Epidemiology of Adenovirus and Genotypic Characteristics of Group A Rotavirus Among Diarrhoeic Children Aged 0 to 5 Years Old in the Ngaoundere Region of Cameroon
Science Journal of Public Health
Volume 3, Issue 4, July 2015, Pages: 525-537
Received: Apr. 13, 2015; Accepted: Apr. 29, 2015; Published: Jun. 6, 2015
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Authors
Kagning Tsinda Emmanuel, Department of Biomedical Sciences, University of Ngaoundere, Ngaoundere, Cameroon
Fokunang Charles Ntungwen, Department of Pharmacotoxicology and pharmacokinetics, University of Yaounde I, Yaounde, Cameroon
Tchuenguem Fohouo Fernand-Nestor, Department of Biological Sciences, University of Ngaoundere, Ngaoundere, Cameroon
Nwabo Kamdje Armel Herve, Department of Biomedical Sciences, University of Ngaoundere, Ngaoundere, Cameroon
Nukenine Elias Nchinwan, Department of Biological Sciences, University of Ngaoundere, Ngaoundere, Cameroon
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Abstract
In the context of recent introduction of a Rotavirus vaccine, little is known about the epidemiology of Rotavirus and Adenovirus gastroenteritis in Cameroon in general and in Ngaoundere specifically. The main objective of this study was to determine the frequency of Rotavirus and Adenovirus antigens as well as to characterise circulating Rotavirus strains in children with diarrhoea in Ngaoundere. A convenience sampling method was used and stool samples collected from patients consulting at the study sites from April to July 2014 were subjected to a qualitative immunochromatographic test to detect group antigens of group A Rotavirus and Adenovirus. Socio-demographic data were collected using a pre-tested questionnaire. Stool samples tested positive for Group A Rotavirus were genotyped by multiplex nested RT-PCR method. Logistic regression, Chi-square or Fisher’s exact tests were performed when appropriate. P-value < 0.05 at 95% confidence interval was considered statistically significant. Adenovirus antigen was detected in 26.4% (56) of children, whereas, group A Rotavirus was detected in 2.3% (5); the co-infection rate was 5.3%. Vomiting, fever, moderate dehydration, severe dehydration and the presence of polymorphonuclear cells in stool were significantly associated with the presence of Adenovirus antigen in stool. Group A Rotavirus was also found to circulate in Ngaoundere and the G1P[8] genotype showed indication of being the most represented. Adenovirus antigen was shown to be an important agent in childhood acute gastroenteritis in Ngaoundere and this study has been used as a baseline data for this infection in this region of Cameroon. The appropriate management of viral acute gastroenteritis necessitates the detection of Rotavirus and Adenovirus in clinical routine practice. Furthermore, Rotarix™ vaccination should be encouraged and the populations should be educated on hygiene practices.
Keywords
Rotavirus, Adenovirus, Epidemiology, Genotypes, Children Aged 0 to 5 Years
To cite this article
Kagning Tsinda Emmanuel, Fokunang Charles Ntungwen, Tchuenguem Fohouo Fernand-Nestor, Nwabo Kamdje Armel Herve, Nukenine Elias Nchinwan, Epidemiology of Adenovirus and Genotypic Characteristics of Group A Rotavirus Among Diarrhoeic Children Aged 0 to 5 Years Old in the Ngaoundere Region of Cameroon, Science Journal of Public Health. Vol. 3, No. 4, 2015, pp. 525-537. doi: 10.11648/j.sjph.20150304.21
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