Background: Shigellosis and salmonellosis are still global health problems, especially, in developing countries where poor sanitation, lack of clean water supply and proper sewage disposal system exist. The emergence of increased antimicrobial resistance of Shigella and Salmonella species are global challenges, particularly in developing countries like Ethiopia where increased misuse of antimicrobial agents by human beings occur. Objectives: To determine the prevalence and antimicrobial susceptibility patterns of Shigella and Salmonella isolates from patients with diarrhea attending the health institutions in Gondar town, Northwest Ethiopia. Materials and Methods: Health institutional based cross sectional study was carried out on diarrheic patients attending Gondar town health institutions, February 29, 2014 to May 20, 2014. Systematic random sampling technique was used and stool samples were collected from 372 study subjects. Samples were cultured onto MacConkey and Salmonella-Shigella agars and drug susceptibility patterns of the isolates were determined following standard bacteriological method. Data were coded and entered for statistical analysis using SPSS version 20. Data were presented using tables and chi-square. Result: Of the total of 372 stool cultures, 17(4.57%) Shigella spp. and 4(1.08%) Salmonella spp. were isolated. Most commonly isolated strains of Shigella were S. flexneri 11(64.7%) followed by S. dysenteriae 3(17.65%), S. boydii 2(11.77%) and S. sonnei 1(5.88%). Shigella isolates presented high resistance rate to ampicilin (94.1%), amoxicillin (88.2), tetracycline (88.2%) and Salmonella species were highly resistance to tetracycline (100%), amoxicillin (100%), and ampicillin (75%). However, all isolates of Shigella and Salmonella were 100% susceptible to ciprofloxacin and norfloxacin. Conclusion and recommendation: This study revealed that isolates of Shigella and Salmonella showed high rate of drug resistance to the commonly used antibiotics. However, all the isolates were susceptible to ciprofloxacin, and norfloxacin. Therefore, ciprofloxacin and norfloxacin can be used as drugs of choice for the treatment of Shigellosis and Salmonellosis.
| DOI | 10.11648/j.sjph.20140205.24 |
| Published in | Science Journal of Public Health (Volume 2, Issue 5, September 2014) |
| Page(s) | 469-475 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Antibiotic Susceptibility, Prevalence, Salmonella and Shigella
| [1] | Robert F AB. Basic Medical Microbiology. 5th ed. Little,brown and compny.1995:345-362. |
| [2] | Arias CA, Murray BE. Antibiotic-resistant bugs in the 21st century. A clinical super challenge. N Eng J Med. 2009;360(5):439-43. |
| [3] | WHO.Weekly Epidemiological report:Shigellosis:Evidence for prevention. A Publication of the Epidemiological Unit, Sri Lanka. 2007; 34, 1-3. |
| [4] | Central Statistical Agency Ethiopia and ICF International. Ethiopia Demographic and Health Survey 2011. Addis Ababa, Ethiopia and Calverton, Maryland, USA: Central Statistical Agency and ICF International. 2012. |
| [5] | Coburn B, Grassl GA, Finlay B. Salmonella, the host and disease: a brief review. Immu Cell biol. 2006;85(2):112-8. |
| [6] | Kumar Y, Sharma A, Sehgal R, Kumar S. Distribution trends of Salmonella serovars in India (2001–2005). Trans Royal Soc Trop Med Hyg. 2009;103(4):390-4. |
| [7] | Huruy K, Kassu A, Mulu A, Gebretsadik S, Andargie G, Tadesse T, Birhan W, Worku N, Ghebreselassie D, Belyhun Y, Yifru S, Adugna S, Tiruneh M. High level of antimicrobial resistance in Shigella Species isolated from diarrhoeal patients in University of Gondar teaching Hospital, Gondar, Ethiopia. Pharmaonline. 2008;2:328-340 |
| [8] | Birhaneselassie M, Williams D. A study of Salmonella carriage among asymptomatic food-handlers in southern Ethiopia. Inter J Nutr Food Sci. 2013; 2(5):243-245. |
| [9] | Gu B, Cao Y, Pan S, Zhuang L, Yu R, Peng Z, Qian H, Wei Y, Zhao L, Liu G, Tonq M. Comparison of the prevalence and changing resistance to nalidixic acid and ciprofloxacin of Shigella between Europe–America and Asia–Africa from 1998 to 2009. Inter J Antimicro. 2012;40(1):9-17. |
| [10] | Sharma R, Sharma C, Kapoor B. Antibacterial resistance: current problems and possible solutions. Indian J Med Sci. 2005;59(3):120. |
