International Journal of Biochemistry, Biophysics & Molecular Biology
Volume 3, Issue 1, March 2018, Pages: 1-9
Received: Jan. 2, 2018;
Accepted: Jan. 12, 2018;
Published: Jan. 23, 2018
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Dianne Heather Vincent, Trivedi Global, Inc., Henderson, USA
Mahendra Kumar Trivedi, Trivedi Global, Inc., Henderson, USA
Alice Branton, Trivedi Global, Inc., Henderson, USA
Dahryn Trivedi, Trivedi Global, Inc., Henderson, USA
Gopal Nayak, Trivedi Global, Inc., Henderson, USA
Sambhu Charan Mondal, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India
Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India
The objective of this experiment was to find out the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health parameters. The test items (viz. vitamin D3 and DMEM), were divided into two parts. One part of each test item was received the Biofield Energy Treatment by Dianne Heather Vincent and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Alkaline phosphatase (ALP), collagen, and bone mineralization activities were performed to assess bone activity in human bone osteosarcoma cells (MG-63). The test samples were found as safe in the tested concentrations by MTT assay. ALP was significantly increased by 82.87%, 30.90%, and 58.53% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item, respectively at 50 µg/mL than the UT-DMEM + UT-Test item group. Other parameter like collagen was significantly increased by 11.41% and 34.65% in the in the BT-DMEM + UT-Test item group at 1 and 10 µg/mL, respectively as compared to the untreated group. Besides, the percent of bone mineralization was distinctly increased by 148.81% and 251.39% in the UT-DMEM + BT-Test item and BT-DMEM + BT-Test item groups, respectively at 10 µg/mL compared to the untreated group. Further, percent of bone mineralization was distinctly increased by 112.36%, 124.88%, and 124.69% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 50 µg/mL compared to the untreated group. Moreover, at 100 µg/mL the percent of bone mineralization was remarkably increased by 85.97%, 102.63%, and 45.61% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively with respect to the UT-DMEM + UT-Test item group. Overall, the Biofield Energy Treated vitamin D3 was significantly improved the bone health parameters and it could be a powerful alternative nutraceutical supplement to combat vitamin D3 deficiency and fight against various bone related problems including rickets, osteomalacia, osteoporosis, osteogenesis imperfecta, Paget’s disease, bone and joint pain, low bone density, bone fractures, osteoma, chondrodystrophia fetalis, stress management and prevention, autoimmune and inflammatory diseases, and anti-aging by improving overall health.
Dianne Heather Vincent,
Mahendra Kumar Trivedi,
Sambhu Charan Mondal,
Influenced of Biofield Energy Healing Treatment on Vitamin D3 for the Assessment of Bone Health Parameters in MG-63 cells, International Journal of Biochemistry, Biophysics & Molecular Biology.
Vol. 3, No. 1,
2018, pp. 1-9.
Holick MF (2004) Sunlight and vitamin D for bone health and prevention of autoimmune diseases cancers, and cardiovascular disease. Am J Clin Nut 80: 1678S-1688S.
Holick MF (1996) Vitamin D and bone health. J Nutr 126: 1159S-1164S.
Matsuoka LY, Ide L, Wortsman J, MacLaughlin JA, Holick MF (1987) Sunscreens suppress vitamin D3 synthesis. J Clin Endocrinol Metab 64: 1165-1168.
Barnes MS, Robson JP, Bonham MP, Strain J, Wallace J (2006) Vitamin D: Status, supplementation and immunomodulation. Cur Nut Food Sci 2: 315-336.
Laird E, Ward M, McSorley E, Strain JJ, Wallace J (2010) Vitamin D and bone health; Potential mechanisms. Nutrients 2: 693-724.
Bhattarai T, Bhattacharya K, Chaudhuri P, Sengupta P (2014) Correlation of common biochemical markers for bone turnover, serum calcium, and alkaline phosphatase in post-menopausal women. The Malaysian Journal of Medical Sciences : MJMS 21: 58-61.
