Hepatoprotective Potentiality of Various Fractions of Ethanolic Extracts of Lawsonia Inermis (Henna) Leaves Against Chemical-Induced Hepatitis in Rats
Biochemistry and Molecular Biology
Volume 1, Issue 2, September 2016, Pages: 17-22
Received: Jul. 25, 2016; Accepted: Aug. 2, 2016; Published: Aug. 21, 2016
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Authors
Khondakar Mahmudul Hasan, Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh
Samina Yesmin, Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh
Sarkar Farhana Akhter, Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh
Subrata Paul, Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh
Sabarni Sarker, Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh
Md Ariful Islam, Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh;Department of Organ Anatomy, Tohoku University, Tohoku, Japan
Mir Imam Ibne Wahed, Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh
Md Rafiqul Islam Khan, Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh
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Abstract
Liver is a metabolically active organ responsible for many vital life functions. More than 900 drugs, toxins, and herbs have been reported to cause liver injury. In spite of tremendous advances in modern medicine, there are hardly any reliable drugs that protect the liver from damage and/or help in regeneration of hepatic cell. It is, therefore, necessary to search for effective and safe herbal drugs for the treatment of liver disease. In the present study, we investigated the hepatoprotective activity of ethanolic extracts of various fractions (Ethyl acetate, Petroleum ether and Chloroform) of Lowsonia inermis leaves in carbon tetrachloride (CCl4)-induced hepatitis rats. Hepatotoxicity was induced in rats by intraperitoneal injection of CCl4 on alternate days for 7 days. After 7 days of pretreatment of test extracts, the biochemical markers such as Serum bilirubin, Serum Glutamic Pyruvic Transaminase (SGPT), Serum Glutamic Oxaloacetic Transaminase (SGOT) and Alkaline Phosphatase (ALP), were examined. The extracts at the doses of 200 mg/kg p.o. significantly (P < 0.05) reduced the elevated levels of Serum bilirubin, SGPT, SGOT and SALP compared to the CCl4-treated group alone. The findings of the study indicate that the leaf extract of L. inermis showed a potential hepatoprotective activity and the protective action might be manifested by reducing the oxidative stress as the leaf extracts possess flavonoid and phenolic compounds which have free radical scavenging and antioxidant activity. However, further study is necessary uncovering the exact molecular mechanism of hepatoprotective activity.
Keywords
Antioxidant Activity, Carbon Tetrachloride, Ethanolic Extracts, Hepatoprotective Activity
To cite this article
Khondakar Mahmudul Hasan, Samina Yesmin, Sarkar Farhana Akhter, Subrata Paul, Sabarni Sarker, Md Ariful Islam, Mir Imam Ibne Wahed, Md Rafiqul Islam Khan, Hepatoprotective Potentiality of Various Fractions of Ethanolic Extracts of Lawsonia Inermis (Henna) Leaves Against Chemical-Induced Hepatitis in Rats, Biochemistry and Molecular Biology. Vol. 1, No. 2, 2016, pp. 17-22. doi: 10.11648/j.bmb.20160102.12
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Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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