Lack of Association between ACE I/D and AGTR1 A1166C Gene Polymorphisms and Preeclampsia in Turkish Pregnant Women of Trakya Region
Journal of Gynecology and Obstetrics
Volume 2, Issue 4, July 2014, Pages: 49-53
Received: May 19, 2014;
Accepted: Jun. 10, 2014;
Published: Jun. 30, 2014
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Nevra Alkanli, Department of Biophysics, Faculty of Medicine, Trakya University, Edirne, Turkey
Tammam Sipahi, Department of Biophysics, Faculty of Medicine, Trakya University, Edirne, Turkey
Tulay Okman Kilic, Department of Obstetrics and Gynecology, Faculty of Medicine, Trakya University, Edirne, Turkey
Seralp Sener, Department of Biophysics, Faculty of Medicine, Trakya University, Edirne, Turkey
The aim of this study was to investigate the distribution of Angiotensin Converting Enzyme Insertion/Deletion (ACE I/D) and the distribution of Angiotensin II Type 1 Receptor A1166C (AGTR1 A1166C) gene polymorphisms in preeclamptic pregnant women comparing to control pregnant women in Turkish subjects of Trakya Region. We aimed to determine whether these polymorphisms are genetic risk factor for preeclampsia. The study included 75 preeclamptic pregnant women and 75 control pregnant women, which were categorized according to The World Health Organization Detecting Pre-eclampsia: A Practical Guide. The ACE I/D gene polymorphism was investigated using Polymerase Chain Reaction (PCR) method. The AGTR1 A1166C gene polymorphism was identified using PCR and followed by Restriction Fragment Length Polymorphism (RFLP) methods. The ACE I/D genotype distribution in preeclamptic pregnant women DD=36.0%, ID=44.0% and II=20.0% did not significantly differ from those in control pregnant women DD=38.7%, ID=50.7% and II=10.6% (P=0.279). The AGTR1 A1166C genotype distribution in preeclamptic pregnant women AA=57.3%, AC=33.4% and CC=9.3% also did not significantly differ from those in control pregnant women AA=70.7, AC=24.0% and CC=5.3% (P=0.223). This case-control study show that ACE I/D and AGTR1 A1166C gene polymorphisms are not genetic risk factors for preeclampsia in this population in Turkish pregnant women of Trakya Region.
Tulay Okman Kilic,
Lack of Association between ACE I/D and AGTR1 A1166C Gene Polymorphisms and Preeclampsia in Turkish Pregnant Women of Trakya Region, Journal of Gynecology and Obstetrics.
Vol. 2, No. 4,
2014, pp. 49-53.
Judi A Turner. Diagnosis and management of pre-eclampsia: an update. International Journal of Women’s Health. 2010; (2): 327-337.
Jie Yang, Jianyu Shang, Suli Zhang, Hao Li and Hui-rong Liu. The role of the renin–angiotensin–aldosterone system in preeclampsia: genetic poly-morphisms andmicroRNA. Journal of Molecular Endocrinology 2013; (50): 53-66.
Paula J. Williams, Fiona Broughton Pipkin. The genetic of pre-eclampsia and other hypertensive disorders of pregnancy. Best Practice & Research Clinical Obstetrics and Gynaecology. 2011; (25): 405-417.
Christiane Ruster and Gunter Wolf. Renin-Angiotensin-Aldosterone System and Progression of Renal Disease. Journal of the American Society of Nephrology. 2006; (17): 2985-2991.
Guo Deng Fu, Yu Lin Sun, Pavel Hamet, Tadashi Inagami. The angiotensin II type 1 receptor and receptor-associated proteins. Cell Research. (2001); 11 (3): 165-180.
Thway TM, Shlykov SG, Day MC, Sanborn BM, Gilstrap LC III, Xia Y, Kellems RE. Antibodies from preeclamptic patients stimulate increased intracellular Ca2+ mobilization through angiotensin receptor activation. Circulation. 2004; 110 (12): 1612-1619.
F. A. Sayed-Tabatabaei, B. A. Oostra, A. Isaacs, C. M. van Duijn, and J. C. M. Witteman. ACE polymor-phisms. Circulation Research. 2006; 98 (9): 1123-1133.
Bonnardeaux A, Davies E, Jeunemaitre X, Fery I, Charru A, Clauser E, et al. Angiotensin II type 1 receptor gene polymor-phisms in human essential hypertension. Hypertension. 1994; 24: 63-69.
Zarouk WA, Hussein IR, Esmaeil NN, Raslan HM, Reheim HAA, Moguib O, Emara NA, Aly AA, Hamed M. Asso-ciation of angiotensin converting enzyme gene (I/D) polymorphism with hypertension and type 2 diabetes. Bratislava Medical Journal. 2012; 113 (1): 14-18.
Bonnardeaux A, Davies E, Jeunemaitre X, Fery I, Charru A, Clauser E, Tiret L, Cambien F, Corvol P. Soubrier F. Angiotensin II type 1 receptor gene polymorphism in human essential hypertension. Hypertens. 1994;/24:/63-9.
Badaruddoza and Neha Sudhir. No Evidence for Association Between ACE Gene Insertion (I)/ Deletion (D) Polymorphism and Hypertension in North Indian Punjabi Population. Int J Hum Genet. 2012; 12 (3): 179-185.
Mary Esien Kooffreh, Chiaka Ijeoma Anumudu, Roseline Duke, Elza Cletus Okpako and P. Lava Kumar. Angiotensin II type 1 receptor A1166C gene polymorphism and essential hypertension in Calabar and Uyo cities, Nigeria. Indian J Hum Genet. 2013; 19 (2): 213-218.
