American Journal of Clinical and Experimental Medicine

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Effects of Preventive Long-Term Treatment with Strontium Ranelate and Zoledronic Acid on Bone Quality in Ovariectomized Rats

Received: 03 September 2016    Accepted: 18 September 2016    Published: 15 November 2016
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Abstract

The aim of this work was to study the effects on bone quality produced by long-range treatment with zoledronic acid (ZA) or strontium ranelate (SrR) in ovariectomized rats. Sixty 6-month-old female Wistar rats were divided: SHAM (n=15), simulated intervention; OVX (n=15), ovariectomized; OVX+ZA (n=15), ovariectomized treated with ZA (0.083 mg/kg i. v. at the beginning of the study); and OVX+SrR (n=15), ovariectomized treated with SrR (0.033 g/kg/day by oral gavage). Rats were sacrificed 8 months later. Femoral (F) and lumbar (L) bone mineral density (BMD), trabecular and cortical microstructure, biomechanical testing and Raman spectrometry were performed. FBMD and LBMD decreased in OVX rats with no changes with SrR. ZA treatment prevented changes to BMD. Ovariectomy produced a reduction in micro-CT parameters, while SrR treatment did not avoid these changes. ZA treatment increased micro-CT parameters with respect to the SHAM group. The microstructural parameters of the cortical region were not modified in any of the groups. Ovariectomy produced a decrease in biomechanical parameters that was maintained with SrR. ZA treatment produced an increase in these parameters being higher than those of the SHAM group. Ovariectomy and treatments did not produce differences in crystallinity or substitution index. Mineralization index (MI) decreased in the OVX group. SrR did not revert this effect though ZA avoided it. According to our results preventive treatment with SrR did not revert the alterations in bone quality due to ovariectomy in rats. Treatment with ZA not only reverted the effects of ovariectomy, but also improved bone quality with respect to control rats.

DOI 10.11648/j.ajcem.20160406.16
Published in American Journal of Clinical and Experimental Medicine (Volume 4, Issue 6, November 2016)
Page(s) 191-200
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Ovariectomized Rats, Zoledronic Acid, Strontium Ranelate

References
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Author Information
  • Biochemistry Investigation, Sanitary Research Institute Jimenez Diaz Foundation, Madrid, Spain

  • Biochemistry Investigation, Sanitary Research Institute Jimenez Diaz Foundation, Madrid, Spain

  • Biochemistry Investigation, Sanitary Research Institute Jimenez Diaz Foundation, Madrid, Spain

  • Internal Medicine, Sanitary Research Institute Jimenez Diaz Foundation, Madrid, Spain

  • Trabeculae, Limited Society, Technological Park of Galicia, Ourense, Spain

  • Traumatology and Orthopedic Surgery, Universitary Hospital of Santiago de Compostela, Santiago de Compostela, Spain

  • Biochemistry Investigation, Sanitary Research Institute Jimenez Diaz Foundation, Madrid, Spain

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    Marta Martín-Fernández, Marina Gómez-Chinchón, Luis Álvarez, Blanca Torrubia, Manuel Díaz-Curiel, et al. (2016). Effects of Preventive Long-Term Treatment with Strontium Ranelate and Zoledronic Acid on Bone Quality in Ovariectomized Rats. American Journal of Clinical and Experimental Medicine, 4(6), 191-200. https://doi.org/10.11648/j.ajcem.20160406.16

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    Marta Martín-Fernández; Marina Gómez-Chinchón; Luis Álvarez; Blanca Torrubia; Manuel Díaz-Curiel, et al. Effects of Preventive Long-Term Treatment with Strontium Ranelate and Zoledronic Acid on Bone Quality in Ovariectomized Rats. Am. J. Clin. Exp. Med. 2016, 4(6), 191-200. doi: 10.11648/j.ajcem.20160406.16

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    AMA Style

    Marta Martín-Fernández, Marina Gómez-Chinchón, Luis Álvarez, Blanca Torrubia, Manuel Díaz-Curiel, et al. Effects of Preventive Long-Term Treatment with Strontium Ranelate and Zoledronic Acid on Bone Quality in Ovariectomized Rats. Am J Clin Exp Med. 2016;4(6):191-200. doi: 10.11648/j.ajcem.20160406.16

