Science Journal of Public Health
Volume 5, Issue 3, May 2017, Pages: 178-185
Received: Jan. 25, 2017;
Accepted: Feb. 10, 2017;
Published: Mar. 15, 2017
Views 1880 Downloads 151
Omoya O. F., Department of Microbiology, School of Sciences, Federal University of Technology, Akure, Nigeria
Momoh A. O., Department of Biological Sciences, Elizade University, Ilara-Mokin, Nigeria
Adegoke O. A., Department of Microbiology, School of Sciences, Federal University of Technology, Akure, Nigeria
Mushrooms are considered as a functional food, which can provide health benefits beyond the traditional nutrients they contain and Pleurotus ostreatus is a common edible mushroom known for its oyster-shaped cap. The in-vivo evaluation of the antiplasmodial property of ethanol and hot water extracts of P. ostreatus mushroom was carried out using standard microbiological methods. The mice were infected with Plasmodium berghei NK 65 species and the 4-day Peters suppressive test was carried out using 200 and 400 mg/ml of the extracts respectively. 5mg/ml chloroquine antimalarial drug was used as control drug. The malaria parasite load, full blood counts and histopathological analysis of the liver of the mice were done at the end of the experiment. The results showed that P. berghei caused a reduction in the weight from 22.8±0.20 g to 17.4±0.66 g. as well as a reduction in the temperature of the mice from 36.8±1.22°C to 34.2±0.20°C. The mushroom hot water extract exerted the highest percentage suppression of 76.82% after 24 hours, 92.35% after 48 hours, 96.08% after 72 hours and 99.81% after 96 hours. The parasite caused an increase in Erythrocyte Sedimentation Rate (ESR) from 1.33±0.33 mm/hr to 11.66±0.93 mm/hr, high white blood cell (WBC) count of 275.6 mm-3 and low packed cell volume (PCV) of 14.00±0.57%. The WBC differential count showed that the parasites caused a reduction in the number of lymphocytes (from 64.33% to 51.67%) while causing increase in neutrophils (25.33% to 32.67%). The P. berghei caused some negative pathological features ranging from a liver hepatocytes with profuse haemorrhage, dilated sinusoids and hepatocellular drainage, while the control group had well-formed and thickened liver hepatocytes without distinct cells or infiltrations. Therefore, Pleurotus ostreatus have antiplasmodial property and the hot water extract is more potent than the ethanol extract.
Omoya O. F.,
Momoh A. O.,
Adegoke O. A.,
In Vivo Evaluation of the Antiplasmodial Property of Ethanol and Hot Water Extracts of Pleurotus ostreatus Mushroom, Science Journal of Public Health.
Vol. 5, No. 3,
2017, pp. 178-185.
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/
) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Smith, J. E; Rowan, N. J. and sullivan, R. (2015). Medicinal mushroom: a rapidly developing area of biotechnology for cancer therapy and other bioactivities. Review Biotechnology letters. 7: (3) 55-64.
Kuo, M. (2006). Describing mushrooms and keeping a journal. Retrieved from the www. mushroomexpert.com.
Dandar A, Acy H and Yildiza A. (2008). Yield Performance and nutritional contents of three oyster mushroom species cultivated on wheat stalk. Afr J. biotechy. 7: 3497-3501.
Trudell, S; Ammirati, J. (2009). Mushrooms of the Pacific Northwest. Timber Press Field Guides. Port-land, Oregon: Timber Press. p. 134.
Phillips, Roger (2006), Mushrooms. Pub. McMillan,, ISBN 0-330-44237-6. P. 266.
World Health Organization, W. H. O. (2014). Prevention of Malaria Infections: A Practical Guide. 2nd edition. WHO/CDS/CSR/EPH/2014.12.
Fawole, M. A., and Oso, B. A. (2004): Laboratory Manual of Microbiology, Spectrum Books Limited, Ibadan, Nigeria. pp 127.
Funmilola O. Omoya and Abdul O. Momoh (2014). The Efficacy of Andrographis paniculata and Chromolena odorata plant extracts against Malaria parasite. International Journal of Biological, Life Science and Engineering, Vol: 8, No: 5, 134-137.
Baker, J. F., Breach, M. R. and Chris, P. (2010). Medical Laboratory Science. Chris Publisher, 2nd edition, United Kingdom, 487pp.
Chelsbrough. M. (2014). District Laboratory Practice, Tropical Countries; 2nd Edition, Cambridge University Press, United Kingdom. 480pp.
Oladunmoye, M. K.; Oso, B. I. and Momoh, A. O. (2014). Nutritional and immune-modulatory activities of extra virgin olive oil in the treatment of diarrhoea in albino rats infected with Escherichia coli 0157: H7. The journal of Agriculture and Natural Resources Sciences. Vol.: 1 (1). 45-56.
Nester, E. W.; Anderson, D. G.; Robert, C. E.; Pearsall, N. N. and Nester, M. T. (2014). Microbiology- A Human Perspective, 7th edition, McGraw Hill, New York, USA. Pp903.
Akindahunsi, A. A, and Oyetayo, F. L. (2012). Nutrient and anti-nutrient distribution of edible mushroom, Pleurotus tuber-regium (Fries) Singer. Food Science and Technology. v. 39, n. 5, p. 548-553.
Owolabi, B. J. and Olarinoye, N. O. (2010). Organic Chemistry; The Fundamentals. Bohan Press Ltd. Ibadan. 507pp.
Brooks, G. F.; Butel, J. S. and Morse, S. A. (2014). Medical Microbiology. 26th edition, McGraw Hill, New York, USA. Pp 826.
Al-faraj, S. (2009). Haemorrhagic colitis induced by citrulluscolcynthis. Annals of Tropical Medicine and Parasitology, 89, (6): 695-6.