| [11] | Cheesbrough M. Medical Laboratory Manual for Tropical Countries. Vol. II: Microbiology. Butterworth-Heinemann Ltd, England. 2009:225-431. |
| [12] | Huruy K, Kassu A, Mulu A, Worku N, Fetene T, Gebretsadik S, Biadglegne F, Belyhun F, Muche A, Gelaw A, Anagaw A, Yifru S, Wondie Y, Bekele A, Tiruneh M, Reissig D, Moges F.Intestinal parasitosis and shigellosis among diarrheal patients in Gondar teaching hospital, Northwest Ethiopia. BMC Res Notes. 2011;4(1):472. |
| [13] | Asrat D. Shigella and Salmonella serogroups and their antibiotic susceptibility patterns in Ethiopia. East Mediterr Health J. 2008;14(4):760-7. |
| [14] | Beyene G, Asrat D, Mengistu Y, Aseffa A, Wain J. Typhoid fever in Ethiopia. journal of infection in developing countries. 2008;2(6). |
| [15] | Braude. A. Antimicrobial drug therapy,Major problems in internal medicine. New York, WB Saunders. 1976;8:111-218. |
| [16] | Ericsson CD, DuPont HL, Mathewson JJ, West MS, Johnson PC, Bitsura JAM. Treatment of traveler's diarrhea with sulfamethoxazole and trimethoprim and loperamide. Jama. 1990;263(2):257-61. |
| [17] | Harnett N, McLeod S, AuYong Y, Krishnan C. Increasing incidence of resistance among shigellae to trimethoprim. The Lancet. 1991;337(8741):622. |
| [18] | Commission FDRoEPC. Summary and statistical report of the 2007 population and housing census–population size by age and sex. Addis Ababa D. |
| [19] | Beyene G, Tasew H. Prevalence of intestinal parasite, Shigella and Salmonella species among diarrheal children in Jimma health center, Jimma southwest Ethiopia: a cross sectional study. Annals cli micro antim. 2014;13(1):10. |
| [20] | Perilla MJ, Ajello G, Bopp C, Elliott J, Facklam R, Popovic T, Wellys Jet. Manual for the Laboratory Identification and Antimicrobial Susceptibility Testing of Bacterial Pathogens of Public Health Importance in the Developing World. Centers for Disease Control and Prevention, National Center for Infectious Diseases.2003:1-313. |
| [21] | Clinical and labratory standard institute. performance standards for antimicrobila suceptibiltiy testing: tweenty third informational supplement. CLSI document M100-S23.clinical and labratory standard institute wvrw, USA.2013. |
| [22] | Herwana E, Surjawidjaja JE, Salim OC, Indriani N, Bukitwetan P, Lesmana M. Shigella associated diarrhea in children in South Jakarta, Indonesia. Southeast Asian J Trop Med Pub Health. 2010;41(2):418-25. |
| [23] | Orrett FA. Prevalence of Shigella serogroups and their antimicrobial resistance patterns in Southern Trinidad. J Health Pop Nutr. 2008;26(4):456. |
| [24] | Mardaneh J, Poor SA, Afrugh P. Prevalence of Shigella species and Antimicrobial Resistance Patterns of Isolated Strains from Infected Pediatrics in Tehran. Int J Entric Patho. 2013;1(1) |
| [25] | Brooks JT, Ochieng JB, Kumar L, Okoth G, Shapiro RL, Wells JG. Surveillance for bacterial diarrhea and antimicrobial resistance in rural western Kenya, 1997–2003. Clinical infectious diseases. 2006;43(4):393-401. |
| [26] | Brooks JT, Shapiro RL, Kumar L, Wells JG, Phillips-Howard PA, Shi Y-P, Vulule JM, Hoekstra RM, Mintze E, Slutsker L. Epidemiology of sporadic bloody diarrhea in rural Western Kenya. Am J Trop Med Hyg. 2003;68(6):671-7. |
| [27] | Mache A. Antibiotic resistance and sero-groups of Shigella among paediatric out-patients in Southern Ethiopia. E Afr Med J. 2001;78(6):296-9. |
| [28] | Roma B, Worku S, Mariam ST, Langeland N. Antimicrobial susceptibility pattern of Shigella isolates in Awassa. Ethiop J Health Dev. 2000;14(2):149-54. |
| [29] | Reda AA, Seyoum B, Yimam J, Andualem G, Fiseha S, Vandeweerd J-M. Antibiotic susceptibility patterns of Salmonella and Shigella isolates in Harar, Eastern Ethiopia. J Infect Dis Immun. 2011;3(8):134-9. |
| [30] | Yismaw O, Negeri C, Kassu A. A five-year antimicrobial resistance pattern observed in Shigella species isolated from stool samples in Gondar University Hospital, Northwest Ethiopia. Ethiop J Health Dev. 2006;20(3). |
| [31] | Tiruneh M. Serodiversity and antimicrobial resistance pattern of Shigella isolates at Gondar University teaching hospital, Northwest Ethiopia. Jpn J Infect Dis 2008;62(2):93-7. |
