J. Pharm. Biomed. Anal, 1999, 20, 185.
[8]	L. S. Jensen, J. Valentine, R. W. Milne, A. M. Evans, J. Pharm. Biomed. Anal, 2004, 34, 585.
[9]	M. Ohta, N. Kawakami, S. Yamato, K. Shimada, J. Pharm. Biomed. Anal, 2003, 15, 1759.
[10]	P. Ortega-Barrales, R. Padilla-Weigand, A. Molina-Diaz, Anal. Sci, 2002, 18, 1241.
[11]	E. Dnic, C. Yucesoy, F. Onur, J. Pharm. Biomed. Anal, 2002, 15, 1091.
[12]	J. E. F. Reynolds,\Martindale The Extra Pharmacopoeia", 31st ed., pp. 27-28, Pharmaceutical Press, London, 1996.
[13]	The United States Pharmacopoeia, 24th revision, pp. 17-39, U. S. Pharmacopeial Convention, Rockville, MD, 2000.
[14]	The Indian pharmacopoeia commission “Indian Pharmacopoeia” (2010), Ghaziabad 1859, 2230.
[15]	Dissolution Profile of Aceclofenac Tablets Available in Bangladesh. S. J Pharm Sci. 2010, 3(1), 1-3.
[16]	R. Sivakumar, P. E. Venkataraman, R. Natarajan, V. Ganeasn, K. A. A. Ali, Comparative in vitro Evaluation of Commercial Aceclofenac Tablets. Int. J Chem Sci. 2009, 7(3), 1591-1594.
[17]	K. Raza, M. Kumar, P. Kumar, R. Malik, G. Sharma, M. Kauret al. Topical Delivery of Aceclofenac: Challenges and Promises of Novel Drug Delivery Systems. Biomed Res. Int. 2014, 2014: 1-11.
[18]	R. Yamazaki, S. Kawai, T. Matsuzaki, N. Kaneda, S. Hashimoto, T. Yokokuraet al. Aceclofenac blocks prostaglandin E2 production following its intracellular conversion into cyclooxygenase inhibitors. Eur. J Pharmacol. 1997, 329(2-3), 181-187.
[19]	Brogden RN, Wiseman LR. Aceclofenac: a review of itspharmacodynamic properties and therapeutic potential inthe treatment of rheumatic disorders and in painmanagement. Drugs. 1996, 52(1), 113-124.
[20]	K. Nakahama, K. Yoshimura, R. Marumoto, M. Kikuchi, IS. Lee, T. Hase, H. Matsubara, Clining and sequencing of Serratiaproteasegene, Nucleic Acids Res, 1986, 14(14 ) 5843-55.
[21]	M. Isono, M. Kazutaka, R. Kodama, K. Tomoda, K. Miyata. Serratiaprotease Part I. Purification and general properties of the enzyme, Agricultural and Biological Chemistry, 1970, 34(2), 310-318.
[22]	C. K. Kokate, A. P. Purohit, S. B. Gokhale, Pharmacognosy, 14th ed. NiraliPrakashan, Mumbai, 2008, 12. 11.

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APA Style

Bhavani Podili, Mohan Seelam, Prasada Rao Kammela. (2017). Analytical Method Development and Validation of Simultaneous Estimation of Paracetamol, Aceclofenac and Serratiopeptidase by RP-HPLC. International Journal of Ophthalmology & Visual Science, 2(3), 69-74. https://doi.org/10.11648/j.ijovs.20170203.12

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ACS Style

Bhavani Podili; Mohan Seelam; Prasada Rao Kammela. Analytical Method Development and Validation of Simultaneous Estimation of Paracetamol, Aceclofenac and Serratiopeptidase by RP-HPLC. Int. J. Ophthalmol. Vis. Sci. 2017, 2(3), 69-74. doi: 10.11648/j.ijovs.20170203.12

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AMA Style

Bhavani Podili, Mohan Seelam, Prasada Rao Kammela. Analytical Method Development and Validation of Simultaneous Estimation of Paracetamol, Aceclofenac and Serratiopeptidase by RP-HPLC. Int J Ophthalmol Vis Sci. 2017;2(3):69-74. doi: 10.11648/j.ijovs.20170203.12

