Screening for Hydrocarbon Degrading Bacteria Using Redox Indicator 2, 6-Dichlorophenol Indophenol

James Iniobong Ime; Ibuot Aniefon Alphonsus; Akpan Patience Saturday; Ben Mayen Godwin; Etuk Christiana Utibe; Umoren Emmanuel Anthony

doi:doi:10.11648/j.cbe.20180302.11

Screening for Hydrocarbon Degrading Bacteria Using Redox Indicator 2, 6-Dichlorophenol Indophenol

James Iniobong Ime, Ibuot Aniefon Alphonsus, Akpan Patience Saturday, Ben Mayen Godwin, Etuk Christiana Utibe, Umoren Emmanuel Anthony

Published in Chemical and Biomolecular Engineering (Volume 3, Issue 2)

Received: 2 August 2018 Accepted: 7 September 2018 Published: 13 October 2018

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Abstract

Contamination by petroleum products and its derivatives promotes serious environmental damage. Biodegradation capacity studies are important when deciding the correct bioremediation strategy to employ. The use of redox indicator 2, 6-Dichlorophenol Indophenol (DCPIP) is a rapid, simple and low cost model for evaluating capability of microorganisms to utilize and/or degrade petroleum hydrocarbons. This study involved isolation and screening of bacterial species capable of utilizing hydrocarbons from soil at two auto-mechanic workshops in Uyo, Akwa Ibom State. Results of the physicochemical analysis of the soil samples showed higher levels of properties (Moisture content, Organic Carbon content, Total Hydrocarbon Content) in the polluted soil samples when compared with unpolluted (control) soil sample. Total heterotrophic bacterial populations in polluted soil samples ranged between 4.4±1.90x10⁷ and 6.0±32.0x10⁷ CFU/g while hydrocarbon utilizing bacterial counts were between 3.2±0.05x10⁷ and 5.2±25.2x10⁷ CFU/g. Eight bacteria species capable of utilizing petroleum were isolated from these soils by enrichment technique. Isolated bacteria include: Corynebacterium spp, Pseudomonas aeruginosa, Micrococcus agilis, Flavobacterium aquatile, Staphylococcus aureus, Micrococcus luteus, Serratia odorifera and Bacillus substilis. Screening of bacterial isolates for efficiency in hydrocarbon utilization using DCPIP, measured by using colorimetry, revealed Bacillus substilis, Pseudomonas aeruginosa, Micrococccus agilis, Flavobacterium aquatile Micrococcus luteus and Corynebacterium spp to be able to rapidly utilize hydrocarbons from waste engine oil.

Published in	Chemical and Biomolecular Engineering (Volume 3, Issue 2)
DOI	10.11648/j.cbe.20180302.11
Page(s)	11-16
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Hydrocarbon, Bacteria, 2, 6-Dichlorophenol Indophenols

References

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James Iniobong Ime, Ibuot Aniefon Alphonsus, Akpan Patience Saturday, Ben Mayen Godwin, Etuk Christiana Utibe, et al. (2018). Screening for Hydrocarbon Degrading Bacteria Using Redox Indicator 2, 6-Dichlorophenol Indophenol. Chemical and Biomolecular Engineering, 3(2), 11-16. https://doi.org/10.11648/j.cbe.20180302.11

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ACS Style

James Iniobong Ime; Ibuot Aniefon Alphonsus; Akpan Patience Saturday; Ben Mayen Godwin; Etuk Christiana Utibe, et al. Screening for Hydrocarbon Degrading Bacteria Using Redox Indicator 2, 6-Dichlorophenol Indophenol. Chem. Biomol. Eng. 2018, 3(2), 11-16. doi: 10.11648/j.cbe.20180302.11

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AMA Style

James Iniobong Ime, Ibuot Aniefon Alphonsus, Akpan Patience Saturday, Ben Mayen Godwin, Etuk Christiana Utibe, et al. Screening for Hydrocarbon Degrading Bacteria Using Redox Indicator 2, 6-Dichlorophenol Indophenol. Chem Biomol Eng. 2018;3(2):11-16. doi: 10.11648/j.cbe.20180302.11