| [32] | Moges D. Prevalence of Salmonella and Shigella among Food Handlers in Catering Establishments in Hawassa University, Hawassa, Ethiopia. Master thesis. 2010.16-22 |
| [33] | Mache A. Antibiotic resistance and sero-groups of Shigella among paediatric out-patients in Southern Ethiopia. E Afr Med J. 2001;78(6):296-9. |
| [34] | Temu M, Kaatano G, Miyaye N, Buhalata S, Shushu M, Kishamawe C,Changalucha JM. Antimicrobial susceptibility of Shigella flexneri and S. dysenteriae isolated from stool specimens of patients with bloody diarrhoea in Mwansa, Tanzania. Tanza J Health Res. 2008;9(3):186-9. |
| [35] | Getamesay M, Getenet B, Ahmed Z. Prevalence of Shigella, Salmonella and Campylobacter Species and Their Susceptibility Patters Among Under Five Children With Diarrhea in Hawassa Town, South Ethiopia. Ethiop health sci. 2014;24(2):101-8. |
| [36] | Andargie G, Kassu A, Moges F, Tiruneh M, Huruy K. Prevalence of bacteria and intestinal parasites among food-handlers in Gondar town, Northwest Ethiopia. J Health Pop Nutr 2008;26(4):451 |
| [37] | Hamer D, Simon J, Thea D, Keusch G, Hernandez-Avila M, Lazcano-Ponce E. Childhood diarrhea in sub-Saharan Africa. Inter J Epi. 1998;27(3):370-6. |
| [38] | Savadkoohi RB, Ahmadpour-Kacho M. Prevalence of Shigella species and their antimicrobial resistance patterns at Amirkola children hospital, North of Iran. Iran J Ped 2007;17(2).118-122 |
APA Style
Tesfaye Andualem Demissie, Moges Tiruneh Wubie, Feleke Moges Yehuala, Dagnachew Muluye Fetene, Getnet Ayalew Gudeta. (2014). Prevalence and Antimicrobial Susceptibility Patterns of Shigella and Salmonella Species among Patients with Diarrhea Attending Gondar Town Health Institutions, Northwest Ethiopia. Science Journal of Public Health, 2(5), 469-475. https://doi.org/10.11648/j.sjph.20140205.24
ACS Style
Tesfaye Andualem Demissie; Moges Tiruneh Wubie; Feleke Moges Yehuala; Dagnachew Muluye Fetene; Getnet Ayalew Gudeta. Prevalence and Antimicrobial Susceptibility Patterns of Shigella and Salmonella Species among Patients with Diarrhea Attending Gondar Town Health Institutions, Northwest Ethiopia. Sci. J. Public Health 2014, 2(5), 469-475. doi: 10.11648/j.sjph.20140205.24
AMA Style
Tesfaye Andualem Demissie, Moges Tiruneh Wubie, Feleke Moges Yehuala, Dagnachew Muluye Fetene, Getnet Ayalew Gudeta. Prevalence and Antimicrobial Susceptibility Patterns of Shigella and Salmonella Species among Patients with Diarrhea Attending Gondar Town Health Institutions, Northwest Ethiopia. Sci J Public Health. 2014;2(5):469-475. doi: 10.11648/j.sjph.20140205.24
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Download@article{10.11648/j.sjph.20140205.24,
author = {Tesfaye Andualem Demissie and Moges Tiruneh Wubie and Feleke Moges Yehuala and Dagnachew Muluye Fetene and Getnet Ayalew Gudeta},
title = {Prevalence and Antimicrobial Susceptibility Patterns of Shigella and Salmonella Species among Patients with Diarrhea Attending Gondar Town Health Institutions, Northwest Ethiopia},
journal = {Science Journal of Public Health},
volume = {2},
number = {5},
pages = {469-475},
doi = {10.11648/j.sjph.20140205.24},
url = {https://doi.org/10.11648/j.sjph.20140205.24},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.20140205.24},
abstract = {Background: Shigellosis and salmonellosis are still global health problems, especially, in developing countries where poor sanitation, lack of clean water supply and proper sewage disposal system exist. The emergence of increased antimicrobial resistance of Shigella and Salmonella species are global challenges, particularly in developing countries like Ethiopia where increased misuse of antimicrobial agents by human beings occur. Objectives: To determine the prevalence and antimicrobial susceptibility patterns of Shigella and Salmonella isolates from patients with diarrhea attending the health institutions in Gondar town, Northwest Ethiopia. Materials and Methods: Health institutional based cross sectional study was carried out on diarrheic patients attending Gondar town health institutions, February 29, 2014 to May 20, 2014. Systematic random sampling technique was used and stool samples were collected from 372 study