The Study of the Radiation Protection of Aged Garlic Extract to the Radiation Effects in Male Rat’s Sperm
International Journal of Biochemistry, Biophysics & Molecular Biology
Volume 1, Issue 2, December 2016, Pages: 36-41
Received: Oct. 22, 2016; Accepted: Nov. 23, 2016; Published: Jan. 5, 2017
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Kouam Foubi Brice Bertrand, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon
Chuisseu Djamen Dieudonné Pascal, Faculty of Health Sciences, Université des Montagnes, Bangangté, Bangangté, Cameroon
Dzeufiet Djomeni Paul Désiré, Faculty of Sciences, University of Yaoundé I, Yaoundé, Cameroon
Samba Ngano Odette, Faculty of Sciences, University of Dschang, Dschang, Cameroon
Moifo Boniface, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon
Zeh Odile Fernande, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon
Guegang Goujou Emilienne, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon
Mbede Maggy, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon
Tiedeu Alain Bertin, National Advanced School of Engineering, University of Yaoundé I, Yaoundé, Cameroon
Gonsu Fotsin Joseph, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon
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Irradiation results in a depression of the sperm count. The risk of damage to genes and chromosomal of spermatozoa exists and increases with increasing dose, potentially causing sterility or developments defects in children. Reputed to be a powerful natural antioxidant that may cause inhibition of radical processes, the protection of the macromolecules essential for cell survival, and limiting oxygen effect, Aged garlic extract (AGE) has been demonstrated to possess several physiological activities in experimental animals thus, the present study aimed to asses if AGE was able to modulate the effects of gamma radiation-induced injury to sperm count. 80 healthy male rats were randomly allotted according to the duration of the experiment into 2 lots (Lot 1: preventive aspect and Lot 2: Curative aspect) of 40 rats each. The animals of each Lot were randomly divided into four equally and double male rat groups, five rats each, among which, 20 irradiated and 20 Sham Irradiated. Sperm motility was appreciated the 8th day post irradiation by counting under a microscope the spermatozoa contained in a determined volume of homogenate of the tail of the epididymis and the sperm density determined using the cell MALASSEZ. Exposure of rats to gamma irradiation caused a significant disturbance in sperm count but in rats exposed to radiation then pre-treated and treated with AGE, the results showed an improvement. Our results prove that AGE has protective effects against radiation-induced changes in sperm count post irradiation. Thus, it could be concluded that AGE might reduce the biological hazards induced by gamma irradiation in rat’s sperm.
Irradiation, Sperm, AGE, Epididymis, Rats
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Kouam Foubi Brice Bertrand, Chuisseu Djamen Dieudonné Pascal, Dzeufiet Djomeni Paul Désiré, Samba Ngano Odette, Moifo Boniface, Zeh Odile Fernande, Guegang Goujou Emilienne, Mbede Maggy, Tiedeu Alain Bertin, Gonsu Fotsin Joseph, The Study of the Radiation Protection of Aged Garlic Extract to the Radiation Effects in Male Rat’s Sperm, International Journal of Biochemistry, Biophysics & Molecular Biology. Vol. 1, No. 2, 2016, pp. 36-41. doi: 10.11648/j.ijbbmb.20160102.11
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