Iba K, Takada J, Yamashita T (2004) The serum level of bone-specific alkaline phosphatase activity is associated with aortic calcification in osteoporosis patients. J Bone Miner Metab 22: 594-596.
Holick MF, Garabedian M (2006) Vitamin D: Photobiology, metabolism, mechanism of action, and clinical applications. Primer on the metabolic bone diseases and disorders of mineral metabolism. Edited by: Favus MJ, Washington, DC.
DeLuca HF (2004) Overview of general physiologic features and functions of vitamin D. Am J Clin Nutr 80: 1689S-1696S.
Viguet-Carrin S, Garnero P, Delmas PD (2006) The role of collagen in bone strength. Osteoporos Int 17: 319-336.
Sroga GE, Vashishth D (2012) Effects of bone matrix proteins on fracture and fragility in osteoporosis. Curr Osteoporos Rep 10: 141-150.
Lutgendorf SK, Mullen-Houser E, Russell D, Degeest K, Jacobson G, Hart L, Bender D, Anderson B, Buekers TE, Goodheart MJ, Antoni MH, Sood AK, Lubaroff DM (2010) Preservation of immune function in cervical cancer patients during chemoradiation using a novel integrative approach. Brain Behav and Immun 24: 1231-1240.
Ironson G, Field T, Scafidi F, Hashimoto M, Kumar M, Kumar A, Price A, Goncalves A, Burman I, Tetenman C, Patarca R, Fletcher MA (1996) Massage therapy is associated with enhancement of the immune system's cytotoxic capacity. Int J Neurosci 84: 205-217.
Jain S, Hammerschlag R, Mills P, Cohen L, Krieger R, Vieten C, Lutgendorf S (2015) Clinical studies of biofield therapies: Summary, methodological challenges, and recommendations. Glob Adv Health Med 4: 58-66.
Rubik B (2002) The biofield hypothesis: Its biophysical basis and role in medicine. J Altern Complement Med 8: 703-717.
Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The potential impact of biofield treatment on human brain tumor cells: A time-lapse video microscopy. J Integr Oncol 4: 141.
Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) In vitro evaluation of biofield treatment on cancer biomarkers involved in endometrial and prostate cancer cell lines. J Cancer Sci Ther 7: 253-257.
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Antibiogram, biochemical reactions and biotyping of biofield treated Providencia rettgeri. American Journal of Health Research 3: 344-351.
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Antimicrobial sensitivity, biochemical characteristics and biotyping of Staphylococcus saprophyticus: An impact of biofield energy treatment. J Women’s Health Care 4: 271.
Trivedi MK, Branton A, Trivedi D, Nayak G, Shettigar H, Mondal SC, Jana S (2015) Antimicrobial susceptibility pattern, biochemical characteristics and biotyping of Salmonella paratyphi A: An impact of biofield treatment. Clin Microbiol 4: 215.
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Antibiogram of biofield-treated Shigella boydii: Global burden of infections. Science Journal of Clinical Medicine 4: 121-126.
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of antibiogram, genotype and phylogenetic analysis of biofield treated Nocardia otitidis. Biol Syst Open Access 4: 143.
Trivedi MK, Branton A, Trivedi D, Nayak G, Charan S, Jana S (2015) Phenotyping and 16S rDNA analysis after biofield treatment on Citrobacter braakii: A urinary pathogen. J Clin Med Genom 3: 129.
Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Spectroscopic characterization of chloramphenicol and tetracycline: An impact of biofield. Pharm Anal Acta 6: 395.
Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Spectroscopic characterization of biofield treated metronidazole and tinidazole. Med Chem 5: 340-344.
Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Effect of biofield treatment on spectral properties of paracetamol and piroxicam. Chem Sci J 6: 98.
Trivedi MK, Branton A, Trivedi D, Shettigar H, Bairwa K, Jana S (2015) Fourier transform infrared and ultraviolet-visible spectroscopic characterization of biofield treated salicylic acid and sparfloxacin. Nat Prod Chem Res 3: 186.
