Analytical Method Development and Validation of Simultaneous Estimation of Paracetamol, Aceclofenac and Serratiopeptidase by RP-HPLC
International Journal of Ophthalmology & Visual Science
Volume 2, Issue 3, August 2017, Pages: 69-74
Received: Mar. 30, 2017;
Accepted: Apr. 24, 2017;
Published: Jun. 19, 2017
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Bhavani Podili, Department of Chemistry, Acharya Nagarjuna University, Guntur, India
Mohan Seelam, Department of Chemistry, Bapatla Engineering College, Bapatla, India
Prasada Rao Kammela, Department of Chemistry, Bapatla Engineering College, Bapatla, India
In this study, high performance chromatographic method have been developed and validated for the estimation of Paracetamol (PC), Aceclofenac (AF), and Serratio peptidase (SP) in combined tablet dosage form. The chromatography was carried out on a phenomenex C18 (Luna) coloumn (250mmX 4.6mm, 5µm) with a mobile phase consisting of buffer containing 1gm heptanesulfonic acid in 1lit water (adjusted to pH 2.5 with ortho phosphoric acid) acetonitrile (90:10 v/v) at a flow rate of 1.0 mL/min and ultraviolet detection at 226 nm. The retention time of PC, AF and SP were 3.119, 7.196 and 13.560 minutes respectively. Validation of the proposed method was carried out according to International Conference on Harmonisation (ICH) guidelines. Linearity range was obtained for PC, AF and SPover the concentration range of 2.1-31.5, 0.66-9.90 and 0.1-1.5 µg/mL and the r2 values were 0.9995, 0.9998 and 0.9992 respectively. The calculate limit of detection (LOD) values were 0.21, 0.066, and 0.01µg/mL and limit of quantitation (LOQ) values were 0.42, 0.132 and 0.02µg/mL for PC, AF, and SP correspondingly.
Prasada Rao Kammela,
Analytical Method Development and Validation of Simultaneous Estimation of Paracetamol, Aceclofenac and Serratiopeptidase by RP-HPLC, International Journal of Ophthalmology & Visual Science.
Vol. 2, No. 3,
2017, pp. 69-74.
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