Expression of NF-κB and Occludin in Intestinal Mucosal Barrier During Severe Acute Pancreatitis and Its Clinical Significance

Qingyun Zhu; Shigang Yang; Xinting Pan; Chonggao Yin; Shijie Liu; Fuguo Liu; Ying Sun; Huimin Wang; Yajun Jing

doi:doi:10.11648/j.ajim.20170505.11

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Expression of NF-κB and Occludin in Intestinal Mucosal Barrier During Severe Acute Pancreatitis and Its Clinical Significance

Qingyun Zhu, Shigang Yang, Xinting Pan, Chonggao Yin, Shijie Liu, Fuguo Liu, Ying Sun, Huimin Wang, Yajun Jing

Published in American Journal of Internal Medicine (Volume 5, Issue 5)

Received: 5 July 2017 Accepted: 14 July 2017 Published: 7 August 2017

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Abstract

Objective to investigate the expression of NF-κB and occludin in intestinal mucosal barrier during severe acute pancreatitis (SAP) and its clinical significance. Method Twenty-four Wistar rats were randomly divided into a normal control group, an SAP group, and a PDTC group. The rats were sacrificed at 24h after modeling, and general appearance of the abdominal cavity, pathological changes of the intestinal mucosa, changes in intestinal mucosal permeability were observed, serum inflammatory factor IL-1 was measured, and expression of tight junction protein occludin in the intestinal epithelial cells were determined by immunohistochemistry. Results Rats of the SAP group showed obvious inflammatory reaction in the abdominal cavity, manifested by enlargement of intestinal tract, intestinal edema, and increased permeability of intestinal barrier, whereas NF-κB inhibitor alleviated intestinal damage and intestinal mucosa edema, upregulated intestinal epithelial tight junction protein occludin, decreased intestinal permeability, and lowered the level of inflammatory factors. Conclusion Impairment of intestinal barrier function during SAP may be associated with increased NF-κB and decreased intestinal tight junction protein occludin; inhibiting NF-kB may alleviate intestinal mucosal barrier dysfunction during SAP.

Published in	American Journal of Internal Medicine (Volume 5, Issue 5)
DOI	10.11648/j.ajim.20170505.11
Page(s)	70-73
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Severe Acute Pancreatitis, Intestinal Mucosal Barrier, Nf-Κb, Inflammatory Factor

References

[1]	Jeon TJ, Park JY. Clinical significance of the neutrophil-lymphocyte ratio as an early predictive marker for adverse outcomes in patients with acute pancreatitis. World J Gastroenterol, 2017, 7; 23 (21): 3883-3889.
[2]	Huang W, Windsor JA. Fulminant or Early Severe Acute Pancreatitis Is Over-looked by Classifications of Severity. Crit Care Med, 2017, 45 (7): e 744-e 745.
[3]	Gomatos IP, Halloran CM, Ghaneh P, et al. Outcomes From Minimal Access Retroperitoneal and Open Pancreatic Necrosectomy in 394 Patients With Necrotizing Pancreatitis. Ann Surg, 2016, 263 (5): 992-1001.
[4]	Deng W, Abliz A, Xu S, et al. Severity of pancreatitis–associated intestinal mucosal barrier injury is reduced following treatment with the NADPH oxidase inhibitor apocynin. Mol Med Rep, 2016, 14 (4): 3525-3534.
[5]	Ouyang J, Zhang ZH, Zhou YX, et al. Up-regulation of Tight-Junction Proteins by p 38 Mitogen-Activated Protein Kinase/p 53 Inhibition Leads to a Reduction of Injury to the Intestinal Mucosal Barrier in Severe Acute Pancreatitis. Pancreas, 2016, 45 (8): 1136-1144.
[6]	Yao P, Cui M, Li Y, et al. Effects of rhubarb on intestinal flora and toll-like receptors of intestinal mucosa in rats with severeacute pancreatitis. Pancreas, 2015, 44 (5): 799-804.
[7]	Xu GF, Guo M, Tian ZQ, et al. Increased of serum high-mobility group box chromosomal protein 1 correlated with intestinalmucosal barrier injury in patients with severe acute pancreatitis. World J Emerg Surg, 2014, 17 (9): 61.
[8]	Zhang XP, Jiang J, Yu YP, et al. Effect of Danshen on apoptosis and NF-κB protein expression of the intestinal mucosa of rats with severe acute pancreatitis or obstructive jaundice. Hepatobiliary Pancreat Dis Int, 2010, 9 (5): 537-546.
[9]	Wang L, Cui YL, Zhang Z, et al. Rhubarb Monomers Protect Intestinal Mucosal Barrier in Sepsis via Junction Proteins. Chin Med J (Engl), 2017, 130 (10): 1218 -1225.
[10]	Kong W, Huang C, Tang Y, et al. Effect of Bacillus subtilis on Aeromonas hydrophila-induced intestinal mucosal barrier function damage and inflammation in grass carp (Ctenopharyngodon idella). Sci Rep, 2017, 7 (1): 1588.
[11]	Watari A, Sakamoto Y, Hisaie K, et al. Rebeccamycin Attenuates TNF-α- Induced Intestinal Epithelial Barrier Dysfunction by Inhibiting Myosin Light Chain Kinase Production. Cell Physiol Biochem, 2017; 41 (5): 1924-1934.
[12]	Li W, Sun K, Ji Y, et al. Glycine Regulates Expression and Distribution of Claudin-7 and ZO-3 Proteins in Intestinal Porcine Epithelial Cells. J Nutr, 2016, 146 (5): 964-969.
[13]	Yuan Y, Han Q, Li S, et al. Wnt 2 b attenuates HSCs activation and liver fibrosis through negative regulating TLR 4 signaling. Sci Rep, 2017, 7 (1): 3952.
[14]	Irene Guijarro-Muñoz, Marta Compte, Ana Álvarez-Cienfuegos, et al. Lipopolysaccharide Activates Toll-like Receptor 4 (TLR 4)-mediated NF-κB Signaling Pathway and Proinflammatory Response in Human Pericytes J Biol Chem, 2014, 289 (4): 2457–2468.
[15]	Serrero M, Planès R, Bahraoui E. PKC-δ isoform plays a crucial role in Tat-TLR 4 signalling pathway to activate NF-κB and CXCL 8 production. Sci Rep, 2017, 7 (1): 2384.
[16]	Nyati KK, Masuda K, Zaman MM, et al. TLR 4-induced NF-κB and MAPK signaling regulate the IL-6 mRNA stabilizing protein Arid 5a. Nucleic Acids Res, 2017, 45 (5): 2687-2703.

