International Journal of Biochemistry, Biophysics & Molecular Biology

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Determination of Dynamic Viscosity in Samples of Blood Plasma and Hemoglobin Solution Using Nuclear Magnetic Resonance

Received: 11 December 2018    Accepted: 14 January 2019    Published: 10 January 2020
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Abstract

The viscosity evaluation in Plasma is extremely useful in the clinical evaluation of different diseases. A procedure is presented, based on Protonic Magnetic Resonance, for the evaluation of the dynamic viscosity in Blood Plasma and in hemoglobin solution from the determination of the transverse relaxation time (T2). To experimentally determine the T2 value, the impulse series Carr-Purcell-Meiboon-Gill was used in a MARAN DRX console (OXFORD INSTRUMENTS) and a homogeneous magnetic system (B0 = 0.095T). Values were obtained for the viscosity of the blood plasma and hemoglobin of 1.68 ± 0.12 mPas and 12.78 ± 3.55 mPas respectively, which agreed with the determined, in the same samples, using an Ostwald viscometer (1, 45 ± 0.06 mPas for the plasma and 12.82 ± 3.35 mPas for the dissolution of hemoglobin). The dynamic viscosity of the blood plasma was determined in 236 patients with Multiple Myeloma (2.19 ± 0.58 mPas), 142 with Drepanocytic Anemia (2.20 ± 0.79 mPas) showing statistically significant increases with respect to the characteristic values of the controls (1.68 ± 0.12mPas). Magnetic Relaxation is an option to evaluate plasma viscosity because it minimizes the volume of sample needed and eliminates the need to wash the viscometer between determinations. Magnetic Relaxation can compensate its relative high cost, compared with other Viscosimetry methods, facilitating other determinations of utility in several diseases.

DOI 10.11648/j.ijbbmb.20190402.12
Published in International Journal of Biochemistry, Biophysics & Molecular Biology (Volume 4, Issue 2, December 2019)
Page(s) 25-30
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Proton Magnetic Resonance, Dynamic Blood Plasma Viscosity and Hemoglobin

References
[1] Manuel A. Lores Guevara, Yulianela Mengana Torres, Juan C. García Naranjo, Norberto Rodríguez Suárez, Lidia C. Suáres Beyries, María A. Marichal Feliu, Teresa Simón Boada, Inocente C. Rodríguez Reyes, Jan Phillipé. Plasma Dynamic Viscosity Determined by NMR. Applied Magnetic Resonance 2018. DOI 10.1007/s00723-018-1026.
[2] Mette Harreby, et all. Viscosity of Plasma in patients with rheumatoid arthritis. Annals of the rheumatic DiseasesAugust, 1987, 46 (8) 601-604. ISSN: 0003-4967.
[3] Ajayi OI, Famodu AA, Oviasu E. Fibrinogen concentration. A marker of cardiovascular disorders in Nigerians. Turk J. Hematol; 2007, 24 (1), 18-22.
[4] Rosencranz, R. and Steven A. Clinical Laboratory Measurement of Serum. Plasma and Blood Viscosity. Clin. Pathol; 2006, 125 (1), 78-86. ISSN: 0974-7532.
[5] Recommendation for a selected method for the measurement of plasma viscosity. Clin. Pathol. 1984, 37 (10), 1147-1152.
[6] A. Shames, Y. Rozenfeld, D. Keinius, Apparatus and method for non-invasive measurement of blood parameters, Patent application number: 2015/0018638A1, Publication date: 15 de Enero del 2015.
[7] P.S. Wellman, M. Blanco, W. Peine, Portable device for nmr based analysis of rheological changes in liquid samples, Patent application number: US 2015/0130463A1, Publication date: may15, 2015.
[8] Lee Josephson, Rui Hong, Michael J. Cima, Ralph Weissleder, Magnetic resonance-based viscometers and methods, Patent application number: WO 2009/026164A1, Publication date: 26 de Febrero de 2009.
[9] Carlos Cabal Mirabal, Adolfo Fernández García, Manuel Lores Guevara, Evelio González, Leonardo Oramas Díaz. Kinetics studies of complex biomedical process by Magnetic Resonance. Cuban experiences. Applied Magnetic Resonance. https://doi.org/10.1007/s00723-018-0985-2
[10] R. S. Menon and P. S. Allen. Solventprotonrelaxation of aqueoussolutions of theserumproteinsα2-macroglobulin, fibrinogen and albumin. Biophysical Journal. 1990, 57 (3), 389-396. doi: 10.1016/S0006-3495(90)82555-8.
[11] M. Lores and C. Cabal. Proton Magnetic Relaxation Process during the Polymerization of Hemoglobin S. Appl. Magn.Reson. 2005, 28 (1), 79-84.
[12] http://scielo.isciii.es/pdf/fh/v39n3/02articuloespecial01.pdf, revisado en marzo 2018.
[13] Sánchez Hernández, B. E. Adolfo López Mateos, Viscosímetro de Bola, Tesis de Licenciatura de Química. Instituto Politécnico Nacional, México. D. F. 2008.
[14] Galego Fernández, N. Manual de prácticas de laboratorio de Química Física de las Superficies y Polímeros. Editorial Pueblo y Educación, La Habana. página 48. 1986.
[15] Lores Guevara, M. A. Estudios preliminares sobre el empleo de los radicales, en seguimiento de la Polimerización de la HbS. Tesis en opción del título de Ingeniero en Física Nuclear. Instituto Superior de Ciencias y tecnología Nuclear. 1993.
[16] Guerra Bustillo C. W., Menéndez Acuña E., Barrera Morera R. y Egaña Morales E. Estadística. Editorial Pueblo y Educación. 1989.
[17] www.quimica.urv.es/quimio/general/callin.pdf, revisado mayo 2018.
[18] Lores Guevara, M. A. 2005. Estudio de los procesos de interacción Magnética y la movilidad molecular durante la polimerización de la HbS con métodos de Resonancia Magnética. Tesis presentada en opción al título de Doctor en Ciencias Físicas. Universidad de Oriente. Cuba.
Author Information
  • Centro de Biofísica Médica, Universidad de Oriente, Patricio Lumumba S/N. Santiago de Cuba. Cuba

