Constitutive Expression of <i>VvAATP</i> Increases Starch Content in Transgenic <i>Arabidopsis</i>

Feibing Wang; Bin Weng; Qi Wang; Yuxiu Ye; Jing Zhang; Gaolei Ren; Bowen Wang; Qing Zhou; Xinhong Chen

doi:doi:10.11648/j.bmb.20170206.15

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Constitutive Expression of VvAATP Increases Starch Content in Transgenic Arabidopsis

Feibing Wang, Bin Weng, Qi Wang, Yuxiu Ye, Jing Zhang, Gaolei Ren, Bowen Wang, Qing Zhou, Xinhong Chen

Published in Biochemistry and Molecular Biology (Volume 2, Issue 6)

Received: 5 September 2017 Accepted: 18 October 2017 Published: 22 November 2017

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Abstract

A plastidic ATP/ADP transporter (AATP) plays a crucial role in importing ATP from the cytosol into plastids, leading to the increase the ATP supply to facilitate anabolic synthesis in heterotrophic plastids of dicotyledonous plants. The regulatory role of the grapevine VvAATP gene in increasing starch accumulation has not been investigated. In this study, the VvAATP gene was successfully isolated from grapevine and transformed into Arabidopsis. Constitutive expression of VvAATP significantly increased starch accumulation in transgenic Arabidopsis plants. Real-time quantitative PCR analysis showed that constitutive expression of VvAATP up-regulated the expression of the genes related to starch biosynthesis pathway, including phosphoglucomutase, ADP-glucose pyrophosphorylase (AGPase), granule-bound starch synthase (GBSS), soluble starch synthase (SSS) and starch branching enzyme (SBE) genes, in transgenic Arabidopsis plants. Meanwhile, enzymatic analyses indicated that the major enzymes (AGPase, GBSS, SSS and SBE) involved in the starch biosynthesis exhibited higher activities in the transgenic plants compared to the wild-type (WT). These results indicate that VvAATP may improve starch content of Arabidopsis by up-regulating the expression of the related genes and increasing the activities of the major enzymes invovled in starch biosynthesis. All these findings suggest that the VvAATP gene may be applied for increasing starch accumulation of plants in the future.

Published in	Biochemistry and Molecular Biology (Volume 2, Issue 6)
DOI	10.11648/j.bmb.20170206.15
Page(s)	94-101
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Arabidopsis, Constitutive Expression, Grapevine, Starch Content, VvAATP

References

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APA Style

Feibing Wang, Bin Weng, Qi Wang, Yuxiu Ye, Jing Zhang, et al. (2017). Constitutive Expression of VvAATP Increases Starch Content in Transgenic Arabidopsis. Biochemistry and Molecular Biology, 2(6), 94-101. https://doi.org/10.11648/j.bmb.20170206.15

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ACS Style

Feibing Wang; Bin Weng; Qi Wang; Yuxiu Ye; Jing Zhang, et al. Constitutive Expression of VvAATP Increases Starch Content in Transgenic Arabidopsis. Biochem. Mol. Biol. 2017, 2(6), 94-101. doi: 10.11648/j.bmb.20170206.15

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AMA Style

Feibing Wang, Bin Weng, Qi Wang, Yuxiu Ye, Jing Zhang, et al. Constitutive Expression of VvAATP Increases Starch Content in Transgenic Arabidopsis. Biochem Mol Biol. 2017;2(6):94-101. doi: 10.11648/j.bmb.20170206.15

