Biochemistry and Molecular Biology
Volume 2, Issue 6, November 2017, Pages: 120-130
Received: Oct. 30, 2017;
Accepted: Nov. 11, 2017;
Published: Dec. 11, 2017
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Mahendra Kumar Trivedi, Trivedi Global, Inc., Henderson, Nevada, USA
Alice Branton, Trivedi Global, Inc., Henderson, Nevada, USA
Dahryn Trivedi, Trivedi Global, Inc., Henderson, Nevada, USA
Gopal Nayak, Trivedi Global, Inc., Henderson, Nevada, USA
Ariadne Esmene Afaganis, Trivedi Global, Inc., Henderson, Nevada, USA
Barbara Marie Bader, Trivedi Global, Inc., Henderson, Nevada, USA
Brian A. Weekes, Trivedi Global, Inc., Henderson, Nevada, USA
Daphne Luisa Dumas, Trivedi Global, Inc., Henderson, Nevada, USA
Denise Marie Fiedler, Trivedi Global, Inc., Henderson, Nevada, USA
Dennille Mellesia Smith, Trivedi Global, Inc., Henderson, Nevada, USA
Desi Pano, Trivedi Global, Inc., Henderson, Nevada, USA
Donna Felice Galla, Trivedi Global, Inc., Henderson, Nevada, USA
Donna Maria Alija, Trivedi Global, Inc., Henderson, Nevada, USA
Elaine Barbara Mullins, Trivedi Global, Inc., Henderson, Nevada, USA
Elaine M. Scorza, Trivedi Global, Inc., Henderson, Nevada, USA
Ellia O'Donnell, Trivedi Global, Inc., Henderson, Nevada, USA
Fabio Massimo Paciucci, Trivedi Global, Inc., Henderson, Nevada, USA
Frances Goodman Warlick, Trivedi Global, Inc., Henderson, Nevada, USA
Haddon Norman Salt, Trivedi Global, Inc., Henderson, Nevada, USA
Inthirani Arul, Trivedi Global, Inc., Henderson, Nevada, USA
Jacqueline Y. Andrews, Trivedi Global, Inc., Henderson, Nevada, USA
James Jay McLeran, Trivedi Global, Inc., Henderson, Nevada, USA
James Stephen Burnett, Trivedi Global, Inc., Henderson, Nevada, USA
Jean Caroline White, Trivedi Global, Inc., Henderson, Nevada, USA
Mayank Gangwar, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
A new proprietary herbomineral formulation was formulated, consisting of essential ingredients viz. herbal root extract of ashwagandha and minerals (zinc, magnesium, and selenium). The present study aimed to evaluate the impact of The Trivedi Effect®- Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) based herbomineral formulation in male Sprague Dawley (SD) rats for immune biomarkers modulation. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Treated sample, which received the Biofield Energy Healing Treatment remotely from twenty renowned Biofield Energy Healers. The immunomodulatory potential was studied in male SD rats using various immune biomarkers such as IgG and IgM, CD4+ and CD8+, hematology, lipid profile, hepatic enzymes, sex hormone, and antioxidant profile. The humoral immune response showed an increase IgG level by 6.10% in the Biofield Energy Treated test formulation (G4) group compared with the disease control (G2) group. Cellular immune response i.e. CD4+ and CD8+ counts were significantly improved by 51.64% and 52.09%, respectively in G4 compared with the G2 group. The lymphocytes level were increased by 38.87%, while TLC, neutrophils, eosinophils, and monocytes count were decreased by 7.38%, 19.35%, 21.14%, and 3.43%, respectively in G4 compared with the G2 group. Lipid analysis showed that triglycerides and VLDL levels were decreased by 6.02% and 5.97%, respectively while HDL level was significantly increased by 28.44% in G4 compared with the G2 group. Hepatic biomarkers viz. SGOT, CK-MB, total protein, total bilirubin, and globulin by 6.27%, 14.97%, 2.85%, 16.66%, and 5.90%, respectively in G4 compared with the G2 group. However, the testosterone level was significantly increased in G4 and untreated test formulation (G5) groups by 135.74% and 28.09%, respectively compared with the G2 group. Additionally, the antioxidant assay showed that the lipid peroxidation (LPO) level was decreased, while superoxide dismutase (SOD) and catalase levels were increased by 59.72% and 20.07%, respectively in the G4 compared with the G2 group. Overall, the study results suggested that the Biofield Energy Treated test formulation showed an improved cellular and humoral immune responses along with improved hematological and biochemical animal profile compared with the untreated test formulation. Consequently, it can be established that The Trivedi Effect®-Biofield Energy Healing has the significant capacity for immunomodulatory effect, which may also be useful in organ transplants, anti-aging, and stress management by improving overall health and quality of life.
