Evaluation of Immune Biomarkers After Oral Administration of the Novel Herbomineral Formulation Treated with The Trivedi Effect® - Biofield Energy Healing in Male Sprague Dawley Rats
American Journal of Clinical and Experimental Medicine
Volume 5, Issue 6, November 2017, Pages: 209-217
Received: Oct. 30, 2017; Accepted: Nov. 11, 2017; Published: Dec. 11, 2017
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Authors
Mahendra Kumar Trivedi, Trivedi Global, Inc., Henderson, Nevada, USA
Alice Branton, Trivedi Global, Inc., Henderson, Nevada, USA
Dahryn Trivedi, Trivedi Global, Inc., Henderson, Nevada, USA
Gopal Nayak, Trivedi Global, Inc., Henderson, Nevada, USA
Michael Peter Ellis, Trivedi Global, Inc., Henderson, Nevada, USA
James Jeffery Peoples, Trivedi Global, Inc., Henderson, Nevada, USA
James Joseph Meuer, Trivedi Global, Inc., Henderson, Nevada, USA
Johanne Dodon, Trivedi Global, Inc., Henderson, Nevada, USA
John Lawrence Griffin, Trivedi Global, Inc., Henderson, Nevada, USA
John Suzuki, Trivedi Global, Inc., Henderson, Nevada, USA
Joseph Michael Foty, Trivedi Global, Inc., Henderson, Nevada, USA
Judy Weber, Trivedi Global, Inc., Henderson, Nevada, USA
Julia Grace McCammon, Trivedi Global, Inc., Henderson, Nevada, USA
Karen Brynes Allen, Trivedi Global, Inc., Henderson, Nevada, USA
Kathryn Regina Sweas, Trivedi Global, Inc., Henderson, Nevada, USA
Lezley Jo-Anne Wright, Trivedi Global, Inc., Henderson, Nevada, USA
Lisa A. Knoll, Trivedi Global, Inc., Henderson, Nevada, USA
Madeline E. Michaels, Trivedi Global, Inc., Henderson, Nevada, USA
Margaret Kweya Wahl, Trivedi Global, Inc., Henderson, Nevada, USA
Mark E. Stutheit, Trivedi Global, Inc., Henderson, Nevada, USA
Michelle Barnard, Trivedi Global, Inc., Henderson, Nevada, USA
Muriel Mae Ranger, Trivedi Global, Inc., Henderson, Nevada, USA
Paromvong Sinbandhit, Trivedi Global, Inc., Henderson, Nevada, USA
V. J. Kris Elig, Trivedi Global, Inc., Henderson, Nevada, USA
Sambhu Charan Mondal, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
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Abstract
Herbomineral formulations have been used worldwide against various chronic and degenerative diseases due to its fewer side effects. A new proprietary herbomineral formulation was formulated consisted of an ashwagandha root extract and minerals (zinc, magnesium, and selenium). The present study was aimed to evaluate the impact of the Biofield Energy Treated herbomineral formulation in male Sprague Dawley (SD) rats for immune biomarkers modulation. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from twenty renowned Biofield Energy Healers. Biomarkers like immunoglobulins (IgG, IgM), cluster differentiation (CD4+), CD8+, superoxide dismutase (SOD), catalase (CAT), lipid peroxidation (LPO) were monitored. The level of IgM was increased by 4.76% in the Biofield Energy Treated test formulation (G4) compared to the disease control group (G2). The levels of CD4+ and CD8+counts were significantly (p≤0.01) increased by 222.22% and 355.36% in the G4 group compared to the G2 group. The level of lymphocyte was increased by 5% and eosinophil count was significantly decreased by 75% in the G4 group compared to the G2 group. The lipid biomarkers such as total cholesterol (TC), low density lipoprotein (LDL), and very low density lipoprotein (VLDL) were significantly lowered by 9.70%, 6.67%, and 23.54%, respectively in the G4 group compared to the G2 group. The expression of SOD was reduced by 9.96% in the G4 group compared to the G2 group. Further, LPO expression was significantly reduced by 33.38% and 16.88% in the G4 and untreated test formulation (G5) groups, respectively compared to the G2 group. Therefore, it can be concluded that the Biofield Energy Treated test formulation showed significantly improved the cellular and humoral immunity, hematological and biochemical profile compared with the untreated test formulation. As a result, it can be established that The Trivedi Effect®-Biofield Energy Healing has the significant capacity for immunomodulatory effect, which may also be useful in organ transplants, anti-aging, and stress management by improving overall health and quality of life.
