Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients

Liu Guihua; Huang Xin; Zhang Weiyu; Wang Yanqiu

doi:doi:10.11648/j.sjph.20200803.13

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Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients

Liu Guihua, Huang Xin, Zhang Weiyu, Wang Yanqiu

Published in Science Journal of Public Health (Volume 8, Issue 3)

Received: 6 May 2020 Accepted: 22 May 2020 Published: 4 June 2020

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Abstract

Objective: To detect the drug resistance of Salmonella isolated from the faeces samples of patients with food-borne diseases, and to know the drug resistance status and development trend of food-borne pathogens in our province. Methods: Enrichment, color medium separation, API20E biochemistry and Thai serum Identification. The minimal inhibitory concentration (MIC) of Salmonella to 15 kinds of antibiotics were quantitatively determined by micro broth dilution method. Results: 123 food-borne Salmonella strains were resistant to ampicillin (AMP), NALIDIXIC acid (NAL), tetracycline (TET), trimethoprim/sulfamethoxazole (SXT), ampicillin / sulbactam (AMS), CEFAZOLIN (CFZ), chloramphenicol (CHL), CEFOTAXIME (CTX), ciprofloxacin (CIP), azithromycin (AZM), gentamicin (GEN), cefoxitin (CFX), ceftazidime (CAZ). The drug resistance rates were 87.80%, 84.6%, 69.1%, 50.4%, 39.0%, 34.1%, 26.0%, 26.0%, 26.0%, 24.3%, 19.5%, 2.4% and 0.8% respectively. IMP and ERY were both sensitive. Among them, 90 strains were resistant to more than 3 kinds of antibiotics, and the multidrug resistance rate was 73.2%. MIC results ranged from 1μg / mL to > 64 μg / mL. The total strains were divided into 15 serotypings, including Salmonella Enteritidis (58.5%), Salmonella Typhimurium (16.3%), and Salmonella Stanley (4.9%). The most common distribution of the 57 resistant strains were AMP-TET-NAL-and AMP-NAL-11 strains, followed by NAL-10 strains, AMP-AMS-TET-NAL-7 strains, and multi-resistant strains ranged from 3 to 11 antibiotics. Conclusion: The serotypings of Salmonella isolated from faeces samples of patients from food borne disease surveillance sites in hospitals in Jilin province were mainly S. Enteritis and S. Enterica, and the strains were resistant to multiple antibiotics. The surveillance of drug resistance of Salmonella in food-borne diseases should be strengthened to ensure food safety and human health.

Published in	Science Journal of Public Health (Volume 8, Issue 3)
DOI	10.11648/j.sjph.20200803.13
Page(s)	77-82
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Foodborne Diseases, Broth Dilution, MIC, Salmonella, Results Analysis

References

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APA Style

Liu Guihua, Huang Xin, Zhang Weiyu, Wang Yanqiu. (2020). Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients. Science Journal of Public Health, 8(3), 77-82. https://doi.org/10.11648/j.sjph.20200803.13

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ACS Style

Liu Guihua; Huang Xin; Zhang Weiyu; Wang Yanqiu. Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients. Sci. J. Public Health 2020, 8(3), 77-82. doi: 10.11648/j.sjph.20200803.13

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AMA Style

Liu Guihua, Huang Xin, Zhang Weiyu, Wang Yanqiu. Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients. Sci J Public Health. 2020;8(3):77-82. doi: 10.11648/j.sjph.20200803.13