Mahendra Kumar Trivedi,
Ariadne Esmene Afaganis,
Barbara Marie Bader,
Brian A. Weekes,
Daphne Luisa Dumas,
Denise Marie Fiedler,
Dennille Mellesia Smith,
Donna Felice Galla,
Donna Maria Alija,
Elaine Barbara Mullins,
Elaine M. Scorza,
Fabio Massimo Paciucci,
Frances Goodman Warlick,
Haddon Norman Salt,
Jacqueline Y. Andrews,
James Jay McLeran,
James Stephen Burnett,
Jean Caroline White,
Differential Regulation of Immune Biomarkers by the Trivedi Effect®- Energy of Consciousness Healing Treatment Based Herbomineral Formulation in Male Sprague Dawley Rats, Biochemistry and Molecular Biology.
Vol. 2, No. 6,
2017, pp. 120-130.
Simon R (2011) Genomic biomarkers in predictive medicine: An interim analysis. EMBO Mol Med 3: 429-435.
Janeway CA Jr (2001) How the immune system protects the host from infection. Microbes Infect 3: 1167-1171.
William JE (2001) Review of antiviral and immunomodulatory properties of plants of the Peruvian rainforest. Alter Med Rev 6: 567-579.
Mahima, Rahal A, Deb R, Latheef SK, Abdul Samad H, Tiwari R, Verma AK, Kumar A, Dhama K (2012) Immunomodulatory and therapeutic potentials of herbal, traditional/indigenous and ethnoveterinary medicines. Pak J Biol Sci 15: 754-774.
Sharma ML, Rao CS, Duda PL (1994) Immunostimulatory activity of Picrorhiza kurroa leaf extract. J Ethnopharmacol 41: 185-192.
Wang JZ, Mao XJ, Ito H, Shimura K (1991) Immunomodulatory activity of polysaccharide from Acanthopanex obovatus roots. Planta Med 57: 335-336
Karley D, Gupta D, Tiwari A (2011) Biomarkers: The future of medical science to detect cancer. J Mol Biomark Diagn 2: 118.
Lukác N, Massanyi P (2007) Effects of trace elements on the immune system. Epidemiol Mikrobiol Imunol 56: 3-9.
Galland L (1998) Magnesium and immune function: An overview. Magnesium 7: 290-299.
Wintergerst ES, Maggini S, Hornig DH (2007) Contribution of selected vitamins and trace elements to immune function. Ann Nutr Metab 51: 301-323.
Ziauddin M, Phansalkar N, Patki P, Diwanay S, Patwardhan B (1996) Studies on the immunomodulatory effects of ashwagandha. J Ethnopharmacol 50: 69-76.
Singh N, Bhalla M, de Jager P, Gilca M (2011) An overview on ashwagandha: A rasayana (rejuvenator) of Ayurveda. Afr J Tradit Complement Altern Med 8: 208-213.
James SJ, Swenseid M, Makinodan T (1987) Macrophage-mediated depression of T-cell proliferation in zinc-deficient mice. J Nutrition 117: 1982.
Engle TE, Nockels DF, Kimberling CV, Weaber DL, Johnson AB (1997) Zinc repletion with organic and inorganic forms of zinc and protein turnover in marginally zinc-deficient calves. J Anim Sci 75: 3074.
Salimian J, Arefpour MA, Riazipour M, Poursasan N (2004) Immunomodulatory effects of selenium and vitamin E on alterations in T lymphocyte subsets induced by T-2 toxin. Immunopharmacol Immunotoxicol 36: 275-281.
Sugimoto J, Romani AM, Valentin-Torres AM, Luciano AA, Ramirez Kitchen CM, Funderburg N, Mesiano S, Bernstein HB (2012) Magnesium decreases inflammatory cytokine production: A novel innate immunomodulatory mechanism. J Immunol 188: 6338-6346.
Rubik B (1994) Manual healing methods. Alternative medicine: expanding medical horizons, Washington, DC, US Government Printing Office, NIH Publication No. 94-66.
