American Journal of Clinical and Experimental Medicine

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In Vitro and in Vivo Anti Dermatophytes Activity of Lawsonia Inermis L. (Henna) Leaves against Ringworm and Its Etiological Agents

Received: 04 May 2014    Accepted: 17 May 2014    Published: 30 May 2014
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Abstract

The study was carried out to identify the etiological agents causing ringworm, evaluate the in vitro and in vivo antifungal activity of Lawsonia inermis L. In vitro study was carried out using agar dilution method. In a total of 50 clinical samples, 4 different species were identified namely; Microsporum canis, Trichophyton tonsurans, Trichophyton rubrum, and Trichophyton mentagrophytes. However, the results showed that hexane extract exerted a strong antifungal activity against all the identified etiological agents, with a minimum inhibitory concentration of 625µg/ml except Microsporum canis which resisted the minimum concentration but susceptible to the higher concentrations. The in vivo study was carried out using 15 naturally infected goats. First, second and third group of animals were treated with henna paste, aqueous and ethanolic extract respectively, fourth group were treated with clotrimazole as a positive control while negative control (fifth) group were left untreated with neither henna nor clotrimazole. The treatments were compared and the results showed that henna paste had the highest efficacy against all the types of ringworm tested compared to the remaining treatments. Disappearance of lesion and complete repair of the hair was observed at 30 days after treatment. Significantly similar result was observed in the group of animals treated with aqueous extract, ethanol extract, and clotrimazole in all the parameters. Significant different was only observed between groups treated and negative control.

DOI 10.11648/j.ajcem.20140203.13
Published in American Journal of Clinical and Experimental Medicine (Volume 2, Issue 3, May 2014)
Page(s) 51-58
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Lawsonia Inermis, Antifungal, Trichophyton, Microsporum, Clotrimazole