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@article{10.11648/j.ijovs.20170203.12,
  author = {Bhavani Podili and Mohan Seelam and Prasada Rao Kammela},
  title = {Analytical Method Development and Validation of Simultaneous Estimation of Paracetamol, Aceclofenac and Serratiopeptidase by RP-HPLC},
  journal = {International Journal of Ophthalmology & Visual Science},
  volume = {2},
  number = {3},
  pages = {69-74},
  doi = {10.11648/j.ijovs.20170203.12},
  url = {https://doi.org/10.11648/j.ijovs.20170203.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijovs.20170203.12},
  abstract = {In this study, high performance chromatographic method have been developed and validated for the estimation of Paracetamol (PC), Aceclofenac (AF), and Serratio peptidase (SP) in combined tablet dosage form. The chromatography was carried out on a phenomenex C18 (Luna) coloumn (250mmX 4.6mm, 5µm) with a mobile phase consisting of buffer containing 1gm heptanesulfonic acid in 1lit water (adjusted to pH 2.5 with ortho phosphoric acid) acetonitrile (90:10 v/v) at a flow rate of 1.0 mL/min and ultraviolet detection at 226 nm. The retention time of PC, AF and SP were 3.119, 7.196 and 13.560 minutes respectively. Validation of the proposed method was carried out according to International Conference on Harmonisation (ICH) guidelines. Linearity range was obtained for PC, AF and SPover the concentration range of 2.1-31.5, 0.66-9.90 and 0.1-1.5 µg/mL and the r2 values were 0.9995, 0.9998 and 0.9992 respectively. The calculate limit of detection (LOD) values were 0.21, 0.066, and 0.01µg/mL and limit of quantitation (LOQ) values were 0.42, 0.132 and 0.02µg/mL for PC, AF, and SP correspondingly.},
 year = {2017}
}

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TY  - JOUR
T1  - Analytical Method Development and Validation of Simultaneous Estimation of Paracetamol, Aceclofenac and Serratiopeptidase by RP-HPLC
AU  - Bhavani Podili
AU  - Mohan Seelam
AU  - Prasada Rao Kammela
Y1  - 2017/06/19
PY  - 2017
N1  - https://doi.org/10.11648/j.ijovs.20170203.12
DO  - 10.11648/j.ijovs.20170203.12
T2  - International Journal of Ophthalmology & Visual Science
JF  - International Journal of Ophthalmology & Visual Science
JO  - International Journal of Ophthalmology & Visual Science
SP  - 69
EP  - 74
PB  - Science Publishing Group
SN  - 2637-3858
UR  - https://doi.org/10.11648/j.ijovs.20170203.12
AB  - In this study, high performance chromatographic method have been developed and validated for the estimation of Paracetamol (PC), Aceclofenac (AF), and Serratio peptidase (SP) in combined tablet dosage form. The chromatography was carried out on a phenomenex C18 (Luna) coloumn (250mmX 4.6mm, 5µm) with a mobile phase consisting of buffer containing 1gm heptanesulfonic acid in 1lit water (adjusted to pH 2.5 with ortho phosphoric acid) acetonitrile (90:10 v/v) at a flow rate of 1.0 mL/min and ultraviolet detection at 226 nm. The retention time of PC, AF and SP were 3.119, 7.196 and 13.560 minutes respectively. Validation of the proposed method was carried out according to International Conference on Harmonisation (ICH) guidelines. Linearity range was obtained for PC, AF and SPover the concentration range of 2.1-31.5, 0.66-9.90 and 0.1-1.5 µg/mL and the r2 values were 0.9995, 0.9998 and 0.9992 respectively. The calculate limit of detection (LOD) values were 0.21, 0.066, and 0.01µg/mL and limit of quantitation (LOQ) values were 0.42, 0.132 and 0.02µg/mL for PC, AF, and SP correspondingly.
VL  - 2
IS  - 3
ER  -

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Author Information

Bhavani Podili

Department of Chemistry, Acharya Nagarjuna University, Guntur, India
Mohan Seelam

Department of Chemistry, Bapatla Engineering College, Bapatla, India
Prasada Rao Kammela

Department of Chemistry, Bapatla Engineering College, Bapatla, India

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APA Style

Bhavani Podili, Mohan Seelam, Prasada Rao Kammela. (2017). Analytical Method Development and Validation of Simultaneous Estimation of Paracetamol, Aceclofenac and Serratiopeptidase by RP-HPLC. International Journal of Ophthalmology & Visual Science, 2(3), 69-74. https://doi.org/10.11648/j.ijovs.20170203.12

