American Journal of Clinical and Experimental Medicine

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Oral Supplementation of L-Arginine Improves Ventricular Remodeling by Regulating Angiogenesis and Collagen Synthesis in Myocardial Infarction Rats

Received: 08 April 2019    Accepted: 09 May 2019    Published: 09 July 2019
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Abstract

[Objective] To investigate the effect of angiogenesis and collagen synthesis in peri-infarct area of the L-Arginine therapy for acute myocardial infarction rats. [Methods] The acute myocardial infarction rats model was established by ligation of the left anterior descending of coronary artery. Thirty male Sprague-Dawley rats were randomly divided into three groups: L-Arginine group, sham group, normal saline group (NS group). Four weeks after ligation, cardiac function, scar area, plasma concentration of BNP, angiogenesis and arteriogenesis, myocardial collagen I and eNOS protein, the mRNA expression of collagen I were studied. Echocardiography, Masson staining, enzyme-linked immunosorbent assay (ELISA), immunehistochemistry, western blot and quantitative polymerase chain (qPCR) reaction were performed. [Results] Four weeks after ligation, compared with the control group, LVEF, LVFS were higher in L-Arginine group, While LVEDD and LVESD decreased (P < 0.01). Average scar percentage and plasma concentration of BNP were lower in L-Arginine group (P < 0.01). The CD31-positive microvessels and α-SMA positive microvessels in peri-infarct area were higher in L-Arginine group (P < 0.01), while collagen I protein and mRNA expression was decreased in this group (P < 0.01). [Conclusions] L-Arginine improves cardiac function and reduces infarction size in AMI rats, the possible mechanism is related to dual function of promoting angiogenesis and arteriogenesis, regulating collagen I expression is also one of the important mechanisms.

DOI 10.11648/j.ajcem.20190702.12
Published in American Journal of Clinical and Experimental Medicine (Volume 7, Issue 2, March 2019)
Page(s) 47-53
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Acute Myocardial Infarction, Angiogenesis, L-Arginine

References
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Author Information
  • Department of Interventional Medical Center, the Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, People's Republic of China

  • Department of Cardiology, the Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, People's Republic of China

  • Department of Cardiology, the Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, People's Republic of China

  • Department of Cardiology, the Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, People's Republic of China

  • Department of Cardiology, the Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, People's Republic of China

  • Department of Cardiology, the Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, People's Republic of China

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    Yonghua Liu, Zhijuan Zhou, Zhiling Zhu, Wenyi Tang, Liyun Luo, et al. (2019). Oral Supplementation of L-Arginine Improves Ventricular Remodeling by Regulating Angiogenesis and Collagen Synthesis in Myocardial Infarction Rats. American Journal of Clinical and Experimental Medicine, 7(2), 47-53. https://doi.org/10.11648/j.ajcem.20190702.12

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    ACS Style

    Yonghua Liu; Zhijuan Zhou; Zhiling Zhu; Wenyi Tang; Liyun Luo, et al. Oral Supplementation of L-Arginine Improves Ventricular Remodeling by Regulating Angiogenesis and Collagen Synthesis in Myocardial Infarction Rats. Am. J. Clin. Exp. Med. 2019, 7(2), 47-53. doi: 10.11648/j.ajcem.20190702.12

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    AMA Style

    Yonghua Liu, Zhijuan Zhou, Zhiling Zhu, Wenyi Tang, Liyun Luo, et al. Oral Supplementation of L-Arginine Improves Ventricular Remodeling by Regulating Angiogenesis and Collagen Synthesis in Myocardial Infarction Rats. Am J Clin Exp Med. 2019;7(2):47-53. doi: 10.11648/j.ajcem.20190702.12

