American Journal of Clinical and Experimental Medicine

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Biochemical Differentiation and Molecular Characterization of Biofield Treated Vibrio parahaemolyticus

Received: 08 October 2015    Accepted: 19 October 2015    Published: 14 November 2015
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Abstract

The recent emergence of the Vibrio parahaemolyticus (V. parahaemolyticus) is a pandemic. For the safety concern of seafood, consumer monitoring of this organism in seafood is very much essential. The current study was undertaken to evaluate the impact of Mr. Trivedi’s biofield energy treatment on [ATCC-17802] strain of V. parahaemolyticus for its biochemical characteristics, biotype and 16S rDNA analysis. The lyophilized strain of V. parahaemolyticus was divided into two parts, Group (Gr.) I: control and Gr. II: treated. Gr. II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, whereas, Gr. IIB was stored and analyzed on day 142 (Study I). After retreatment of Gr. IIB on day 142 (Study II), the sample was divided into three separate tubes. The tubes first, second and third were analyzed on day 5, 10, and 15, respectively. The biochemical reaction and biotyping were performed using automated MicroScan Walk-Away® system. The 16S rDNA sequencing was carried out to correlate the phylogenetic relationship of V. parahaemolyticus with other bacterial species after the treatment. The results of biochemical reactions were altered 24.24%, out of thirty-three in the treated groups with respect to the control. Moreover, negative (-) reaction of urea was changed to positive (+) in the revived treated Gr. IIB, Study II on day 15 as compared to the control. Besides, biotype number was substantially changed in all the treated groups as compared to the control. However, change in organisms were reported in Gr. IIA on day 10 and in Gr. IIB; Study II on day 5 as Shewanella putrefaciens and Moraxella/Psychrobacter spp., respectively with respect to the control i.e. Vibrio sp. SF. 16S rDNA analysis showed that the identified sample in this experiment was V. parahaemolyticus after biofield treatment, and the nearest homolog genus-species was observed as Vibrio natriegens with 98% gene identity. The results envisaged that the biofield energy treatment showed an alteration in biochemical reaction pattern and biotype number on the strain of V. parahaemolyticus.

DOI 10.11648/j.ajcem.20150305.21
Published in American Journal of Clinical and Experimental Medicine (Volume 3, Issue 5, September 2015)
Page(s) 260-267
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Vibrio parahaemolyticus, Biofield Energy Treatment, Biochemical Reaction, Biotype, 16S rDNA Analysis

References
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Author Information
  • Trivedi Global Inc., Henderson, USA

  • Trivedi Global Inc., Henderson, USA

  • Trivedi Global Inc., Henderson, USA

  • Trivedi Global Inc., Henderson, USA

  • Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India

  • Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India

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    Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, et al. (2015). Biochemical Differentiation and Molecular Characterization of Biofield Treated Vibrio parahaemolyticus. American Journal of Clinical and Experimental Medicine, 3(5), 260-267. https://doi.org/10.11648/j.ajcem.20150305.21

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    Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak; Sambhu Charan Mondal, et al. Biochemical Differentiation and Molecular Characterization of Biofield Treated Vibrio parahaemolyticus. Am. J. Clin. Exp. Med. 2015, 3(5), 260-267. doi: 10.11648/j.ajcem.20150305.21

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    AMA Style

    Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, et al. Biochemical Differentiation and Molecular Characterization of Biofield Treated Vibrio parahaemolyticus. Am J Clin Exp Med. 2015;3(5):260-267. doi: 10.11648/j.ajcem.20150305.21