  • Centro de Biofísica Médica, Universidad de Oriente, Patricio Lumumba S/N. Santiago de Cuba. Cuba

  • Centro de Biofísica Médica, Universidad de Oriente, Patricio Lumumba S/N. Santiago de Cuba. Cuba

  • Centro de Biofísica Médica, Universidad de Oriente, Patricio Lumumba S/N. Santiago de Cuba. Cuba

  • Hospital General “Dr. Juan Bruno Zayas Alfonso”, Carretera del Caney S/N, Santiago de Cuba. Cuba

  • Hospital General “Dr. Juan Bruno Zayas Alfonso”, Carretera del Caney S/N, Santiago de Cuba. Cuba

  • Hospital General “Dr. Juan Bruno Zayas Alfonso”, Carretera del Caney S/N, Santiago de Cuba. Cuba

  • Hospital General “Dr. Juan Bruno Zayas Alfonso”, Carretera del Caney S/N, Santiago de Cuba. Cuba

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    Yulianela Mengana Torres, Manuel Arsenio Lores Guevara, Juan Carlos García Naranjo, Beatriz Taimy Ricardo Ferro, Lidia Clara Suárez Beyries, et al. (2020). Determination of Dynamic Viscosity in Samples of Blood Plasma and Hemoglobin Solution Using Nuclear Magnetic Resonance. International Journal of Biochemistry, Biophysics & Molecular Biology, 4(2), 25-30. https://doi.org/10.11648/j.ijbbmb.20190402.12

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    ACS Style

    Yulianela Mengana Torres; Manuel Arsenio Lores Guevara; Juan Carlos García Naranjo; Beatriz Taimy Ricardo Ferro; Lidia Clara Suárez Beyries, et al. Determination of Dynamic Viscosity in Samples of Blood Plasma and Hemoglobin Solution Using Nuclear Magnetic Resonance. Int. J. Biochem. Biophys. Mol. Biol. 2020, 4(2), 25-30. doi: 10.11648/j.ijbbmb.20190402.12

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    AMA Style

    Yulianela Mengana Torres, Manuel Arsenio Lores Guevara, Juan Carlos García Naranjo, Beatriz Taimy Ricardo Ferro, Lidia Clara Suárez Beyries, et al. Determination of Dynamic Viscosity in Samples of Blood Plasma and Hemoglobin Solution Using Nuclear Magnetic Resonance. Int J Biochem Biophys Mol Biol. 2020;4(2):25-30. doi: 10.11648/j.ijbbmb.20190402.12

