American Journal of Clinical and Experimental Medicine

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HIF-1α Was a Key Regulator to Improve hBMSCs to Secrete Vascular Endothelial Cytokine with Astragaloside in Hypoxia

Received: 01 August 2019    Accepted: 06 September 2019    Published: 23 September 2019
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Abstract

This study aimed to assess the combined therapeutic efficacies with gene therapy and stem cell treatment and herb on the differentiation of hBMSCs into vascular endothelial cells under hypoxia condition. The third passage of hBMSCs were randomly divided into four groups, including control group (hBMSCs transfected with empty vectors), VEGF group (VEGF gene transfected to hBMSCs), AST group (cultured with AST), and VEGF + AST group (the intervention of VEGF group plus AST group). Each group was cultured at 37°C and 5% O2 for 2 weeks. Cell morphology was observed by inverted phase and were crowded and arranged irregularly in the control group and AST group, showing a fiber-like growth, while those in the VEGF group and VEGF plus AST group were mostly triangular or polygonal, exhibiting a colony-like growth, contrast microscope. CD31 was negative in the control group and AST group, while CD105 was positive in both groups, tested by flow cytometry assay. The positive rate of CD31 was significantly higher in the VEGF group than it in the VEGP + AST, and the positive rate of CD105 was lower in the VEGF group than it in the VEGF + AST group. The levels of VEGF and endothelial nitric oxide synthase (eNOS) by ELISA and the expression of endothelin and prostacyclin by west blot (WB) and RT-PCR were significantly higher in VEGF group and AST group and VEGF plus AST group, compared with control group. Further, the expression of endothelin and vWF and VEGFR-2 was highest in the VEGP + AST group and the expression of prostacyclin was lowest in VEGF group. AST can promote the secretion of VEGF from the differentiation of hBMSCs induced by VEGF gene transfected to hBMSCs by HIF-1α under hypoxia.

DOI 10.11648/j.ajcem.20190704.11
Published in American Journal of Clinical and Experimental Medicine (Volume 7, Issue 4, July 2019)
Page(s) 75-82
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

HIF-1α, Astragaloside, Hypoxia, Bone Mesenchymal Stem Cells, VEGF Gene Transfection, Vascular Endothelial Cytokine

References
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Author Information
  • Center of Research and Experiment, Public Health School, Gansu University of Chinese Medicine, Lanzhou, China

  • School of Chinese Medicine, Gansu University of Chinese Medicine, Lanzhou, China

  • School of Chinese Medicine, Gansu University of Chinese Medicine, Lanzhou, China

  • Center of Research and Experiment, Public Health School, Gansu University of Chinese Medicine, Lanzhou, China

  • Center of Research and Experiment, Public Health School, Gansu University of Chinese Medicine, Lanzhou, China

  • Center of Research and Experiment, Public Health School, Gansu University of Chinese Medicine, Lanzhou, China

  • Center of Research and Experiment, Public Health School, Gansu University of Chinese Medicine, Lanzhou, China

  • Center of Research and Experiment, Public Health School, Gansu University of Chinese Medicine, Lanzhou, China

  • Center of Research and Experiment, Public Health School, Gansu University of Chinese Medicine, Lanzhou, China

Cite This Article
  • APA Style

    Ji Hong Hu, Jin Hua, Jia Jia, Lu Juan, Jing Miao Zhao, et al. (2019). HIF-1α Was a Key Regulator to Improve hBMSCs to Secrete Vascular Endothelial Cytokine with Astragaloside in Hypoxia. American Journal of Clinical and Experimental Medicine, 7(4), 75-82. https://doi.org/10.11648/j.ajcem.20190704.11

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    ACS Style

    Ji Hong Hu; Jin Hua; Jia Jia; Lu Juan; Jing Miao Zhao, et al. HIF-1α Was a Key Regulator to Improve hBMSCs to Secrete Vascular Endothelial Cytokine with Astragaloside in Hypoxia. Am. J. Clin. Exp. Med. 2019, 7(4), 75-82. doi: 10.11648/j.ajcem.20190704.11

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    AMA Style

    Ji Hong Hu, Jin Hua, Jia Jia, Lu Juan, Jing Miao Zhao, et al. HIF-1α Was a Key Regulator to Improve hBMSCs to Secrete Vascular Endothelial Cytokine with Astragaloside in Hypoxia. Am J Clin Exp Med. 2019;7(4):75-82. doi: 10.11648/j.ajcem.20190704.11