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Morphological characterization, quality, yield and DNA fingerprinting of biofield energy treated alphonso mango (Mangifera indica L.). Journal of Food and Nutrition Sciences 3: 245-250.
Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015) Agronomic characteristics, growth analysis, and yield response of biofield treated mustard, cowpea, horse gram, and groundnuts. International Journal of Genetics and Genomics 3: 74-80.
Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015) Analysis of genetic diversity using simple sequence repeat (SSR) markers and growth regulator response in biofield treated cotton (Gossypium hirsutum L.). American Journal of Agriculture and Forestry 3: 216-221.
Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015) Evaluation of vegetative growth parameters in biofield treated bottle gourd (Lagenaria siceraria) and okra (Abelmoschus esculentus), International Journal of Nutrition and Food Sciences 4: 688-694.
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Evaluation of atomic, physical, and thermal properties of bismuth oxide powder: An impact of biofield energy treatment. American Journal of Nano Research and Applications 3: 94-98.
Trivedi MK, Patil S, Nayak G, Jana S, Latiyal O (2015) Influence of biofield treatment on physical, structural and spectral properties of boron nitride. J Material Sci Eng 4: 181.
Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O, Jana S (2015) Characterization of physical and structural properties of brass powder after biofield treatment. J Powder Metall Min 4: 134.
Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O, Jana S (2015) Evaluation of biofield treatment on physical and structural properties of bronze powder. Adv Automob Eng 4: 119.
Trivedi MK, Nayak G, Patil S, Tallapragada RM, Jana S, Mishra RK (2015) Bio-field treatment: An effective strategy to improve the quality of beef extract and meat infusion powder. J Nutr Food Sci 5: 389.
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Mishra RK, Jana S (2015) Biofield treatment: A potential strategy for modification of physical and thermal properties of gluten hydrolysate and ipomoea macroelements. J Nutr Food Sci 5: 414.
Czekanska EM, Stoddart MJ, Richards RG, Hayes JS (2012) In search of an osteoblast cell model for in vitro research. Eur Cells Mater 24: 1-17.
Biological evaluation of medical devices - Part 5: Tests for in vitro cytotoxicity (ISO 10993-5: 2009), I. S. EN ISO, 10993-5: 20093.
Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Meth 65: 55-63.
Marshall NJ, Goodwin CJ, Holt SJ (1995) A critical assessment of the use of microculture tetrazolium assays to measure cell growth and function. Growth Regul 5: 69-84.
Riss TL, Moravec RA, Niles AL, et al. Cell Viability Assays. 2013 May 1 [Updated 2016 Jul 1]. In: Sittampalam GS, Coussens NP, Brimacombe K, et al., editors. Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004.
Gerald J. Atkins, David M. Findlay, Paul H. Anderson, Howard A. Morris. Vitamin D (Third Edition), Vitamin D 2011, Pages 411–424. Volume I Chapter 23 – Target Genes: Bone Proteins.
Oishi Y, Fu ZW, Ohnuki Y, Kato H, Noguchi T (2002) Molecular basis of the alteration in skin collagen metabolism in response to in vivo dexamethasone treatment: Effects on the synthesis of collagen type I and III, collagenase, and tissue inhibitors of metalloproteinases. Br J Dermatol 147: 859-868.
Lips P, van Schoor NM (2011) The effect of vitamin D on bone and osteoporosis. Best Pract Res Clin Endocrinol Metab 25: 585-591.
Priemel M, von Domarus C, Klatte TO, Kessler S, Schlie J, Meier S, Proksch N, Pastor F, Netter C, Streichert T, Püschel K, Amling M (2010) Bone mineralization defects and vitamin D defciency: histomorphometric analysis of iliac crest bone biopsies and circulating 25-hydroxyvitamin D in 675 patients. J Bone Miner Res 25: 305-312.
Hossein-Nezhad A, Holick MF (2013) Vitamin D for health: A global perspective. Mayo Clin Proc 88: 720-755.