Effects of shRNA Expression Vector-mediated Glutathione S-transferase P1 Gene Silencing on Prostate Cancer DU145 Cells
American Journal of Clinical and Experimental Medicine
Volume 7, Issue 5, September 2019, Pages: 119-125
Received: Nov. 18, 2019;
Published: Nov. 18, 2019
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Ruirui Xu, Department of Nephrology, Binzhou People's Hospital, Binzhou City, P. R. China
Zhufu Shao, Department of Critical Care Medicine, Binzhou People's Hospital, Binzhou City, P. R. China
Zhi Xiao, Department of Nephrology, Binzhou People's Hospital, Binzhou City, P. R. China
Objective: This study aims to explore the impact of silent GSTP1 gene on the proliferation activity, cell cycle and apoptotic rate of the androgen-independent prostate cancer cell strain DU145. Methods: Thirty subjects with benign prostatic hyperplasia (BPH) who had been subject to urinary surgical resection and pathological diagnosis, 30 subjects with radically cured prostate cancer (PCa), and another 30 subjects with hormone refractory prostate cancer (HRPC) were enrolled. The GSTP1 expression in the subjects was tested with the immunohistochemical S-P method, the GSTP1 expression in the blood serum of the patients with prostatic hyperplasia and prostate cancer was tested with Elisa, and the mRNA level of the GSTP1 gene after the DUl45 cells were transfected with shRNA was tested. Results: Four of the HRPC patients were complicated with bone metastasis, two with bladder invasion, and five with liver and lung metastasis. GSTP1 showed high expression in tissues with prostatic hyperplasia and hormone refractory prostate cancer and low expression in tissues with prostate cancer, and the differences were statistically significant (P<0.05). Difference was found among the serum concentrations of the BPH group, the PCa group and the HRPC group. The HRPC group showed serum GSTP1 concentration higher than that of the PCa group (P<0.01), the BPH group also showed serum GSTP1 concentration higher than that of the PCa group (P<0.01), and the difference between the serum concentrations of the BPH group and the HRPC group was not statistically significant (P>0.05). The GSTP1 mRNA content decreased significantly after shRNA554 intervention, with an IOD value of 67.1±8.7. The intervention effect was evidently lower than that of the carrier shRNA255 group (162.2±12.6) and the shRNA593 group (114.5±10.6) with statistically significant difference (P<0.01). Conclusion: The expression vector-mediated GSTP1-shRNA554 transfection can reduce the mRNA level of the GSTP1 gene of the androgen-independent prostate cancer DU145 cells and inhibit the proliferation activity of the in vitro cultivated DU145 cells.
Effects of shRNA Expression Vector-mediated Glutathione S-transferase P1 Gene Silencing on Prostate Cancer DU145 Cells, American Journal of Clinical and Experimental Medicine.
Vol. 7, No. 5,
2019, pp. 119-125.
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