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@article{10.11648/j.sjph.20200803.13,
  author = {Liu Guihua and Huang Xin and Zhang Weiyu and Wang Yanqiu},
  title = {Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients},
  journal = {Science Journal of Public Health},
  volume = {8},
  number = {3},
  pages = {77-82},
  doi = {10.11648/j.sjph.20200803.13},
  url = {https://doi.org/10.11648/j.sjph.20200803.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.20200803.13},
  abstract = {Objective: To detect the drug resistance of Salmonella isolated from the faeces samples of patients with food-borne diseases, and to know the drug resistance status and development trend of food-borne pathogens in our province. Methods: Enrichment, color medium separation, API20E biochemistry and Thai serum Identification. The minimal inhibitory concentration (MIC) of Salmonella to 15 kinds of antibiotics were quantitatively determined by micro broth dilution method. Results: 123 food-borne Salmonella strains were resistant to ampicillin (AMP), NALIDIXIC acid (NAL), tetracycline (TET), trimethoprim/sulfamethoxazole (SXT), ampicillin / sulbactam (AMS), CEFAZOLIN (CFZ), chloramphenicol (CHL), CEFOTAXIME (CTX), ciprofloxacin (CIP), azithromycin (AZM), gentamicin (GEN), cefoxitin (CFX), ceftazidime (CAZ). The drug resistance rates were 87.80%, 84.6%, 69.1%, 50.4%, 39.0%, 34.1%, 26.0%, 26.0%, 26.0%, 24.3%, 19.5%, 2.4% and 0.8% respectively. IMP and ERY were both sensitive. Among them, 90 strains were resistant to more than 3 kinds of antibiotics, and the multidrug resistance rate was 73.2%. MIC results ranged from 1μg / mL to > 64 μg / mL. The total strains were divided into 15 serotypings, including Salmonella Enteritidis (58.5%), Salmonella Typhimurium (16.3%), and Salmonella Stanley (4.9%). The most common distribution of the 57 resistant strains were AMP-TET-NAL-and AMP-NAL-11 strains, followed by NAL-10 strains, AMP-AMS-TET-NAL-7 strains, and multi-resistant strains ranged from 3 to 11 antibiotics. Conclusion: The serotypings of Salmonella isolated from faeces samples of patients from food borne disease surveillance sites in hospitals in Jilin province were mainly S. Enteritis and S. Enterica, and the strains were resistant to multiple antibiotics. The surveillance of drug resistance of Salmonella in food-borne diseases should be strengthened to ensure food safety and human health.},
 year = {2020}
}

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TY - JOUR
T1 - Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients
AU - Liu Guihua
AU - Huang Xin
AU - Zhang Weiyu
AU - Wang Yanqiu
Y1 - 2020/06/04
PY - 2020
N1 - https://doi.org/10.11648/j.sjph.20200803.13
DO - 10.11648/j.sjph.20200803.13
T2 - Science Journal of Public Health
JF - Science Journal of Public Health
JO - Science Journal of Public Health
SP - 77
EP - 82
PB - Science Publishing Group
SN - 2328-7950
UR - https://doi.org/10.11648/j.sjph.20200803.13
AB - Objective: To detect the drug resistance of Salmonella isolated from the faeces samples of patients with food-borne diseases, and to know the drug resistance status and development trend of food-borne pathogens in our province. Methods: Enrichment, color medium separation, API20E biochemistry and Thai serum Identification. The minimal inhibitory concentration (MIC) of Salmonella to 15 kinds of antibiotics were quantitatively determined by micro broth dilution method. Results: 123 food-borne Salmonella strains were resistant to ampicillin (AMP), NALIDIXIC acid (NAL), tetracycline (TET), trimethoprim/sulfamethoxazole (SXT), ampicillin / sulbactam (AMS), CEFAZOLIN (CFZ), chloramphenicol (CHL), CEFOTAXIME (CTX), ciprofloxacin (CIP), azithromycin (AZM), gentamicin (GEN), cefoxitin (CFX), ceftazidime (CAZ). The drug resistance rates were 87.80%, 84.6%, 69.1%, 50.4%, 39.0%, 34.1%, 26.0%, 26.0%, 26.0%, 24.3%, 19.5%, 2.4% and 0.8% respectively. IMP and ERY were both sensitive. Among them, 90 strains were resistant to more than 3 kinds of antibiotics, and the multidrug resistance rate was 73.2%. MIC results ranged from 1μg / mL to > 64 μg / mL. The total strains were divided into 15 serotypings, including Salmonella Enteritidis (58.5%), Salmonella Typhimurium (16.3%), and Salmonella Stanley (4.9%). The most common distribution of the 57 resistant strains were AMP-TET-NAL-and AMP-NAL-11 strains, followed by NAL-10 strains, AMP-AMS-TET-NAL-7 strains, and multi-resistant strains ranged from 3 to 11 antibiotics. Conclusion: The serotypings of Salmonella isolated from faeces samples of patients from food borne disease surveillance sites in hospitals in Jilin province were mainly S. Enteritis and S. Enterica, and the strains were resistant to multiple antibiotics. The surveillance of drug resistance of Salmonella in food-borne diseases should be strengthened to ensure food safety and human health.
VL - 8
IS - 3
ER -