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APA Style

Qingyun Zhu, Shigang Yang, Xinting Pan, Chonggao Yin, Shijie Liu, et al. (2017). Expression of NF-κB and Occludin in Intestinal Mucosal Barrier During Severe Acute Pancreatitis and Its Clinical Significance. American Journal of Internal Medicine, 5(5), 70-73. https://doi.org/10.11648/j.ajim.20170505.11

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ACS Style

Qingyun Zhu; Shigang Yang; Xinting Pan; Chonggao Yin; Shijie Liu, et al. Expression of NF-κB and Occludin in Intestinal Mucosal Barrier During Severe Acute Pancreatitis and Its Clinical Significance. Am. J. Intern. Med. 2017, 5(5), 70-73. doi: 10.11648/j.ajim.20170505.11

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AMA Style

Qingyun Zhu, Shigang Yang, Xinting Pan, Chonggao Yin, Shijie Liu, et al. Expression of NF-κB and Occludin in Intestinal Mucosal Barrier During Severe Acute Pancreatitis and Its Clinical Significance. Am J Intern Med. 2017;5(5):70-73. doi: 10.11648/j.ajim.20170505.11

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@article{10.11648/j.ajim.20170505.11,
  author = {Qingyun Zhu and Shigang Yang and Xinting Pan and Chonggao Yin and Shijie Liu and Fuguo Liu and Ying Sun and Huimin Wang and Yajun Jing},
  title = {Expression of NF-κB and Occludin in Intestinal Mucosal Barrier During Severe Acute Pancreatitis and Its Clinical Significance},
  journal = {American Journal of Internal Medicine},
  volume = {5},
  number = {5},
  pages = {70-73},
  doi = {10.11648/j.ajim.20170505.11},
  url = {https://doi.org/10.11648/j.ajim.20170505.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajim.20170505.11},
  abstract = {Objective to investigate the expression of NF-κB and occludin in intestinal mucosal barrier during severe acute pancreatitis (SAP) and its clinical significance. Method Twenty-four Wistar rats were randomly divided into a normal control group, an SAP group, and a PDTC group. The rats were sacrificed at 24h after modeling, and general appearance of the abdominal cavity, pathological changes of the intestinal mucosa, changes in intestinal mucosal permeability were observed, serum inflammatory factor IL-1 was measured, and expression of tight junction protein occludin in the intestinal epithelial cells were determined by immunohistochemistry. Results Rats of the SAP group showed obvious inflammatory reaction in the abdominal cavity, manifested by enlargement of intestinal tract, intestinal edema, and increased permeability of intestinal barrier, whereas NF-κB inhibitor alleviated intestinal damage and intestinal mucosa edema, upregulated intestinal epithelial tight junction protein occludin, decreased intestinal permeability, and lowered the level of inflammatory factors. Conclusion Impairment of intestinal barrier function during SAP may be associated with increased NF-κB and decreased intestinal tight junction protein occludin; inhibiting NF-kB may alleviate intestinal mucosal barrier dysfunction during SAP.},
 year = {2017}
}