Cooper EL (2007) The immune system and complementary and alternative medicine. Evid Based Complement Alternat Med 4: 5-8.
Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) In vitro evaluation of biofield treatment on viral load against human immunodeficiency-1 and cytomegalo viruses. American Journal of Health Research 3: 338-343.
Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) In vitro Evaluation of biofield treatment on Enterobacter cloacae: Impact on antimicrobial susceptibility and biotype. J Bacteriol Parasitol 6: 241.
Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) Evaluation of Biofield Modality on Viral Load of Hepatitis B and C Viruses. J Antivir Antiretrovir 7: 83-88.
Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) An impact of biofield treatment: Antimycobacterial susceptibility potential using BACTEC 460/MGIT-TB system. Mycobact Dis 5: 189.
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of antibiogram, genotype and phylogenetic analysis of biofield treated Nocardia otitidis. Biol Syst Open Access 4: 143.
Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015) Antibiogram, biochemical reactions, and genotypic pattern of biofield treated Pseudomonas aeruginosa. J Trop Dis 4: 181.
Trivedi MK, Patil S, Tallapragada RM (2013) Effect of bio field treatment on the physical and thermal characteristics of vanadium pentoxide powders. J Material Sci Eng S 11: 001.
Trivedi MK, Branton A, Trivedi D, Shettigar H, Bairwa K, Jana S (2015) Fourier transform infrared and ultraviolet-visible spectroscopic characterization of biofield treated salicylic acid and sparfloxacin. Nat Prod Chem Res 3: 186.
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Potential impact of biofield treatment on atomic and physical characteristics of magnesium. Vitam Miner 3: 129.
Trivedi MK, Branton A, Trivedi D, Nayak G, Sethi KK, Jana S (2016) Gas chromatography-mass spectrometry based isotopic abundance ratio analysis of biofield energy treated methyl-2-napthylether (Nerolin). American Journal of Physical Chemistry 5: 80-86.
Trivedi MK, Branton A, Trivedi D, Nayak G, Panda P, Jana S (2016) Gas chromatography-mass spectrometric analysis of isotopic abundance of 13C, 2H, and 18O in biofield energy treated p-tertiary butylphenol (PTBP). American Journal of Chemical Engineering 4: 78-86.
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of biochemical marker - glutathione and DNA fingerprinting of biofield energy treated Oryza sativa. American Journal of BioScience 3: 243-248.
Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2016) Molecular analysis of biofield treated eggplant and watermelon crops. Adv Crop Sci Tech 4: 208.
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Physical, atomic and thermal properties of biofield treated lithium powder. J Adv Chem Eng 5: 136.
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Evaluation of biofield energy treatment on physical and thermal characteristics of selenium powder. Journal of Food and Nutrition Sciences 3: 223-228.
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Mishra RK, Latiyal O, Jana S (2015) Physicochemical characterization of biofield energy treated calcium carbonate powder. American Journal of Health Research 3: 368-375.
Ladics GS (2007) Primary immune response to sheep red blood cells (SRBC) as the conventional T-cell dependent antibody response (TDAR) test. J Immunotoxicol 4: 149-152.
King EJ, Armstrong AR (1934) Estimation of alkaline phosphatase. Canad Med Assoc J 311: 152-156.
Folch J, Lees M, Sloane Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissue. J Biol Chem 226: 497-509.
Devasagayam TPA, Tarachand U (1987) Decreased lipid peroxidation in the rat kidney during gestation. Biochem Biophys Res Commun 145: 134-138.
Marklund S, Marklund G (1974) Involvement of superoxide anion radical in the autooxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47: 469-474.
Sinha AK (1972) Colorimetric assay of catalase. Anal Biochem 47: 389-394.
Malik F, Singh J, Khajuria A, Suri KA, Satti NK, Singh S, Kaul MK, Kumar A, Bhatia A, Qazi GN (2007) A standardized root extract of Withania somnifera and its major constituent withanolide-A elicit humoral and cell-mediated immune responses by up regulation of Th1-dominant polarization in BALB/c mice. Life Sci 80: 1525-1538.
Spallholz JE, Stewart JR (1989) Advances in the role of minerals in immunobiology. Biol Trace Elem Res 19: 129-151.
