Identification of Animal Muscle Proteins by 2D-Electrophoresis and Time-of-Flight Mass Spectrometry

Natalia Leonid Vostrikova; Andrew Vladimir Kulikovskii; Irina Mihail Chernuha; Andrew Nikolay Ivankin

doi:doi:10.11648/j.cbe.20170202.14

| Peer-Reviewed

Identification of Animal Muscle Proteins by 2D-Electrophoresis and Time-of-Flight Mass Spectrometry

Natalia Leonid Vostrikova, Andrew Vladimir Kulikovskii, Irina Mihail Chernuha, Andrew Nikolay Ivankin

Published in Chemical and Biomolecular Engineering (Volume 2, Issue 2)

Received: 14 January 2017 Accepted: 25 January 2017 Published: 4 March 2017

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

A method for determining the quantitative content of meat proteins by two-dimensional electrophoresis (2D). The method is designed to quantify muscle protein in raw meat samples through proteomic technology complex comprising a two-dimensional polyacrylamide gel electrophoresis and densitometry obtained electrophoregrams computer.

Published in	Chemical and Biomolecular Engineering (Volume 2, Issue 2)
DOI	10.11648/j.cbe.20170202.14
Page(s)	96-105
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Proteomics, Two-Dimensional Electrophoresis, Biomarkers, Mass Spectrometry

References

[1]	Anderson N. L., Anderson N. G. 1998. Proteome and proteomics: new technologies, new concepts, and new words. Electrophoresis, 19: 1853-1861.
[2]	Blum H., Beir H., Cross H. 1987. Improved silver staining of plant proteins, RNA and DNA inpolyacrylamide gels. Electrophoresis, 8: 126-129.
[3]	Fairbanks G., Steck T. L., Wallach D. F. 1971. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry, 10: 2607-2617.
[4]	Hoffmann K. 1997. Identification and determination of meat and foreign proteins by means of dodecylsulfat poliacrylamid gel electrophoresis. Ann. Nutr. Alim., 31: 207-215.
[5]	O'Farrell P. H. 1975. High Resolution two-dimensional analysis of proteins. J. Biol. Chem., 250: 4007-4021.
[6]	Kitchen C. M. R. 2009. Nonparametric vs parametric tests oflocation in biomedical research. American Journal of Ophthalmology, 147: 571-572.
[7]	Govorun V. M., Moszkowski S. A., Tikhonova O. V. 2003. Comparative proteomic characterization of clinical isolates of cards. Biochemistry, 68: 52-58.
[8]	Kovaleva M. A., Ivanov A. V., Kovalev L. I., Shishkin S. S., Hriapova E. E., Lisitsin A. B., Chernuha I. M., Vostrikova N. L. 2012. Proteomic technology in research of protein composition of cooked sausages. All about meat, 2: 35-42.
[9]	Kovaleva M. A., Kovalev L. I., Shishkin S. S., Ivanov A. V., Vostrikova N. L., Chernuha I. M. 2013. Proteomic study of raw meat, cooked sausages and meat products of functional. Modern problems of science and education, 5: pp. 8-12.
[10]	Chernuha I. M., Vostrikovа N. L. 2011. Coll. Materials of the 14th Intern. Scien. Conf. "Promising areas of research in the field of processing of meat raw materials and the creation of competitive foods" V. M. Gorbatov memory. Moscow, VNIIMP: 239-241.
[11]	Lisitsyn A. B., Chernukha I. M., Ivankin A. N. 2013. Comparative study of fatty acid composition of meat material from various animal species. Scientific Journal of Animal Science, 2: 124–131.
[12]	Neklyudov A. D., Ivankin A. N., Berdutina A. V. 2000. Properties and uses of protein hydrolysates. Applied Biochemistry and Microbiology, 36: 452-459.

Cite This Article

Plain Text BibTeX RIS

APA Style

Natalia Leonid Vostrikova, Andrew Vladimir Kulikovskii, Irina Mihail Chernuha, Andrew Nikolay Ivankin. (2017). Identification of Animal Muscle Proteins by 2D-Electrophoresis and Time-of-Flight Mass Spectrometry. Chemical and Biomolecular Engineering, 2(2), 96-105. https://doi.org/10.11648/j.cbe.20170202.14

Copy | Download

ACS Style

Natalia Leonid Vostrikova; Andrew Vladimir Kulikovskii; Irina Mihail Chernuha; Andrew Nikolay Ivankin. Identification of Animal Muscle Proteins by 2D-Electrophoresis and Time-of-Flight Mass Spectrometry. Chem. Biomol. Eng. 2017, 2(2), 96-105. doi: 10.11648/j.cbe.20170202.14

Copy | Download

AMA Style

Natalia Leonid Vostrikova, Andrew Vladimir Kulikovskii, Irina Mihail Chernuha, Andrew Nikolay Ivankin. Identification of Animal Muscle Proteins by 2D-Electrophoresis and Time-of-Flight Mass Spectrometry. Chem Biomol Eng. 2017;2(2):96-105. doi: 10.11648/j.cbe.20170202.14