Keywords
Biofield Energy Healers, The Trivedi Effect®, Herbomineral Formulation, Hematology, Hepatic Enzymes, Cardiac Biomarker, Anti-aging, Inflammatory Disease and Stress Management
To cite this article
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Michael Peter Ellis, James Jeffery Peoples, James Joseph Meuer, Johanne Dodon, John Lawrence Griffin, John Suzuki, Joseph Michael Foty, Judy Weber, Julia Grace McCammon, Karen Brynes Allen, Kathryn Regina Sweas, Lezley Jo-Anne Wright, Lisa A. Knoll, Madeline E. Michaels, Margaret Kweya Wahl, Mark E. Stutheit, Michelle Barnard, Muriel Mae Ranger, Paromvong Sinbandhit, V. J. Kris Elig, Sambhu Charan Mondal, Snehasis Jana, Evaluation of Immune Biomarkers After Oral Administration of the Novel Herbomineral Formulation Treated with The Trivedi Effect® - Biofield Energy Healing in Male Sprague Dawley Rats, American Journal of Clinical and Experimental Medicine. Vol. 5, No. 6, 2017, pp. 209-217. doi: 10.11648/j.ajcem.20170506.15
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
Goswami TK, Bhar R, Jadhav SE, Joardar SN, Ram GC (2005) Role of dietary zinc as a nutritional immunomodulator. Asian-Australasian Journal of Animal Sciences 18: 439-452.
[2]
Makumire S, Chakravadhanula VS, Köllisch G, Redel E, Shonhai A (2014) Immunomodulatory activity of zinc peroxide (ZnO₂) and titanium dioxide (TiO₂) nanoparticles and their effects on DNA and protein integrity. Toxicol Lett 227: 56-64.
[3]
Sugimoto J, Romani AM, Valentin-Torres AM, Luciano AA, Ramirez Kitchen CM, Funderburg N, Mesiano S, Bernstein HB (2012) Magnesium decreases inflammatory cytokine production: A novel innate immunomodulatory mechanism. J Immunol 188: 6338-6346.
[4]
Salimian J, Arefpour MA, Riazipour M, Poursasan N (2004) Immunomodulatory effects of selenium and vitamin E on alterations in T lymphocyte subsets induced by T-2 toxin. Immunopharmacol Immunotoxicol 36: 275-281.
[5]
Sohat B, Gitter E, Abraham A, Lavie D (1967) Antitumor activity of withaferin A. Can Chemother Rep 51: 51271-51276.
[6]
James SJ, Swenseid M, Makinodan T (1987) Macrophage-mediated depression of T-cell proliferation in zinc-deficient mice. J Nutr 117: 1982-1988.
[7]
Simon R (2011) Genomic biomarkers in predictive medicine: An interim analysis. EMBO Mol Med 3: 429-435.
[8]
Konstantinidis TG, Tsigalou C, Bisiklis A, Romanidou G, Konstantinidou E, Parasidis T, Gioka T, Kampouromiti G, Constantinidis TC, Cassimos DC (2012) Autoantibodies in patients with asthma: Is there a link between asthma and autoimmunity? Int J Immunological Studies 4: 376-387.
[9]
Trakhtenberg EF, Goldberg JL (2011) Immunology. Neuroimmune communication. Science 334: 47-48.
[10]
Berghella AM, Contasta I, Marulli G, D’Innocenzo C, Garofalo F, Gizzi F, Bartolomucci M, Laglia G, Valeri M, Gizzi M, Friscioni M, Barone M, Beato TD, Secinaro E, Pellegrini P (2014) Ageing gender-specific "biomarkers of homeostasis", to protect ourselves against the diseases of the old age. Immun Ageing 11: 3.
[11]
Rubik B (1994) Manual healing methods. Alternative medicine: expanding medical horizons, Washington, DC, US Government Printing Office, NIH Publication No. 94-66.
