Expression of NF-κB and Occludin in Intestinal Mucosal Barrier During Severe Acute Pancreatitis and Its Clinical Significance
American Journal of Internal Medicine
Volume 5, Issue 5, September 2017, Pages: 70-73
Received: Jul. 5, 2017; Accepted: Jul. 14, 2017; Published: Aug. 7, 2017
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Authors
Qingyun Zhu, Department of Intensive Care Unit, The Affiliated Hospital of Qingdao University, Qingdao, China
Shigang Yang, Department of Gastroenterology, The Qingzhou Huanglou Hospital, Qingzhou, China
Xinting Pan, Department of Intensive Care Unit, The Affiliated Hospital of Qingdao University, Qingdao, China
Chonggao Yin, Department of Intensive Care Unit, The Affiliated Hospital of Qingdao University, Qingdao, China
Shijie Liu, Department of Intensive Care Unit, The Affiliated Hospital of Qingdao University, Qingdao, China
Fuguo Liu, Department of Intensive Care Unit, The Affiliated Hospital of Qingdao University, Qingdao, China
Ying Sun, Department of Intensive Care Unit, The Affiliated Hospital of Qingdao University, Qingdao, China
Huimin Wang, Department of Intensive Care Unit, The Affiliated Hospital of Qingdao University, Qingdao, China
Yajun Jing, Department of Intensive Care Unit, The Affiliated Hospital of Qingdao University, Qingdao, China
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Abstract
Objective to investigate the expression of NF-κB and occludin in intestinal mucosal barrier during severe acute pancreatitis (SAP) and its clinical significance. Method Twenty-four Wistar rats were randomly divided into a normal control group, an SAP group, and a PDTC group. The rats were sacrificed at 24h after modeling, and general appearance of the abdominal cavity, pathological changes of the intestinal mucosa, changes in intestinal mucosal permeability were observed, serum inflammatory factor IL-1 was measured, and expression of tight junction protein occludin in the intestinal epithelial cells were determined by immunohistochemistry. Results Rats of the SAP group showed obvious inflammatory reaction in the abdominal cavity, manifested by enlargement of intestinal tract, intestinal edema, and increased permeability of intestinal barrier, whereas NF-κB inhibitor alleviated intestinal damage and intestinal mucosa edema, upregulated intestinal epithelial tight junction protein occludin, decreased intestinal permeability, and lowered the level of inflammatory factors. Conclusion Impairment of intestinal barrier function during SAP may be associated with increased NF-κB and decreased intestinal tight junction protein occludin; inhibiting NF-kB may alleviate intestinal mucosal barrier dysfunction during SAP.
Keywords
Severe Acute Pancreatitis, Intestinal Mucosal Barrier, Nf-Κb, Inflammatory Factor
To cite this article
Qingyun Zhu, Shigang Yang, Xinting Pan, Chonggao Yin, Shijie Liu, Fuguo Liu, Ying Sun, Huimin Wang, Yajun Jing, Expression of NF-κB and Occludin in Intestinal Mucosal Barrier During Severe Acute Pancreatitis and Its Clinical Significance, American Journal of Internal Medicine. Vol. 5, No. 5, 2017, pp. 70-73. doi: 10.11648/j.ajim.20170505.11
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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