Biochemistry and Molecular Biology

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Hepatoprotective Potentiality of Various Fractions of Ethanolic Extracts of Lawsonia Inermis (Henna) Leaves Against Chemical-Induced Hepatitis in Rats

Received: 25 July 2016    Accepted: 02 August 2016    Published: 21 August 2016
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Abstract

Liver is a metabolically active organ responsible for many vital life functions. More than 900 drugs, toxins, and herbs have been reported to cause liver injury. In spite of tremendous advances in modern medicine, there are hardly any reliable drugs that protect the liver from damage and/or help in regeneration of hepatic cell. It is, therefore, necessary to search for effective and safe herbal drugs for the treatment of liver disease. In the present study, we investigated the hepatoprotective activity of ethanolic extracts of various fractions (Ethyl acetate, Petroleum ether and Chloroform) of Lowsonia inermis leaves in carbon tetrachloride (CCl4)-induced hepatitis rats. Hepatotoxicity was induced in rats by intraperitoneal injection of CCl4 on alternate days for 7 days. After 7 days of pretreatment of test extracts, the biochemical markers such as Serum bilirubin, Serum Glutamic Pyruvic Transaminase (SGPT), Serum Glutamic Oxaloacetic Transaminase (SGOT) and Alkaline Phosphatase (ALP), were examined. The extracts at the doses of 200 mg/kg p.o. significantly (P < 0.05) reduced the elevated levels of Serum bilirubin, SGPT, SGOT and SALP compared to the CCl4-treated group alone. The findings of the study indicate that the leaf extract of L. inermis showed a potential hepatoprotective activity and the protective action might be manifested by reducing the oxidative stress as the leaf extracts possess flavonoid and phenolic compounds which have free radical scavenging and antioxidant activity. However, further study is necessary uncovering the exact molecular mechanism of hepatoprotective activity.

DOI 10.11648/j.bmb.20160102.12
Published in Biochemistry and Molecular Biology (Volume 1, Issue 2, September 2016)
Page(s) 17-22
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Antioxidant Activity, Carbon Tetrachloride, Ethanolic Extracts, Hepatoprotective Activity

References
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[2] Recknagel, R. O. (1983). "A new direction in the study of carbon tetrachloride hepatotoxicity." Life Sciences, 33: 401-408.
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[6] Khan, M. R. I., et al. (2010). "Antidiabetic Effects of the Different Fractions of Ethanolic Extracts of Ocimum sanctum in Normal and Alloxan Induced Diabetic Rats." J Sci Res., 2(1): 158-168.
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[11] Prieto, P., et al. (1999). "Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E." Analytical biochemistry, 269(2): 337-341.
[12] Communities., C. o. E. (1986). "Council instructions about the protection of living animals used in scientific investigations." Official Journal of the European Communities, (JO 86/609/CEE), L358; Brussels: 1-18.
[13] Mahmud, Z. A., et al. (2012). "Antioxidant and Hepatoprotective Activities of Ethanolic Extracts of Leaves of Premna esculenta Roxb. against Carbon Tetrachloride-Induced Liver Damage in Rats." Journal of Young Pharmacists, 4(4): 228-234.
[14] Nishigaki, I., et al. (1992). "Suppressive effect of curcumin on lipid peroxidation induced in rats by carbon tetrachloride or Co 60 irradiation." J Clin Biochem Nutr., 13: 23-29.
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[16] Bergmeyer, H. U. (1980). "IFCC methods for the measurement of catalytic concentrations of enzymes: Part 3. IFCC method for alanine aminotransferase (L-alanine: 2-oxoglutarate aminotransferase, EC 2.6.1.2)." Clin Chim Acta, 105: 1147-1154.
[17] Henley, K. S. (1980). "IFCC method for alanine aminotransferase Stage 2, Draft 1, 1979-11-09." Clin Chim Acta, 105: 155-166.
[18] McComb, R. B. and Bowers G. N. J. (1972). "Study of optimum buffer conditions for measuring alkaline phosphatase activity in human serum." Clin Chem Acta, 18: 97-104.
[19] Sule, O. J., et al. (2012). "Biochemical Investigation of Hepatotoxic effects of Antiretroviral Drugs on Wistar Albino Rats."Journal of Physiology and Pharmacology Advances, 2(4): 171-175.
[20] Gaskill, C. L., et al. (2005). "Liver histopathology and liver and serum alanine aminotransferase and alkaline phosphatase activities in epileptic dogs receiving phenobarbital." Veterinary Pathology, 42(2): 147-160.
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Author Information
  • Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh

  • Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh

  • Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh

  • Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh

  • Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh

  • Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh;Department of Organ Anatomy, Tohoku University, Tohoku, Japan

  • Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh

  • Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh

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    Khondakar Mahmudul Hasan, Samina Yesmin, Sarkar Farhana Akhter, Subrata Paul, Sabarni Sarker, et al. (2016). Hepatoprotective Potentiality of Various Fractions of Ethanolic Extracts of Lawsonia Inermis (Henna) Leaves Against Chemical-Induced Hepatitis in Rats. Biochemistry and Molecular Biology, 1(2), 17-22. https://doi.org/10.11648/j.bmb.20160102.12