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@article{10.11648/j.cbe.20180302.11,
  author = {James Iniobong Ime and Ibuot Aniefon Alphonsus and Akpan Patience Saturday and Ben Mayen Godwin and Etuk Christiana Utibe and Umoren Emmanuel Anthony},
  title = {Screening for Hydrocarbon Degrading Bacteria Using Redox Indicator 2, 6-Dichlorophenol Indophenol},
  journal = {Chemical and Biomolecular Engineering},
  volume = {3},
  number = {2},
  pages = {11-16},
  doi = {10.11648/j.cbe.20180302.11},
  url = {https://doi.org/10.11648/j.cbe.20180302.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cbe.20180302.11},
  abstract = {Contamination by petroleum products and its derivatives promotes serious environmental damage. Biodegradation capacity studies are important when deciding the correct bioremediation strategy to employ. The use of redox indicator 2, 6-Dichlorophenol Indophenol (DCPIP) is a rapid, simple and low cost model for evaluating capability of microorganisms to utilize and/or degrade petroleum hydrocarbons. This study involved isolation and screening of bacterial species capable of utilizing hydrocarbons from soil at two auto-mechanic workshops in Uyo, Akwa Ibom State. Results of the physicochemical analysis of the soil samples showed higher levels of properties (Moisture content, Organic Carbon content, Total Hydrocarbon Content) in the polluted soil samples when compared with unpolluted (control) soil sample. Total heterotrophic bacterial populations in polluted soil samples ranged between 4.4±1.90x107 and 6.0±32.0x107 CFU/g while hydrocarbon utilizing bacterial counts were between 3.2±0.05x107 and 5.2±25.2x107 CFU/g. Eight bacteria species capable of utilizing petroleum were isolated from these soils by enrichment technique. Isolated bacteria include: Corynebacterium spp, Pseudomonas aeruginosa, Micrococcus agilis, Flavobacterium aquatile, Staphylococcus aureus, Micrococcus luteus, Serratia odorifera and Bacillus substilis. Screening of bacterial isolates for efficiency in hydrocarbon utilization using DCPIP, measured by using colorimetry, revealed Bacillus substilis, Pseudomonas aeruginosa, Micrococccus agilis, Flavobacterium aquatile Micrococcus luteus and Corynebacterium spp to be able to rapidly utilize hydrocarbons from waste engine oil.},
 year = {2018}
}

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TY  - JOUR
T1  - Screening for Hydrocarbon Degrading Bacteria Using Redox Indicator 2, 6-Dichlorophenol Indophenol
AU  - James Iniobong Ime
AU  - Ibuot Aniefon Alphonsus
AU  - Akpan Patience Saturday
AU  - Ben Mayen Godwin
AU  - Etuk Christiana Utibe
AU  - Umoren Emmanuel Anthony
Y1  - 2018/10/13
PY  - 2018
N1  - https://doi.org/10.11648/j.cbe.20180302.11
DO  - 10.11648/j.cbe.20180302.11
T2  - Chemical and Biomolecular Engineering
JF  - Chemical and Biomolecular Engineering
JO  - Chemical and Biomolecular Engineering
SP  - 11
EP  - 16
PB  - Science Publishing Group
SN  - 2578-8884
UR  - https://doi.org/10.11648/j.cbe.20180302.11
AB  - Contamination by petroleum products and its derivatives promotes serious environmental damage. Biodegradation capacity studies are important when deciding the correct bioremediation strategy to employ. The use of redox indicator 2, 6-Dichlorophenol Indophenol (DCPIP) is a rapid, simple and low cost model for evaluating capability of microorganisms to utilize and/or degrade petroleum hydrocarbons. This study involved isolation and screening of bacterial species capable of utilizing hydrocarbons from soil at two auto-mechanic workshops in Uyo, Akwa Ibom State. Results of the physicochemical analysis of the soil samples showed higher levels of properties (Moisture content, Organic Carbon content, Total Hydrocarbon Content) in the polluted soil samples when compared with unpolluted (control) soil sample. Total heterotrophic bacterial populations in polluted soil samples ranged between 4.4±1.90x107 and 6.0±32.0x107 CFU/g while hydrocarbon utilizing bacterial counts were between 3.2±0.05x107 and 5.2±25.2x107 CFU/g. Eight bacteria species capable of utilizing petroleum were isolated from these soils by enrichment technique. Isolated bacteria include: Corynebacterium spp, Pseudomonas aeruginosa, Micrococcus agilis, Flavobacterium aquatile, Staphylococcus aureus, Micrococcus luteus, Serratia odorifera and Bacillus substilis. Screening of bacterial isolates for efficiency in hydrocarbon utilization using DCPIP, measured by using colorimetry, revealed Bacillus substilis, Pseudomonas aeruginosa, Micrococccus agilis, Flavobacterium aquatile Micrococcus luteus and Corynebacterium spp to be able to rapidly utilize hydrocarbons from waste engine oil.
VL  - 3
IS  - 2
ER  -