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  • @article{10.11648/j.ajcem.20160406.16,
      author = {Marta Martín-Fernández and Marina Gómez-Chinchón and Luis Álvarez and Blanca Torrubia and Manuel Díaz-Curiel and David Guede and Jose Ramón Caeiro and Cristobalina Rodríguez-Álvarez and Concepción de la Piedra},
      title = {Effects of Preventive Long-Term Treatment with Strontium Ranelate and Zoledronic Acid on Bone Quality in Ovariectomized Rats},
      journal = {American Journal of Clinical and Experimental Medicine},
      volume = {4},
      number = {6},
      pages = {191-200},
      doi = {10.11648/j.ajcem.20160406.16},
      url = {https://doi.org/10.11648/j.ajcem.20160406.16},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajcem.20160406.16},
      abstract = {The aim of this work was to study the effects on bone quality produced by long-range treatment with zoledronic acid (ZA) or strontium ranelate (SrR) in ovariectomized rats. Sixty 6-month-old female Wistar rats were divided: SHAM (n=15), simulated intervention; OVX (n=15), ovariectomized; OVX+ZA (n=15), ovariectomized treated with ZA (0.083 mg/kg i. v. at the beginning of the study); and OVX+SrR (n=15), ovariectomized treated with SrR (0.033 g/kg/day by oral gavage). Rats were sacrificed 8 months later. Femoral (F) and lumbar (L) bone mineral density (BMD), trabecular and cortical microstructure, biomechanical testing and Raman spectrometry were performed. FBMD and LBMD decreased in OVX rats with no changes with SrR. ZA treatment prevented changes to BMD. Ovariectomy produced a reduction in micro-CT parameters, while SrR treatment did not avoid these changes. ZA treatment increased micro-CT parameters with respect to the SHAM group. The microstructural parameters of the cortical region were not modified in any of the groups. Ovariectomy produced a decrease in biomechanical parameters that was maintained with SrR. ZA treatment produced an increase in these parameters being higher than those of the SHAM group. Ovariectomy and treatments did not produce differences in crystallinity or substitution index. Mineralization index (MI) decreased in the OVX group. SrR did not revert this effect though ZA avoided it. According to our results preventive treatment with SrR did not revert the alterations in bone quality due to ovariectomy in rats. Treatment with ZA not only reverted the effects of ovariectomy, but also improved bone quality with respect to control rats.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Effects of Preventive Long-Term Treatment with Strontium Ranelate and Zoledronic Acid on Bone Quality in Ovariectomized Rats
    AU  - Marta Martín-Fernández
    AU  - Marina Gómez-Chinchón
    AU  - Luis Álvarez
    AU  - Blanca Torrubia
    AU  - Manuel Díaz-Curiel
    AU  - David Guede
    AU  - Jose Ramón Caeiro
    AU  - Cristobalina Rodríguez-Álvarez
    AU  - Concepción de la Piedra
    Y1  - 2016/11/15
    PY  - 2016
    N1  - https://doi.org/10.11648/j.ajcem.20160406.16
    DO  - 10.11648/j.ajcem.20160406.16
    T2  - American Journal of Clinical and Experimental Medicine
    JF  - American Journal of Clinical and Experimental Medicine
    JO  - American Journal of Clinical and Experimental Medicine
    SP  - 191
    EP  - 200
    PB  - Science Publishing Group
    SN  - 2330-8133
    UR  - https://doi.org/10.11648/j.ajcem.20160406.16
    AB  - The aim of this work was to study the effects on bone quality produced by long-range treatment with zoledronic acid (ZA) or strontium ranelate (SrR) in ovariectomized rats. Sixty 6-month-old female Wistar rats were divided: SHAM (n=15), simulated intervention; OVX (n=15), ovariectomized; OVX+ZA (n=15), ovariectomized treated with ZA (0.083 mg/kg i. v. at the beginning of the study); and OVX+SrR (n=15), ovariectomized treated with SrR (0.033 g/kg/day by oral gavage). Rats were sacrificed 8 months later. Femoral (F) and lumbar (L) bone mineral density (BMD), trabecular and cortical microstructure, biomechanical testing and Raman spectrometry were performed. FBMD and LBMD decreased in OVX rats with no changes with SrR. ZA treatment prevented changes to BMD. Ovariectomy produced a reduction in micro-CT parameters, while SrR treatment did not avoid these changes. ZA treatment increased micro-CT parameters with respect to the SHAM group. The microstructural parameters of the cortical region were not modified in any of the groups. Ovariectomy produced a decrease in biomechanical parameters that was maintained with SrR. ZA treatment produced an increase in these parameters being higher than those of the SHAM group. Ovariectomy and treatments did not produce differences in crystallinity or substitution index. Mineralization index (MI) decreased in the OVX group. SrR did not revert this effect though ZA avoided it. According to our results preventive treatment with SrR did not revert the alterations in bone quality due to ovariectomy in rats. Treatment with ZA not only reverted the effects of ovariectomy, but also improved bone quality with respect to control rats.
    VL  - 4
    IS  - 6
    ER  - 

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