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    ACS Style

    Khondakar Mahmudul Hasan; Samina Yesmin; Sarkar Farhana Akhter; Subrata Paul; Sabarni Sarker, et al. Hepatoprotective Potentiality of Various Fractions of Ethanolic Extracts of Lawsonia Inermis (Henna) Leaves Against Chemical-Induced Hepatitis in Rats. Biochem. Mol. Biol. 2016, 1(2), 17-22. doi: 10.11648/j.bmb.20160102.12

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    AMA Style

    Khondakar Mahmudul Hasan, Samina Yesmin, Sarkar Farhana Akhter, Subrata Paul, Sabarni Sarker, et al. Hepatoprotective Potentiality of Various Fractions of Ethanolic Extracts of Lawsonia Inermis (Henna) Leaves Against Chemical-Induced Hepatitis in Rats. Biochem Mol Biol. 2016;1(2):17-22. doi: 10.11648/j.bmb.20160102.12

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  • @article{10.11648/j.bmb.20160102.12,
      author = {Khondakar Mahmudul Hasan and Samina Yesmin and Sarkar Farhana Akhter and Subrata Paul and Sabarni Sarker and Md Ariful Islam and Mir Imam Ibne Wahed and Md Rafiqul Islam Khan},
      title = {Hepatoprotective Potentiality of Various Fractions of Ethanolic Extracts of Lawsonia Inermis (Henna) Leaves Against Chemical-Induced Hepatitis in Rats},
      journal = {Biochemistry and Molecular Biology},
      volume = {1},
      number = {2},
      pages = {17-22},
      doi = {10.11648/j.bmb.20160102.12},
      url = {https://doi.org/10.11648/j.bmb.20160102.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.bmb.20160102.12},
      abstract = {Liver is a metabolically active organ responsible for many vital life functions. More than 900 drugs, toxins, and herbs have been reported to cause liver injury. In spite of tremendous advances in modern medicine, there are hardly any reliable drugs that protect the liver from damage and/or help in regeneration of hepatic cell. It is, therefore, necessary to search for effective and safe herbal drugs for the treatment of liver disease. In the present study, we investigated the hepatoprotective activity of ethanolic extracts of various fractions (Ethyl acetate, Petroleum ether and Chloroform) of Lowsonia inermis leaves in carbon tetrachloride (CCl4)-induced hepatitis rats. Hepatotoxicity was induced in rats by intraperitoneal injection of CCl4 on alternate days for 7 days. After 7 days of pretreatment of test extracts, the biochemical markers such as Serum bilirubin, Serum Glutamic Pyruvic Transaminase (SGPT), Serum Glutamic Oxaloacetic Transaminase (SGOT) and Alkaline Phosphatase (ALP), were examined. The extracts at the doses of 200 mg/kg p.o. significantly (P 4-treated group alone. The findings of the study indicate that the leaf extract of L. inermis showed a potential hepatoprotective activity and the protective action might be manifested by reducing the oxidative stress as the leaf extracts possess flavonoid and phenolic compounds which have free radical scavenging and antioxidant activity. However, further study is necessary uncovering the exact molecular mechanism of hepatoprotective activity.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Hepatoprotective Potentiality of Various Fractions of Ethanolic Extracts of Lawsonia Inermis (Henna) Leaves Against Chemical-Induced Hepatitis in Rats
    AU  - Khondakar Mahmudul Hasan
    AU  - Samina Yesmin
    AU  - Sarkar Farhana Akhter
    AU  - Subrata Paul
    AU  - Sabarni Sarker
    AU  - Md Ariful Islam
    AU  - Mir Imam Ibne Wahed
    AU  - Md Rafiqul Islam Khan
    Y1  - 2016/08/21
    PY  - 2016
    N1  - https://doi.org/10.11648/j.bmb.20160102.12
    DO  - 10.11648/j.bmb.20160102.12
    T2  - Biochemistry and Molecular Biology
    JF  - Biochemistry and Molecular Biology
    JO  - Biochemistry and Molecular Biology
    SP  - 17
    EP  - 22
    PB  - Science Publishing Group
    SN  - 2575-5048
    UR  - https://doi.org/10.11648/j.bmb.20160102.12
    AB  - Liver is a metabolically active organ responsible for many vital life functions. More than 900 drugs, toxins, and herbs have been reported to cause liver injury. In spite of tremendous advances in modern medicine, there are hardly any reliable drugs that protect the liver from damage and/or help in regeneration of hepatic cell. It is, therefore, necessary to search for effective and safe herbal drugs for the treatment of liver disease. In the present study, we investigated the hepatoprotective activity of ethanolic extracts of various fractions (Ethyl acetate, Petroleum ether and Chloroform) of Lowsonia inermis leaves in carbon tetrachloride (CCl4)-induced hepatitis rats. Hepatotoxicity was induced in rats by intraperitoneal injection of CCl4 on alternate days for 7 days. After 7 days of pretreatment of test extracts, the biochemical markers such as Serum bilirubin, Serum Glutamic Pyruvic Transaminase (SGPT), Serum Glutamic Oxaloacetic Transaminase (SGOT) and Alkaline Phosphatase (ALP), were examined. The extracts at the doses of 200 mg/kg p.o. significantly (P 4-treated group alone. The findings of the study indicate that the leaf extract of L. inermis showed a potential hepatoprotective activity and the protective action might be manifested by reducing the oxidative stress as the leaf extracts possess flavonoid and phenolic compounds which have free radical scavenging and antioxidant activity. However, further study is necessary uncovering the exact molecular mechanism of hepatoprotective activity.
    VL  - 1
    IS  - 2
    ER  - 

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