Oral Supplementation of L-Arginine Improves Ventricular Remodeling by Regulating Angiogenesis and Collagen Synthesis in Myocardial Infarction Rats
American Journal of Clinical and Experimental Medicine
Volume 7, Issue 2, March 2019, Pages: 47-53
Received: Apr. 8, 2019; Accepted: May 9, 2019; Published: Jul. 9, 2019
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Authors
Yonghua Liu, Department of Interventional Medical Center, the Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, People's Republic of China
Zhijuan Zhou, Department of Cardiology, the Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, People's Republic of China
Zhiling Zhu, Department of Cardiology, the Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, People's Republic of China
Wenyi Tang, Department of Cardiology, the Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, People's Republic of China
Liyun Luo, Department of Cardiology, the Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, People's Republic of China
Chen Jian, Department of Cardiology, the Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, People's Republic of China
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Abstract
[Objective] To investigate the effect of angiogenesis and collagen synthesis in peri-infarct area of the L-Arginine therapy for acute myocardial infarction rats. [Methods] The acute myocardial infarction rats model was established by ligation of the left anterior descending of coronary artery. Thirty male Sprague-Dawley rats were randomly divided into three groups: L-Arginine group, sham group, normal saline group (NS group). Four weeks after ligation, cardiac function, scar area, plasma concentration of BNP, angiogenesis and arteriogenesis, myocardial collagen I and eNOS protein, the mRNA expression of collagen I were studied. Echocardiography, Masson staining, enzyme-linked immunosorbent assay (ELISA), immunehistochemistry, western blot and quantitative polymerase chain (qPCR) reaction were performed. [Results] Four weeks after ligation, compared with the control group, LVEF, LVFS were higher in L-Arginine group, While LVEDD and LVESD decreased (P < 0.01). Average scar percentage and plasma concentration of BNP were lower in L-Arginine group (P < 0.01). The CD31-positive microvessels and α-SMA positive microvessels in peri-infarct area were higher in L-Arginine group (P < 0.01), while collagen I protein and mRNA expression was decreased in this group (P < 0.01). [Conclusions] L-Arginine improves cardiac function and reduces infarction size in AMI rats, the possible mechanism is related to dual function of promoting angiogenesis and arteriogenesis, regulating collagen I expression is also one of the important mechanisms.
Keywords
Acute Myocardial Infarction, Angiogenesis, L-Arginine
To cite this article
Yonghua Liu, Zhijuan Zhou, Zhiling Zhu, Wenyi Tang, Liyun Luo, Chen Jian, Oral Supplementation of L-Arginine Improves Ventricular Remodeling by Regulating Angiogenesis and Collagen Synthesis in Myocardial Infarction Rats, American Journal of Clinical and Experimental Medicine. Vol. 7, No. 2, 2019, pp. 47-53. doi: 10.11648/j.ajcem.20190702.12
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This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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