Copy | Download

@article{10.11648/j.cbe.20170202.14,
  author = {Natalia Leonid Vostrikova and Andrew Vladimir Kulikovskii and Irina Mihail Chernuha and Andrew Nikolay Ivankin},
  title = {Identification of Animal Muscle Proteins by 2D-Electrophoresis and Time-of-Flight Mass Spectrometry},
  journal = {Chemical and Biomolecular Engineering},
  volume = {2},
  number = {2},
  pages = {96-105},
  doi = {10.11648/j.cbe.20170202.14},
  url = {https://doi.org/10.11648/j.cbe.20170202.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cbe.20170202.14},
  abstract = {A method for determining the quantitative content of meat proteins by two-dimensional electrophoresis (2D). The method is designed to quantify muscle protein in raw meat samples through proteomic technology complex comprising a two-dimensional polyacrylamide gel electrophoresis and densitometry obtained electrophoregrams computer.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Identification of Animal Muscle Proteins by 2D-Electrophoresis and Time-of-Flight Mass Spectrometry
AU  - Natalia Leonid Vostrikova
AU  - Andrew Vladimir Kulikovskii
AU  - Irina Mihail Chernuha
AU  - Andrew Nikolay Ivankin
Y1  - 2017/03/04
PY  - 2017
N1  - https://doi.org/10.11648/j.cbe.20170202.14
DO  - 10.11648/j.cbe.20170202.14
T2  - Chemical and Biomolecular Engineering
JF  - Chemical and Biomolecular Engineering
JO  - Chemical and Biomolecular Engineering
SP  - 96
EP  - 105
PB  - Science Publishing Group
SN  - 2578-8884
UR  - https://doi.org/10.11648/j.cbe.20170202.14
AB  - A method for determining the quantitative content of meat proteins by two-dimensional electrophoresis (2D). The method is designed to quantify muscle protein in raw meat samples through proteomic technology complex comprising a two-dimensional polyacrylamide gel electrophoresis and densitometry obtained electrophoregrams computer.
VL  - 2
IS  - 2
ER  -

Copy | Download

Author Information

Natalia Leonid Vostrikova

Department of Analytical Research, the V.M. Gorbatov All-Russian Meat Research Institute, Moscow, Russia
Andrew Vladimir Kulikovskii

Department of Analytical Research, the V.M. Gorbatov All-Russian Meat Research Institute, Moscow, Russia
Irina Mihail Chernuha

Department of Analytical Research, the V.M. Gorbatov All-Russian Meat Research Institute, Moscow, Russia
Andrew Nikolay Ivankin

Department of Chemistry, Bauman Moscow State Technical University, Moscow, Russia

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Natalia Leonid Vostrikova, Andrew Vladimir Kulikovskii, Irina Mihail Chernuha, Andrew Nikolay Ivankin. (2017). Identification of Animal Muscle Proteins by 2D-Electrophoresis and Time-of-Flight Mass Spectrometry. Chemical and Biomolecular Engineering, 2(2), 96-105. https://doi.org/10.11648/j.cbe.20170202.14

Copy | Download

ACS Style

Natalia Leonid Vostrikova; Andrew Vladimir Kulikovskii; Irina Mihail Chernuha; Andrew Nikolay Ivankin. Identification of Animal Muscle Proteins by 2D-Electrophoresis and Time-of-Flight Mass Spectrometry. Chem. Biomol. Eng. 2017, 2(2), 96-105. doi: 10.11648/j.cbe.20170202.14

Copy | Download

AMA Style

Natalia Leonid Vostrikova, Andrew Vladimir Kulikovskii, Irina Mihail Chernuha, Andrew Nikolay Ivankin. Identification of Animal Muscle Proteins by 2D-Electrophoresis and Time-of-Flight Mass Spectrometry. Chem Biomol Eng. 2017;2(2):96-105. doi: 10.11648/j.cbe.20170202.14

Copy | Download

@article{10.11648/j.cbe.20170202.14,
  author = {Natalia Leonid Vostrikova and Andrew Vladimir Kulikovskii and Irina Mihail Chernuha and Andrew Nikolay Ivankin},
  title = {Identification of Animal Muscle Proteins by 2D-Electrophoresis and Time-of-Flight Mass Spectrometry},
  journal = {Chemical and Biomolecular Engineering},
  volume = {2},
  number = {2},
  pages = {96-105},
  doi = {10.11648/j.cbe.20170202.14},
  url = {https://doi.org/10.11648/j.cbe.20170202.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cbe.20170202.14},
  abstract = {A method for determining the quantitative content of meat proteins by two-dimensional electrophoresis (2D). The method is designed to quantify muscle protein in raw meat samples through proteomic technology complex comprising a two-dimensional polyacrylamide gel electrophoresis and densitometry obtained electrophoregrams computer.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Identification of Animal Muscle Proteins by 2D-Electrophoresis and Time-of-Flight Mass Spectrometry
AU  - Natalia Leonid Vostrikova
AU  - Andrew Vladimir Kulikovskii
AU  - Irina Mihail Chernuha
AU  - Andrew Nikolay Ivankin
Y1  - 2017/03/04
PY  - 2017
N1  - https://doi.org/10.11648/j.cbe.20170202.14
DO  - 10.11648/j.cbe.20170202.14
T2  - Chemical and Biomolecular Engineering
JF  - Chemical and Biomolecular Engineering
JO  - Chemical and Biomolecular Engineering
SP  - 96
EP  - 105
PB  - Science Publishing Group
SN  - 2578-8884
UR  - https://doi.org/10.11648/j.cbe.20170202.14
AB  - A method for determining the quantitative content of meat proteins by two-dimensional electrophoresis (2D). The method is designed to quantify muscle protein in raw meat samples through proteomic technology complex comprising a two-dimensional polyacrylamide gel electrophoresis and densitometry obtained electrophoregrams computer.
VL  - 2
IS  - 2
ER  -

Copy | Download