Compare the Chlorophyll Amount in Three Brown Algae Species of the Persian Gulf by Using Three Solvents and Applying Two Formulas
International Journal of Biochemistry, Biophysics & Molecular Biology
Volume 2, Issue 6, December 2017, Pages: 77-79
Received: Oct. 14, 2017;
Accepted: Oct. 26, 2017;
Published: Nov. 22, 2017
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Yasaman Etemadian, Faculty of Fisheries Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
Bahareh Shabanpour, Faculty of Fisheries Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
Vida Ghaemi, Department of Fisheries, Faculty of Natural Resources, Zabol University, Zabol, Iran
Moazameh Kordjazi, Faculty of Fisheries Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
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Chlorophyll is the green pigment in plants that helps to absorb sunlight and converts it into energy. It is believed that it is beneficial for the human body. Therefore in this study, the chlorophyll amount in three brown algae species (Sirophysalis trinodis, Polycladia myrica and Colpomenia sinuosa) of the Persian Gulf by using three solvents and applying two formulas was compared. Acetone, diethyl ether and methanol were used as solvents. Results showed that the chlorophyll amount in different species dependent on solvent type and cell wall structure of algae. The highest chlorophyll a and x+c contents were observed in S. trinodis species. Also, the highest content of chlorophyll c1+c2 was showed in C. sinuosa by the extraction with diethyl ether solvent. In both formulas was no significant difference between the same solvents in the amount of chlorophyll a. In general, the aim of this study due to the diversity and dispersion of algae and also their potential use in the food industry, cosmetics and pharmaceutical goods was to choose a suitable solvent for better extraction of chlorophyll as an active ingredient. Hence, in this study, the acetone was a better solvent.
Chlorophyll, Brown Algae, Solvent, Formula, Persian Gulf
To cite this article
Compare the Chlorophyll Amount in Three Brown Algae Species of the Persian Gulf by Using Three Solvents and Applying Two Formulas, International Journal of Biochemistry, Biophysics & Molecular Biology.
Vol. 2, No. 6,
2017, pp. 77-79.
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/
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Christaki E, Bonos E, Giannenas I, Florou-Paneri P (2013) Functional properties of carotenoids originating from algae. J Sci Food Agri 93: 5–11.
Dere S, Gunes T, Sivaci R (1998) Spectrophotometric Determination of Chlorophyll - A, B and Total Carotenoid Contents of Some Algae Species Using Different Solvents. Turk J Botany 22: 13-17.
Hsu CY, Chao PY, Hu SP, Yang CM (2013) The antioxidant and free radical scavenging activities of chlorophylls and pheophytins. Food Nut Sci 4: 1-8.
Huang TL, Cong HB (2007) A New Method for Determination of Chlorophylls in Freshwater Algae. Environ Monit Assess 129: 1–7.
Inanc AL (2011) Chlorophyll: Structural Properties, Health Benefits and Its Occurrence in Virgin Olive Oils. Akademik Gıda 9: 26-32.
Jeffrey SW, Humphrey GF (1975) New spectrophotometric equations for determining chlorophylls a, b, C1 and C2 in higher plants, algae and natural phytoplankton. Biochem physiol Pflanzen 167: 191-194.
Lenore SC, Amold EG, Aadrew DE (2001) Standard Methods for the Examination of Water and Wastewater. American Public Health Association, American Water Works Association, Water Environment Federation. p. 235–255.
Lee RE (2008) Phycology. 4th ed. Cambridge, England, Cambridge University press.
Sakthivel R, Devi KP (2015) Evaluation of physicochemical properties, proximate and nutritional composition of Gracilaria edulis collected from Palk Bay. J Food Chem 174: 68–74.
Sartoryl DP, Grobbelaas JU (1984) Extraction of chlorophyll a from freshwater phytoplankton for spectrophotometric analysis. Hydrobiologia 114: 177-187.
Wellburn AR (1994) The Spectral Determination of Chlorophylls a and b, as well as Total Carotenoids, Using Various Solvents with Spectrophotometers of Different Resolution. J Plant Physiol 144: 307-31.