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@article{10.11648/j.bmb.20170206.15,
  author = {Feibing Wang and Bin Weng and Qi Wang and Yuxiu Ye and Jing Zhang and Gaolei Ren and Bowen Wang and Qing Zhou and Xinhong Chen},
  title = {Constitutive Expression of VvAATP Increases Starch Content in Transgenic Arabidopsis},
  journal = {Biochemistry and Molecular Biology},
  volume = {2},
  number = {6},
  pages = {94-101},
  doi = {10.11648/j.bmb.20170206.15},
  url = {https://doi.org/10.11648/j.bmb.20170206.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20170206.15},
  abstract = {A plastidic ATP/ADP transporter (AATP) plays a crucial role in importing ATP from the cytosol into plastids, leading to the increase the ATP supply to facilitate anabolic synthesis in heterotrophic plastids of dicotyledonous plants. The regulatory role of the grapevine VvAATP gene in increasing starch accumulation has not been investigated. In this study, the VvAATP gene was successfully isolated from grapevine and transformed into Arabidopsis. Constitutive expression of VvAATP significantly increased starch accumulation in transgenic Arabidopsis plants. Real-time quantitative PCR analysis showed that constitutive expression of VvAATP up-regulated the expression of the genes related to starch biosynthesis pathway, including phosphoglucomutase, ADP-glucose pyrophosphorylase (AGPase), granule-bound starch synthase (GBSS), soluble starch synthase (SSS) and starch branching enzyme (SBE) genes, in transgenic Arabidopsis plants. Meanwhile, enzymatic analyses indicated that the major enzymes (AGPase, GBSS, SSS and SBE) involved in the starch biosynthesis exhibited higher activities in the transgenic plants compared to the wild-type (WT). These results indicate that VvAATP may improve starch content of Arabidopsis by up-regulating the expression of the related genes and increasing the activities of the major enzymes invovled in starch biosynthesis. All these findings suggest that the VvAATP gene may be applied for increasing starch accumulation of plants in the future.},
 year = {2017}
}

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TY  - JOUR
T1  - Constitutive Expression of VvAATP Increases Starch Content in Transgenic Arabidopsis
AU  - Feibing Wang
AU  - Bin Weng
AU  - Qi Wang
AU  - Yuxiu Ye
AU  - Jing Zhang
AU  - Gaolei Ren
AU  - Bowen Wang
AU  - Qing Zhou
AU  - Xinhong Chen
Y1  - 2017/11/22
PY  - 2017
N1  - https://doi.org/10.11648/j.bmb.20170206.15
DO  - 10.11648/j.bmb.20170206.15
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 94
EP  - 101
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20170206.15
AB  - A plastidic ATP/ADP transporter (AATP) plays a crucial role in importing ATP from the cytosol into plastids, leading to the increase the ATP supply to facilitate anabolic synthesis in heterotrophic plastids of dicotyledonous plants. The regulatory role of the grapevine VvAATP gene in increasing starch accumulation has not been investigated. In this study, the VvAATP gene was successfully isolated from grapevine and transformed into Arabidopsis. Constitutive expression of VvAATP significantly increased starch accumulation in transgenic Arabidopsis plants. Real-time quantitative PCR analysis showed that constitutive expression of VvAATP up-regulated the expression of the genes related to starch biosynthesis pathway, including phosphoglucomutase, ADP-glucose pyrophosphorylase (AGPase), granule-bound starch synthase (GBSS), soluble starch synthase (SSS) and starch branching enzyme (SBE) genes, in transgenic Arabidopsis plants. Meanwhile, enzymatic analyses indicated that the major enzymes (AGPase, GBSS, SSS and SBE) involved in the starch biosynthesis exhibited higher activities in the transgenic plants compared to the wild-type (WT). These results indicate that VvAATP may improve starch content of Arabidopsis by up-regulating the expression of the related genes and increasing the activities of the major enzymes invovled in starch biosynthesis. All these findings suggest that the VvAATP gene may be applied for increasing starch accumulation of plants in the future.
VL  - 2
IS  - 6
ER  -

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Author Information

Feibing Wang

School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, China
Bin Weng

School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, China
Qi Wang

School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, China
Yuxiu Ye

School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, China
Jing Zhang

School of Chemical Engineering, Huaiyin Institute of Technology, Huai’an, China
Gaolei Ren

School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, China
Bowen Wang

School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, China
Qing Zhou

School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, China
Xinhong Chen

School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, China

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Plain Text BibTeX RIS

APA Style

Feibing Wang, Bin Weng, Qi Wang, Yuxiu Ye, Jing Zhang, et al. (2017). Constitutive Expression of VvAATP Increases Starch Content in Transgenic Arabidopsis. Biochemistry and Molecular Biology, 2(6), 94-101. https://doi.org/10.11648/j.bmb.20170206.15

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ACS Style

Feibing Wang; Bin Weng; Qi Wang; Yuxiu Ye; Jing Zhang, et al. Constitutive Expression of VvAATP Increases Starch Content in Transgenic Arabidopsis. Biochem. Mol. Biol. 2017, 2(6), 94-101. doi: 10.11648/j.bmb.20170206.15

