The Balance of Matrix Metalloproteinase-2 (Mmp-2) and Tissue Inhibitor of Matrix Metalloproteinase-2 (Timp-2) on Severe Endometriosis
Journal of Gynecology and Obstetrics
Volume 3, Issue 6, November 2015, Pages: 111-114
Received: Nov. 2, 2015;
Accepted: Nov. 17, 2015;
Published: Dec. 10, 2015
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Samrichard Rambulangi, Department of Obstetrics and Gynecology, Hasanuddin University, Makassar, Indonesia
Nusratuddin Abdullah, Department of Obstetrics and Gynecology, Hasanuddin University, Makassar, Indonesia
Irawan Yusuf, Department of Physiology, Hasanuddin University, Makassar, Indonesia
Syahrul Rauf, Department of Obstetrics and Gynecology, Hasanuddin University, Makassar, Indonesia
The objective of this study is to examine the level balance of serum and peritoneal fluid MMP-2 and TIMP-2 in patients with severe endometriosis. The method of this study was a cross sectional study with twenty patients were diagnosed as severe endometriosis based on laparoscopy and laparotomy with five controls. Serum samples were taken before surgery, peritoneal fluid samples were taken during surgery, and MMP-2 and TIMP-2 examination was conducted in the end of the study by using ELISA method. The mean level of serum, peritoneal fluid MMP-2 and TIMP-2 were compared by using unpaired t-test. Based on this study, we found that MMP-2 level of serum and peritoneal fluid was significantly different in the case compared to the control, 5.848±3.016 vs. 1.600±0.063 ng/mL and 1.977±1.883 vs. 0.573±0.084 ng/mL respectively. When TIMP-2 level of serum and peritoneal fluid compared to the control, the significant difference was only found in serum (p < 0.05), 0.853±0.343 vs. 0.637±0.116 ng/mL and 1.339±2.141 vs. 0.105±0.028 ng/mL respectively. This study showed that there was an increase of MMP-2 level of serum and peritoneal fluid and low level of serum and peritoneal TIMP-2 in severe endometriosis. Serum and peritoneal fluid MMP-2 as well as TIMP-2 in serum had a significant relationship with the incidence of severe endometriosis. Meanwhile, TIMP-2 in peritoneal fluid had no significant relationship with the incidence of severe endometriosis.
The Balance of Matrix Metalloproteinase-2 (Mmp-2) and Tissue Inhibitor of Matrix Metalloproteinase-2 (Timp-2) on Severe Endometriosis, Journal of Gynecology and Obstetrics.
Vol. 3, No. 6,
2015, pp. 111-114.
Situmorang H, Mutia K. Endometriosis. In: Hestiantoro A, Natadisastra RM, Sumapraja K, Wiweko B, Pratama G, Situmorang H, et al., editors. Best Practice on IMPERIAL. 1 ed. Jakarta: CV Sagung Seto; 2012. p. 65-76.
Annas JY. Perbandingan Ekspresi Prostaglandin E2 (PGE2), Matrix Metalloproteinase 9 (MMP-9) dan Luas implan lesi Endometriosis akibat pemberian Kurkumin dosis 240 mg/Kg BB, 500 mg/KgBB dan 1000/Kg BB pada mencit model Endometriosis. [Laporan Penelitian]. Surabaya: UNAIR; 2013.
Hadisaputra W. Kombinasi Petanda Biologis (IL-6, TNF-α, MMP-2, VEGF) dan gejala serta tanda klinis sebagai model prediktor diagnosis endometriosis perempuan masa reproduksi [Disertasi]. Jakarta: Universitas Indonesia, Jakarta; 2012.
Irfan, Rambulangi J, Bandaso R. Tinjauan kasus Endometriosis di bagian patologi anatomi fakutas kedokteran Unhas. Fakultas Kedokteran Universitas Hasanuddin, 2010.
Naruse K, Lash GE, Innes BA, Otun HA, Searle RF, Robson SC, et al. Localization of matrix metalloproteinase (MMP-2), MMP-9 ang tissue inhibitors for MMPs (TIMPs) in uterine natural killer cells in early human pregnancy. Human Reproduction. 2009; 24(3): 553-61.
Aresu L, Benali S, Giannuzzi D, Mantovani R, Castagnaro M, Falomo ME. The role of inflammation and matrix metalloproteinases in equine endometriosis. Veterinary Science. 2012; 13(2): 171-77.
Londero AP, Calcagno A, Grassi T, Marzinotto S, Orsaria M, Beltrami CA, et al. Survivin, MMP-2, MT1-MMP, and TIMP-2: their impact on survival, implantation, and proliferation of endometriotic tissues. Virchows Arch. 2012; 461: 589-99.
Artini, P., Ruggiero, M., Papini, F., Simi, G., Cela, V. & Genazzani, A. 2012. Endometrisis and angiogenic factors. In: Chaudhury, K. (ed.) Endometriosis-Basic concepts and Current Research Trends. First ed. Rijeka, Croatia: InTech Europe.
Jana, S., Rudra, D. S., Paul, S. & Swarnakar, S. 2012. Curcumin delays endometriosis development by inhibiting MMP-2 activity. Indian Journal of Biochemistry and Biophysics, 49, 342-348.
Speroff L, Fritz A. Endometriosis. Clinical Gynecologic Endocrinology and Fertility. Eight ed. Philadelphia: Lippincott Williams and Wilkins 2011. p. 1221-48.
Jana S, Rudra DS, Paul S, Swarnakar S. Curcumin delays endometriosis development by inhibiting MMP-2 activity. Indian Journal of Biochemistry and Biophysics. 2012; 49: 342-8.
Bozzuto G, Ruggieri P, Molinari A. Molecular aspect s of tumor cell migration and invasion. Ann Ist Super Sanita. 2010; 46(1): 66-80.
Li, S., Shen, X., Yang, Z., Wu, A., Tang, Z., Li, M. & Li, Y. 2014. Clinical significance of MMP-2 overexpression in endometrial adenocarcinoma. Nan Fang Yi Ke Da Xue Xue Bao, 34, 423-5.
Weigel, M. T., Kramer, J., Wenners, A., Alkatout, I., Jonat, W., Maass, N. & Mundhenke, C. 2012. Differential expression of MMP-2, MMP-9 and PCNA in endometriosis and endometrial carcinoma. European Journal of Obstetrics & Gynecology and Reproductive Biology, 160, 74-8.
Londero, A. P., Calcagno, A., Grassi, T., Marzinotto, S., Orsaria, M., Beltrami, C. A., Marchesoni, D. & Mariuzzi, L. 2012. Survivin, MMP-2, MT1-MMP, and TIMP-2 : their impact on survival, implantation, and proliferation of endometriotic tissues. Virchows Arch, 461, 589-99.