Biochemistry and Molecular Biology

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Antagonism of Dexamethasone to Radiation Bystander Effect and Its Re-transmission

Received: 08 October 2019    Accepted: 08 November 2019    Published: 15 November 2019
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Abstract

Background: In clinical radiotherapy, in addition to the symptoms of radiation damage in the irradiated area, normal tissues can produce damage other than direct irradiation due to bystander effects. We often use dexamethasone to relieve symptoms, thereby protecting the surrounding normal tissues, and may also protect the tissues outside the target area. Objectives: To observe the antagonism of dexamethasone on radiation bystander effect and its Re-transmission. Materials and methods: After 6MV X-ray irradiation of rabbit, the plasma, the first generation conditioned medium, was prepared to treat rabbit lymphocyte, cultured for 24 hours, 24 hours later, lymphocytes were collected, and the second generation conditioned medium was prepared with the lymphocyte secretion to treat new rabbit lymphocyte, and the apoptotic rate of effecter lymphocyte was detected. Dexamethasone was used as a parallel treatment control in all experimental groups. Results: The first and second generations of conditioned medium can stimulate lymphocyte apoptosis. Dexamethasone could reduce the apoptotic level of rabbit lymphocytes treated with the first and second generation bystander conditioned medium from 21.087% to 14.957%, 28.695% to 20.205%, respectively. Conclusion: Under certain conditions, dexamethasone can antagonize the injury of lymphocyte by radiation bystander effect, and can also antagonize the re-transmission of radiation bystander effect.

DOI 10.11648/j.bmb.20190405.12
Published in Biochemistry and Molecular Biology (Volume 4, Issue 5, September 2019)
Page(s) 74-79
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Radiation Bystander Effect, Re-transmission, Dexamethasone

References
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Author Information
  • School of Clinical, Graduate School of Xuzhou Medical University, Xuzhou, China

  • School of Clinical, Graduate School of Xuzhou Medical University, Xuzhou, China

  • School of Clinical, Graduate School of Xuzhou Medical University, Xuzhou, China; Department of Radiotherapy, Xuzhou Cancer Hospital, Xuzhou, China

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  • APA Style

    Zhan Hao, Yao Ruoyu, Zhang Xuguang. (2019). Antagonism of Dexamethasone to Radiation Bystander Effect and Its Re-transmission. Biochemistry and Molecular Biology, 4(5), 74-79. https://doi.org/10.11648/j.bmb.20190405.12

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    ACS Style

    Zhan Hao; Yao Ruoyu; Zhang Xuguang. Antagonism of Dexamethasone to Radiation Bystander Effect and Its Re-transmission. Biochem. Mol. Biol. 2019, 4(5), 74-79. doi: 10.11648/j.bmb.20190405.12

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    AMA Style

    Zhan Hao, Yao Ruoyu, Zhang Xuguang. Antagonism of Dexamethasone to Radiation Bystander Effect and Its Re-transmission. Biochem Mol Biol. 2019;4(5):74-79. doi: 10.11648/j.bmb.20190405.12

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  • @article{10.11648/j.bmb.20190405.12,
      author = {Zhan Hao and Yao Ruoyu and Zhang Xuguang},
      title = {Antagonism of Dexamethasone to Radiation Bystander Effect and Its Re-transmission},
      journal = {Biochemistry and Molecular Biology},
      volume = {4},
      number = {5},
      pages = {74-79},
      doi = {10.11648/j.bmb.20190405.12},
      url = {https://doi.org/10.11648/j.bmb.20190405.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.bmb.20190405.12},
      abstract = {Background: In clinical radiotherapy, in addition to the symptoms of radiation damage in the irradiated area, normal tissues can produce damage other than direct irradiation due to bystander effects. We often use dexamethasone to relieve symptoms, thereby protecting the surrounding normal tissues, and may also protect the tissues outside the target area. Objectives: To observe the antagonism of dexamethasone on radiation bystander effect and its Re-transmission. Materials and methods: After 6MV X-ray irradiation of rabbit, the plasma, the first generation conditioned medium, was prepared to treat rabbit lymphocyte, cultured for 24 hours, 24 hours later, lymphocytes were collected, and the second generation conditioned medium was prepared with the lymphocyte secretion to treat new rabbit lymphocyte, and the apoptotic rate of effecter lymphocyte was detected. Dexamethasone was used as a parallel treatment control in all experimental groups. Results: The first and second generations of conditioned medium can stimulate lymphocyte apoptosis. Dexamethasone could reduce the apoptotic level of rabbit lymphocytes treated with the first and second generation bystander conditioned medium from 21.087% to 14.957%, 28.695% to 20.205%, respectively. Conclusion: Under certain conditions, dexamethasone can antagonize the injury of lymphocyte by radiation bystander effect, and can also antagonize the re-transmission of radiation bystander effect.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Antagonism of Dexamethasone to Radiation Bystander Effect and Its Re-transmission
    AU  - Zhan Hao
    AU  - Yao Ruoyu
    AU  - Zhang Xuguang
    Y1  - 2019/11/15
    PY  - 2019
    N1  - https://doi.org/10.11648/j.bmb.20190405.12
    DO  - 10.11648/j.bmb.20190405.12
    T2  - Biochemistry and Molecular Biology
    JF  - Biochemistry and Molecular Biology
    JO  - Biochemistry and Molecular Biology
    SP  - 74
    EP  - 79
    PB  - Science Publishing Group
    SN  - 2575-5048
    UR  - https://doi.org/10.11648/j.bmb.20190405.12
    AB  - Background: In clinical radiotherapy, in addition to the symptoms of radiation damage in the irradiated area, normal tissues can produce damage other than direct irradiation due to bystander effects. We often use dexamethasone to relieve symptoms, thereby protecting the surrounding normal tissues, and may also protect the tissues outside the target area. Objectives: To observe the antagonism of dexamethasone on radiation bystander effect and its Re-transmission. Materials and methods: After 6MV X-ray irradiation of rabbit, the plasma, the first generation conditioned medium, was prepared to treat rabbit lymphocyte, cultured for 24 hours, 24 hours later, lymphocytes were collected, and the second generation conditioned medium was prepared with the lymphocyte secretion to treat new rabbit lymphocyte, and the apoptotic rate of effecter lymphocyte was detected. Dexamethasone was used as a parallel treatment control in all experimental groups. Results: The first and second generations of conditioned medium can stimulate lymphocyte apoptosis. Dexamethasone could reduce the apoptotic level of rabbit lymphocytes treated with the first and second generation bystander conditioned medium from 21.087% to 14.957%, 28.695% to 20.205%, respectively. Conclusion: Under certain conditions, dexamethasone can antagonize the injury of lymphocyte by radiation bystander effect, and can also antagonize the re-transmission of radiation bystander effect.
    VL  - 4
    IS  - 5
    ER  - 

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