Uppal SS, Verma S, Dhot PS (2003) Normal values of CD4 and CD8 lymphocyte subsets in healthy indian adults and the effects of sex, age, ethnicity, and smoking. Cytometry B Clin Cytom 52: 32-36.
Mikolai J, Erlandsen A, Murison A, Brown KA, Gregory WL, Raman-Caplan P, Zwickey HL (2008) In vivo effects of ashwagandha (Withania somnifera) extract on the activation of lymphocytes. J Altern Complement Med 15: 423-430.
Miceli MC, Parnes JR (1991) The roles of CD4 and CD8 in T cell activation. Semin Immunol 3: 133-141.
Balakrishnan K, Adams LE (1995) The role of the lymphocyte in an immune response. Immunol Invest 24: 233-244.
Andallu B, Radhika B (2000) Hypoglycemic, diuretic and hypocholesterolemic effect of winter cherry (Withania somnifera, Dunal) root. Indian J Exp Biol 38: 607-609.
Bunglavan SJ, Garg AK, Dass RS, Shrivastava S (2014) Effect of supplementation of different levels of selenium as nanoparticles/sodium selenite on blood biochemical profile and humoral immunity in male wistar rats. Vet World 7: 1075-1081.
Fox C, Ramsoomair D, Carter C (2001) Magnesium: its proven and potential clinical significance. South Med J 94: 1195-1201.
Payahoo L, Ostadrahimi A, Mobasseri M, Bishak YK, Farrin N, Jafarabadi MA, Mahluji S (2013) Effects of zinc supplementation on the anthropometric measurements, lipid profiles and fasting blood glucose in the healthy obese adults. Adv Pharm Bull 3: 161-165.
Torres-Duran PV, Ferreira-Hermosillo A, Juarez-Oropeza MA, Elias-Viñas D, Verdugo-Diaz L (2007) Effects of whole body exposure to extremely low frequency electromagnetic fields (ELF-EMF) on serum and liver lipid levels, in the rat. Lipids Health Dis 6: 31.
Giannini EG, Testa R, Savarino V (2005) Liver enzyme alteration: a guide for clinicians. CMAJ 172: 367-379.
Sidhu P, Garg ML, Dhawan DK (2005) Protective effects of zinc on oxidative stress enzymes in liver of protein-deficient rats. Drug Chem Toxicol 28: 211-230.
El-Boshy ME, Risha EF, Abdelhamid FM, Mubarak MS, Hadda TB (2015) Protective effects of selenium against cadmium induced hematological disturbances, immunosuppressive, oxidative stress and hepatorenal damage in rats. J Trace Elem Med Biol 29: 104-110.
Karandish M, Tamimi M, Shayesteh AA, Haghighizadeh MH, Jalali MT (2013) The effect of magnesium supplementation and weight loss on liver enzymes in patients with nonalcoholic fatty liver disease. J Res Med Sci 18: 573-579.
Sabiba EP, Rasool M, Vedi M, Navaneethan D, Ravichander M, Parthasarathy P, Thella SR (2013) Hepatoprotective and antioxidant potential of Withania somnifera against paracetamol-induced liver damage in rats. Int J Pharm Pharm Sci 5: 648-651.
Ambiye VR, Langade D, Dongre S, Aptikar P, Kulkarni M, Dongre A (2013) Clinical Evaluation of the spermatogenic activity of the root extract of ashwagandha (Withania somnifera) in oligospermic males: A pilot study. Evidence-based Complementary and Alternative Medicine : eCAM 2013: 571420.
Shafiei Neek, Gaeini AA, Choobineh S (2011) Effect of zinc and selenium supplementation on serum testosterone and plasma lactate in cyclist after an exhaustive exercise bout. Biol Trace Elem Res 144: 454-462.
Cinar V, Polat Y, Baltaci AK, Mogulkoc R (2011) Effects of magnesium supplementation on testosterone levels of athletes and sedentary subjects at rest and after exhaustion. Biol Trace Elem Res 140: 18-23.
Jana K, Samanta PK, Manna I, Ghosh P, Singh N, Khetan RP, Ray BR (2008) Protective effect of sodium selenite and zinc sulfate on intensive swimming-induced testicular gamatogenic and steroidogenic disorders in mature male rats. Appl Physiol Nutr Metab 33: 903-914.
Karp SM, Koch TR (2006) Oxidative stress and antioxidants in inflammatory bowel disease. Dis Mon 52: 199-207.