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ACS Style

Bhavani Podili; Mohan Seelam; Prasada Rao Kammela. Analytical Method Development and Validation of Simultaneous Estimation of Paracetamol, Aceclofenac and Serratiopeptidase by RP-HPLC. Int. J. Ophthalmol. Vis. Sci. 2017, 2(3), 69-74. doi: 10.11648/j.ijovs.20170203.12

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AMA Style

Bhavani Podili, Mohan Seelam, Prasada Rao Kammela. Analytical Method Development and Validation of Simultaneous Estimation of Paracetamol, Aceclofenac and Serratiopeptidase by RP-HPLC. Int J Ophthalmol Vis Sci. 2017;2(3):69-74. doi: 10.11648/j.ijovs.20170203.12

Copy | Download

@article{10.11648/j.ijovs.20170203.12,
  author = {Bhavani Podili and Mohan Seelam and Prasada Rao Kammela},
  title = {Analytical Method Development and Validation of Simultaneous Estimation of Paracetamol, Aceclofenac and Serratiopeptidase by RP-HPLC},
  journal = {International Journal of Ophthalmology & Visual Science},
  volume = {2},
  number = {3},
  pages = {69-74},
  doi = {10.11648/j.ijovs.20170203.12},
  url = {https://doi.org/10.11648/j.ijovs.20170203.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijovs.20170203.12},
  abstract = {In this study, high performance chromatographic method have been developed and validated for the estimation of Paracetamol (PC), Aceclofenac (AF), and Serratio peptidase (SP) in combined tablet dosage form. The chromatography was carried out on a phenomenex C18 (Luna) coloumn (250mmX 4.6mm, 5µm) with a mobile phase consisting of buffer containing 1gm heptanesulfonic acid in 1lit water (adjusted to pH 2.5 with ortho phosphoric acid) acetonitrile (90:10 v/v) at a flow rate of 1.0 mL/min and ultraviolet detection at 226 nm. The retention time of PC, AF and SP were 3.119, 7.196 and 13.560 minutes respectively. Validation of the proposed method was carried out according to International Conference on Harmonisation (ICH) guidelines. Linearity range was obtained for PC, AF and SPover the concentration range of 2.1-31.5, 0.66-9.90 and 0.1-1.5 µg/mL and the r2 values were 0.9995, 0.9998 and 0.9992 respectively. The calculate limit of detection (LOD) values were 0.21, 0.066, and 0.01µg/mL and limit of quantitation (LOQ) values were 0.42, 0.132 and 0.02µg/mL for PC, AF, and SP correspondingly.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Analytical Method Development and Validation of Simultaneous Estimation of Paracetamol, Aceclofenac and Serratiopeptidase by RP-HPLC
AU  - Bhavani Podili
AU  - Mohan Seelam
AU  - Prasada Rao Kammela
Y1  - 2017/06/19
PY  - 2017
N1  - https://doi.org/10.11648/j.ijovs.20170203.12
DO  - 10.11648/j.ijovs.20170203.12
T2  - International Journal of Ophthalmology & Visual Science
JF  - International Journal of Ophthalmology & Visual Science
JO  - International Journal of Ophthalmology & Visual Science
SP  - 69
EP  - 74
PB  - Science Publishing Group
SN  - 2637-3858
UR  - https://doi.org/10.11648/j.ijovs.20170203.12
AB  - In this study, high performance chromatographic method have been developed and validated for the estimation of Paracetamol (PC), Aceclofenac (AF), and Serratio peptidase (SP) in combined tablet dosage form. The chromatography was carried out on a phenomenex C18 (Luna) coloumn (250mmX 4.6mm, 5µm) with a mobile phase consisting of buffer containing 1gm heptanesulfonic acid in 1lit water (adjusted to pH 2.5 with ortho phosphoric acid) acetonitrile (90:10 v/v) at a flow rate of 1.0 mL/min and ultraviolet detection at 226 nm. The retention time of PC, AF and SP were 3.119, 7.196 and 13.560 minutes respectively. Validation of the proposed method was carried out according to International Conference on Harmonisation (ICH) guidelines. Linearity range was obtained for PC, AF and SPover the concentration range of 2.1-31.5, 0.66-9.90 and 0.1-1.5 µg/mL and the r2 values were 0.9995, 0.9998 and 0.9992 respectively. The calculate limit of detection (LOD) values were 0.21, 0.066, and 0.01µg/mL and limit of quantitation (LOQ) values were 0.42, 0.132 and 0.02µg/mL for PC, AF, and SP correspondingly.
VL  - 2
IS  - 3
ER  -

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