Salimi S, Mokhtari M, Yaghmaei M, et al. Association of angiotensin-converting enzyme intron 16 insertion/deletion and angiotensin II type 1 receptor A1166C gene polymorphisms with preeclampsia in South East of Iran. J Biomed Biotechnol 2011; 2011: 941515.
Lu Shun Zhang, Han Qi Yang, Hao Jie Qin and Kui Zhang. Angiotensin II type I receptor A1166C polymorphism increases the risk of pregnancy hypertensive disorders: Evidence from a meta-analysis. Journal of the Renin-Angiotensin- Aldosterone System. 2012; DOI: 10.1177/1470320312467556.
T.Sipahi, M.Budak, S.Şen, A.Ay, S.Şener, “Association Be-tween ACE Gene Insertion (I) / Deletion (D) Polymorphism and Primary Hypertension in Turkish Patients of Trakya Region”, Biotechnol. & Biotechnol. Eq., 2006; 20 (2): 104-108.
T.Sipahi, B.Guldiken, S.Guldiken, S.Ustundag, N.Turgut, M.Budak, S.Cakina, H.Ozkan, S.Sener. The Associa-tion of Gene Polymorphisms of the Angiotensin-Converting Enzyme and Angiotensin II Receptor Type 1 with Ischemic Stroke in Turkish subjects of Trakya Region. Trakya Univ. Tip Fak. Derg., 2009; 1-8.
A.A.Basak, T.Sipahi, S.Ustundag, Z.Ozgen, M.Budak, S.Sen, S.Sener. Association of angiotensinogen T174M and M235T gene variants with development of hypertension in Turkish subjects of Trakya region. Biotechnol. & Biotechnol. Eq. 2008; 22 (4): 984-989.
Hassan A. Alsokary, Mostafa A. Kamel, Sameh S. Sadek, Nermine H. Zakaria, Iman A. Abdel Fatah. Study of angiotensin converting enzyme and genotype among Egyptian preeclampsia patients. American Journal of Molecular Biology, 2014, (4): 26-36.
Kim YJ, Park MH, Park HS, et al. Associa-tions of polymorphisms of the angiotensinogen M235 polymorphism and angioten-sin-converting-enzyme intron 16 insertion/deletion polymorphism with preeclampsia in Korean women. Eur J Obstet Gyn R B 2004; 116: 48–53.
Serrano NC, Diaz LA, Paez MC, Mesa CM, Cifuentes R, Monterrosa A, Gonzalez A, Smeeth L, Hingorani AD, Casas JP. Angiotensin-converting enzyme I/D polymorphism and preeclampsia risk: evidence of small-study bias. PLoS Medicine. 2006; 3 (12): 2304-2316.
Bouba I, Makrydimas G, Kalaitzidis R, Lolis DE, Siamopoulos KC, Georgiou I. Interaction between the polymorphisms of the renin-angiotensin system in preec-lampsia. European Journal of Obstetrics & Gynecology and Reproductive Biology 2003; 110: 8-11.
Roberts CB, Rom L, Moodley J, et al. Hypertension-related gene polymorphisms in pre-eclampsia, eclampsia and gestational hypertension in Black South African women. J Hyper-tens 2004; 22 (5): 945-948.
Ceyhun Bereketoglu, Mulkiye Kasap and Ayfer Pazarbasi. Stu-dies on Angiotensin-Converting Enzyme Insertion/Deletion Polymorphism and Genotype Distri-butions in Turkish Preeclampsia Patients. Journal of Pregnancy. 2012; DOI: 10.1155/2012/108206.
Aggarwal S, Dimri N, Tandon I, et al. Preeclampsia in North Indian women: the contribution of genetic polymorphisms. J Obstet Gynaecol Res 2011; 37: 1335–1341.
Uma R, Forsyth SJ, Struthers AD, Fraser CG, Godfrey V, Murphy DJ. Polymor-phisms of the angiotensin converting enzyme gene in early-onset and late-onset pre-eclampsia. J Matern Fetal Neonatal Med 2010; 23: 874–879.
Chen Z, Xu F, Wei Y, Liu F, Qi H. Angioten-sin converting enzyme insertion/deletion polymorphism and risk of pregnancy hypertensive dis-orders: a meta-analysis. Journal of the Renin-Angiotensin-Aldosterone System. 2012; 13 (1): 184-195.
Hyunah Choi, Ja Young Kang, Hong Sun Yoon, Seung Suk Han, Chang Sun Whang, In Gul Moon, Hyun-Ho Shin, Jeong Bae Park. Association of Angiotensin-Converting Enzyme and Angiotensinogen Gene Polymorphisms with Preeclampsia. J Korean Med Sci. 2004; 19: 253-257.
Roh CR, Kim DK, Yoon BK, Yang SH, Chung JH, Bae DS, Lee JH. A common genetic variant of the angiotensin converting enzyme (ACE) gene and pregnancy induced hypertensive disorders. Korean J Obstet Gynecol 1997; 40: 1189-99.
Syed Ali Akbar, Nuzhat P. Khawaja, Paul R. Brown, Rakhshanda Tayyeb, Jacqueline Bamfo1&Kypros H. Nicolaides. Angiotensin II type 1 and 2 receptors gene polymorphisms in pre-eclampsia and normal pregnancy in three different populations. Acta obstetricia et gynecologica Scandinavica. 2009; 88 (5): 606-611.
Linlu Zhao, Andrew T. Dewan, and Michael B. Bracken. Association of maternal AGTR1 polymorphisms and preeclampsia: a systematic review and meta-analysis. J Matern Fetal Neonatal Med. 2012; 25 (12): 2676–2680.