Journal of Surgery

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Helicobacter Pylori Infection in Patients with Chronic Calcular Cholecystitis a Cross- Sectional Study

Received: 09 July 2014    Accepted: 17 July 2014    Published: 20 August 2014
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Abstract

Aims: Helicobacter pylori was found in the bile and gallbladder, so we are aiming to examine the relationship between H. pylori-like bacteria and chronic calcular cholecystitis. METHODS: This cross-sectional study was conducted at our hospitals from May 2011 to January 2012. One hundred and forty patients with chronic calcular cholecystitis were enrolled in this study. Gastroscopy with gastric or duodenal H.pylori urease test and detection of H. pylori in serum were done before the surgical procedure for all patients. After cholecystectomy, the gallbladder tissue was investigated for H. pylori by rapid urease test and Geimsa stain. RESULTS: Of all 140 patients 62.1 % were females, mean age of our patients was 39.04 ± 7.3 years. H. pylori IgG antibody titre was positive in 32 patients with biliary duodenitis, in 43 patients with biliary gastritis, and in 18 patients with duodenal ulcer. The gastric rapid urease test was positive in 40.7 %. The gallbladder rapid urease test was positive in 25 %. In 24 patients both gastric and gallbladder mucosa tested positive for rapid urease test. In 28 patients, the gastric urease test was positive while the gallbladder mucosa tested negative. In 13 patients, the gallbladder mucosa tested positive while the gastric mucosa was negative. There was a significant correlation between the rapid urease test in the gallbladder and in the gastric or duodenal mucosa (P = 0.002). Modified Geimsa staining of gallbladder specimens revealed that H. pylori-like bacteria were detected in 63.6 %. There was a significant correlation between histopathological examination of gallbladder mucosa stained with Geimsa stain and the gallbladder rapid urease test (P < 0.001), but not correlated with the gastric urease test (P = 0.210). CONCLUSION: Our study demonstrates that H. pylori is present in the gallbladder mucosa in patients with chronic calcular cholecystitis and might be an etiological cause of chronic inflammation`.

DOI 10.11648/j.js.20140204.12
Published in Journal of Surgery (Volume 2, Issue 4, August 2014)
Page(s) 58-62
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Cholecystitis, Helicobacter Pylori, Urease Test

References
[1] Arnaout AH, Abbas SH, and Shousha S. H. pylori is not identified in areas of gastric metaplasia of gall bladder. J. Pathol., 160: 333-334, 1990.
[2] Monstein HJ, Jonsson Y, Zdolsek J, Svanvik J. Identification of Helicobacter pylori DNA in human cholesterol gallstones. Scand j. Gastroenterol 2002; 37: 112-119.
[3] Farinato F, Cardin R, Russo VM, Busatto G, Franco M, Rugge M. H. pylori Cag A status, mucosal oxidative damage and gastritis phenotype: a potential pathway to cancer? Helicobacter 2003; 8: 227-234.
[4] Apostolov E, Alsoud WA, Nilson I, Kornilovska I, Usenko V, Lyzogubov V. Helicobacter pylori and other Helicobacter species in gallbladder and liver of patients with chronic cholecystitis detected by immunological and molecular methods. Scan J. Gastroenterol 2005; 40(1): 96-102.
[5] Bohr UR, Kuestewr D, Meyer F. Low prevalence of Helicobacteraceae in gallstone disease and gallbladder carcinoma in the German population. Clin. Microbiol. Infect 13: 525-531, 2007.
[6] Chen DF, Hu L, Yi P, Liu WW, Fang DC, Cao H. Relationship between Helicobacter pylori and chronic cholecystitis. World Chin J. Digestol 2004; 12(8): 1840-1843.
[7] Goodman KJ, Cockburn M. The role of epidemiology in understanding the health effects of Helicobacter pylori. Epidemiology 2001; 12: 266-271.
[8] Devrajani BR, Shah SZA, Soomro AA, Devrajani T. Type 2 diabetes mellitus: A risk factor for Helicobacter pylori infection: A hospital based case-control study. Int J. Diabetes Dev. Ctries 2010; 30: 22-26.
[9] Huffman JL, Schenker S. Acute acalculous cholecystitis- a review. Clin. Gastroenterol Hepatol. 2010; 8(1): 15-22.
[10] Ballal M, Jyothi KN, Antony B, Arun C, Prabhut T, Shivanada PG. Bacteriological spectrum of cholecystitis and its antibiogram. Indian J. Med Microbiol 2001; 19: 212-214.
[11] Silva CP, Pereira-lima JC, Oliveira AG, Guerra JB, Marques DL, Sarmanho L. Association of the presence of Helicobacter in gallbladder tissue with cholelithiasis and cholecystitis. J. Clen Microbiol 2003; 41: 5615-8.
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[13] Randi G, Franceschi S, Vecchina C. Gallbladder cancer worldwide geographical distribution and risk factors. Int J. Cancer 2006; 118: 1591-1602.
[14] Pradhan SB, Dali S. Relation between gallbladder neoplasm and Helicobacter hepaticus infection. Kathmandu Univ. Med J (KUM) 2004; 2: 331-335.
[15] Jiano JZ, Nie QH, Zhao CL, Wu YS, Wen SX, Wu Q. Clinical relationship between Helicobacter pylori infection and chronic hepatopathy. Shijie Huaren Xiaohua Zazhi 2003; 11: 851-853.
[16] Chen W, Li D, Cannon RJ, Stubbs RS. Common presence of Helicobacter DNA in the gallbladder of patients with gallstone diseases and controls. Dig Liver Dis 2003; 35: 237-243.
[17] Chen DF, Hu L, Yi P, Liu WW, Fang DC, Cao H. H.pylori are associated with chronic cholecystitis. World J. Gastroenterol 2007; 13(7): 1119-1122.
[18] Maurer KJ, Carey MC, Fox JG. Role of infection, inflammation, and the immune system in cholesterol gallstone formation. Gastroenterology 2009; 136: 425-40.
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[20] Manoj P. Helicobacter species are associated with possible increase in risk of biliary lithiasis and benign biliary diseases. World J. Surg Oncol 2007; 5: 94-96.
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Author Information
  • Department of Surgery, Faculty of Medicine, Mansoura University ,Mansoura, Egypt