References
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[3] Shahitha S, Sa-ranya M and Poornima K. Isolation and Identification of Dermatophytes from Clinical Samples and Antidermatophytic Activity of Lawsonia inermis (Henna plant) International Journal of Pharma-ceutical and Chemical Sciences 2013; 2(2):1050.
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[5] Padron-Marquez B, Viveros-Valdez E, Oranday-Cardenas A and Carran-za-Rosales P. Antifungal activity of Psidium guajava organic extracts against dermatophytic fungi. Journal of Medicinal Plants Research 2012; 6(41):5435-5438.
[6] Mukhtar MD and Huda M. Prevlence of Tinea capitis in primary school and sensitivity of etiological agents on Pistia stratiotes extracts. Nigerian Journal of Microbilogy 2005; 1(19): 412-415.
[7] Falahati M, Nasim OT And Fereshteh J. Anti Dermatophyte Activities of Eucalyptus camaldulensis in Comparison with Griseofulvin Iranian Journal of Pharmacology & Therapeutics 2005; 5(42):80-83.
[8] Fariba B, Hassan R and Homeyra E. In vitro study of the effects of henna extracts (Lawsonia inermis) on Malassezia species. Jundishapur Journal of Microbiology 2010; 3(3):125-128
[9] Kawo AH and Kwa AM. Phytochemical screening and antibacterial activity of the aqueous extracts and fractions of ethanolic extracts of Lawsonia inermis Leaf. International Research Journal of Microbiology 2011; 2(12):510-516.
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[12] Al-Hamadani AH and Al-Mehna BM. Study the effect of Lawsonia inermis extract on the Trichophyton menta-grophytes in vitro and in vivo Coll. of Vet.Med. University of Medical Sciences. Al-Qadissiya Journl 2009; 1(6);115
[13] Rahmoun, NM, Boucherit-Otmani Z, Boucherit K, Benabdallah, M, Villemin D and Choukchou-Braham B. Antibacterial and antifungal activity of lawsone and novel naphtho-quinone derivatives, Med. Mal. Infection 2012; 42(6):270-275.
[14] Adejmo TO. and Bamidele BS. Control of dermatophyte-causing agents using six medicinal plants. Journal of Medicinal Plants Research 2009; 3(11):906–913.
[15] Nasreen K, Hidayatullah A, Abdul Q, Jawed A and Muhammed S. Isolation and identification of dermatophytes from Sindh, Pakistan. Pakistan Jour-nal of Botany 2006; 38(2):493-495.
[16] Mansour-Djaalab H, Kahlouche-Riachi F, Djerrou Z, Serakta-Delmi, M, Hamimed, S, et al. In vitro evaluation of antifungal effects of Lawsonia inermis, Pistacia lentiscus and Juglans regia. International Journal of Medicinal and Aromatic Plants 2012; 2(2): 263-268.
[17] Ahmadian S and Fakhree MA. Henna (Lawsonia inermis) might be used to prevent mycotic infection. Med Hypotheses 2009; (2)73:629-30.
[18] Valderrama JA, Leiva H, Rodriguez JA, Theoduloz C and Schmeda-Hirshmann G. Studies on quinones Synthesis and cyto-toxic evaluation of polyoxyethylenecontaining 1,4-naphthoquinones, Bioorganic and Medicinal Chemistry 2008; 16(7):3687-3693.
[19] Pathirana C, Jensen PR and Fenical W. Marinone and debromomarinone: Antibiotic sesquiterpenoid naphthoquinones of a new structure class from a marine. Scripps Institution of Oceanography 1992; 33(50):7663-7666.
[20] Al-Ani F. K, Younes F. A., Al-Rawashdeh O. F (2002). Ringworm Infection in Cattle and Horses in Jordan . Journal Acta Veterinaria Brno71: 55–60.
[21] Keilah L. Guy Sedar Singor N., Donatien G., R M, P. and Jules-Roger K. In vitro and in vivo antidermatophytic activity of the dichloromethane-methanol (1:1 v/v) extract from the stem bark of Polyscias fulva Hiern (Araliaceae). Complementary and Alternative Medicine 2013, 19: 13:95.
[22] Wagini NH, Soliman AS, Abbas MS, Hanafy YA , Badawy EM, Phytochemical Analysis of Nigerian and Egyptian Henna (Lawsonia Inermis L.) Leaves using TLC, FTIR and GCMS, Plant. 2014, 2(3), 27-32.
Author Information
  • Department of Biology, Faculty of Natural and Applied Sciences, Umaru Musa Yar’adua University, Katsina, Nigeria

  • Department of Natural Resources, Institute of African Research and Studies, Cairo University, Cairo, Egypt

  • Department of Natural Resources, Institute of African Research and Studies, Cairo University, Cairo, Egypt

  • Medical and Aromatic Plants Unit, Desert Research Center, Cairo, Egypt

  • Medicinal and Ornamental Plant, Faculty of Agriculture, Cairo University, Cairo, Egypt

Cite This Article
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    Nasir Hassan Wagini, Mohamed Said Abbas, Amira Shawky Soliman, Yasser Adel Hanafy, El-Saady Mohamed Badawy. (2014). In Vitro and in Vivo Anti Dermatophytes Activity of Lawsonia Inermis L. (Henna) Leaves against Ringworm and Its Etiological Agents. American Journal of Clinical and Experimental Medicine, 2(3), 51-58. https://doi.org/10.11648/j.ajcem.20140203.13

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    ACS Style

    Nasir Hassan Wagini; Mohamed Said Abbas; Amira Shawky Soliman; Yasser Adel Hanafy; El-Saady Mohamed Badawy. In Vitro and in Vivo Anti Dermatophytes Activity of Lawsonia Inermis L. (Henna) Leaves against Ringworm and Its Etiological Agents. Am. J. Clin. Exp. Med. 2014, 2(3), 51-58. doi: 10.11648/j.ajcem.20140203.13

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    AMA Style

    Nasir Hassan Wagini, Mohamed Said Abbas, Amira Shawky Soliman, Yasser Adel Hanafy, El-Saady Mohamed Badawy. In Vitro and in Vivo Anti Dermatophytes Activity of Lawsonia Inermis L. (Henna) Leaves against Ringworm and Its Etiological Agents. Am J Clin Exp Med. 2014;2(3):51-58. doi: 10.11648/j.ajcem.20140203.13

