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Cell Block Preparation for Fine Needle Aspiration Cytology of Thyroid; Cost Effective Method in a Resource Poor Setting
American Journal of Laboratory Medicine
Volume 5, Issue 6, November 2020, Pages: 167-173
Received: Nov. 4, 2020; Accepted: Nov. 27, 2020; Published: Dec. 4, 2020
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Authors
Hiroshani Hansamali Kulatunga, Department of Bio Medical Science, Kaatsu International University, Colombo, Sri Lanka
Ajith Aloka Pathirana, Department of Surgery, University of Sri Jayewardenepura, Colombo, Sri Lanka
Neluka Fernando, Department of Microbiology, University of Sri Jayewardenepura, Colombo, Sri Lanka
Bawantha Gamage, Department of Surgery, University of Sri Jayewardenepura, Colombo, Sri Lanka
Apsara Epa, Department of Radiology, Colombo South Teaching Hospital, Kalubowila, Sri Lanka
Asanga Sampath, Department of Basic Science, University of Sri Jayewardenepura, Colombo, Sri Lanka
Cherine Sosai, Department of Pathology, Colombo South Teaching Hospital, Kalubowila, Sri Lanka
Bimalka Seneviratne, Department of Pathology, University of Sri Jayewardenepura, Colombo, Sri Lanka
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Abstract
Over the past thirty years thyroid tumor incidence has triplicated and it is currently 7% of a given population. However, interestingly the thyroid cancer incidence is quite low and is approximately 5-10% of the thyroid tumor patients. Expeditious diagnosis of the thyroid nodules at the pre surgery stage is important to determine the tumor prognosis. A Widely used diagnostic approach is the direct observation of the fine needle aspiration specimens. However, using only this method many reported cases of unnecessary surgeries and re-operative incidents due to false diagnosis at the preoperative stage have been observed. Poor cellularity, loss of tissue architecture, poor cytomorphologic features in fine needle aspiration smears leads to incorrect diagnosis. Cell block technique is a better diagnostic approach at the pre-operative stage to overcome the pitfalls in direct smears as it concentrates the cells and preserve tissue architecture. Further, multiple sections with same cellularity can be prepared from a single cell block allowing the use for more advanced techniques like immunohistochemistry and molecular diagnosis. Cell block technique strengthen the diagnosis of tumors by fine needle aspiration specimens. Combined use of cell block technique with fine needle aspiration can reach to a sensitivity of about 95-100% suggesting that every FNAC specimen should be subjected to cell block preparation whenever possible.
Keywords
Cell Block, Fine Needle Aspiration Cytology, Thyroid Tumors, Immunohistochemistry
To cite this article
Hiroshani Hansamali Kulatunga, Ajith Aloka Pathirana, Neluka Fernando, Bawantha Gamage, Apsara Epa, Asanga Sampath, Cherine Sosai, Bimalka Seneviratne, Cell Block Preparation for Fine Needle Aspiration Cytology of Thyroid; Cost Effective Method in a Resource Poor Setting, American Journal of Laboratory Medicine. Vol. 5, No. 6, 2020, pp. 167-173. doi: 10.11648/j.ajlm.20200506.13
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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