  • Department of Internal Medicine, Faculty of Medicine, Al-Azhar University ,Cairo, Egypt

  • Department of Clinical Pathology, Faculty of Medicine, Mansoura University ,Cairo, Egypt

  • Department of Internal Medicine, Faculty of Medicine, Al-Azhar University ,Cairo, Egypt

  • Department of Internal Medicine, Faculty of Medicine, Al-Azhar University ,Cairo, Egypt

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    Amir A. Fikry, Arafat A. Kassem, Doaa Shahin, Hosam Aldeen Salah Shabana, Sadek Mostafa. (2014). Helicobacter Pylori Infection in Patients with Chronic Calcular Cholecystitis a Cross- Sectional Study. Journal of Surgery, 2(4), 58-62. https://doi.org/10.11648/j.js.20140204.12

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    ACS Style

    Amir A. Fikry; Arafat A. Kassem; Doaa Shahin; Hosam Aldeen Salah Shabana; Sadek Mostafa. Helicobacter Pylori Infection in Patients with Chronic Calcular Cholecystitis a Cross- Sectional Study. J. Surg. 2014, 2(4), 58-62. doi: 10.11648/j.js.20140204.12

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    AMA Style

    Amir A. Fikry, Arafat A. Kassem, Doaa Shahin, Hosam Aldeen Salah Shabana, Sadek Mostafa. Helicobacter Pylori Infection in Patients with Chronic Calcular Cholecystitis a Cross- Sectional Study. J Surg. 2014;2(4):58-62. doi: 10.11648/j.js.20140204.12

