Potential Ex-vivo Anti-inflammatory, Cardioprotective Properties and Phytochemical Properties of Leaves of Mussaenda Roxburghii Hook
American Journal of Clinical and Experimental Medicine
Volume 3, Issue 5-1, October 2015, Pages: 1-6
Received: Jun. 16, 2015;
Accepted: Jun. 17, 2015;
Published: Jul. 15, 2015
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Farzana Akther Sumi, Department of Pharmacy, North South University, Dhaka, Bangladesh
Biswajit Sikder, Department of Pharmacy, North South University, Dhaka, Bangladesh
Prawej Ansari, Department of Pharmacy, North South University, Dhaka, Bangladesh
Md. Reyad-ul- Ferdous, Department of Pharmacy, North South University, Dhaka, Bangladesh; Department of Pharmacy, Progati Medical Institute, Dhaka, Bangladesh
Anaytulla , Department of Pharmacy, North South University, Dhaka, Bangladesh
Mustafe Khalid Mohamed, Department of Pharmacy, North South University, Dhaka, Bangladesh
Md. Mustarek Uddin Meemo, Department of Pharmacy, North South University, Dhaka, Bangladesh
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Background: mussaenda roxburghii hook. Belongs to family rubiaceae, is a perennial shrub moist area of valley and grows in the foothills. Objective: the present investigation was carried out to phytochemicals and pharmacological activity such anti-inflammatory, cardioprotective properties of methanolic crude extract & its fractions such as dcm extract (fraction 1), ethyl acetate extract (fraction2). Materials and methods: residual methanolic extract (fraction 3) of mussaenda roxburghii leaves which were obtained by solvent-solvent extraction process from crude methanolic extract of mussaenda roxburghii. Anti-inflammatory activity was measured by observing the mean inhibition of protein denaturation. Cardioprotective activity was evaluated by thrombolytic assay. Phytochemical screenings have done by using usual procedures. Results: an ex-vivo anti-inflammatory test demonstrated that; mean inhibition of protein denaturation 0f 1000/kg of crude methanolic extract of leave of m. Roxburghii was 17.399%. Cardioprotective properties of different extracts of m. Roxburghii was demonstrated which revealed that after treatment of clot with mother methanolic extract, dcm extract (fraction 1), ethyl acetate extract (fraction 2), residual methanolic extract (fraction3) of leaves and methanolic extract of root % clot lysis was 30.56%,27.61%,46.35%,26.02%,49.90 % respectively. Different tests with crude extracts showed that different types of alkaloids, carbohydrates, tannins were present in the leaves of m. Roxburghii. Conclusion: in these investigations demonstrates that different extracts of m. Roxburghii has significant pharmacological activities.
Ex-vivo Anti-inflammatory, Cardioprotective Properties, Phytochemicals Investigation, Mean Inhibition of Protein Denaturation
To cite this article
Farzana Akther Sumi,
Md. Reyad-ul- Ferdous,
Mustafe Khalid Mohamed,
Md. Mustarek Uddin Meemo,
Potential Ex-vivo Anti-inflammatory, Cardioprotective Properties and Phytochemical Properties of Leaves of Mussaenda Roxburghii Hook, American Journal of Clinical and Experimental Medicine. Special Issue: Herbal Remedies as Alternative to Future Drugs Development and Treatment .
Vol. 3, No. 5-1,
2015, pp. 1-6.
Farnsworth nr, akerele o, bingel as, soejarta dd, eno z. Medicinal plants in therap. World health organ 1985; 63: 965–81.
Saha j, sarkar pk, chattopadhyay s. 2011. A survey of ethnomedicinal plants of darjeeling hills for their antimicrobial and antioxidant activities. Ind j nat prod res 2: 479 - 492.
Patil sa, joshi vg. 2011. Evaluation of antibacrerial and wound healing activity of leaves of mussaenda frondosa linn. Int j res pharmac biomed sc 2: 147 - 154.
Das hb, majumdar k, datta bk, ray d. 2009. Ethnobotanical uses of some medicinal plants by tripuri and reang tribes of tripura. Nat prod rad 8: 172 - 180.
Rahman ma. 2010. Indigenous knowledge of herbal medicines in bangladesh and treatment of skin diseases by tribal communities of the hill tracts districts. Bangladesh j bot 39: 169 – 177
Chandra du, ghosh r, chowdhury s, dinda b. 2012. New iridoid from aerial parts of mussaenda roxburghii. Nat prod commun 7: 1 - 2.
Van wagenen bc, larsen r, cardellina jh, ran dazzo d, lidert zc and swithenbank c: ulosantoin (1993) a potent insecticide from the sponge ulosaruetzleri. Journal of organic chemistry 58: 335-337.
Mizushima y and kobayashi m. Interaction of anti-inflammatory drugs with serum preoteins, especially with some biologically active proteins. J of pharma pharmacol. 1968; 20:169-173.
Reyad-ul-ferdous m, alam tt, islam ma, khan mzi, tasnim f, et al (2014) ex-vivo cardioprotective and cytotoxic screening of fruits of parmentiera ereifera seem. Biol med 6: 219. Doi: 10.4172/0974-8369.1000219.
Md. Reyad-ul-ferdous, sayma akhter, md. Zahirul islam khan, md. Eshak khan, md. Atiqul islam, md. Sharif ullah. Ex-vivo anti-inflammatory and antimicrobial activities of the leaves of bauhinia acuminata. American journal of life sciences. Vol. 2, no. 5, 2014, pp. 267-270. Doi: 10.11648/j.ajls.20140205.13.
Daginawala hf, prasad s, kashyap rs, deopujari jy, purohit hj and taori gm (2006) development of an in vitro model to study clot lysis activity of thrombolytic drugs. Thrombosis journal 4: 14.
Kawsar mh, sikder ma, rana ms, nimmi i and rashid ma (2011) studies of thrombolytic and cytotoxic properties of two asteraceous plants of bangladesh. Bangladesh pharmaceutical journal 14: 103-106.
Md. Reyad-ul-ferdous, nasir uddin, d.m. shamim shahjahan, mosharaf hossen, md. Saiful islam arman, ariful islam. Preliminaryin-vitro potential phytochemicals investigation of barks of ravenala madagascariensis sonnerat. Wjpps, 2014; 3(9), 1418-1423.
Egwaikhide, p.a., s.o. okeniyi and c.e. gimba, 2007. Screening for antimicrobial activity and phytochemical constituents of some nigerian medicinal plants. Adv. Biological res., 1: 155-158.
Harborne, j.b. phytochemical methods. A guide to modern techniques of plant analysis. 3rd edn., new york; chapman and hall int ed.1998.
Gani a. Methods of screening, practical phytochemistry,2nd edn, dhaka:chaya prokashoni; 2007.p. 12-17.