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Author Information

Liu Guihua

Institute of Microbiology Department, Jilin Provincial Center for Disease Control and Prevention, Changchun, China
Huang Xin

Institute of Microbiology Department, Jilin Provincial Center for Disease Control and Prevention, Changchun, China
Zhang Weiyu

Institute of Microbiology Department, Jilin Provincial Center for Disease Control and Prevention, Changchun, China
Wang Yanqiu

Institute of Microbiology Department, Jilin Provincial Center for Disease Control and Prevention, Changchun, China

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Plain Text BibTeX RIS

APA Style

Liu Guihua, Huang Xin, Zhang Weiyu, Wang Yanqiu. (2020). Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients. Science Journal of Public Health, 8(3), 77-82. https://doi.org/10.11648/j.sjph.20200803.13

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ACS Style

Liu Guihua; Huang Xin; Zhang Weiyu; Wang Yanqiu. Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients. Sci. J. Public Health 2020, 8(3), 77-82. doi: 10.11648/j.sjph.20200803.13

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AMA Style

Liu Guihua, Huang Xin, Zhang Weiyu, Wang Yanqiu. Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients. Sci J Public Health. 2020;8(3):77-82. doi: 10.11648/j.sjph.20200803.13

Copy | Download

@article{10.11648/j.sjph.20200803.13,
  author = {Liu Guihua and Huang Xin and Zhang Weiyu and Wang Yanqiu},
  title = {Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients},
  journal = {Science Journal of Public Health},
  volume = {8},
  number = {3},
  pages = {77-82},
  doi = {10.11648/j.sjph.20200803.13},
  url = {https://doi.org/10.11648/j.sjph.20200803.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.20200803.13},
  abstract = {Objective: To detect the drug resistance of Salmonella isolated from the faeces samples of patients with food-borne diseases, and to know the drug resistance status and development trend of food-borne pathogens in our province. Methods: Enrichment, color medium separation, API20E biochemistry and Thai serum Identification. The minimal inhibitory concentration (MIC) of Salmonella to 15 kinds of antibiotics were quantitatively determined by micro broth dilution method. Results: 123 food-borne Salmonella strains were resistant to ampicillin (AMP), NALIDIXIC acid (NAL), tetracycline (TET), trimethoprim/sulfamethoxazole (SXT), ampicillin / sulbactam (AMS), CEFAZOLIN (CFZ), chloramphenicol (CHL), CEFOTAXIME (CTX), ciprofloxacin (CIP), azithromycin (AZM), gentamicin (GEN), cefoxitin (CFX), ceftazidime (CAZ). The drug resistance rates were 87.80%, 84.6%, 69.1%, 50.4%, 39.0%, 34.1%, 26.0%, 26.0%, 26.0%, 24.3%, 19.5%, 2.4% and 0.8% respectively. IMP and ERY were both sensitive. Among them, 90 strains were resistant to more than 3 kinds of antibiotics, and the multidrug resistance rate was 73.2%. MIC results ranged from 1μg / mL to > 64 μg / mL. The total strains were divided into 15 serotypings, including Salmonella Enteritidis (58.5%), Salmonella Typhimurium (16.3%), and Salmonella Stanley (4.9%). The most common distribution of the 57 resistant strains were AMP-TET-NAL-and AMP-NAL-11 strains, followed by NAL-10 strains, AMP-AMS-TET-NAL-7 strains, and multi-resistant strains ranged from 3 to 11 antibiotics. Conclusion: The serotypings of Salmonella isolated from faeces samples of patients from food borne disease surveillance sites in hospitals in Jilin province were mainly S. Enteritis and S. Enterica, and the strains were resistant to multiple antibiotics. The surveillance of drug resistance of Salmonella in food-borne diseases should be strengthened to ensure food safety and human health.},
 year = {2020}
}

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