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  • @article{10.11648/j.ijbbmb.20190402.12,
      author = {Yulianela Mengana Torres and Manuel Arsenio Lores Guevara and Juan Carlos García Naranjo and Beatriz Taimy Ricardo Ferro and Lidia Clara Suárez Beyries and Inocente Clemente Rodríguez Reyes and María A. Marichal Feliu and Yomaidis Araujo Durán},
      title = {Determination of Dynamic Viscosity in Samples of Blood Plasma and Hemoglobin Solution Using Nuclear Magnetic Resonance},
      journal = {International Journal of Biochemistry, Biophysics & Molecular Biology},
      volume = {4},
      number = {2},
      pages = {25-30},
      doi = {10.11648/j.ijbbmb.20190402.12},
      url = {https://doi.org/10.11648/j.ijbbmb.20190402.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijbbmb.20190402.12},
      abstract = {The viscosity evaluation in Plasma is extremely useful in the clinical evaluation of different diseases. A procedure is presented, based on Protonic Magnetic Resonance, for the evaluation of the dynamic viscosity in Blood Plasma and in hemoglobin solution from the determination of the transverse relaxation time (T2). To experimentally determine the T2 value, the impulse series Carr-Purcell-Meiboon-Gill was used in a MARAN DRX console (OXFORD INSTRUMENTS) and a homogeneous magnetic system (B0 = 0.095T). Values were obtained for the viscosity of the blood plasma and hemoglobin of 1.68 ± 0.12 mPas and 12.78 ± 3.55 mPas respectively, which agreed with the determined, in the same samples, using an Ostwald viscometer (1, 45 ± 0.06 mPas for the plasma and 12.82 ± 3.35 mPas for the dissolution of hemoglobin). The dynamic viscosity of the blood plasma was determined in 236 patients with Multiple Myeloma (2.19 ± 0.58 mPas), 142 with Drepanocytic Anemia (2.20 ± 0.79 mPas) showing statistically significant increases with respect to the characteristic values of the controls (1.68 ± 0.12mPas). Magnetic Relaxation is an option to evaluate plasma viscosity because it minimizes the volume of sample needed and eliminates the need to wash the viscometer between determinations. Magnetic Relaxation can compensate its relative high cost, compared with other Viscosimetry methods, facilitating other determinations of utility in several diseases.},
     year = {2020}
    }
    

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    T1  - Determination of Dynamic Viscosity in Samples of Blood Plasma and Hemoglobin Solution Using Nuclear Magnetic Resonance
    AU  - Yulianela Mengana Torres
    AU  - Manuel Arsenio Lores Guevara
    AU  - Juan Carlos García Naranjo
    AU  - Beatriz Taimy Ricardo Ferro
    AU  - Lidia Clara Suárez Beyries
    AU  - Inocente Clemente Rodríguez Reyes
    AU  - María A. Marichal Feliu
    AU  - Yomaidis Araujo Durán
    Y1  - 2020/01/10
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    DO  - 10.11648/j.ijbbmb.20190402.12
    T2  - International Journal of Biochemistry, Biophysics & Molecular Biology
    JF  - International Journal of Biochemistry, Biophysics & Molecular Biology
    JO  - International Journal of Biochemistry, Biophysics & Molecular Biology
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    EP  - 30
    PB  - Science Publishing Group
    SN  - 2575-5862
    UR  - https://doi.org/10.11648/j.ijbbmb.20190402.12
    AB  - The viscosity evaluation in Plasma is extremely useful in the clinical evaluation of different diseases. A procedure is presented, based on Protonic Magnetic Resonance, for the evaluation of the dynamic viscosity in Blood Plasma and in hemoglobin solution from the determination of the transverse relaxation time (T2). To experimentally determine the T2 value, the impulse series Carr-Purcell-Meiboon-Gill was used in a MARAN DRX console (OXFORD INSTRUMENTS) and a homogeneous magnetic system (B0 = 0.095T). Values were obtained for the viscosity of the blood plasma and hemoglobin of 1.68 ± 0.12 mPas and 12.78 ± 3.55 mPas respectively, which agreed with the determined, in the same samples, using an Ostwald viscometer (1, 45 ± 0.06 mPas for the plasma and 12.82 ± 3.35 mPas for the dissolution of hemoglobin). The dynamic viscosity of the blood plasma was determined in 236 patients with Multiple Myeloma (2.19 ± 0.58 mPas), 142 with Drepanocytic Anemia (2.20 ± 0.79 mPas) showing statistically significant increases with respect to the characteristic values of the controls (1.68 ± 0.12mPas). Magnetic Relaxation is an option to evaluate plasma viscosity because it minimizes the volume of sample needed and eliminates the need to wash the viscometer between determinations. Magnetic Relaxation can compensate its relative high cost, compared with other Viscosimetry methods, facilitating other determinations of utility in several diseases.
    VL  - 4
    IS  - 2
    ER  - 

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