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  • @article{10.11648/j.ajcem.20140203.13,
      author = {Nasir Hassan Wagini and Mohamed Said Abbas and Amira Shawky Soliman and Yasser Adel Hanafy and El-Saady Mohamed Badawy},
      title = {In Vitro and in Vivo Anti Dermatophytes Activity of Lawsonia Inermis L. (Henna) Leaves against Ringworm and Its Etiological Agents},
      journal = {American Journal of Clinical and Experimental Medicine},
      volume = {2},
      number = {3},
      pages = {51-58},
      doi = {10.11648/j.ajcem.20140203.13},
      url = {https://doi.org/10.11648/j.ajcem.20140203.13},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajcem.20140203.13},
      abstract = {The study was carried out to identify the etiological agents causing ringworm, evaluate the in vitro and in vivo antifungal activity of Lawsonia inermis L. In vitro study was carried out using agar dilution method. In a total of 50 clinical samples, 4 different species were identified namely; Microsporum canis, Trichophyton tonsurans, Trichophyton rubrum, and Trichophyton mentagrophytes. However, the results showed that hexane extract exerted a strong antifungal activity against all the identified etiological agents, with a minimum inhibitory concentration of 625µg/ml except Microsporum canis which resisted the minimum concentration but susceptible to the higher concentrations. The in vivo study was carried out using 15 naturally infected goats. First, second and third  group of animals were treated with henna paste, aqueous and ethanolic extract respectively, fourth group were treated with clotrimazole as a positive control while negative control (fifth) group were left untreated with neither henna nor clotrimazole. The treatments were compared and the results showed that henna paste had the highest efficacy against all the types of ringworm tested compared to the remaining treatments. Disappearance of lesion and complete repair of the hair was observed at 30 days after treatment. Significantly similar result was observed in the group of animals treated with aqueous extract, ethanol extract, and clotrimazole in all the parameters. Significant different was only observed between groups treated and negative control.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - In Vitro and in Vivo Anti Dermatophytes Activity of Lawsonia Inermis L. (Henna) Leaves against Ringworm and Its Etiological Agents
    AU  - Nasir Hassan Wagini
    AU  - Mohamed Said Abbas
    AU  - Amira Shawky Soliman
    AU  - Yasser Adel Hanafy
    AU  - El-Saady Mohamed Badawy
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    DO  - 10.11648/j.ajcem.20140203.13
    T2  - American Journal of Clinical and Experimental Medicine
    JF  - American Journal of Clinical and Experimental Medicine
    JO  - American Journal of Clinical and Experimental Medicine
    SP  - 51
    EP  - 58
    PB  - Science Publishing Group
    SN  - 2330-8133
    UR  - https://doi.org/10.11648/j.ajcem.20140203.13
    AB  - The study was carried out to identify the etiological agents causing ringworm, evaluate the in vitro and in vivo antifungal activity of Lawsonia inermis L. In vitro study was carried out using agar dilution method. In a total of 50 clinical samples, 4 different species were identified namely; Microsporum canis, Trichophyton tonsurans, Trichophyton rubrum, and Trichophyton mentagrophytes. However, the results showed that hexane extract exerted a strong antifungal activity against all the identified etiological agents, with a minimum inhibitory concentration of 625µg/ml except Microsporum canis which resisted the minimum concentration but susceptible to the higher concentrations. The in vivo study was carried out using 15 naturally infected goats. First, second and third  group of animals were treated with henna paste, aqueous and ethanolic extract respectively, fourth group were treated with clotrimazole as a positive control while negative control (fifth) group were left untreated with neither henna nor clotrimazole. The treatments were compared and the results showed that henna paste had the highest efficacy against all the types of ringworm tested compared to the remaining treatments. Disappearance of lesion and complete repair of the hair was observed at 30 days after treatment. Significantly similar result was observed in the group of animals treated with aqueous extract, ethanol extract, and clotrimazole in all the parameters. Significant different was only observed between groups treated and negative control.
    VL  - 2
    IS  - 3
    ER  - 

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