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TY  - JOUR
T1  - Expression of NF-κB and Occludin in Intestinal Mucosal Barrier During Severe Acute Pancreatitis and Its Clinical Significance
AU  - Qingyun Zhu
AU  - Shigang Yang
AU  - Xinting Pan
AU  - Chonggao Yin
AU  - Shijie Liu
AU  - Fuguo Liu
AU  - Ying Sun
AU  - Huimin Wang
AU  - Yajun Jing
Y1  - 2017/08/07
PY  - 2017
N1  - https://doi.org/10.11648/j.ajim.20170505.11
DO  - 10.11648/j.ajim.20170505.11
T2  - American Journal of Internal Medicine
JF  - American Journal of Internal Medicine
JO  - American Journal of Internal Medicine
SP  - 70
EP  - 73
PB  - Science Publishing Group
SN  - 2330-4324
UR  - https://doi.org/10.11648/j.ajim.20170505.11
AB  - Objective to investigate the expression of NF-κB and occludin in intestinal mucosal barrier during severe acute pancreatitis (SAP) and its clinical significance. Method Twenty-four Wistar rats were randomly divided into a normal control group, an SAP group, and a PDTC group. The rats were sacrificed at 24h after modeling, and general appearance of the abdominal cavity, pathological changes of the intestinal mucosa, changes in intestinal mucosal permeability were observed, serum inflammatory factor IL-1 was measured, and expression of tight junction protein occludin in the intestinal epithelial cells were determined by immunohistochemistry. Results Rats of the SAP group showed obvious inflammatory reaction in the abdominal cavity, manifested by enlargement of intestinal tract, intestinal edema, and increased permeability of intestinal barrier, whereas NF-κB inhibitor alleviated intestinal damage and intestinal mucosa edema, upregulated intestinal epithelial tight junction protein occludin, decreased intestinal permeability, and lowered the level of inflammatory factors. Conclusion Impairment of intestinal barrier function during SAP may be associated with increased NF-κB and decreased intestinal tight junction protein occludin; inhibiting NF-kB may alleviate intestinal mucosal barrier dysfunction during SAP.
VL  - 5
IS  - 5
ER  -

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Author Information

Qingyun Zhu

Department of Intensive Care Unit, The Affiliated Hospital of Qingdao University, Qingdao, China
Shigang Yang

Department of Gastroenterology, The Qingzhou Huanglou Hospital, Qingzhou, China
Xinting Pan

Department of Intensive Care Unit, The Affiliated Hospital of Qingdao University, Qingdao, China
Chonggao Yin

Department of Intensive Care Unit, The Affiliated Hospital of Qingdao University, Qingdao, China
Shijie Liu

Department of Intensive Care Unit, The Affiliated Hospital of Qingdao University, Qingdao, China
Fuguo Liu

Department of Intensive Care Unit, The Affiliated Hospital of Qingdao University, Qingdao, China
Ying Sun

Department of Intensive Care Unit, The Affiliated Hospital of Qingdao University, Qingdao, China
Huimin Wang

Department of Intensive Care Unit, The Affiliated Hospital of Qingdao University, Qingdao, China
Yajun Jing

Department of Intensive Care Unit, The Affiliated Hospital of Qingdao University, Qingdao, China

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APA Style

Qingyun Zhu, Shigang Yang, Xinting Pan, Chonggao Yin, Shijie Liu, et al. (2017). Expression of NF-κB and Occludin in Intestinal Mucosal Barrier During Severe Acute Pancreatitis and Its Clinical Significance. American Journal of Internal Medicine, 5(5), 70-73. https://doi.org/10.11648/j.ajim.20170505.11

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ACS Style

Qingyun Zhu; Shigang Yang; Xinting Pan; Chonggao Yin; Shijie Liu, et al. Expression of NF-κB and Occludin in Intestinal Mucosal Barrier During Severe Acute Pancreatitis and Its Clinical Significance. Am. J. Intern. Med. 2017, 5(5), 70-73. doi: 10.11648/j.ajim.20170505.11

