Improved Mechanical Properties of PVA-Chitosan Polymeric Porous Scaffolds for Tissue Engineering
American Journal of Clinical and Experimental Medicine
Volume 3, Issue 5, September 2015, Pages: 268-274
Received: Nov. 12, 2015; Published: Nov. 14, 2015
Views 3519      Downloads 82
Authors
Md. Shariful Islam, Research Institute for Applied Mechanics, Kyushu University, Fukuoka, Japan; Department of Animal Husbandry and Veterinary Science, University of Rajshahi, Rajshahi, Bangladesh
Mitsugu Todo, Research Institute for Applied Mechanics, Kyushu University, Fukuoka, Japan
Article Tools
Follow on us
Abstract
Highly porous HA scaffolds were synthesized from bioceramics by using the polyurethane (PU) sponge template method. The as-prepared HA scaffolds were then fabricated with poly (vinyl alcohol)/chitosan (PVA/CS) and collagen/chitosan (COL/CS) polymeric materials at 4:1 ratio in coating and 2-phase atmospheric condition. Further, the porous microstructure of fabricated biomaterials were characterized by FE-SEM and mechanical properties were tested by using Shimadzu Compact Tabletop Testing Machine EZTest. It was revealed from the study that incorporation of PVA/CS or COL/CS polymeric materials into pure HA scaffolds either coating or 2-phase condition enhanced the mechanical properties of fabricated biomaterials significantly. Biocompatibility of fabricated biomaterials were checked by culturing Human Mesenchymal Stem cell (hMSC) for a period of 7 days over the prepared scaffolds and it was found that hMSC responded well and grown excellently over the all specimens of fabricated scaffolds. Finally, the results revealed that maximum stress value (0.77 MPa) was obtained from HA-PVA/CS 2-phase with cell samples of 7 days culture and followed by HA-PVA/CS coating with cell (0.75 MPa) due to formation of extra cellular matrix (ECM) reinforcement which allowed specimens undergo densification and stress continued to increase.
Keywords
Porous Scaffolds, Mechanical Properties, Biocompatibility, Tissue Engineering, Human Mesenchymal Stem cell (hMSC)
To cite this article
Md. Shariful Islam, Mitsugu Todo, Improved Mechanical Properties of PVA-Chitosan Polymeric Porous Scaffolds for Tissue Engineering, American Journal of Clinical and Experimental Medicine. Vol. 3, No. 5, 2015, pp. 268-274. doi: 10.11648/j.ajcem.20150305.22
References
[1]
E. Wintermantel, J. Mayer, J. Blum, K. L. Eckert, P. Luscher, and M. Mathey, “Tissue engineering scaffolds using superstructures” Biomaterials, 1996, vol.17, pp. 83-91.
[2]
R. C. Thomson, M. C. Wake, M. J. Yaszemski, and A. G. Mikos, “Biodegradable polymer scaffolds to regenerate organs,” Adv. Biopolym., 1997, vol. 122, pp. 245-274.
[3]
A. Persidis, “Tissue engineering” Nat. Biotechnol., 1999, vol.17, pp. 508–510.
[4]
R. F. Service, “Tissue engineers build new bone,” Science, 2000, vo. 289, pp. 1498–1500.
[5]
H. Petite, V. Viateau, W. Bensaid, A. Meunier, C. de Pollak, M. Bourguignon, et al., “Tissue engineered bone regeneration. Nat. Biotechnol., 2000, vol. 18, pp. 959–963.
[6]
M. Jarcho, “Calcium phosphate ceramics as hard tissue prosthetics”, Clin. Orthop. 1981, pp. 259–78.
[7]
L. L. Hench, and J. Wilson, “Surface-active biomaterials,” Science, 1984, vol. 226, pp. 630–706.
[8]
Z. Li, R. H. Ramay, Kip D. Hauch, D. Xiao, and M. Zhang, “Chitosan–alginate hybrid scaffolds for bone tissue engineering,” Biomaterials, 2005, vol. 26, pp. 3919–3928.
[9]
I. L. Hench, “Bioceramics: From concept to clinic,” J. Am. Ceram. Soc., 1991, vol. 74, pp. 1487-1510.
[10]
T. L.T. Kitsugi, T. Yamamuro, T. Nakamura, and M. Oka, “Transmission electron microscopy observations at the interface of bone and four types of calcium phosphate ceramics with different calcium/phosphorus molar ratios” Biomaterials, 1995, vol. 16, pp. 1101-1107.