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  • @article{10.11648/j.ajcem.20190704.11,
      author = {Ji Hong Hu and Jin Hua and Jia Jia and Lu Juan and Jing Miao Zhao and Shu Xia Wang and Yun Wang and Li Mei Jin and Jin Juan Li},
      title = {HIF-1α Was a Key Regulator to Improve hBMSCs to Secrete Vascular Endothelial Cytokine with Astragaloside in Hypoxia},
      journal = {American Journal of Clinical and Experimental Medicine},
      volume = {7},
      number = {4},
      pages = {75-82},
      doi = {10.11648/j.ajcem.20190704.11},
      url = {https://doi.org/10.11648/j.ajcem.20190704.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajcem.20190704.11},
      abstract = {This study aimed to assess the combined therapeutic efficacies with gene therapy and stem cell treatment and herb on the differentiation of hBMSCs into vascular endothelial cells under hypoxia condition. The third passage of hBMSCs were randomly divided into four groups, including control group (hBMSCs transfected with empty vectors), VEGF group (VEGF gene transfected to hBMSCs), AST group (cultured with AST), and VEGF + AST group (the intervention of VEGF group plus AST group). Each group was cultured at 37°C and 5% O2 for 2 weeks. Cell morphology was observed by inverted phase and were crowded and arranged irregularly in the control group and AST group, showing a fiber-like growth, while those in the VEGF group and VEGF plus AST group were mostly triangular or polygonal, exhibiting a colony-like growth, contrast microscope. CD31 was negative in the control group and AST group, while CD105 was positive in both groups, tested by flow cytometry assay. The positive rate of CD31 was significantly higher in the VEGF group than it in the VEGP + AST, and the positive rate of CD105 was lower in the VEGF group than it in the VEGF + AST group. The levels of VEGF and endothelial nitric oxide synthase (eNOS) by ELISA and the expression of endothelin and prostacyclin by west blot (WB) and RT-PCR were significantly higher in VEGF group and AST group and VEGF plus AST group, compared with control group. Further, the expression of endothelin and vWF and VEGFR-2 was highest in the VEGP + AST group and the expression of prostacyclin was lowest in VEGF group. AST can promote the secretion of VEGF from the differentiation of hBMSCs induced by VEGF gene transfected to hBMSCs by HIF-1α under hypoxia.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - HIF-1α Was a Key Regulator to Improve hBMSCs to Secrete Vascular Endothelial Cytokine with Astragaloside in Hypoxia
    AU  - Ji Hong Hu
    AU  - Jin Hua
    AU  - Jia Jia
    AU  - Lu Juan
    AU  - Jing Miao Zhao
    AU  - Shu Xia Wang
    AU  - Yun Wang
    AU  - Li Mei Jin
    AU  - Jin Juan Li
    Y1  - 2019/09/23
    PY  - 2019
    N1  - https://doi.org/10.11648/j.ajcem.20190704.11
    DO  - 10.11648/j.ajcem.20190704.11
    T2  - American Journal of Clinical and Experimental Medicine
    JF  - American Journal of Clinical and Experimental Medicine
    JO  - American Journal of Clinical and Experimental Medicine
    SP  - 75
    EP  - 82
    PB  - Science Publishing Group
    SN  - 2330-8133
    UR  - https://doi.org/10.11648/j.ajcem.20190704.11
    AB  - This study aimed to assess the combined therapeutic efficacies with gene therapy and stem cell treatment and herb on the differentiation of hBMSCs into vascular endothelial cells under hypoxia condition. The third passage of hBMSCs were randomly divided into four groups, including control group (hBMSCs transfected with empty vectors), VEGF group (VEGF gene transfected to hBMSCs), AST group (cultured with AST), and VEGF + AST group (the intervention of VEGF group plus AST group). Each group was cultured at 37°C and 5% O2 for 2 weeks. Cell morphology was observed by inverted phase and were crowded and arranged irregularly in the control group and AST group, showing a fiber-like growth, while those in the VEGF group and VEGF plus AST group were mostly triangular or polygonal, exhibiting a colony-like growth, contrast microscope. CD31 was negative in the control group and AST group, while CD105 was positive in both groups, tested by flow cytometry assay. The positive rate of CD31 was significantly higher in the VEGF group than it in the VEGP + AST, and the positive rate of CD105 was lower in the VEGF group than it in the VEGF + AST group. The levels of VEGF and endothelial nitric oxide synthase (eNOS) by ELISA and the expression of endothelin and prostacyclin by west blot (WB) and RT-PCR were significantly higher in VEGF group and AST group and VEGF plus AST group, compared with control group. Further, the expression of endothelin and vWF and VEGFR-2 was highest in the VEGP + AST group and the expression of prostacyclin was lowest in VEGF group. AST can promote the secretion of VEGF from the differentiation of hBMSCs induced by VEGF gene transfected to hBMSCs by HIF-1α under hypoxia.
    VL  - 7
    IS  - 4
    ER  - 

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