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AMA Style

Qingyun Zhu, Shigang Yang, Xinting Pan, Chonggao Yin, Shijie Liu, et al. Expression of NF-κB and Occludin in Intestinal Mucosal Barrier During Severe Acute Pancreatitis and Its Clinical Significance. Am J Intern Med. 2017;5(5):70-73. doi: 10.11648/j.ajim.20170505.11

Copy | Download

@article{10.11648/j.ajim.20170505.11,
  author = {Qingyun Zhu and Shigang Yang and Xinting Pan and Chonggao Yin and Shijie Liu and Fuguo Liu and Ying Sun and Huimin Wang and Yajun Jing},
  title = {Expression of NF-κB and Occludin in Intestinal Mucosal Barrier During Severe Acute Pancreatitis and Its Clinical Significance},
  journal = {American Journal of Internal Medicine},
  volume = {5},
  number = {5},
  pages = {70-73},
  doi = {10.11648/j.ajim.20170505.11},
  url = {https://doi.org/10.11648/j.ajim.20170505.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajim.20170505.11},
  abstract = {Objective to investigate the expression of NF-κB and occludin in intestinal mucosal barrier during severe acute pancreatitis (SAP) and its clinical significance. Method Twenty-four Wistar rats were randomly divided into a normal control group, an SAP group, and a PDTC group. The rats were sacrificed at 24h after modeling, and general appearance of the abdominal cavity, pathological changes of the intestinal mucosa, changes in intestinal mucosal permeability were observed, serum inflammatory factor IL-1 was measured, and expression of tight junction protein occludin in the intestinal epithelial cells were determined by immunohistochemistry. Results Rats of the SAP group showed obvious inflammatory reaction in the abdominal cavity, manifested by enlargement of intestinal tract, intestinal edema, and increased permeability of intestinal barrier, whereas NF-κB inhibitor alleviated intestinal damage and intestinal mucosa edema, upregulated intestinal epithelial tight junction protein occludin, decreased intestinal permeability, and lowered the level of inflammatory factors. Conclusion Impairment of intestinal barrier function during SAP may be associated with increased NF-κB and decreased intestinal tight junction protein occludin; inhibiting NF-kB may alleviate intestinal mucosal barrier dysfunction during SAP.},
 year = {2017}
}

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TY  - JOUR
T1  - Expression of NF-κB and Occludin in Intestinal Mucosal Barrier During Severe Acute Pancreatitis and Its Clinical Significance
AU  - Qingyun Zhu
AU  - Shigang Yang
AU  - Xinting Pan
AU  - Chonggao Yin
AU  - Shijie Liu
AU  - Fuguo Liu
AU  - Ying Sun
AU  - Huimin Wang
AU  - Yajun Jing
Y1  - 2017/08/07
PY  - 2017
N1  - https://doi.org/10.11648/j.ajim.20170505.11
DO  - 10.11648/j.ajim.20170505.11
T2  - American Journal of Internal Medicine
JF  - American Journal of Internal Medicine
JO  - American Journal of Internal Medicine
SP  - 70
EP  - 73
PB  - Science Publishing Group
SN  - 2330-4324
UR  - https://doi.org/10.11648/j.ajim.20170505.11
AB  - Objective to investigate the expression of NF-κB and occludin in intestinal mucosal barrier during severe acute pancreatitis (SAP) and its clinical significance. Method Twenty-four Wistar rats were randomly divided into a normal control group, an SAP group, and a PDTC group. The rats were sacrificed at 24h after modeling, and general appearance of the abdominal cavity, pathological changes of the intestinal mucosa, changes in intestinal mucosal permeability were observed, serum inflammatory factor IL-1 was measured, and expression of tight junction protein occludin in the intestinal epithelial cells were determined by immunohistochemistry. Results Rats of the SAP group showed obvious inflammatory reaction in the abdominal cavity, manifested by enlargement of intestinal tract, intestinal edema, and increased permeability of intestinal barrier, whereas NF-κB inhibitor alleviated intestinal damage and intestinal mucosa edema, upregulated intestinal epithelial tight junction protein occludin, decreased intestinal permeability, and lowered the level of inflammatory factors. Conclusion Impairment of intestinal barrier function during SAP may be associated with increased NF-κB and decreased intestinal tight junction protein occludin; inhibiting NF-kB may alleviate intestinal mucosal barrier dysfunction during SAP.
VL  - 5
IS  - 5
ER  -

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