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  • @article{10.11648/j.ajcem.20190702.12,
      author = {Yonghua Liu and Zhijuan Zhou and Zhiling Zhu and Wenyi Tang and Liyun Luo and Chen Jian},
      title = {Oral Supplementation of L-Arginine Improves Ventricular Remodeling by Regulating Angiogenesis and Collagen Synthesis in Myocardial Infarction Rats},
      journal = {American Journal of Clinical and Experimental Medicine},
      volume = {7},
      number = {2},
      pages = {47-53},
      doi = {10.11648/j.ajcem.20190702.12},
      url = {https://doi.org/10.11648/j.ajcem.20190702.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajcem.20190702.12},
      abstract = {[Objective] To investigate the effect of angiogenesis and collagen synthesis in peri-infarct area of the L-Arginine therapy for acute myocardial infarction rats. [Methods] The acute myocardial infarction rats model was established by ligation of the left anterior descending of coronary artery. Thirty male Sprague-Dawley rats were randomly divided into three groups: L-Arginine group, sham group, normal saline group (NS group). Four weeks after ligation, cardiac function, scar area, plasma concentration of BNP, angiogenesis and arteriogenesis, myocardial collagen I and eNOS protein, the mRNA expression of collagen I were studied. Echocardiography, Masson staining, enzyme-linked immunosorbent assay (ELISA), immunehistochemistry, western blot and quantitative polymerase chain (qPCR) reaction were performed. [Results] Four weeks after ligation, compared with the control group, LVEF, LVFS were higher in L-Arginine group, While LVEDD and LVESD decreased (P < 0.01). Average scar percentage and plasma concentration of BNP were lower in L-Arginine group (P < 0.01). The CD31-positive microvessels and α-SMA positive microvessels in peri-infarct area were higher in L-Arginine group (P < 0.01), while collagen I protein and mRNA expression was decreased in this group (P < 0.01). [Conclusions] L-Arginine improves cardiac function and reduces infarction size in AMI rats, the possible mechanism is related to dual function of promoting angiogenesis and arteriogenesis, regulating collagen I expression is also one of the important mechanisms.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Oral Supplementation of L-Arginine Improves Ventricular Remodeling by Regulating Angiogenesis and Collagen Synthesis in Myocardial Infarction Rats
    AU  - Yonghua Liu
    AU  - Zhijuan Zhou
    AU  - Zhiling Zhu
    AU  - Wenyi Tang
    AU  - Liyun Luo
    AU  - Chen Jian
    Y1  - 2019/07/09
    PY  - 2019
    N1  - https://doi.org/10.11648/j.ajcem.20190702.12
    DO  - 10.11648/j.ajcem.20190702.12
    T2  - American Journal of Clinical and Experimental Medicine
    JF  - American Journal of Clinical and Experimental Medicine
    JO  - American Journal of Clinical and Experimental Medicine
    SP  - 47
    EP  - 53
    PB  - Science Publishing Group
    SN  - 2330-8133
    UR  - https://doi.org/10.11648/j.ajcem.20190702.12
    AB  - [Objective] To investigate the effect of angiogenesis and collagen synthesis in peri-infarct area of the L-Arginine therapy for acute myocardial infarction rats. [Methods] The acute myocardial infarction rats model was established by ligation of the left anterior descending of coronary artery. Thirty male Sprague-Dawley rats were randomly divided into three groups: L-Arginine group, sham group, normal saline group (NS group). Four weeks after ligation, cardiac function, scar area, plasma concentration of BNP, angiogenesis and arteriogenesis, myocardial collagen I and eNOS protein, the mRNA expression of collagen I were studied. Echocardiography, Masson staining, enzyme-linked immunosorbent assay (ELISA), immunehistochemistry, western blot and quantitative polymerase chain (qPCR) reaction were performed. [Results] Four weeks after ligation, compared with the control group, LVEF, LVFS were higher in L-Arginine group, While LVEDD and LVESD decreased (P < 0.01). Average scar percentage and plasma concentration of BNP were lower in L-Arginine group (P < 0.01). The CD31-positive microvessels and α-SMA positive microvessels in peri-infarct area were higher in L-Arginine group (P < 0.01), while collagen I protein and mRNA expression was decreased in this group (P < 0.01). [Conclusions] L-Arginine improves cardiac function and reduces infarction size in AMI rats, the possible mechanism is related to dual function of promoting angiogenesis and arteriogenesis, regulating collagen I expression is also one of the important mechanisms.
    VL  - 7
    IS  - 2
    ER  - 

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