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Author Information

James Iniobong Ime

Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria
Ibuot Aniefon Alphonsus

Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria
Akpan Patience Saturday

Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria
Ben Mayen Godwin

Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria
Etuk Christiana Utibe

Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria
Umoren Emmanuel Anthony

Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria

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APA Style

James Iniobong Ime, Ibuot Aniefon Alphonsus, Akpan Patience Saturday, Ben Mayen Godwin, Etuk Christiana Utibe, et al. (2018). Screening for Hydrocarbon Degrading Bacteria Using Redox Indicator 2, 6-Dichlorophenol Indophenol. Chemical and Biomolecular Engineering, 3(2), 11-16. https://doi.org/10.11648/j.cbe.20180302.11

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ACS Style

James Iniobong Ime; Ibuot Aniefon Alphonsus; Akpan Patience Saturday; Ben Mayen Godwin; Etuk Christiana Utibe, et al. Screening for Hydrocarbon Degrading Bacteria Using Redox Indicator 2, 6-Dichlorophenol Indophenol. Chem. Biomol. Eng. 2018, 3(2), 11-16. doi: 10.11648/j.cbe.20180302.11

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AMA Style

James Iniobong Ime, Ibuot Aniefon Alphonsus, Akpan Patience Saturday, Ben Mayen Godwin, Etuk Christiana Utibe, et al. Screening for Hydrocarbon Degrading Bacteria Using Redox Indicator 2, 6-Dichlorophenol Indophenol. Chem Biomol Eng. 2018;3(2):11-16. doi: 10.11648/j.cbe.20180302.11

