Functional Status of Skin Fibroblasts at Chronic Staphylococcus Aureus Osteomyelitis of the Tibia
American Journal of Internal Medicine
Volume 1, Issue 2, July 2013, Pages: 10-13
Received: Jun. 16, 2013; Published: Jul. 10, 2013
Views 2954      Downloads 84
Authors
Igor Zhurakovsky, Central Research Laboratory, Novosibirsk State Medical University, Novosibirsk, Russia
Sergey Arkhypov, Central Research Laboratory, Novosibirsk State Medical University, Novosibirsk, Russia
Marya Pustovetova, Central Research Laboratory, Novosibirsk State Medical University, Novosibirsk, Russia
Igor Marinkin, Central Research Laboratory, Novosibirsk State Medical University, Novosibirsk, Russia
Tatyana Kunts, Central Research Laboratory, Novosibirsk State Medical University, Novosibirsk, Russia
Article Tools
PDF
Follow on us
Abstract
Inflammatory process is not limited only local effects but could affect other organs and tissues. In this study, we investigated the influence of experimental tibial osteomyelitis on skin cells in dynamics. Although the skin located at the distance from the infection nidus it supposed to manifest intensive proliferation, reparation and apoptosis under adverse factors. Immunohistochemical assay of apoptotic regulators and proliferation-related proteins was carried out in fibroblasts of stratum reticulare in Wistar rats. Proliferating fibroblasts pool decrease confirmed by Ki67 expression decrease was revealed, overexpression of p53 and Bax was accompanied by Bcl-2 expression decrease. Findings suggest depressed regeneratory potential of fibroblasts, high probability of apoptosis in the skin cells at the persisting osteomyelitis that should be considered in dermatological practice.
Keywords
Osteomyelitis, Tibia, Р53, Bax, Bcl-2, Ki-67, Fibroblasts, Stratum Reticulare, Apoptosis, Staphylococcus Аureus
To cite this article
Igor Zhurakovsky, Sergey Arkhypov, Marya Pustovetova, Igor Marinkin, Tatyana Kunts, Functional Status of Skin Fibroblasts at Chronic Staphylococcus Aureus Osteomyelitis of the Tibia, American Journal of Internal Medicine. Vol. 1, No. 2, 2013, pp. 10-13. doi: 10.11648/j.ajim.20130102.11
References
[1]
R. S. Akhtar, Y. Geng, B. J. Klocke, K. A. Roth, "Neural precursor cells possess multiple p53-dependent apoptotic pathways ", Cell Death Differ., 2006, vol. 13, pp. 1727-1739.
[2]
S. A. Arkhipov, V. A. Shkurupy, M. V. Solomatina, E. S. Akhramenko, D. A. Iljine, "Study of macrophages in BCG granulomas in different compartments of the mononuclear phagocyte system", Bull. Exp. Biol. Med., February 2013, vol. 154 (4), pp. 467-470.
[3]
F. Bibeau, F. Boissière-Michot, J. C. Sabourin, "Assessment of epidermal growth factor receptor (EGFR) expression in primary colorectal carcinomas and their related metastases on tissue sections and tissue microarray", Virchows Arch., 2006, vol. 449 (3), pp. 281-287.
[4]
О. V. Bukharin, "Significance of bacterial pathogens persistence for clinical practice", Russ. Med. News, 2000, vol. 5 (3), pp. 18-25.
[5]
C. Franke, M. Nöldner, R. Abdel-Kader, L. N. Johnson-Anuna, W. Gibson Wood, W. E. Müller, G. P. Eckert, "Bcl-2 upregulation and neuroprotection in guinea pig brain following chronic simvastatin treatment", Neurobiol. Dis., 2007, vol. 25 (2), pp. 438-445.
[6]
B. N. Green, C. D. Johnson, J. T. Egan, M. Rosenthal, E.A. Griffith, M. W. Evans, "Methicillin-resistant Staphylococcus aureus: an overview for manual therapists", J. Chiropr. Med., 2012, vol. 11 (1), pp. 64-76.
[7]
M. Jarvis, U. Schulz, A. M. Dickinson, L .Sviland, G. Jackson, A. Konur, X. N. Wang, I. Hromadnikova, H. J. Kolb, G. Eissner, E. Holler, "The detection of apoptosis in a human in vitro skin explant assay for graft versus host reactions", J. Clin. Pathol., Feb 2002, vol. 55, pp. 127-132.
[8]
S. L. Harris, A. J. Levine, "The p53 pathway: positive and negative feedback loops", Oncogene, 2005, vol. 24. pp. 2899-2908.
[9]
V. A. Karpin, "General pathology: chronic infectious process", Succes. Nat. Sс., 2005, vol. 4, pp. 17- 20.
[10]
P. J. Lindsberg, A. J. Grau "Inflammation and infections as risk factors for ichemic stroke", Stroke, 2003, Oct, vol. 34, pp. 2518-2532.
[11]
Mathieu, L. M. Cruz-Orive, H. Hoppeler, E. R. Weibel "Measuring error and sampling variation in stereology: Comparison of the efficiency of various methods of planar image analysis", J. Microsc., 1981, vol. 121 (1). pp. 75-88.
[12]
T. J. Pallasch, M. J. Wahl, "The focal infection theory: appraisal and reappraisal", J. Calif. Dent. Ass., 2000, vol. 28 (3), pp. 194-200.
[13]
N. A. Pecareva, L. A. Trunova, T. V. Belousova, O. M. Gorbenko, A. P. Shvayuk, A. N. Trunov, "About activity of immune-inflammation in children with chronic pyelonephritis in clinical remission", Bull. Russ. Acad. Med Sc., 2008, vol. 131 (3), pp. 52-55.
[14]
J. A. Ramos-Vara, "Technical Aspects of Immunohistochemistry", Vet. Pathol., 2005, vol. 42 (4), pp. 405-426.
[15]
M. Schuler, D. R. Green, "Transcription, apoptosis and p53: Catch- 22", Trends Genet., 2005, vol. 21, pp. 182-187.
[16]
J. Yu, L. Zhang, "The transcriptional targets of p53 in apoptosis control", Biochem. Biophys. Res. Commun., 2005, vol. 331, pp. 851-858.
[17]
P. Zhurakovsky, S. A. Arkhipov, M. G. Pustovetova , T. A. Kunts, M. V. Bitkhaeva, I. O. Marinkin, "Activation of mitochondrial way hepatocyte apoptosis when the persistence of bacterial infection", Zabaik. Med Bull., 2011, vol. 2, pp. 125-131.
[18]
P. Zhurakovsky, S. A. Arkhipov, M. G. Pustovetova , T. A. Kunts, M. V. Bitkhaeva, I. O. Marinkin, "Bcl-2 expression changes in rat liver and cytokines levels in blood serum at the persistence of a bacterial infection", Kuban. Med. Sc. Bull., 2012, vol. 2, pp. 84-88.
ADDRESS
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
U.S.A.
Tel: (001)347-983-5186