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  • @article{10.11648/j.ajcem.20150305.21,
      author = {Mahendra Kumar Trivedi and Alice Branton and Dahryn Trivedi and Gopal Nayak and Sambhu Charan Mondal and Snehasis Jana},
      title = {Biochemical Differentiation and Molecular Characterization of Biofield Treated Vibrio parahaemolyticus},
      journal = {American Journal of Clinical and Experimental Medicine},
      volume = {3},
      number = {5},
      pages = {260-267},
      doi = {10.11648/j.ajcem.20150305.21},
      url = {https://doi.org/10.11648/j.ajcem.20150305.21},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajcem.20150305.21},
      abstract = {The recent emergence of the Vibrio parahaemolyticus (V. parahaemolyticus) is a pandemic. For the safety concern of seafood, consumer monitoring of this organism in seafood is very much essential. The current study was undertaken to evaluate the impact of Mr. Trivedi’s biofield energy treatment on [ATCC-17802] strain of V. parahaemolyticus for its biochemical characteristics, biotype and 16S rDNA analysis. The lyophilized strain of V. parahaemolyticus was divided into two parts, Group (Gr.) I: control and Gr. II: treated. Gr. II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, whereas, Gr. IIB was stored and analyzed on day 142 (Study I). After retreatment of Gr. IIB on day 142 (Study II), the sample was divided into three separate tubes. The tubes first, second and third were analyzed on day 5, 10, and 15, respectively. The biochemical reaction and biotyping were performed using automated MicroScan Walk-Away® system. The 16S rDNA sequencing was carried out to correlate the phylogenetic relationship of V. parahaemolyticus with other bacterial species after the treatment. The results of biochemical reactions were altered 24.24%, out of thirty-three in the treated groups with respect to the control. Moreover, negative (-) reaction of urea was changed to positive (+) in the revived treated Gr. IIB, Study II on day 15 as compared to the control. Besides, biotype number was substantially changed in all the treated groups as compared to the control. However, change in organisms were reported in Gr. IIA on day 10 and in Gr. IIB; Study II on day 5 as Shewanella putrefaciens and Moraxella/Psychrobacter spp., respectively with respect to the control i.e. Vibrio sp. SF. 16S rDNA analysis showed that the identified sample in this experiment was V. parahaemolyticus after biofield treatment, and the nearest homolog genus-species was observed as Vibrio natriegens with 98% gene identity. The results envisaged that the biofield energy treatment showed an alteration in biochemical reaction pattern and biotype number on the strain of V. parahaemolyticus.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Biochemical Differentiation and Molecular Characterization of Biofield Treated Vibrio parahaemolyticus
    AU  - Mahendra Kumar Trivedi
    AU  - Alice Branton
    AU  - Dahryn Trivedi
    AU  - Gopal Nayak
    AU  - Sambhu Charan Mondal
    AU  - Snehasis Jana
    Y1  - 2015/11/14
    PY  - 2015
    N1  - https://doi.org/10.11648/j.ajcem.20150305.21
    DO  - 10.11648/j.ajcem.20150305.21
    T2  - American Journal of Clinical and Experimental Medicine
    JF  - American Journal of Clinical and Experimental Medicine
    JO  - American Journal of Clinical and Experimental Medicine
    SP  - 260
    EP  - 267
    PB  - Science Publishing Group
    SN  - 2330-8133
    UR  - https://doi.org/10.11648/j.ajcem.20150305.21
    AB  - The recent emergence of the Vibrio parahaemolyticus (V. parahaemolyticus) is a pandemic. For the safety concern of seafood, consumer monitoring of this organism in seafood is very much essential. The current study was undertaken to evaluate the impact of Mr. Trivedi’s biofield energy treatment on [ATCC-17802] strain of V. parahaemolyticus for its biochemical characteristics, biotype and 16S rDNA analysis. The lyophilized strain of V. parahaemolyticus was divided into two parts, Group (Gr.) I: control and Gr. II: treated. Gr. II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, whereas, Gr. IIB was stored and analyzed on day 142 (Study I). After retreatment of Gr. IIB on day 142 (Study II), the sample was divided into three separate tubes. The tubes first, second and third were analyzed on day 5, 10, and 15, respectively. The biochemical reaction and biotyping were performed using automated MicroScan Walk-Away® system. The 16S rDNA sequencing was carried out to correlate the phylogenetic relationship of V. parahaemolyticus with other bacterial species after the treatment. The results of biochemical reactions were altered 24.24%, out of thirty-three in the treated groups with respect to the control. Moreover, negative (-) reaction of urea was changed to positive (+) in the revived treated Gr. IIB, Study II on day 15 as compared to the control. Besides, biotype number was substantially changed in all the treated groups as compared to the control. However, change in organisms were reported in Gr. IIA on day 10 and in Gr. IIB; Study II on day 5 as Shewanella putrefaciens and Moraxella/Psychrobacter spp., respectively with respect to the control i.e. Vibrio sp. SF. 16S rDNA analysis showed that the identified sample in this experiment was V. parahaemolyticus after biofield treatment, and the nearest homolog genus-species was observed as Vibrio natriegens with 98% gene identity. The results envisaged that the biofield energy treatment showed an alteration in biochemical reaction pattern and biotype number on the strain of V. parahaemolyticus.
    VL  - 3
    IS  - 5
    ER  - 

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