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  • @article{10.11648/j.js.20140204.12,
      author = {Amir A. Fikry and Arafat A. Kassem and Doaa Shahin and Hosam Aldeen Salah Shabana and Sadek Mostafa},
      title = {Helicobacter Pylori Infection in Patients with Chronic Calcular Cholecystitis a Cross- Sectional Study},
      journal = {Journal of Surgery},
      volume = {2},
      number = {4},
      pages = {58-62},
      doi = {10.11648/j.js.20140204.12},
      url = {https://doi.org/10.11648/j.js.20140204.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.js.20140204.12},
      abstract = {Aims: Helicobacter pylori was found in the bile and gallbladder, so we are aiming to examine the relationship between H. pylori-like bacteria and chronic calcular cholecystitis. METHODS: This cross-sectional study was conducted at our hospitals from May 2011 to January 2012. One hundred and forty patients with chronic calcular cholecystitis were enrolled in this study. Gastroscopy with gastric or duodenal H.pylori urease test and detection of H. pylori in serum were done before the surgical procedure for all patients. After cholecystectomy, the gallbladder tissue was investigated for H. pylori by rapid urease test and Geimsa stain. RESULTS: Of all 140 patients 62.1 % were females, mean age of our patients was 39.04 ± 7.3 years. H. pylori IgG antibody titre was positive in 32 patients with biliary duodenitis, in 43 patients with biliary gastritis, and in 18 patients with duodenal ulcer. The gastric rapid urease test was positive in 40.7 %. The gallbladder rapid urease test was positive in 25 %. In 24 patients both gastric and gallbladder mucosa tested positive for rapid urease test. In 28 patients, the gastric urease test was positive while the gallbladder mucosa tested negative. In 13 patients, the gallbladder mucosa tested positive while the gastric mucosa was negative. There was a significant correlation between the rapid urease test in the gallbladder and in the gastric or duodenal mucosa (P = 0.002). Modified Geimsa staining of gallbladder specimens revealed that H. pylori-like bacteria were detected in 63.6 %. There was a significant correlation between histopathological examination of gallbladder mucosa stained with Geimsa stain and the gallbladder rapid urease test (P < 0.001), but not correlated with the gastric urease test (P = 0.210). CONCLUSION: Our study demonstrates that H. pylori is present in the gallbladder mucosa in patients with chronic calcular cholecystitis and might be an etiological cause of chronic inflammation`.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Helicobacter Pylori Infection in Patients with Chronic Calcular Cholecystitis a Cross- Sectional Study
    AU  - Amir A. Fikry
    AU  - Arafat A. Kassem
    AU  - Doaa Shahin
    AU  - Hosam Aldeen Salah Shabana
    AU  - Sadek Mostafa
    Y1  - 2014/08/20
    PY  - 2014
    N1  - https://doi.org/10.11648/j.js.20140204.12
    DO  - 10.11648/j.js.20140204.12
    T2  - Journal of Surgery
    JF  - Journal of Surgery
    JO  - Journal of Surgery
    SP  - 58
    EP  - 62
    PB  - Science Publishing Group
    SN  - 2330-0930
    UR  - https://doi.org/10.11648/j.js.20140204.12
    AB  - Aims: Helicobacter pylori was found in the bile and gallbladder, so we are aiming to examine the relationship between H. pylori-like bacteria and chronic calcular cholecystitis. METHODS: This cross-sectional study was conducted at our hospitals from May 2011 to January 2012. One hundred and forty patients with chronic calcular cholecystitis were enrolled in this study. Gastroscopy with gastric or duodenal H.pylori urease test and detection of H. pylori in serum were done before the surgical procedure for all patients. After cholecystectomy, the gallbladder tissue was investigated for H. pylori by rapid urease test and Geimsa stain. RESULTS: Of all 140 patients 62.1 % were females, mean age of our patients was 39.04 ± 7.3 years. H. pylori IgG antibody titre was positive in 32 patients with biliary duodenitis, in 43 patients with biliary gastritis, and in 18 patients with duodenal ulcer. The gastric rapid urease test was positive in 40.7 %. The gallbladder rapid urease test was positive in 25 %. In 24 patients both gastric and gallbladder mucosa tested positive for rapid urease test. In 28 patients, the gastric urease test was positive while the gallbladder mucosa tested negative. In 13 patients, the gallbladder mucosa tested positive while the gastric mucosa was negative. There was a significant correlation between the rapid urease test in the gallbladder and in the gastric or duodenal mucosa (P = 0.002). Modified Geimsa staining of gallbladder specimens revealed that H. pylori-like bacteria were detected in 63.6 %. There was a significant correlation between histopathological examination of gallbladder mucosa stained with Geimsa stain and the gallbladder rapid urease test (P < 0.001), but not correlated with the gastric urease test (P = 0.210). CONCLUSION: Our study demonstrates that H. pylori is present in the gallbladder mucosa in patients with chronic calcular cholecystitis and might be an etiological cause of chronic inflammation`.
    VL  - 2
    IS  - 4
    ER  - 

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