[11]
R. Murugan and S. Ramakrishna, “Production of ultra-fine bioresorbable carbonated hydroxyapatite,” Acta Biomaterialia, 2006, vol. 2, pp. 201-206.
[12]
B. M. Tracy and R. H. Doremus, “Direct electron microscopy studies of the bone-hydroxylapatite interface,” J. Biomed. Mater. Res., 1984, vol.18, pp. 719-726.
[13]
A.S. Posner and F. Betts, “Synthetic amorphous calcium phosphate and its relation to bone mineral structure,” Accounts Chem. Res., 1975, vol. 8, pp. 273-281.
[14]
Z. L. Raquel. Prog. Cryst. Growth. Ch., 1981, vol. 4, pp. 1-45.
[15]
S. F. Hulbert, F. A. Young, R. S. Mathews, J. J. Klawitter, C. D. Talbert and F. H. Stelling, “Potential of ceramic materials as permanently implantable skeletal prostheses,” J. Biomed. Mater. Res., 1970, vol. 4, pp. 433-456.
[16]
Y. Phanny, “Development and characterization of polymer/bioceramic composite porous biomaterials for bone tissue engineering,” PhD Thesis, Kyushu University, 2014.
[17]
I.V. Yannas, “Regeneration of skin and nerve by use of collagen templates,” In: Nimni, editor. Collagen-III. Boca Raton, FL: CRC Press, 1988. p. 87-115.
[18]
D. Chow, M. L. Nunalee, D. W. Lim, A. J. Simnick, and A. Chilkoti, “Peptide-based biopolymers in biomedicine and biotechnology,” Mater. Sci. Eng. R Rep., 2008, vol. 62, pp. 125-155.
[19]
K. Paipitak, T. Pornpra, P. Mongkontalang, W. Techitdheera, and W. Pecharapa, “Characterization of PVA-Chitosan nanofibers prepared by electrospinning,” Procedia Engineering, 2011, vol. 8, pp.101-105.
[20]
WHO Scientific Group. World Health Organ Tech Rep Ser 919, 2003, 1.
[21]
I. E. Freed and G. Vunjak-novakovic, “Culture of organized cell communities,” Advanced Drug Delivery Reviews, 1998, vol. 33, pp. 15-30.
[22]
M. E. Gomes, A. Salgado, and R. L. Reis, “Bone tissue engineering using starch based scaffolds obtained by different methods,” In: REIS, R. & COHN, D. (eds.) Polymer Based Systems on Tissue Engineering, Replacement and Regeneration, 2002, Springer Netherlands.
[23]
M. S. Islam, Y. Kusumoto, and M. Abdulla-Al-Mamun, “Novel rose-type magnetic (Fe3O4, γ-Fe2O3 and α-Fe2O3) nanoplates synthesized by simple hydrothermal decomposition,” M. Mater. Lett., 2011, vol. 66, pp. 165–167.
[24]
T. Arahira and M. Todo, “Effects of proliferation and differentiation of mesenchymal stem cells on compressive mechanical behavior of collagen/β-tcp composite scaffold,” J. Mechan. Behav. Biomed. Mater., 2014, vol. 39, pp. 218-230.
[25]
L. Sang, D. Luo, S. Xu, X. Wang, and X. Li, “Fabricationand evaluation of biomimetic scaffolds by using collagen-alginate fibrillargels for potential tissue engineering applications,” Mater. Sci. Eng. C, 2011, vol. 31, pp. 262–271.
[26]
E. Landi, A. Tampieri, G. Celotti, and S. Sprio, “Densification behaviour and mechanisms of synthetic hydroxyapatites,” J. Eur. Ceram. Soc., 2000, vol. 20, pp. 2377-2387.
[27]
M. L. Munar, K. I. Udoh, K. Ishikawa, S. Matsuya, and M. Nakagawa, “Effects of sintering temperature over 1,300°C on the physical and compositional properties of porous hydroxyapatite foam,” Dent. Mater. J., 2006, Vol. 25, pp. 51-58.
[28]
N. Shanmugasundaram, P. Ravichandran, P. N. Reddy, N. Ramamurty, S. Pal, and K. P. Rao, “Collagen-chitosan polymeric scaffolds for the in vitro culture of human epidermoid carcinoma cells,” Biomaterials, 2001, vol. 22, pp. 1943-1951.
ADDRESS
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
U.S.A.
Tel: (001)347-983-5186