Biochemistry and Molecular Biology

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Insights on Heterologous Expression of Fungal Cellulases in Pichia pastoris

Received: 17 February 2018    Accepted: 27 March 2018    Published: 02 April 2018
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Abstract

Cellulosic biomass, the most remarkable renewable source of fuels and other compounds of potential importance, needs highly complex mixture of various enzymes for its degradation. For sustainable and economical bioprocess, the availability of these enzymes in high quantities and at a low price is much warranted. The advancements in discovery of new strains, highly valuable techniques and molecular biotechnological tools have led to Pichia pastoris gaining a recognized organism status both at industrial as well as laboratory level. With numerous beneficial characteristics on its credit P. pastoris has emerged a promising host for most of heterologous proteins production. Thus, the production of fungal cellulytic enzymes is of worth to be comprehensively illustrated. There are some activators including promoters used in the expression host to increase the utility of expression system. This review summarizes the heterologous expression of fungal cellulases (Exo-glucanases, endo-glucanases (EGs) and beta-glucosidases (BGLs)) and advocates the various kinds of promoters used in P. Pastoris for expression of these enzymes and proteins. Further, concluding remarks will provide insights revealing the scope of P. pastoris as a potential expression system tool in today's modern direction of research.

DOI 10.11648/j.bmb.20180301.13
Published in Biochemistry and Molecular Biology (Volume 3, Issue 1, January 2018)
Page(s) 15-35
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Pichia pastoris, Heterologous Expression, Fungal Cellulases, Promoters, Synthetic Core Promoters

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Author Information
  • School of Biotechnology, Jiangnan University, Wuxi, P. R. China

  • College of Plant Sciences and Technology, Huazhong Agricultural University, Wuhan, P. R. China

  • Interdisciplinary Research Centre in Biomedical Materials (IRCBM) COMSATS Institute of Information Technology, Lahore, Pakistan

  • School of Food Science and Technology, Jiangnan University, Wuxi, P. R. China

  • Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, P. R. China; Department of Biology, College of Science and Technology, University of Rwanda, Kigali, Rwanda

  • School of Biotechnology, Jiangnan University, Wuxi, P. R. China

  • School of Biotechnology, Jiangnan University, Wuxi, P. R. China

  • School of Biotechnology, Jiangnan University, Wuxi, P. R. China

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    Sajid Kamal, Shahid Ullah Khan, Nawshad Muhammad, Muhammad Shoaib, Mukama Omar, et al. (2018). Insights on Heterologous Expression of Fungal Cellulases in Pichia pastoris. Biochemistry and Molecular Biology, 3(1), 15-35. https://doi.org/10.11648/j.bmb.20180301.13

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    Sajid Kamal; Shahid Ullah Khan; Nawshad Muhammad; Muhammad Shoaib; Mukama Omar, et al. Insights on Heterologous Expression of Fungal Cellulases in Pichia pastoris. Biochem. Mol. Biol. 2018, 3(1), 15-35. doi: 10.11648/j.bmb.20180301.13

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    Sajid Kamal, Shahid Ullah Khan, Nawshad Muhammad, Muhammad Shoaib, Mukama Omar, et al. Insights on Heterologous Expression of Fungal Cellulases in Pichia pastoris. Biochem Mol Biol. 2018;3(1):15-35. doi: 10.11648/j.bmb.20180301.13

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  • @article{10.11648/j.bmb.20180301.13,
      author = {Sajid Kamal and Shahid Ullah Khan and Nawshad Muhammad and Muhammad Shoaib and Mukama Omar and Kaneza Pascal and Mukasekuru Marie Rose and Fubao Fuelbiol Sun},
      title = {Insights on Heterologous Expression of Fungal Cellulases in Pichia pastoris},
      journal = {Biochemistry and Molecular Biology},
      volume = {3},
      number = {1},
      pages = {15-35},
      doi = {10.11648/j.bmb.20180301.13},
      url = {https://doi.org/10.11648/j.bmb.20180301.13},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.bmb.20180301.13},
      abstract = {Cellulosic biomass, the most remarkable renewable source of fuels and other compounds of potential importance, needs highly complex mixture of various enzymes for its degradation. For sustainable and economical bioprocess, the availability of these enzymes in high quantities and at a low price is much warranted. The advancements in discovery of new strains, highly valuable techniques and molecular biotechnological tools have led to Pichia pastoris gaining a recognized organism status both at industrial as well as laboratory level. With numerous beneficial characteristics on its credit P. pastoris has emerged a promising host for most of heterologous proteins production. Thus, the production of fungal cellulytic enzymes is of worth to be comprehensively illustrated. There are some activators including promoters used in the expression host to increase the utility of expression system. This review summarizes the heterologous expression of fungal cellulases (Exo-glucanases, endo-glucanases (EGs) and beta-glucosidases (BGLs)) and advocates the various kinds of promoters used in P. Pastoris for expression of these enzymes and proteins. Further, concluding remarks will provide insights revealing the scope of P. pastoris as a potential expression system tool in today's modern direction of research.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Insights on Heterologous Expression of Fungal Cellulases in Pichia pastoris
    AU  - Sajid Kamal
    AU  - Shahid Ullah Khan
    AU  - Nawshad Muhammad
    AU  - Muhammad Shoaib
    AU  - Mukama Omar
    AU  - Kaneza Pascal
    AU  - Mukasekuru Marie Rose
    AU  - Fubao Fuelbiol Sun
    Y1  - 2018/04/02
    PY  - 2018
    N1  - https://doi.org/10.11648/j.bmb.20180301.13
    DO  - 10.11648/j.bmb.20180301.13
    T2  - Biochemistry and Molecular Biology
    JF  - Biochemistry and Molecular Biology
    JO  - Biochemistry and Molecular Biology
    SP  - 15
    EP  - 35
    PB  - Science Publishing Group
    SN  - 2575-5048
    UR  - https://doi.org/10.11648/j.bmb.20180301.13
    AB  - Cellulosic biomass, the most remarkable renewable source of fuels and other compounds of potential importance, needs highly complex mixture of various enzymes for its degradation. For sustainable and economical bioprocess, the availability of these enzymes in high quantities and at a low price is much warranted. The advancements in discovery of new strains, highly valuable techniques and molecular biotechnological tools have led to Pichia pastoris gaining a recognized organism status both at industrial as well as laboratory level. With numerous beneficial characteristics on its credit P. pastoris has emerged a promising host for most of heterologous proteins production. Thus, the production of fungal cellulytic enzymes is of worth to be comprehensively illustrated. There are some activators including promoters used in the expression host to increase the utility of expression system. This review summarizes the heterologous expression of fungal cellulases (Exo-glucanases, endo-glucanases (EGs) and beta-glucosidases (BGLs)) and advocates the various kinds of promoters used in P. Pastoris for expression of these enzymes and proteins. Further, concluding remarks will provide insights revealing the scope of P. pastoris as a potential expression system tool in today's modern direction of research.
    VL  - 3
    IS  - 1
    ER  - 

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