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@article{10.11648/j.cbe.20180302.11,
  author = {James Iniobong Ime and Ibuot Aniefon Alphonsus and Akpan Patience Saturday and Ben Mayen Godwin and Etuk Christiana Utibe and Umoren Emmanuel Anthony},
  title = {Screening for Hydrocarbon Degrading Bacteria Using Redox Indicator 2, 6-Dichlorophenol Indophenol},
  journal = {Chemical and Biomolecular Engineering},
  volume = {3},
  number = {2},
  pages = {11-16},
  doi = {10.11648/j.cbe.20180302.11},
  url = {https://doi.org/10.11648/j.cbe.20180302.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cbe.20180302.11},
  abstract = {Contamination by petroleum products and its derivatives promotes serious environmental damage. Biodegradation capacity studies are important when deciding the correct bioremediation strategy to employ. The use of redox indicator 2, 6-Dichlorophenol Indophenol (DCPIP) is a rapid, simple and low cost model for evaluating capability of microorganisms to utilize and/or degrade petroleum hydrocarbons. This study involved isolation and screening of bacterial species capable of utilizing hydrocarbons from soil at two auto-mechanic workshops in Uyo, Akwa Ibom State. Results of the physicochemical analysis of the soil samples showed higher levels of properties (Moisture content, Organic Carbon content, Total Hydrocarbon Content) in the polluted soil samples when compared with unpolluted (control) soil sample. Total heterotrophic bacterial populations in polluted soil samples ranged between 4.4±1.90x107 and 6.0±32.0x107 CFU/g while hydrocarbon utilizing bacterial counts were between 3.2±0.05x107 and 5.2±25.2x107 CFU/g. Eight bacteria species capable of utilizing petroleum were isolated from these soils by enrichment technique. Isolated bacteria include: Corynebacterium spp, Pseudomonas aeruginosa, Micrococcus agilis, Flavobacterium aquatile, Staphylococcus aureus, Micrococcus luteus, Serratia odorifera and Bacillus substilis. Screening of bacterial isolates for efficiency in hydrocarbon utilization using DCPIP, measured by using colorimetry, revealed Bacillus substilis, Pseudomonas aeruginosa, Micrococccus agilis, Flavobacterium aquatile Micrococcus luteus and Corynebacterium spp to be able to rapidly utilize hydrocarbons from waste engine oil.},
 year = {2018}
}

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TY  - JOUR
T1  - Screening for Hydrocarbon Degrading Bacteria Using Redox Indicator 2, 6-Dichlorophenol Indophenol
AU  - James Iniobong Ime
AU  - Ibuot Aniefon Alphonsus
AU  - Akpan Patience Saturday
AU  - Ben Mayen Godwin
AU  - Etuk Christiana Utibe
AU  - Umoren Emmanuel Anthony
Y1  - 2018/10/13
PY  - 2018
N1  - https://doi.org/10.11648/j.cbe.20180302.11
DO  - 10.11648/j.cbe.20180302.11
T2  - Chemical and Biomolecular Engineering
JF  - Chemical and Biomolecular Engineering
JO  - Chemical and Biomolecular Engineering
SP  - 11
EP  - 16
PB  - Science Publishing Group
SN  - 2578-8884
UR  - https://doi.org/10.11648/j.cbe.20180302.11
AB  - Contamination by petroleum products and its derivatives promotes serious environmental damage. Biodegradation capacity studies are important when deciding the correct bioremediation strategy to employ. The use of redox indicator 2, 6-Dichlorophenol Indophenol (DCPIP) is a rapid, simple and low cost model for evaluating capability of microorganisms to utilize and/or degrade petroleum hydrocarbons. This study involved isolation and screening of bacterial species capable of utilizing hydrocarbons from soil at two auto-mechanic workshops in Uyo, Akwa Ibom State. Results of the physicochemical analysis of the soil samples showed higher levels of properties (Moisture content, Organic Carbon content, Total Hydrocarbon Content) in the polluted soil samples when compared with unpolluted (control) soil sample. Total heterotrophic bacterial populations in polluted soil samples ranged between 4.4±1.90x107 and 6.0±32.0x107 CFU/g while hydrocarbon utilizing bacterial counts were between 3.2±0.05x107 and 5.2±25.2x107 CFU/g. Eight bacteria species capable of utilizing petroleum were isolated from these soils by enrichment technique. Isolated bacteria include: Corynebacterium spp, Pseudomonas aeruginosa, Micrococcus agilis, Flavobacterium aquatile, Staphylococcus aureus, Micrococcus luteus, Serratia odorifera and Bacillus substilis. Screening of bacterial isolates for efficiency in hydrocarbon utilization using DCPIP, measured by using colorimetry, revealed Bacillus substilis, Pseudomonas aeruginosa, Micrococccus agilis, Flavobacterium aquatile Micrococcus luteus and Corynebacterium spp to be able to rapidly utilize hydrocarbons from waste engine oil.
VL  - 3
IS  - 2
ER  -

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