[12]
Cooper EL (2007) The immune system and complementary and alternative medicine. Evid Based Complement Alternat Med 4: 5-8.
[13]
Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) In vitro evaluation of biofield treatment on viral load against human immunodeficiency-1 and cytomegalo viruses. American Journal of Health Research 3: 338-343.
[14]
Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) In vitro Evaluation of biofield treatment on Enterobacter cloacae: Impact on antimicrobial susceptibility and biotype. J Bacteriol Parasitol 6: 241.
[15]
Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) Evaluation of Biofield Modality on Viral Load of Hepatitis B and C Viruses. J Antivir Antiretrovir 7: 83-88.
[16]
Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) An impact of biofield treatment: Antimycobacterial susceptibility potential using BACTEC 460/MGIT-TB system. Mycobact Dis 5: 189.
[17]
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of antibiogram, genotype and phylogenetic analysis of biofield treated Nocardia otitidis. Biol Syst Open Access 4: 143.
[18]
Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015) Antibiogram, biochemical reactions, and genotypic pattern of biofield treated Pseudomonas aeruginosa. J Trop Dis 4: 181.
[19]
Trivedi MK, Patil S, Tallapragada RM (2013) Effect of bio field treatment on the physical and thermal characteristics of vanadium pentoxide powders. J Material Sci Eng S 11: 001.
[20]
Trivedi MK, Branton A, Trivedi D, Shettigar H, Bairwa K, Jana S (2015) Fourier transform infrared and ultraviolet-visible spectroscopic characterization of biofield treated salicylic acid and sparfloxacin. Nat Prod Chem Res 3: 186.
[21]
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Potential impact of biofield treatment on atomic and physical characteristics of magnesium. Vitam Miner 3: 129.
[22]
Trivedi MK, Branton A, Trivedi D, Nayak G, Sethi KK, Jana S (2016) Gas chromatography-mass spectrometry based isotopic abundance ratio analysis of biofield energy treated methyl-2-napthylether (Nerolin). American Journal of Physical Chemistry 5: 80-86.
[23]
Trivedi MK, Branton A, Trivedi D, Nayak G, Panda P, Jana S (2016) Gas chromatography-mass spectrometric analysis of isotopic abundance of 13C, 2H, and 18O in biofield energy treated p-tertiary butylphenol (PTBP). American Journal of Chemical Engineering 4: 78-86.
[24]
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of biochemical marker - glutathione and DNA fingerprinting of biofield energy treated Oryza sativa. American Journal of BioScience 3: 243-248.
[25]
Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2016) Molecular analysis of biofield treated eggplant and watermelon crops. Adv Crop Sci Tech 4: 208.
[26]
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Physical, atomic and thermal properties of biofield treated lithium powder. J Adv Chem Eng 5: 136.
[27]
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Evaluation of biofield energy treatment on physical and thermal characteristics of selenium powder. Journal of Food and Nutrition Sciences 3: 223-228.
[28]
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Mishra RK, Latiyal O, Jana S (2015) Physicochemical characterization of biofield energy treated calcium carbonate powder. American Journal of Health Research 3: 368-375.
[29]
Ladics GS (2007) Primary immune response to sheep red blood cells (SRBC) as the conventional T-cell dependent antibody response (TDAR) test. J Immunotoxicol 4: 149-152.
[30]
Temple L, Kawabata TT, Munson AE, White KL Jr. (1993) Comparison of ELISA and plaque-forming cell assays for measuring the humoral immune response to SRBC in rats and mice treated with benzo[a]pyrene or cyclophosphamide. Fundam Appl Toxicol 21: 412-419.
[31]
Ahmed SG, Uraka A (2010) Eosinopenia as a marker of infection in patients with sickle cell anaemia: A preliminary report. International Journal of Biomedical and Health Sciences 6: 57-61.
[32]
Ose L, Aass B, Christophersen B (1995) A rapid analysis of the lipid profile. A comparative study of different analytical methods for determination of blood lipids. Tidsskr Nor Laegeforen 115: 3487-3489.
[33]
Ahera V, Wahib AK (2002) Biotechnological approach to evaluate the immunomodulatory activity of ethanolic extract of Tinospora cordifolia stem (mango plant climber). Iran J Pharm Res 11: 863-872.