subjects. Samples were cultured onto MacConkey and Salmonella-Shigella agars and drug susceptibility patterns of the isolates were determined following standard bacteriological method. Data were coded and entered for statistical analysis using SPSS version 20. Data were presented using tables and chi-square. Result: Of the total of 372 stool cultures, 17(4.57%) Shigella spp. and 4(1.08%) Salmonella spp. were isolated. Most commonly isolated strains of Shigella were S. flexneri 11(64.7%) followed by S. dysenteriae 3(17.65%), S. boydii 2(11.77%) and S. sonnei 1(5.88%). Shigella isolates presented high resistance rate to ampicilin (94.1%), amoxicillin (88.2), tetracycline (88.2%) and Salmonella species were highly resistance to tetracycline (100%), amoxicillin (100%), and ampicillin (75%). However, all isolates of Shigella and Salmonella were 100% susceptible to ciprofloxacin and norfloxacin. Conclusion and recommendation: This study revealed that isolates of Shigella and Salmonella showed high rate of drug resistance to the commonly used antibiotics. However, all the isolates were susceptible to ciprofloxacin, and norfloxacin. Therefore, ciprofloxacin and norfloxacin can be used as drugs of choice for the treatment of Shigellosis and Salmonellosis.},
year = {2014}
}
TY - JOUR T1 - Prevalence and Antimicrobial Susceptibility Patterns of Shigella and Salmonella Species among Patients with Diarrhea Attending Gondar Town Health Institutions, Northwest Ethiopia AU - Tesfaye Andualem Demissie AU - Moges Tiruneh Wubie AU - Feleke Moges Yehuala AU - Dagnachew Muluye Fetene AU - Getnet Ayalew Gudeta Y1 - 2014/09/20 PY - 2014 N1 - https://doi.org/10.11648/j.sjph.20140205.24 DO - 10.11648/j.sjph.20140205.24 T2 - Science Journal of Public Health JF - Science Journal of Public Health JO - Science Journal of Public Health SP - 469 EP - 475 PB - Science Publishing Group SN - 2328-7950 UR - https://doi.org/10.11648/j.sjph.20140205.24 AB - Background: Shigellosis and salmonellosis are still global health problems, especially, in developing countries where poor sanitation, lack of clean water supply and proper sewage disposal system exist. The emergence of increased antimicrobial resistance of Shigella and Salmonella species are global challenges, particularly in developing countries like Ethiopia where increased misuse of antimicrobial agents by human beings occur. Objectives: To determine the prevalence and antimicrobial susceptibility patterns of Shigella and Salmonella isolates from patients with diarrhea attending the health institutions in Gondar town, Northwest Ethiopia. Materials and Methods: Health institutional based cross sectional study was carried out on diarrheic patients attending Gondar town health institutions, February 29, 2014 to May 20, 2014. Systematic random sampling technique was used and stool samples were collected from 372 study subjects. Samples were cultured onto MacConkey and Salmonella-Shigella agars and drug susceptibility patterns of the isolates were determined following standard bacteriological method. Data were coded and entered for statistical analysis using SPSS version 20. Data were presented using tables and chi-square. Result: Of the total of 372 stool cultures, 17(4.57%) Shigella spp. and 4(1.08%) Salmonella spp. were isolated. Most commonly isolated strains of Shigella were S. flexneri 11(64.7%) followed by S. dysenteriae 3(17.65%), S. boydii 2(11.77%) and S. sonnei 1(5.88%). Shigella isolates presented high resistance rate to ampicilin (94.1%), amoxicillin (88.2), tetracycline (88.2%) and Salmonella species were highly resistance to tetracycline (100%), amoxicillin (100%), and ampicillin (75%). However, all isolates of Shigella and Salmonella were 100% susceptible to ciprofloxacin and norfloxacin. Conclusion and recommendation: This study revealed that isolates of Shigella and Salmonella showed high rate of drug resistance to the commonly used antibiotics. However, all the isolates were susceptible to ciprofloxacin, and norfloxacin. Therefore, ciprofloxacin and norfloxacin can be used as drugs of choice for the treatment of Shigellosis and Salmonellosis. VL - 2 IS - 5 ER -
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