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AMA Style

Feibing Wang, Bin Weng, Qi Wang, Yuxiu Ye, Jing Zhang, et al. Constitutive Expression of VvAATP Increases Starch Content in Transgenic Arabidopsis. Biochem Mol Biol. 2017;2(6):94-101. doi: 10.11648/j.bmb.20170206.15

Copy | Download

@article{10.11648/j.bmb.20170206.15,
  author = {Feibing Wang and Bin Weng and Qi Wang and Yuxiu Ye and Jing Zhang and Gaolei Ren and Bowen Wang and Qing Zhou and Xinhong Chen},
  title = {Constitutive Expression of VvAATP Increases Starch Content in Transgenic Arabidopsis},
  journal = {Biochemistry and Molecular Biology},
  volume = {2},
  number = {6},
  pages = {94-101},
  doi = {10.11648/j.bmb.20170206.15},
  url = {https://doi.org/10.11648/j.bmb.20170206.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20170206.15},
  abstract = {A plastidic ATP/ADP transporter (AATP) plays a crucial role in importing ATP from the cytosol into plastids, leading to the increase the ATP supply to facilitate anabolic synthesis in heterotrophic plastids of dicotyledonous plants. The regulatory role of the grapevine VvAATP gene in increasing starch accumulation has not been investigated. In this study, the VvAATP gene was successfully isolated from grapevine and transformed into Arabidopsis. Constitutive expression of VvAATP significantly increased starch accumulation in transgenic Arabidopsis plants. Real-time quantitative PCR analysis showed that constitutive expression of VvAATP up-regulated the expression of the genes related to starch biosynthesis pathway, including phosphoglucomutase, ADP-glucose pyrophosphorylase (AGPase), granule-bound starch synthase (GBSS), soluble starch synthase (SSS) and starch branching enzyme (SBE) genes, in transgenic Arabidopsis plants. Meanwhile, enzymatic analyses indicated that the major enzymes (AGPase, GBSS, SSS and SBE) involved in the starch biosynthesis exhibited higher activities in the transgenic plants compared to the wild-type (WT). These results indicate that VvAATP may improve starch content of Arabidopsis by up-regulating the expression of the related genes and increasing the activities of the major enzymes invovled in starch biosynthesis. All these findings suggest that the VvAATP gene may be applied for increasing starch accumulation of plants in the future.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Constitutive Expression of VvAATP Increases Starch Content in Transgenic Arabidopsis
AU  - Feibing Wang
AU  - Bin Weng
AU  - Qi Wang
AU  - Yuxiu Ye
AU  - Jing Zhang
AU  - Gaolei Ren
AU  - Bowen Wang
AU  - Qing Zhou
AU  - Xinhong Chen
Y1  - 2017/11/22
PY  - 2017
N1  - https://doi.org/10.11648/j.bmb.20170206.15
DO  - 10.11648/j.bmb.20170206.15
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 94
EP  - 101
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20170206.15
AB  - A plastidic ATP/ADP transporter (AATP) plays a crucial role in importing ATP from the cytosol into plastids, leading to the increase the ATP supply to facilitate anabolic synthesis in heterotrophic plastids of dicotyledonous plants. The regulatory role of the grapevine VvAATP gene in increasing starch accumulation has not been investigated. In this study, the VvAATP gene was successfully isolated from grapevine and transformed into Arabidopsis. Constitutive expression of VvAATP significantly increased starch accumulation in transgenic Arabidopsis plants. Real-time quantitative PCR analysis showed that constitutive expression of VvAATP up-regulated the expression of the genes related to starch biosynthesis pathway, including phosphoglucomutase, ADP-glucose pyrophosphorylase (AGPase), granule-bound starch synthase (GBSS), soluble starch synthase (SSS) and starch branching enzyme (SBE) genes, in transgenic Arabidopsis plants. Meanwhile, enzymatic analyses indicated that the major enzymes (AGPase, GBSS, SSS and SBE) involved in the starch biosynthesis exhibited higher activities in the transgenic plants compared to the wild-type (WT). These results indicate that VvAATP may improve starch content of Arabidopsis by up-regulating the expression of the related genes and increasing the activities of the major enzymes invovled in starch biosynthesis. All these findings suggest that the VvAATP gene may be applied for increasing starch accumulation of plants in the future.
VL  - 2
IS  - 6
ER  -

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