[34]
Malik F, Singh J, Khajuria A, Suri KA, Satti NK, Singh S, Kaul MK, Kumar A, Bhatia A, Qazi GN (2007) A standardized root extract of Withania somnifera and its major constituent withanolide-A elicit humoral and cell-mediated immune responses by up regulation of Th1-dominant polarization in BALB/c mice. Life Sci 80: 1525-1538.
[35]
Spallholz JE, Stewart JR (1989) Advances in the role of minerals in immunobiology. Biol Trace Elem Res 19: 129-151.
[36]
Pescovitz MD, Lunney JK, Sachs DH (1984) Preparation and characterization of monoclonal antibodies reactive with porcine PBL. J Immunol 133: 368-375.
[37]
Berg RE, Forman J (2006) The role of CD8 T cells in innate immunity and in antigen non-specific protection. Curr Opin Immunol 18: 338-343.
[38]
Jung Y, Rothenberg ME (2014) Roles and regulation of gastrointestinal eosinophils in immunity and disease. J Immunol 193: 999-1005.
[39]
Galioto AM, Hess JA, Nolan TJ, Schad GA, Lee JJ, Abraham D (2006) Role of eosinophils and neutrophils in innate and adaptive protective immunity to larval Strongyloides stercoralis in mice. Infect Immun 74: 5730-5738.
[40]
Marken PA, Weart CW, Carson DS, Gums JG, Lopes-Virella MF (1989) Effects of magnesium oxide on the lipid profile of healthy volunteers. Atherosclerosis 77: 37-42.
[41]
Lal J, Vasudev K, Kela AK, Jain SK (2003) Effect of oral magnesium supplementation on the lipid profile and blood glucose of patients with type 2 diabetes mellitus. J Assoc Physicians India 51: 37-42.
[42]
Hercberg S, Bertrais S, Czernichow S, Noisette N, Galan P, Jaouen A, Tichet J, Briancon S, Favier A, Mennen L, Roussel AM (2005) Alterations of the lipid profile after 7.5 years of low-dose antioxidant supplementation in the SU.VI.MAX Study. Lipids 40: 335-342.
[43]
Grossman CJ (1985) Interactions between the gonadal steroids and the immune system. Science 227: 257-261.
[44]
Hillgarth N, Wingfield JC (1997) Testosterone and immunosuppression in vertebrates: implications for parasite-mediated sexual selection. In: Host-parasite evolution: general principles and avian models (Clayton DH, Moore J, eds). Oxford University Press; 78-104.
[45]
John JL (1994) The avian spleen: A neglected organ. Q Rev Biol 69: 327-351.
[46]
Rifé SU, Márquez MG, Escalante A, Velich T (1990) The effect of testosterone on the immune response. Mechanism of action on antibody-forming cells. Immunol Invest 19: 259-270.
[47]
https://med.stanford.edu/news/all-news/2013/12/in-men-high-testosterone-can-mean-weakened-immune-response-study-finds.html
[48]
Wang C, Yue X, Lu X, Liu B (2013) The role of catalase in the immune response to oxidative stress and pathogen challenge in the clam Meretrix meretrix. Fish Shellfish Immunol 34: 91-99.
[49]
Vigneshkumar B, Pandian SK, Balamurugan K (2013) Catalase activity and innate immune response of Caenorhabditis elegans against the heavy metal toxin lead. Environ Toxicol 28: 313-321.
[50]
Marikovsky M, Ziv V, Nevo N, Harris-Cerruti C, Ori Mahler O (2003) Cu/Zn Superoxide dismutase plays important role in immune response. The Journal of Immunology 170: 2993-3001.
[51]
Rao KM (2001) MAP kinase activation in macrophages. J Leukocyte Biol 69: 3.
[52]
Dhiman M, Coronado YA, Vallejo CK, Petersen JR, Ejilemele A, Nuñez S, Zago MP, Spratt H, Garg NJ (2013) Innate immune responses and antioxidant/oxidant imbalance are major determinants of human Chagas disease. PLoS Negl Trop Dis 7: e2364.
[53]
Lodi S, Sharma V, Kansal L (2011) The protective effect of Rubia cordifolia against lead nitrate-induced immune response impairment and kidney oxidative damage. Indian J Pharmacol 43: 441-444.
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