American Journal of Clinical and Experimental Medicine

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Methylation of Epstein-Barr Virus-Associated Gastric Cancer Suppressor Genes

Received: 29 May 2019    Accepted:     Published: 29 July 2019
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Abstract

Objective: To identify the methylation silenced tumor suppressor genes in Epstein-Barr virus (EBV)-associated gastric cancer (EBVaGC). Methods: EBV-positive (GT38, PT and SNU719) and negative (SGC7901) gastric cancer cell lines were selected and treated with 5-Aza-CdR. Then real-time fluorescence quantitative PCR was used to validate the results of microarray, and methylation-specific PCR (MSP) and bisulfite genomic sequencing (BGS) were adopted to detect the CpG island methylation levels of gene promoters. Results: The expression levels of 6 differentially expressed genes (H19, LOXL1, ARMCX2, LXN, CDH3 and MMP7) before and after 5-Aza-CdR treatment were confirmed by real-time qPCR, which are consistent with the results of microarray analysis. There were different degrees of methylation in LOXL1 gene promoter in EBVaGC. GT38 and PT were fully methylated, and SGC7901 and HGC-27 was unmethylated, suggesting that this gene is a candidate methylation silenced tumor suppressor gene. The methylation rate of LOXL1 in EBVaGC was significantly higher than that in EBV-negative gastric cancer (EBVnGC). Conclusion: The promoter region of candidate tumor suppressor gene LOXL1 shows high methylation status, indicating that EBV critically accounts for the methylation of LOXL1 gene regulatory region. EBV is involved in the pathogensis of EBVaGC that aberrant methylation occurred in promoter CpG island, which inactivates tumor suppressor genes.

DOI 10.11648/j.ajcem.20190702.13
Published in American Journal of Clinical and Experimental Medicine (Volume 7, Issue 2, March 2019)
Page(s) 54-60
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Epstein-Barr Virus, Gastric Cancer, Methylation, Tumor Suppressor Gene

References
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Author Information
  • Department of Gastroenterology, Binzhou People's Hospital, Binzhou City, P. R. China

  • Department of Neurology, Binzhou People's Hospital, Binzhou City, P. R. China

  • Department of Hepatobiliary Surgery, Binzhou People's Hospital, Binzhou City, P. R. China

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  • APA Style

    Tingting Chen, Donghui Tian, Yurong Zhang. (2019). Methylation of Epstein-Barr Virus-Associated Gastric Cancer Suppressor Genes. American Journal of Clinical and Experimental Medicine, 7(2), 54-60. https://doi.org/10.11648/j.ajcem.20190702.13

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    ACS Style

    Tingting Chen; Donghui Tian; Yurong Zhang. Methylation of Epstein-Barr Virus-Associated Gastric Cancer Suppressor Genes. Am. J. Clin. Exp. Med. 2019, 7(2), 54-60. doi: 10.11648/j.ajcem.20190702.13

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    AMA Style

    Tingting Chen, Donghui Tian, Yurong Zhang. Methylation of Epstein-Barr Virus-Associated Gastric Cancer Suppressor Genes. Am J Clin Exp Med. 2019;7(2):54-60. doi: 10.11648/j.ajcem.20190702.13

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  • @article{10.11648/j.ajcem.20190702.13,
      author = {Tingting Chen and Donghui Tian and Yurong Zhang},
      title = {Methylation of Epstein-Barr Virus-Associated Gastric Cancer Suppressor Genes},
      journal = {American Journal of Clinical and Experimental Medicine},
      volume = {7},
      number = {2},
      pages = {54-60},
      doi = {10.11648/j.ajcem.20190702.13},
      url = {https://doi.org/10.11648/j.ajcem.20190702.13},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajcem.20190702.13},
      abstract = {Objective: To identify the methylation silenced tumor suppressor genes in Epstein-Barr virus (EBV)-associated gastric cancer (EBVaGC). Methods: EBV-positive (GT38, PT and SNU719) and negative (SGC7901) gastric cancer cell lines were selected and treated with 5-Aza-CdR. Then real-time fluorescence quantitative PCR was used to validate the results of microarray, and methylation-specific PCR (MSP) and bisulfite genomic sequencing (BGS) were adopted to detect the CpG island methylation levels of gene promoters. Results: The expression levels of 6 differentially expressed genes (H19, LOXL1, ARMCX2, LXN, CDH3 and MMP7) before and after 5-Aza-CdR treatment were confirmed by real-time qPCR, which are consistent with the results of microarray analysis. There were different degrees of methylation in LOXL1 gene promoter in EBVaGC. GT38 and PT were fully methylated, and SGC7901 and HGC-27 was unmethylated, suggesting that this gene is a candidate methylation silenced tumor suppressor gene. The methylation rate of LOXL1 in EBVaGC was significantly higher than that in EBV-negative gastric cancer (EBVnGC). Conclusion: The promoter region of candidate tumor suppressor gene LOXL1 shows high methylation status, indicating that EBV critically accounts for the methylation of LOXL1 gene regulatory region. EBV is involved in the pathogensis of EBVaGC that aberrant methylation occurred in promoter CpG island, which inactivates tumor suppressor genes.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Methylation of Epstein-Barr Virus-Associated Gastric Cancer Suppressor Genes
    AU  - Tingting Chen
    AU  - Donghui Tian
    AU  - Yurong Zhang
    Y1  - 2019/07/29
    PY  - 2019
    N1  - https://doi.org/10.11648/j.ajcem.20190702.13
    DO  - 10.11648/j.ajcem.20190702.13
    T2  - American Journal of Clinical and Experimental Medicine
    JF  - American Journal of Clinical and Experimental Medicine
    JO  - American Journal of Clinical and Experimental Medicine
    SP  - 54
    EP  - 60
    PB  - Science Publishing Group
    SN  - 2330-8133
    UR  - https://doi.org/10.11648/j.ajcem.20190702.13
    AB  - Objective: To identify the methylation silenced tumor suppressor genes in Epstein-Barr virus (EBV)-associated gastric cancer (EBVaGC). Methods: EBV-positive (GT38, PT and SNU719) and negative (SGC7901) gastric cancer cell lines were selected and treated with 5-Aza-CdR. Then real-time fluorescence quantitative PCR was used to validate the results of microarray, and methylation-specific PCR (MSP) and bisulfite genomic sequencing (BGS) were adopted to detect the CpG island methylation levels of gene promoters. Results: The expression levels of 6 differentially expressed genes (H19, LOXL1, ARMCX2, LXN, CDH3 and MMP7) before and after 5-Aza-CdR treatment were confirmed by real-time qPCR, which are consistent with the results of microarray analysis. There were different degrees of methylation in LOXL1 gene promoter in EBVaGC. GT38 and PT were fully methylated, and SGC7901 and HGC-27 was unmethylated, suggesting that this gene is a candidate methylation silenced tumor suppressor gene. The methylation rate of LOXL1 in EBVaGC was significantly higher than that in EBV-negative gastric cancer (EBVnGC). Conclusion: The promoter region of candidate tumor suppressor gene LOXL1 shows high methylation status, indicating that EBV critically accounts for the methylation of LOXL1 gene regulatory region. EBV is involved in the pathogensis of EBVaGC that aberrant methylation occurred in promoter CpG island, which inactivates tumor suppressor genes.
    VL  - 7
    IS  - 2
    ER  - 

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